FastQCFastQC Report
Wed 25 May 2016
SRR1033044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3957918
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31300.035.1485061
GTATCAA48900.032.3047071
TCAACGC48700.030.6942214
ATCAACG50150.029.9942153
CAACGCA52500.028.5617185
GTACATG117650.027.8532711
AACGCAG55550.027.0723196
TACATGG119900.026.0711922
TATCAAC61550.024.9734272
ACATGGG122900.024.631593
ACGCAGA70050.021.4013987
CATGGGG92050.020.7329314
CGCAGAG73550.020.014538
TATACTG24500.017.6512185
GAGTACA87400.017.482651
TACACCG5700.017.3179865
AGTACAT87450.016.7707792
GCAGAGT86050.016.5633039
GGACCGT5205.456968E-1215.3640316
AGAGTAC74150.015.3411245
Produced by FastQC (version 0.11.5)