FastQCFastQC Report
Wed 25 May 2016
SRR1033044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3957918
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45770.11564160753204084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45750.050.8062171
GTATCAA84550.043.1049271
TCAACGC101400.035.642664
ATCAACG102750.035.1295053
CAACGCA104950.034.3909385
AACGCAG109150.033.0245446
TATCAAC109950.032.83112
ACGCAGA126350.028.380147
CGCAGAG129550.027.5706448
GTACATG109350.024.9212651
GCAGAGT147250.023.873559
TACATGG112850.023.3015372
ACATGGG113600.022.299913
AGAGTAC135950.020.3613610-11
CAGAGTA141900.019.7559910-11
GTGGTAT24750.019.2400951
GAGTACT94800.019.21041912-13
CATGGGG85150.019.0973534
AGTACTT97000.018.4597912-13
GAGTACA83950.017.9155861
Produced by FastQC (version 0.11.5)