FastQCFastQC Report
Wed 25 May 2016
SRR1033043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3134947
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36450.035.083831
GGTATCA23200.034.6532331
TCAACGC35350.033.77784
ATCAACG35350.033.1128853
CAACGCA37000.032.143935
AACGCAG39350.030.2199336
GTACATG79050.028.1911011
TATCAAC44000.027.0305522
TACATGG79900.026.7702052
CGCAGAG47750.024.5323528
ACGCAGA48000.024.4795047
ACATGGG82600.024.30163
CATGGGG59150.021.2199124
GCAGAGT61350.017.9481079
GAGTACA59950.016.9394841
CTGTCGC9100.016.5472559
GTCGTAT1750.00232488416.1193981
TATACTG20100.015.6696345
GTGTAGG12100.015.542121
CTGTCCG4008.743882E-915.2932829
Produced by FastQC (version 0.11.5)