FastQCFastQC Report
Wed 25 May 2016
SRR1033043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3134947
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43540.13888592055942253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36850.1175458468675866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35300.055.5552981
GTATCAA72150.044.1689641
TCAACGC83200.037.565314
ATCAACG83350.037.441923
CAACGCA85600.036.401115
AACGCAG90150.034.876696
TATCAAC92000.034.366882
ACGCAGA102650.030.400737
CGCAGAG104800.029.5079788
GCAGAGT119250.025.7349159
GTACATG77100.024.4575251
TACATGG78400.022.8387832
AGAGTAC109100.021.62454210-11
GAGTACT80750.021.0976212-13
ACATGGG82750.020.6742153
CAGAGTA116900.020.54349510-11
TGGTATC18450.019.1544782
AGTACTT88050.018.97484412-13
GAGTACA60600.018.8256321
TATACTG19250.018.7990075
Produced by FastQC (version 0.11.5)