##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033043_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3134947
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30053299146684	33.0	31.0	34.0	28.0	34.0
2	31.452164901033413	33.0	31.0	34.0	28.0	34.0
3	31.506924359486778	34.0	31.0	34.0	28.0	34.0
4	34.89782379096042	37.0	35.0	37.0	32.0	37.0
5	34.96715032183957	37.0	35.0	37.0	32.0	37.0
6	34.96644217589643	37.0	35.0	37.0	32.0	37.0
7	34.91204604096975	37.0	35.0	37.0	32.0	37.0
8	34.89965508188815	37.0	35.0	37.0	32.0	37.0
9	36.48622416902104	39.0	37.0	39.0	32.0	39.0
10-11	36.483184245220095	39.0	37.0	39.0	32.0	39.0
12-13	36.41846177941764	39.0	37.0	39.0	32.0	39.0
14-15	37.76335038518992	40.0	38.0	41.0	32.0	41.0
16-17	37.74607832285522	40.0	38.0	41.0	32.0	41.0
18-19	37.73654259545695	40.0	38.0	41.0	32.0	41.0
20-21	37.75841011028257	40.0	38.0	41.0	32.0	41.0
22-23	37.70540203709983	40.0	38.0	41.0	32.0	41.0
24-25	37.70457666429448	40.0	38.0	41.0	32.0	41.0
26-27	37.60802846108722	40.0	38.0	41.0	32.0	41.0
28-29	37.61449571555755	40.0	38.0	41.0	32.0	41.0
30-31	37.75906020101775	40.0	38.0	41.0	32.0	41.0
32-33	37.744206999352784	40.0	38.0	41.0	32.0	41.0
34-35	37.69750764526482	40.0	38.0	41.0	32.0	41.0
36-37	37.61424882143143	40.0	38.0	41.0	31.5	41.0
38-39	37.51967034849393	40.0	38.0	41.0	31.0	41.0
40-41	37.44614390610113	40.0	38.0	41.0	31.0	41.0
42-43	37.32006458163407	40.0	38.0	41.0	31.0	41.0
44-45	37.170987260709666	40.0	38.0	41.0	30.5	41.0
46-47	37.03504668499978	40.0	37.0	41.0	30.0	41.0
48-49	36.91514258454768	40.0	37.0	41.0	30.0	41.0
50-51	36.788289243805394	40.0	37.0	41.0	30.0	41.0
52-53	36.62751507441753	40.0	36.5	41.0	30.0	41.0
54-55	36.46874620208891	40.0	36.0	41.0	30.0	41.0
56-57	36.29426717580871	40.0	36.0	41.0	30.0	41.0
58-59	36.09776959546684	39.0	35.0	41.0	29.0	41.0
60-61	35.876738586011186	39.0	35.0	41.0	29.0	41.0
62-63	35.62619272351335	39.0	35.0	41.0	28.5	41.0
64-65	35.3518601749886	38.5	35.0	40.0	28.0	41.0
66-67	35.055950387678	38.0	34.5	40.0	28.0	41.0
68-69	34.743010487896605	37.0	34.0	40.0	28.0	41.0
70-71	34.40889447253814	37.0	34.0	39.5	27.5	41.0
72-73	34.057952654382994	36.0	34.0	39.0	27.0	41.0
74-75	33.70169703028472	36.0	34.0	39.0	26.0	40.5
76-77	33.33539402739504	35.0	33.5	38.5	26.0	40.0
78-79	32.944956326215404	35.0	33.0	37.0	26.0	39.0
80-81	32.58971172399406	35.0	33.0	37.0	26.0	39.0
82-83	32.3568422368863	35.0	33.0	36.5	25.5	39.0
84-85	32.1246998753089	35.0	33.0	36.0	25.0	37.0
86-87	31.943445774362374	35.0	33.0	36.0	25.0	37.0
88-89	31.7174515231039	35.0	33.0	35.0	25.0	37.0
90-91	31.49843841060152	35.0	33.0	35.0	24.5	36.0
92-93	31.269498655001186	35.0	33.0	35.0	24.0	36.0
94-95	31.060831171946447	35.0	32.5	35.0	23.0	36.0
96-97	30.873817324503413	35.0	32.0	35.0	20.5	35.5
98-99	30.687617685402657	35.0	32.0	35.0	19.5	35.0
100	30.539032398314866	35.0	32.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.024182586896312586
1101	2	0.08372650260490744
1101	3	0.07478153082148253
1101	4	0.02257878482929243
1101	5	0.02254763945739313
1101	6	0.13184295149194725
1101	7	0.19348550959643518
1101	8	0.226034346836947
1101	9	0.25780812087592864
1101	10-11	0.20952435832703742
1101	12-13	0.2220039746297786
1101	14-15	0.34282766338711923
1101	16-17	0.3210247746718622
1101	18-19	0.339310234537038
1101	20-21	0.33790468331559254
1101	22-23	0.3514753507961714
1101	24-25	0.36272586880981095
1101	26-27	0.25066373149959986
1101	28-29	0.30135444348911733
1101	30-31	0.3231904080242387
1101	32-33	0.3236986745257937
1101	34-35	0.3434837585851298
1101	36-37	0.3560920385031636
1101	38-39	0.4734956607797187
1101	40-41	0.4349471550878192
1101	42-43	0.4122422788480691
1101	44-45	0.42058193997499416
1101	46-47	0.3779196409001031
1101	48-49	0.36779085765074626
1101	50-51	0.3381029712402679
1101	52-53	0.244239445011722
1101	54-55	0.3011728898552306
1101	56-57	0.3950740209904353
1101	58-59	0.38594370799059163
1101	60-61	0.3650428297140209
1101	62-63	0.34647776283881626
1101	64-65	0.3813266121263439
1101	66-67	0.3480580660726744
1101	68-69	0.3962806763936584
1101	70-71	0.43200249546157465
1101	72-73	0.32580183519062444
1101	74-75	0.38532466914134034
1101	76-77	0.3766891539715047
1101	78-79	0.420933009543063
1101	80-81	0.3954573070480478
1101	82-83	0.3248466589598067
1101	84-85	0.3731455321385724
1101	86-87	0.36338208997632293
1101	88-89	0.36831529131201535
1101	90-91	0.33756154929647764
1101	92-93	0.3232286525385817
1101	94-95	0.33168265968681254
1101	96-97	0.37811975819978016
1101	98-99	0.38230406297342157
1101	100	0.4116050381375871
1102	1	-0.019392192209075176
1102	2	-0.01468956975780955
1102	3	0.05105401259049103
1102	4	-0.14684071190240644
1102	5	0.02555917526758833
1102	6	0.06331401446659157
1102	7	0.06490864008208774
1102	8	0.02339273969201372
1102	9	0.09511919775172117
1102	10-11	0.10775070553206945
1102	12-13	0.11294060816246798
1102	14-15	0.15731983177281705
1102	16-17	0.17885654880644353
1102	18-19	0.09343930838188896
1102	20-21	0.19528943081947148
1102	22-23	0.1603250281446691
1102	24-25	0.2390701739611174
1102	26-27	0.1368542106258417
1102	28-29	0.13210836865808062
1102	30-31	0.2048620421608831
1102	32-33	0.19354245552967342
1102	34-35	0.08098059897282184
1102	36-37	0.1452715736685377
1102	38-39	0.18280396427512358
1102	40-41	0.10935341626919381
1102	42-43	0.11308470512892654
1102	44-45	0.13603810122471316
1102	46-47	0.14562528627961058
1102	48-49	0.0986519413423963
1102	50-51	0.08545123735235194
1102	52-53	0.04551472961658476
1102	54-55	0.0723312750838403
1102	56-57	0.12192059889339646
1102	58-59	0.032733815886643924
1102	60-61	0.08839790757348709
1102	62-63	0.12352775469859978
1102	64-65	0.08113341624728321
1102	66-67	0.028276244295589947
1102	68-69	0.10767877658470582
1102	70-71	0.0022795800464834315
1102	72-73	0.0802898546942501
1102	74-75	0.18141813247144967
1102	76-77	0.11930656425165154
1102	78-79	0.07899194467550785
1102	80-81	0.10491049032748379
1102	82-83	0.09912098706125505
1102	84-85	0.0747785750501535
1102	86-87	0.06807507612791497
1102	88-89	0.08975650608276453
1102	90-91	0.07412506247026229
1102	92-93	0.058501434113978235
1102	94-95	0.1413905984824666
1102	96-97	0.1267229640959826
1102	98-99	0.12094395804803071
1102	100	0.048661542166705374
1103	1	0.13747912116224725
1103	2	0.20950004658895693
1103	3	0.18853327708226075
1103	4	0.17135919562909407
1103	5	0.14128363214521045
1103	6	0.2074286415915907
1103	7	0.2490266226041129
1103	8	0.18315027974381337
1103	9	0.2509851925586588
1103	10-11	0.3012582724075372
1103	12-13	0.36675720970452375
1103	14-15	0.37664802406568043
1103	16-17	0.37609488148626014
1103	18-19	0.39284248989650905
1103	20-21	0.34698523936936
1103	22-23	0.3131719784724396
1103	24-25	0.3228442282886377
1103	26-27	0.20615275057294014
1103	28-29	0.2672949410875134
1103	30-31	0.3032111022920674
1103	32-33	0.19277529862389287
1103	34-35	0.21658627089432514
1103	36-37	0.3096341685349131
1103	38-39	0.27703323413163616
1103	40-41	0.2748627635859435
1103	42-43	0.2897549742954908
1103	44-45	0.24514719129436457
1103	46-47	0.23419720069617256
1103	48-49	0.11757774610120464
1103	50-51	0.18463752106157472
1103	52-53	0.18621852414143092
1103	54-55	0.18154896036710966
1103	56-57	0.1750098400989799
1103	58-59	0.2217109465149818
1103	60-61	0.13181924854880123
1103	62-63	0.14553389195384625
1103	64-65	0.0778714956466402
1103	66-67	0.09328592357556431
1103	68-69	0.12383751677619159
1103	70-71	0.058888899336565714
1103	72-73	0.054421434377452726
1103	74-75	0.09584786779175403
1103	76-77	0.04934052138970202
1103	78-79	0.14967117197179647
1103	80-81	0.17855917895899154
1103	82-83	0.1695498601225509
1103	84-85	0.1848646969069918
1103	86-87	0.13463760193553398
1103	88-89	0.10458641801659496
1103	90-91	0.1357354514557194
1103	92-93	0.15814697153348334
1103	94-95	0.131311564576702
1103	96-97	0.2295062204047511
1103	98-99	0.22265524304154027
1103	100	0.23662002368048363
1104	1	0.03341952378480073
1104	2	0.15084109653807332
1104	3	0.08517878438250648
1104	4	0.06665021707828345
1104	5	0.03698806596171522
1104	6	0.20181971288063494
1104	7	0.14691034352710375
1104	8	0.20549120236939444
1104	9	0.21245659192527455
1104	10-11	0.27401912922957194
1104	12-13	0.2753594556419259
1104	14-15	0.3198795483774646
1104	16-17	0.29094742121727535
1104	18-19	0.31847301380017967
1104	20-21	0.33334410994420693
1104	22-23	0.3354854284764741
1104	24-25	0.23989769078843182
1104	26-27	0.220528169472054
1104	28-29	0.23201013327601316
1104	30-31	0.25579857185037724
1104	32-33	0.24877251742955053
1104	34-35	0.2523634845883649
1104	36-37	0.27601343889321583
1104	38-39	0.350112041798063
1104	40-41	0.38244119130524723
1104	42-43	0.3189737805735007
1104	44-45	0.3781795087477491
1104	46-47	0.3029246719791132
1104	48-49	0.23295878747077126
1104	50-51	0.2894761579339118
1104	52-53	0.3538006594972458
1104	54-55	0.35094802439901684
1104	56-57	0.3726639116971455
1104	58-59	0.3292702290079248
1104	60-61	0.31891535042506547
1104	62-63	0.29661821220025786
1104	64-65	0.3007568742660567
1104	66-67	0.3035749106498855
1104	68-69	0.3791377870818806
1104	70-71	0.29482634145339404
1104	72-73	0.23120394055918325
1104	74-75	0.20199351657988984
1104	76-77	0.18991683628539846
1104	78-79	0.2963258627545926
1104	80-81	0.211223347718402
1104	82-83	0.2089389063758631
1104	84-85	0.15307490464359574
1104	86-87	0.12345624496547813
1104	88-89	0.2341684699428086
1104	90-91	0.24348657534276796
1104	92-93	0.29226021601429153
1104	94-95	0.28706887657446956
1104	96-97	0.2662887919604202
1104	98-99	0.21589736727133513
1104	100	0.22192707419804236
1105	1	0.0037662838617400496
1105	2	0.055336072689165405
1105	3	0.034094993285773256
1105	4	0.05449140192624213
1105	5	0.05288850107037746
1105	6	0.079063588974428
1105	7	0.15308560376178804
1105	8	0.13182795504545197
1105	9	0.16646412253866316
1105	10-11	0.2482530942420027
1105	12-13	0.23323170844722796
1105	14-15	0.19997760262501174
1105	16-17	0.12931683996831822
1105	18-19	0.21253471922562284
1105	20-21	0.18908853692664707
1105	22-23	0.16866180031696842
1105	24-25	0.14998268784479052
1105	26-27	0.17360344022326046
1105	28-29	0.2087725100877762
1105	30-31	0.2501756745397614
1105	32-33	0.21285025317401818
1105	34-35	0.18428927531751071
1105	36-37	0.23281214683574092
1105	38-39	0.2746237362647008
1105	40-41	0.31032121847128025
1105	42-43	0.2734246266618072
1105	44-45	0.2705872950992685
1105	46-47	0.27249765710757856
1105	48-49	0.23737788743371624
1105	50-51	0.24902228171234952
1105	52-53	0.3439099723164887
1105	54-55	0.33891304862140714
1105	56-57	0.29806732337362263
1105	58-59	0.35158178430270226
1105	60-61	0.2870799083823883
1105	62-63	0.25085364166243096
1105	64-65	0.22384507935192488
1105	66-67	0.20888268098092055
1105	68-69	0.2213783411353063
1105	70-71	0.17394785834812865
1105	72-73	0.2268670807522284
1105	74-75	0.2449151134787968
1105	76-77	0.2962348803702497
1105	78-79	0.27308561043678736
1105	80-81	0.2642239954179928
1105	82-83	0.3005185414821625
1105	84-85	0.3152741887340724
1105	86-87	0.22483044430952148
1105	88-89	0.2651237432887399
1105	90-91	0.24182019861749282
1105	92-93	0.2583026486913731
1105	94-95	0.30512357622958675
1105	96-97	0.3130346912554245
1105	98-99	0.24840257393074694
1105	100	0.27412065283162335
1106	1	-0.035293763774514986
1106	2	-0.012915138991520081
1106	3	0.0068352823338209134
1106	4	0.0305052595758184
1106	5	0.033690073010738786
1106	6	0.10601434586053671
1106	7	0.06394829426712079
1106	8	0.09786032753070373
1106	9	0.1363596346283984
1106	10-11	0.12224756966899264
1106	12-13	0.12973979923435053
1106	14-15	0.1557914057477916
1106	16-17	0.14950244582733774
1106	18-19	0.1480557535915068
1106	20-21	0.11795776878037856
1106	22-23	0.11361895849977088
1106	24-25	-0.045918138740383085
1106	26-27	-0.005067455071284144
1106	28-29	-0.03213421267427208
1106	30-31	0.027546679914941308
1106	32-33	-0.02477314405090425
1106	34-35	-0.020398535422209818
1106	36-37	0.017657688476376165
1106	38-39	0.04181452310320566
1106	40-41	0.08115017518876755
1106	42-43	0.0297077197130875
1106	44-45	-6.876095955448136E-4
1106	46-47	-0.044032155699312625
1106	48-49	-0.027526928757751534
1106	50-51	-0.01276813464270532
1106	52-53	-0.02702794462911129
1106	54-55	-0.08061039276648785
1106	56-57	-0.022479359128226406
1106	58-59	-0.04447246892758727
1106	60-61	0.0161943474332773
1106	62-63	-0.014280135728185428
1106	64-65	-0.017897401972177818
1106	66-67	0.06884094090381154
1106	68-69	0.06081664509046192
1106	70-71	0.011691466621641666
1106	72-73	-0.007393231488379115
1106	74-75	0.036611961288343764
1106	76-77	0.06723724444307067
1106	78-79	0.05747719781084726
1106	80-81	0.011213443922372335
1106	82-83	0.04762570344635719
1106	84-85	-0.03916383978606319
1106	86-87	-0.01828216286504869
1106	88-89	-0.009098572923733173
1106	90-91	-0.035232375859671095
1106	92-93	-0.012644675060556665
1106	94-95	-0.02886828023048693
1106	96-97	-0.07377414189911491
1106	98-99	-0.010741331999323478
1106	100	-0.06787142397647017
1107	1	-0.1744189649748371
1107	2	-0.09821691617265671
1107	3	-0.09855242225838623
1107	4	-0.09924461788395433
1107	5	-0.11864340498016901
1107	6	-0.08904944056707365
1107	7	-0.003262641467500771
1107	8	-0.00909038124414252
1107	9	-0.03471111913482616
1107	10-11	0.013782886310380604
1107	12-13	0.04260481480563527
1107	14-15	0.0908437330261691
1107	16-17	0.06428191190715893
1107	18-19	0.038313531772686815
1107	20-21	0.06752049364288837
1107	22-23	0.05838448153416209
1107	24-25	-0.03365675452000261
1107	26-27	-0.08208424304906714
1107	28-29	-0.06651996702301233
1107	30-31	0.006570723462949957
1107	32-33	-0.040233135560548305
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2216	54-55	-0.9072496724257348
2216	56-57	-0.9414055061405264
2216	58-59	-0.8803659198414238
2216	60-61	-0.8965085507793091
2216	62-63	-0.9232371984629424
2216	64-65	-0.8542479607743871
2216	66-67	-0.9160190143790103
2216	68-69	-0.9080070415020671
2216	70-71	-0.9101848718814978
2216	72-73	-0.9012137898286028
2216	74-75	-0.9793699257245265
2216	76-77	-0.8917410950504276
2216	78-79	-0.9401955914085605
2216	80-81	-0.9061869401039644
2216	82-83	-1.1356738052961468
2216	84-85	-0.9754209688931539
2216	86-87	-0.9675406335803132
2216	88-89	-1.0175619173516282
2216	90-91	-1.0641645309736276
2216	92-93	-1.2103753514571913
2216	94-95	-1.0853717716850753
2216	96-97	-0.9532207000902915
2216	98-99	-0.9355501023419599
2216	100	-0.9133214825601037
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50209.0
3	9294.0
4	5292.0
5	4300.0
6	3571.0
7	3834.0
8	4458.0
9	4944.0
10	5703.0
11	6131.0
12	6846.0
13	7363.0
14	7795.0
15	8212.0
16	8157.0
17	8293.0
18	8189.0
19	8118.0
20	8212.0
21	8580.0
22	9751.0
23	13239.0
24	19717.0
25	25015.0
26	24425.0
27	22974.0
28	25913.0
29	31961.0
30	38741.0
31	47354.0
32	60726.0
33	80452.0
34	114264.0
35	174047.0
36	308451.0
37	620764.0
38	959359.0
39	379755.0
40	538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.625721896697996	20.063350085826475	13.396270617982514	23.91465739949302
2	15.205061109870122	20.298656540192106	40.7860037655168	23.710278584420973
3	17.66085951318466	25.872429436159507	30.262901729890444	26.203809320765387
4	12.007377722673985	16.96726183445836	39.87851911817189	31.146841324695774
5	12.525844157336804	38.21120119939877	36.22859197071996	13.03436267254447
6	30.160962974716583	39.05037048908901	18.094917615703412	12.693748920490988
7	26.721236420617483	32.85164957610425	23.102334571465242	17.32477943181303
8	24.59465258809908	36.83464686607331	20.88766117943979	17.683039366387824
9	24.585227949414747	17.220786991670554	21.48263945763292	36.71134560128178
10-11	23.293869598203678	27.334376205401732	29.439135354959678	19.93261884143491
12-13	24.027159262256188	24.725763363288184	29.306901068526585	21.940176305929043
14-15	21.394449459593318	26.856649092179996	26.198172640831924	25.550728807394762
16-17	21.122712683357626	30.07715541231515	26.790847844432065	22.009284059895162
18-19	21.000849655108993	29.08740463730966	28.63688946405314	21.274856243528202
20-21	22.250726472592632	28.378941641618134	28.078071917873277	21.292259967915957
22-23	21.958814446381414	28.14086045322557	28.069335813449953	21.830989286943066
24-25	21.846112811127778	28.182330695728496	28.04906257667224	21.922493916471485
26-27	21.58758442112065	28.140048665423695	28.15827942530126	22.1140874881544
28-29	21.620485029284527	28.141650996861355	28.264572918937603	21.973291054916512
30-31	21.910912488350228	28.620017132889064	27.862907518631257	21.606162860129448
32-33	21.50553423786457	28.29627002567008	28.354288266221843	21.843907470243508
34-35	21.682972212083545	27.98569510629446	28.395992485751258	21.935340195870737
36-37	22.020076844002332	28.394541835428473	27.797658807906274	21.78772251266292
38-39	21.81966040879911	28.545899718763067	28.00862864275248	21.62581122968534
40-41	21.56919645315592	28.182026124542425	28.350681749581035	21.898095672720615
42-43	21.544234995515758	28.148109014174473	28.253307874496215	22.054348115813553
44-45	21.722609064412936	28.288651184927772	28.21855194310725	21.770187807552045
46-47	21.73794926392305	28.475906508730287	28.092440985351885	21.693703241994776
48-49	21.649631806974153	28.30219728302022	28.1251077381188	21.92306317188683
50-51	21.63126991162236	28.19965337158301	28.102071359360203	22.067005357434418
52-53	21.6608022856322	28.181797142161695	28.145836741697227	22.01156383050888
54-55	21.82755302187586	28.574406182121702	27.90655956691952	21.691481229082914
56-57	21.626056354887364	28.26707524359005	28.023429332950293	22.083439068572293
58-59	21.50312502693163	27.8543460712374	28.41088526310649	22.23164363872448
60-61	21.83732055583644	28.334207892223702	27.94815717036152	21.88031438157834
62-63	21.620296931650945	28.3644850938064	27.98260729948862	22.03261067505403
64-65	21.61506876599135	28.027886869347167	28.1733091941637	22.18373517049778
66-67	21.49524103943353	28.100171104570094	28.021952328520776	22.382635527475603
68-69	21.939010672621578	28.289389685388123	27.91069611436903	21.860903527621264
70-71	21.872044698913964	27.998755881079084	28.025661787962942	22.10353763204401
72-73	21.593666608046608	28.10124311759556	28.22485590476868	22.080234369589146
74-75	21.67676688367212	28.18667511835718	28.049702196003256	22.086855801967445
76-77	21.685578995896783	28.168750669228203	27.93259190428158	22.213078430593434
78-79	21.767706957359557	28.296265351199835	28.01430209546411	21.921725595976493
80-81	21.64730575287366	28.075165299898163	28.124981051824022	22.152547895404155
82-83	21.520693367188503	28.083053042002685	28.28288727676826	22.113366314040544
84-85	21.58771775445433	27.99724858603905	28.119980412689756	22.295053246816863
86-87	21.73739058898539	28.10752172105213	28.049922280644257	22.105165409318218
88-89	21.491658316473387	28.002559307646653	28.15761761007895	22.348164765801013
90-91	21.4653424905605	27.925655101000473	28.20568500106501	22.403317407374022
92-93	21.638103753825554	28.085074607576065	28.042170005933887	22.23465163266449
94-95	21.440117457452068	27.85328859645078	28.236841042967797	22.469752903129354
96-97	21.40975252965572	27.854582611592697	28.28365396275499	22.452010895996587
98-99	21.41568362395401	27.894431328698467	28.20049731334634	22.489387734001184
100	21.56714124364466	27.78172629170926	28.115263132195288	22.535869332450794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	311.0
1	298.0
2	278.5
3	285.5
4	302.0
5	315.5
6	307.0
7	287.0
8	269.0
9	243.0
10	221.0
11	219.5
12	229.0
13	244.0
14	297.0
15	352.5
16	415.0
17	538.5
18	744.5
19	1035.0
20	1616.5
21	2529.5
22	3786.5
23	5969.0
24	8834.5
25	12356.0
26	17217.0
27	23582.0
28	32059.0
29	41674.5
30	50974.5
31	61264.0
32	71980.0
33	82350.5
34	92145.0
35	100332.0
36	106565.5
37	111607.5
38	115971.0
39	118827.5
40	120225.0
41	120053.5
42	118259.5
43	115942.0
44	113037.0
45	109680.5
46	106483.0
47	103149.5
48	99909.0
49	96948.5
50	94257.5
51	90803.5
52	85546.5
53	80852.5
54	77178.0
55	72928.5
56	67416.5
57	60865.0
58	55059.0
59	49784.0
60	44122.5
61	37790.0
62	31628.0
63	25940.5
64	20906.5
65	16930.0
66	13432.5
67	9976.0
68	7373.5
69	5653.5
70	4058.0
71	2874.5
72	2119.5
73	1584.5
74	1137.5
75	768.0
76	510.0
77	327.5
78	235.0
79	161.0
80	100.5
81	59.0
82	40.0
83	43.5
84	36.0
85	16.5
86	8.5
87	7.5
88	7.5
89	8.0
90	6.5
91	3.0
92	1.5
93	2.0
94	2.5
95	2.0
96	2.5
97	2.5
98	0.5
99	0.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06000101437121585
2	0.03977738698612768
3	0.05374891505342833
4	0.07250521300679086
5	0.08647674107409152
6	0.0872742027217685
7	0.08960279073298529
8	0.10172420777767535
9	0.09716272715296304
10-11	0.1024259740276311
12-13	0.10670036845917968
14-15	0.10603050067513103
16-17	0.07571100883045231
18-19	0.09850246272106035
20-21	0.09124556172719986
22-23	0.056173198462366354
24-25	0.02118058136230054
26-27	0.17928532763073826
28-29	0.06583843363221133
30-31	0.07577480576226647
32-33	0.0737333039442134
34-35	0.020255525850995246
36-37	0.050590966928627495
38-39	0.07518468414298551
40-41	0.08065527104604958
42-43	0.07363760854649218
44-45	0.07162800519434619
46-47	0.07853402306322882
48-49	0.07499329334754304
50-51	0.07009687883080638
52-53	0.07446696866007624
54-55	0.06513666738225558
56-57	0.06768854465482192
58-59	0.06424351033685737
60-61	0.06185112539382644
62-63	0.06821486934228872
64-65	0.0638926272118795
66-67	0.06595007826288611
68-69	0.06606172289356088
70-71	0.057608629428184906
72-73	0.05665167545097254
74-75	0.058884568064468075
76-77	0.05338208269549693
78-79	0.04976160681504344
80-81	0.044657852269910786
82-83	0.05103754545132661
84-85	0.04100547792355022
86-87	0.039426503861149806
88-89	0.04105332562241084
90-91	0.03746474820786444
92-93	0.039889031616802455
94-95	0.03880448377596176
96-97	0.042074076531437375
98-99	0.04175509187236658
100	0.04976160681504344
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3134947.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.34005075782747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.66845108774552	54.445459911340244
2	12.731003756983089	17.400748859006328
3	3.7106673176471947	7.607615785002624
4	1.4637168550550659	4.001219366782031
5	0.7291281043617351	2.4914325830519752
6	0.4275698008625467	1.7532085136076376
7	0.2865119208790773	1.3706167450919153
8	0.19027190998748125	1.0402553589066599
9	0.14247092207187956	0.8762823041316058
>10	0.619181829200899	7.2417099382092305
>50	0.0245877056457276	1.1272985830973081
>100	0.006392124805157616	0.6271224089062947
>500	4.666475459740375E-5	0.01702964286618864
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.189846590707913E-5	0.0
12-13	0.0	0.0	0.0	3.189846590707913E-5	0.0
14-15	3.189846590707913E-5	0.0	0.0	6.379693181415826E-5	0.0
16-17	6.379693181415826E-5	0.0	0.0	6.379693181415826E-5	0.0
18-19	6.379693181415826E-5	0.0	0.0	6.379693181415826E-5	0.0
20-21	6.379693181415826E-5	0.0	0.0	6.379693181415826E-5	0.0
22-23	9.56953977212374E-5	3.189846590707913E-5	0.0	6.379693181415826E-5	0.0
24-25	9.56953977212374E-5	3.189846590707913E-5	0.0	9.56953977212374E-5	0.0
26-27	9.56953977212374E-5	3.189846590707913E-5	0.0	1.5949232953539567E-4	0.0
28-29	9.56953977212374E-5	3.189846590707913E-5	0.0	5.582231533738848E-4	0.0
30-31	9.56953977212374E-5	3.189846590707913E-5	0.0	0.002823014232776503	0.0
32-33	9.56953977212374E-5	3.189846590707913E-5	3.189846590707913E-5	0.009808778266426833	0.0
34-35	9.56953977212374E-5	3.189846590707913E-5	3.189846590707913E-5	0.020016287356692156	0.0
36-37	9.56953977212374E-5	3.189846590707913E-5	3.189846590707913E-5	0.037225509713561346	0.0
38-39	9.56953977212374E-5	3.189846590707913E-5	3.189846590707913E-5	0.06713032150144803	0.0
40-41	9.56953977212374E-5	3.189846590707913E-5	3.189846590707913E-5	0.10301609564691205	0.0
42-43	9.56953977212374E-5	3.189846590707913E-5	3.189846590707913E-5	0.14123045780359286	0.0
44-45	9.56953977212374E-5	3.189846590707913E-5	3.189846590707913E-5	0.18325668663616962	0.0
46-47	9.56953977212374E-5	3.189846590707913E-5	3.189846590707913E-5	0.23726078941685458	0.0
48-49	9.56953977212374E-5	3.189846590707913E-5	3.189846590707913E-5	0.29904811787886687	0.0
50-51	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	0.36651337327233924	0.0
52-53	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	0.43522266883618765	0.0
54-55	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	0.5094982467008214	0.0
56-57	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	0.5979367434281984	0.0
58-59	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	0.698576403365033	0.0
60-61	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	0.8052289241253521	0.0
62-63	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	0.9188512596863678	0.0
64-65	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	1.038422659139054	0.0
66-67	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	1.1742622762043506	0.0
68-69	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	1.3282680696037286	0.0
70-71	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	1.5012215517519116	0.0
72-73	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	1.6844622891551277	0.0
74-75	1.5949232953539565E-4	3.189846590707913E-5	3.189846590707913E-5	1.871052365478587	0.0
76-77	1.913907954424748E-4	3.189846590707913E-5	3.189846590707913E-5	2.0719489037613714	0.0
78-79	1.913907954424748E-4	3.189846590707913E-5	3.189846590707913E-5	2.3000069857640337	0.0
80-81	2.2328926134955392E-4	3.189846590707913E-5	3.189846590707913E-5	2.5497879230494167	0.0
82-83	2.2328926134955392E-4	3.189846590707913E-5	3.189846590707913E-5	2.817782884367742	0.0
84-85	2.2328926134955392E-4	3.189846590707913E-5	3.189846590707913E-5	3.087627956708678	0.0
86-87	2.3923849430309348E-4	3.189846590707913E-5	3.189846590707913E-5	3.376803499389304	0.0
88	2.5518772725663304E-4	3.189846590707913E-5	3.189846590707913E-5	3.617828307783194	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3645	0.0	35.08383	1
GGTATCA	2320	0.0	34.653233	1
TCAACGC	3535	0.0	33.7778	4
ATCAACG	3535	0.0	33.112885	3
CAACGCA	3700	0.0	32.14393	5
AACGCAG	3935	0.0	30.219933	6
GTACATG	7905	0.0	28.191101	1
TATCAAC	4400	0.0	27.030552	2
TACATGG	7990	0.0	26.770205	2
CGCAGAG	4775	0.0	24.532352	8
ACGCAGA	4800	0.0	24.479504	7
ACATGGG	8260	0.0	24.3016	3
CATGGGG	5915	0.0	21.219912	4
GCAGAGT	6135	0.0	17.948107	9
GAGTACA	5995	0.0	16.939484	1
CTGTCGC	910	0.0	16.547255	9
GTCGTAT	175	0.002324884	16.119398	1
TATACTG	2010	0.0	15.669634	5
GTGTAGG	1210	0.0	15.54212	1
CTGTCCG	400	8.743882E-9	15.293282	9
>>END_MODULE