##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033043_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3134947
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70077867345126	34.0	31.0	34.0	31.0	34.0
2	32.68864736788213	34.0	31.0	34.0	31.0	34.0
3	32.80067254725518	34.0	31.0	34.0	31.0	34.0
4	36.29846341899879	37.0	37.0	37.0	35.0	37.0
5	36.28350559036564	37.0	37.0	37.0	35.0	37.0
6	36.347432348936046	37.0	37.0	37.0	35.0	37.0
7	36.29234178440656	37.0	37.0	37.0	35.0	37.0
8	36.29436287120644	37.0	37.0	37.0	35.0	37.0
9	38.093042721296406	39.0	39.0	39.0	35.0	39.0
10-11	38.05743127395775	39.0	39.0	39.0	35.0	39.0
12-13	38.04262910983822	39.0	39.0	39.0	35.0	39.0
14-15	39.53978599957192	41.0	39.0	41.0	37.0	41.0
16-17	39.53464444534469	41.0	39.0	41.0	37.0	41.0
18-19	39.54658547656467	41.0	39.5	41.0	37.0	41.0
20-21	39.539837515594364	41.0	40.0	41.0	37.0	41.0
22-23	39.47413959470447	41.0	39.0	41.0	36.0	41.0
24-25	39.42453476884936	41.0	39.0	41.0	36.0	41.0
26-27	39.32044656576332	41.0	39.0	41.0	36.0	41.0
28-29	39.265421871565934	41.0	39.0	41.0	36.0	41.0
30-31	39.25945797488761	41.0	39.0	41.0	36.0	41.0
32-33	39.22340409582682	41.0	39.0	41.0	35.5	41.0
34-35	39.14784859201767	41.0	39.0	41.0	35.0	41.0
36-37	39.064196141114984	41.0	39.0	41.0	35.0	41.0
38-39	38.97120684974898	41.0	39.0	41.0	35.0	41.0
40-41	38.872719379306886	41.0	39.0	41.0	35.0	41.0
42-43	38.76168018151503	41.0	39.0	41.0	35.0	41.0
44-45	38.61013551425272	41.0	38.5	41.0	35.0	41.0
46-47	38.4282750234693	41.0	38.0	41.0	34.5	41.0
48-49	38.236263962357256	40.0	38.0	41.0	34.0	41.0
50-51	38.10708777532762	40.0	38.0	41.0	34.0	41.0
52-53	37.97538602725979	40.0	38.0	41.0	34.0	41.0
54-55	37.83203384299639	40.0	37.5	41.0	33.0	41.0
56-57	37.68999747045166	40.0	37.0	41.0	33.0	41.0
58-59	37.51575497129616	40.0	37.0	41.0	33.0	41.0
60-61	37.32759788283502	40.0	36.0	41.0	33.0	41.0
62-63	37.10048335745389	39.5	36.0	41.0	33.0	41.0
64-65	36.83532050143113	39.0	35.0	41.0	32.0	41.0
66-67	36.56397939103914	39.0	35.0	41.0	32.0	41.0
68-69	36.263544965831954	38.0	35.0	40.5	31.5	41.0
70-71	35.93863213636467	37.0	35.0	40.0	31.0	41.0
72-73	35.5934687253086	37.0	35.0	39.5	31.0	41.0
74-75	35.24790785936732	36.0	35.0	39.0	31.0	41.0
76-77	34.887432706198865	36.0	35.0	39.0	31.0	40.5
78-79	34.52390853816668	35.5	35.0	37.5	30.5	39.5
80-81	34.186039189817244	35.0	34.0	37.0	30.0	39.0
82-83	33.880604520586786	35.0	34.0	37.0	30.0	39.0
84-85	33.64871830369062	35.0	34.0	36.0	30.0	37.5
86-87	33.43136199750745	35.0	34.0	36.0	30.0	37.0
88-89	33.21458544594215	35.0	34.0	36.0	30.0	37.0
90-91	33.02712151114517	35.0	34.0	35.0	30.0	36.0
92-93	32.85235188984056	35.0	34.0	35.0	29.5	36.0
94-95	32.69212366269669	35.0	34.0	35.0	29.0	36.0
96-97	32.54783111165835	35.0	34.0	35.0	29.0	36.0
98-99	32.424356615917276	35.0	34.0	35.0	29.0	35.5
100	32.32123988061042	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16531892314466035
1101	2	0.11400282597404754
1101	3	0.16809960446506977
1101	4	0.12300919965731083
1101	5	0.09709861967407107
1101	6	0.10987457690330871
1101	7	0.1440194123622831
1101	8	0.1485784969903392
1101	9	0.24233490009073932
1101	10-11	0.24508493589097924
1101	12-13	0.20064247512849676
1101	14-15	0.2708110924456335
1101	16-17	0.3034612239708423
1101	18-19	0.33635364315983907
1101	20-21	0.2694930362914576
1101	22-23	0.27972377961771144
1101	24-25	0.3195884183358402
1101	26-27	0.35109639446497454
1101	28-29	0.2969295345073064
1101	30-31	0.39353983390299163
1101	32-33	0.3323525470000561
1101	34-35	0.26230774438750615
1101	36-37	0.28865694957775645
1101	38-39	0.25847212564265476
1101	40-41	0.2666727925694161
1101	42-43	0.3239257485437008
1101	44-45	0.3257601858811512
1101	46-47	0.35358071763091914
1101	48-49	0.3182967955464804
1101	50-51	0.35096520627546113
1101	52-53	0.31698770100273066
1101	54-55	0.33343900886588074
1101	56-57	0.30935689338413397
1101	58-59	0.29062871186054196
1101	60-61	0.30975918168886096
1101	62-63	0.299540585012565
1101	64-65	0.3139469363626475
1101	66-67	0.33045582752920666
1101	68-69	0.32575684183144915
1101	70-71	0.33373223146649167
1101	72-73	0.4069953046430186
1101	74-75	0.3591505577629164
1101	76-77	0.370399516853368
1101	78-79	0.404857193560197
1101	80-81	0.34060866750196084
1101	82-83	0.31209120599316265
1101	84-85	0.3564579608805971
1101	86-87	0.34571720836899544
1101	88-89	0.4045809781638354
1101	90-91	0.42639500433593014
1101	92-93	0.3976824094032949
1101	94-95	0.42798579150635163
1101	96-97	0.42602932349793576
1101	98-99	0.4625047162131466
1101	100	0.5088540956071697
1102	1	0.15132951843502696
1102	2	0.12851535059733266
1102	3	0.12738107837083845
1102	4	0.10064222704198755
1102	5	0.12442986555115709
1102	6	0.1291651660194404
1102	7	0.1276289306330014
1102	8	0.16422220278002442
1102	9	0.1781010949448003
1102	10-11	0.17968261649008355
1102	12-13	0.17617594441363593
1102	14-15	0.311127745537668
1102	16-17	0.2903318503196033
1102	18-19	0.27569433853255987
1102	20-21	0.297743338324139
1102	22-23	0.3144727791023527
1102	24-25	0.3052593624382709
1102	26-27	0.323711831914018
1102	28-29	0.26240978530346126
1102	30-31	0.28863682344035624
1102	32-33	0.282228251790535
1102	34-35	0.28301950257504416
1102	36-37	0.22545362901074384
1102	38-39	0.20674273596216608
1102	40-41	0.243971078010226
1102	42-43	0.21811835257342693
1102	44-45	0.20568361537401358
1102	46-47	0.23440664885173135
1102	48-49	0.21265368799955553
1102	50-51	0.21722209948205062
1102	52-53	0.28638113307351887
1102	54-55	0.2721319123594981
1102	56-57	0.24116446791387602
1102	58-59	0.2404444782060935
1102	60-61	0.2243534052667968
1102	62-63	0.200950691159548
1102	64-65	0.21888530479935753
1102	66-67	0.24949748372090852
1102	68-69	0.2914724509213613
1102	70-71	0.19233709737219584
1102	72-73	0.24902661968336304
1102	74-75	0.2789525759417373
1102	76-77	0.24048430749008531
1102	78-79	0.23444397449579668
1102	80-81	0.21492522651917767
1102	82-83	0.20767957661049508
1102	84-85	0.20872270933434578
1102	86-87	0.24394521101678635
1102	88-89	0.2462012296404339
1102	90-91	0.19650041379770755
1102	92-93	0.2456371269927473
1102	94-95	0.3179428595961937
1102	96-97	0.2719261544781446
1102	98-99	0.3289366296789282
1102	100	0.27244259774656854
1103	1	0.09018195964893039
1103	2	0.13060369507955727
1103	3	0.12035522815810396
1103	4	0.10164396911730478
1103	5	0.11924688261621696
1103	6	0.09340872882681595
1103	7	0.1260734830223882
1103	8	0.17854051420898998
1103	9	0.1491923337820893
1103	10-11	0.22709629467499326
1103	12-13	0.16886153655993041
1103	14-15	0.19967426688183565
1103	16-17	0.2202100676153762
1103	18-19	0.21725255629652906
1103	20-21	0.22324458326747276
1103	22-23	0.22560710487888969
1103	24-25	0.2311187439917859
1103	26-27	0.20656539549521824
1103	28-29	0.2651679086481167
1103	30-31	0.2609349647715078
1103	32-33	0.2183220819567282
1103	34-35	0.2827354968292468
1103	36-37	0.31588092841684556
1103	38-39	0.22649666050864425
1103	40-41	0.2803852640565836
1103	42-43	0.24664524224394313
1103	44-45	0.22958334102479228
1103	46-47	0.1879503317641067
1103	48-49	0.17888707927200898
1103	50-51	0.2711379973661252
1103	52-53	0.29616949595840225
1103	54-55	0.2412643731595523
1103	56-57	0.22408120529748743
1103	58-59	0.21900716528086406
1103	60-61	0.23087090632837004
1103	62-63	0.21372184592983956
1103	64-65	0.25807224528203676
1103	66-67	0.24214714840881157
1103	68-69	0.2048077805858668
1103	70-71	0.2070933661447043
1103	72-73	0.25291621411601284
1103	74-75	0.22369289357298072
1103	76-77	0.22114558081123903
1103	78-79	0.2181516036672022
1103	80-81	0.23221868915234012
1103	82-83	0.23739434835570705
1103	84-85	0.2512616329151811
1103	86-87	0.2275406684855099
1103	88-89	0.22888256901607917
1103	90-91	0.2188926493049621
1103	92-93	0.2772180131947266
1103	94-95	0.18856775677213022
1103	96-97	0.21915018120166962
1103	98-99	0.2692676239884122
1103	100	0.2967734528981154
1104	1	0.11310517732952974
1104	2	0.10772608350725221
1104	3	0.12066808917403193
1104	4	0.10042334419843968
1104	5	0.07472504787469347
1104	6	0.07661042705508692
1104	7	0.09596520198043379
1104	8	0.10035856980734792
1104	9	0.12567841582514916
1104	10-11	0.1466882896667272
1104	12-13	0.176959811652857
1104	14-15	0.24645484920178973
1104	16-17	0.2407485372238085
1104	18-19	0.24600246018127336
1104	20-21	0.26127696453827554
1104	22-23	0.2587393977703414
1104	24-25	0.2623492777326746
1104	26-27	0.25877084774437265
1104	28-29	0.25423302701074846
1104	30-31	0.2752710814783086
1104	32-33	0.21850302769355068
1104	34-35	0.21298865898246788
1104	36-37	0.26728699742246675
1104	38-39	0.18458612568441168
1104	40-41	0.19162371427074731
1104	42-43	0.17767534258672413
1104	44-45	0.18048066066197066
1104	46-47	0.07625069950810826
1104	48-49	0.08985850805690632
1104	50-51	0.18981857062712493
1104	52-53	0.16532876213899783
1104	54-55	0.18611849431739813
1104	56-57	0.19142528610714749
1104	58-59	0.15612894977783043
1104	60-61	0.1846818025459811
1104	62-63	0.18289484063860328
1104	64-65	0.1524851545812851
1104	66-67	0.12632779031244468
1104	68-69	0.15496209422327922
1104	70-71	0.11114838734609833
1104	72-73	0.12528464049358945
1104	74-75	0.08542069888564185
1104	76-77	0.10785868476919092
1104	78-79	0.09070175404283276
1104	80-81	0.07468616328060307
1104	82-83	0.05767530688714828
1104	84-85	0.08230501039461302
1104	86-87	0.12178866422934931
1104	88-89	0.10377746483647599
1104	90-91	0.10088583235916104
1104	92-93	0.08755030272408248
1104	94-95	0.10577975108522253
1104	96-97	0.15429845086234195
1104	98-99	0.20075199541327038
1104	100	0.1873044729900144
1105	1	0.0501698598142184
1105	2	0.09304880793417425
1105	3	0.10217346488401802
1105	4	0.052499815994998755
1105	5	0.04894714494707131
1105	6	0.08865174222924566
1105	7	0.07007097849753308
1105	8	0.0725627730729741
1105	9	0.12270386285089074
1105	10-11	0.12702316214657117
1105	12-13	0.16176300041846758
1105	14-15	0.1687276309781396
1105	16-17	0.1693233671658092
1105	18-19	0.15122545951194866
1105	20-21	0.10864933381156305
1105	22-23	0.14125389238284214
1105	24-25	0.10767989446259207
1105	26-27	0.18048536948359128
1105	28-29	0.17430026392825226
1105	30-31	0.1893031883104186
1105	32-33	0.12325981109357542
1105	34-35	0.0780376001066756
1105	36-37	0.09898555272805254
1105	38-39	0.0727537648833092
1105	40-41	0.08770289579076973
1105	42-43	0.11922925153726283
1105	44-45	0.10747376202253633
1105	46-47	0.09976855842360521
1105	48-49	0.10335255246413766
1105	50-51	0.1422512526243267
1105	52-53	0.1699681192750475
1105	54-55	0.2106131362987469
1105	56-57	0.1408864865431454
1105	58-59	0.07896758412439198
1105	60-61	0.05130800027421856
1105	62-63	0.13117998472964132
1105	64-65	0.09943661740932441
1105	66-67	0.03760115393728114
1105	68-69	0.07612327018624399
1105	70-71	0.07614386131181305
1105	72-73	0.08555868916639042
1105	74-75	0.0675583679730778
1105	76-77	0.07107124031262657
1105	78-79	0.06780466456299195
1105	80-81	0.09656695903704104
1105	82-83	0.13059045627576893
1105	84-85	0.16299343937419764
1105	86-87	0.05354152211777574
1105	88-89	0.1256776227808203
1105	90-91	0.1598655384273897
1105	92-93	0.09986438394895458
1105	94-95	0.07430666206928294
1105	96-97	0.1377372688056795
1105	98-99	0.12776644923739866
1105	100	0.16928810456984422
1106	1	-0.026756201287540193
1106	2	0.008309728932871963
1106	3	0.015935879300755573
1106	4	0.004531499425858954
1106	5	0.02058420001446848
1106	6	0.00407955817889416
1106	7	0.02370546034823917
1106	8	-0.01556406335503624
1106	9	-0.03526274508742944
1106	10-11	0.03998268346850864
1106	12-13	0.03423196262598793
1106	14-15	0.10232928004030128
1106	16-17	0.08667528349332088
1106	18-19	0.04046736428985298
1106	20-21	0.09954501583069231
1106	22-23	0.05619976706644536
1106	24-25	0.03449129990013944
1106	26-27	0.055285375645866
1106	28-29	0.07417883909820944
1106	30-31	0.06796680091618867
1106	32-33	0.1415991853238765
1106	34-35	0.17321762723936018
1106	36-37	0.13082322719746742
1106	38-39	0.08708152135766767
1106	40-41	0.12293063854006192
1106	42-43	0.00702607945946454
1106	44-45	0.07404748103959236
1106	46-47	0.08982084906264731
1106	48-49	0.14383961028588033
1106	50-51	0.16459442505691868
1106	52-53	0.12129996432557277
1106	54-55	0.1617140827681851
1106	56-57	0.11289150412897442
1106	58-59	0.1390225964349341
1106	60-61	0.13094021048623006
1106	62-63	0.15594381086758347
1106	64-65	0.17188482862223964
1106	66-67	0.14130930298590982
1106	68-69	0.11132309596192158
1106	70-71	0.12146436030671026
1106	72-73	0.16857289134316744
1106	74-75	0.1518867920272342
1106	76-77	0.14598931006398175
1106	78-79	0.11828389816270146
1106	80-81	0.18227444890897004
1106	82-83	0.1327634051951776
1106	84-85	0.15879580960929474
1106	86-87	0.09364686901184882
1106	88-89	0.1612864695670524
1106	90-91	0.1290188931792997
1106	92-93	0.195942586021161
1106	94-95	0.2057309972671746
1106	96-97	0.1632419684741322
1106	98-99	0.16181542345201194
1106	100	0.18773599553118459
1107	1	-0.02211743394838095
1107	2	0.015617705454310737
1107	3	0.03233931915258381
1107	4	0.02373894608996352
1107	5	0.05913172988842064
1107	6	0.06867585210850535
1107	7	0.07044890223418321
1107	8	0.04018569713604592
1107	9	0.07895974204770084
1107	10-11	0.12039034769976809
1107	12-13	0.05263163651836322
1107	14-15	0.08917833951618093
1107	16-17	0.10883071920774512
1107	18-19	0.14693885263262985
1107	20-21	0.11912322351115989
1107	22-23	0.13945560526930478
1107	24-25	0.12871369518919096
1107	26-27	0.12440367083133452
1107	28-29	0.14361170710957794
1107	30-31	0.11736648547892514
1107	32-33	0.08722765631219431
1107	34-35	0.11956567174851074
1107	36-37	0.12048527549202959
1107	38-39	0.09072031449476015
1107	40-41	0.13743873997216127
1107	42-43	0.15001523176201204
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2216	48-49	-0.4645949588638203
2216	50-51	-0.5987377916238259
2216	52-53	-0.5707755979995426
2216	54-55	-0.5636769603780181
2216	56-57	-0.5424393396030496
2216	58-59	-0.5346053312087662
2216	60-61	-0.5451794658841678
2216	62-63	-0.5742927151867434
2216	64-65	-0.640461355966238
2216	66-67	-0.599861132325195
2216	68-69	-0.6065509716273567
2216	70-71	-0.5934845882406776
2216	72-73	-0.6254745842674936
2216	74-75	-0.6730134347159265
2216	76-77	-0.6105572108203248
2216	78-79	-0.6431783897400507
2216	80-81	-0.6351337586500492
2216	82-83	-0.6532587393488853
2216	84-85	-0.711961636037465
2216	86-87	-0.696873980934889
2216	88-89	-0.7840998167784221
2216	90-91	-0.818000468555077
2216	92-93	-0.7927586193368228
2216	94-95	-0.6914877484742803
2216	96-97	-0.7953110679967637
2216	98-99	-0.950520987524424
2216	100	-0.9498569611536034
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22.0
3	4.0
4	19.0
5	19.0
6	82.0
7	174.0
8	396.0
9	768.0
10	1380.0
11	2157.0
12	3293.0
13	5175.0
14	5983.0
15	7717.0
16	9467.0
17	12928.0
18	15262.0
19	8084.0
20	5714.0
21	5456.0
22	5866.0
23	6248.0
24	7210.0
25	8809.0
26	11447.0
27	14896.0
28	19278.0
29	25051.0
30	32291.0
31	41344.0
32	53250.0
33	69638.0
34	96103.0
35	144241.0
36	260219.0
37	590824.0
38	1099954.0
39	562137.0
40	2041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47922886306447	20.18410399689809	13.631546278906864	23.705120861130567
2	15.490773317096911	20.559421014695285	40.27129063555482	23.678515032652985
3	17.8115052408922	25.957241567186102	30.52292170608833	25.70833148583337
4	12.097555467159275	17.156899179009805	39.603677724743555	31.141867629087365
5	12.249002453315708	38.0357397475742	36.2229464605554	13.492311338554696
6	30.08379408009131	38.93542697851032	17.85334807893084	13.127430862467532
7	26.465391599921784	32.9477659430925	23.184060208992367	17.402782247993347
8	24.786111856298046	36.266708092326056	20.792437988307885	18.154742063068014
9	24.856879558091414	16.37456709794456	21.711435631926154	37.05711771203787
10-11	23.489629420279776	26.785448843130588	29.471509056354122	20.253412680235513
12-13	24.542312836548753	24.152561430863106	29.609352247422365	21.695773485165777
14-15	21.643990258823177	26.098015607637727	26.991238586900963	25.26675554663814
16-17	20.777268340499607	29.62560214460211	27.488528091681484	22.108601423216804
18-19	20.591366053702032	28.18819124394564	30.142287159386115	21.07815554296622
20-21	21.828410787871498	27.49434479982354	29.74754114623647	20.929703266068497
22-23	21.439312114865285	27.314683971824678	29.80240144793747	21.443602465372567
24-25	21.275406662911106	27.435208583606652	29.789378054716792	21.50000669876545
26-27	21.10760528858016	27.360748287808057	29.870538336655827	21.661108086955952
28-29	21.141777148667238	27.42115273430491	29.913355529416457	21.523714587611394
30-31	21.393412450110752	27.884708626374188	29.459377902763173	21.262501020751888
32-33	21.068171168443996	27.566399049170524	29.939836303452662	21.425593478932818
34-35	21.249306607097346	27.315804701004513	29.89951664254611	21.53537204935203
36-37	21.63620310008431	27.739145191290316	29.25872431017175	21.36592739845363
38-39	21.435322511034478	27.881619689264287	29.404308908571657	21.278748891129577
40-41	21.29576435950232	27.55579443977486	29.5692109430947	21.579230257628122
42-43	21.303773237633685	27.53777017601892	29.406382308855623	21.752074277491772
44-45	21.492644054269498	27.714503626377095	29.261770613665878	21.531081705687527
46-47	21.597813296365135	28.02005903130101	28.87300806042335	21.509119611910503
48-49	21.552645068640714	27.891061635172782	28.739146148244295	21.817147147942215
50-51	21.57430412699162	27.84729693994827	28.643099867398075	21.935299065662033
52-53	21.635134501476422	27.85493662253301	28.629447323989847	21.88048155200072
54-55	21.776652045473178	28.246410545377643	28.35784783602402	21.619089573125162
56-57	21.51640841137027	27.938398958578887	28.499429176952596	22.04576345309825
58-59	21.382721940753704	27.543990376870802	28.906597145023504	22.16669053735199
60-61	21.715662178658842	27.988001073064396	28.405902874913036	21.89043387336373
62-63	21.5183223193247	27.930520037499836	28.548010540529074	22.00314710264639
64-65	21.501559675490526	27.669431094050395	28.67120241586221	22.157806814596864
66-67	21.39497095166202	27.75282963316445	28.52834513629736	22.323854278876166
68-69	21.851740502417506	27.93671654264791	28.375033991851524	21.836508963083055
70-71	21.82555239370873	27.68262110970297	28.420193387639408	22.07163310894889
72-73	21.591146880004082	27.834472111108983	28.54379988133744	22.03058112754949
74-75	21.7405194064901	27.85547809713317	28.34535933422724	22.058643162149494
76-77	21.68596470689935	27.899450931706344	28.2171756013738	22.197408760020505
78-79	21.796588586665102	28.01084037465386	28.27204096273398	21.920530075947056
80-81	21.647909837072206	27.783388363503438	28.38567924752795	22.18302255189641
82-83	21.520620221506313	27.83113066275974	28.538607302298335	22.109641813435612
84-85	21.52964627472171	27.772622631259797	28.333987783525526	22.363743310492968
86-87	21.631194644993492	27.920239507320005	28.233499540899643	22.215066306786856
88-89	21.362547007396458	27.80670322522815	28.36055235305733	22.470197414318065
90-91	21.322633067884194	27.6753255432313	28.429869064975627	22.572172323908877
92-93	21.452178692290197	27.834647716329325	28.239154226998934	22.474019364381544
94-95	21.196015800654145	27.596231307617845	28.45250146454462	22.755251427183385
96-97	21.194755375137603	27.644489867917855	28.40137763270337	22.759377124241176
98-99	21.19879634232759	27.65251506047396	28.34345233225022	22.80523626494823
100	21.28881285712326	27.621423902860236	28.227207668901578	22.862555571114918
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	20.5
2	65.0
3	125.5
4	88.5
5	62.5
6	163.5
7	372.5
8	672.5
9	842.0
10	757.5
11	684.5
12	748.5
13	925.5
14	1151.0
15	1344.5
16	1500.5
17	1737.0
18	2162.5
19	2581.0
20	3082.5
21	3931.5
22	5152.0
23	7273.5
24	10128.0
25	13334.0
26	17922.5
27	24213.0
28	32743.5
29	42147.5
30	51149.0
31	61152.0
32	71390.0
33	81545.5
34	91508.0
35	99358.0
36	105335.0
37	111290.0
38	115915.5
39	118190.5
40	119649.0
41	119713.0
42	117739.0
43	115874.5
44	113077.5
45	109459.5
46	106872.0
47	103371.5
48	99385.5
49	96259.0
50	93769.5
51	90626.0
52	85027.5
53	79846.0
54	76506.0
55	72644.5
56	67149.0
57	60428.5
58	54356.5
59	49410.0
60	43973.0
61	37471.5
62	31228.0
63	25522.0
64	20402.5
65	16287.5
66	12736.0
67	9406.5
68	6902.5
69	5176.0
70	3666.5
71	2512.5
72	1803.0
73	1311.5
74	963.5
75	652.5
76	408.5
77	258.0
78	157.5
79	83.5
80	38.5
81	21.0
82	11.0
83	6.5
84	3.0
85	0.0
86	0.0
87	0.5
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04440266454265415
2	0.2494779018592659
3	0.0025518772725663303
4	0.002360486477123856
5	1.913907954424748E-4
6	0.0
7	0.0
8	4.4657852269910784E-4
9	0.0
10-11	6.698677840486618E-4
12-13	0.0
14-15	2.3923849430309348E-4
16-17	2.0734002839601433E-4
18-19	6.060708522345035E-4
20-21	1.5949232953539565E-5
22-23	1.5949232953539565E-4
24-25	0.0013078371021902444
26-27	1.1164463067477696E-4
28-29	3.0303542611725173E-4
30-31	9.56953977212374E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	6.379693181415826E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.4354309658185608E-4
70-71	0.0
72-73	2.2328926134955392E-4
74-75	1.1164463067477696E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	7.974616476769782E-5
84-85	0.0
86-87	3.6683235793141E-4
88-89	0.002169095681681381
90-91	0.0022807403123561577
92-93	5.103754545132661E-4
94-95	4.306292897455682E-4
96-97	1.2759386362831652E-4
98-99	7.974616476769782E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3134947.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.09728639945925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.01612273581311	51.566540082444035
2	13.862131487824406	18.324985501153837
3	3.9161964702704117	7.765498790760445
4	1.5704201104495088	4.152020312314065
5	0.7927094525080385	2.6197971856991176
6	0.4660144903669328	1.848137594164872
7	0.2998825922551838	1.38749979105522
8	0.20603714614271534	1.0894797006017836
9	0.1429337132122837	0.850277752049744
>10	0.6907916765169106	7.823109371781676
>50	0.02782521107207172	1.248188139359018
>100	0.008597073317432239	0.9227751537243207
>500	1.4478867918955981E-4	0.05534730270230593
>1k	1.9305157179002743E-4	0.34634332218956476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4354	0.13888592055942253	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3685	0.1175458468675866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.189846590707913E-5	0.0
4	0.0	0.0	0.0	3.189846590707913E-5	0.0
5	0.0	0.0	0.0	3.189846590707913E-5	0.0
6	0.0	0.0	0.0	3.189846590707913E-5	0.0
7	0.0	0.0	0.0	3.189846590707913E-5	0.0
8	0.0	0.0	0.0	3.189846590707913E-5	0.0
9	0.0	0.0	0.0	3.189846590707913E-5	0.0
10-11	0.0	0.0	0.0	3.189846590707913E-5	0.0
12-13	0.0	0.0	0.0	3.189846590707913E-5	0.0
14-15	0.0	0.0	0.0	3.189846590707913E-5	0.0
16-17	0.0	0.0	0.0	3.189846590707913E-5	0.0
18-19	0.0	0.0	0.0	3.189846590707913E-5	0.0
20-21	0.0	0.0	0.0	3.189846590707913E-5	0.0
22-23	0.0	0.0	0.0	3.189846590707913E-5	0.0
24-25	1.5949232953539565E-5	0.0	0.0	7.974616476769784E-5	0.0
26-27	4.78476988606187E-5	0.0	0.0	1.5949232953539567E-4	0.0
28-29	6.379693181415826E-5	0.0	0.0	5.582231533738848E-4	0.0
30-31	6.379693181415826E-5	0.0	0.0	0.002902760397544201	0.0
32-33	6.379693181415826E-5	0.0	0.0	0.00992042289710161	0.0
34-35	6.379693181415826E-5	0.0	0.0	0.020143881220320473	0.0
36-37	6.379693181415826E-5	0.0	0.0	0.03749664667377152	0.0
38-39	6.379693181415826E-5	0.0	0.0	0.06759284925710068	0.0
40-41	6.379693181415826E-5	0.0	0.0	0.1037178618968678	0.0
42-43	6.379693181415826E-5	0.0	0.0	0.14183652865582735	0.0
44-45	6.379693181415826E-5	0.0	0.0	0.18408604674975365	0.0
46-47	6.379693181415826E-5	0.0	0.0	0.23845698188837006	0.0
48-49	6.379693181415826E-5	0.0	0.0	0.3006430411742208	0.0
50-51	6.379693181415826E-5	0.0	0.0	0.36826778889722855	0.0
52-53	6.379693181415826E-5	0.0	0.0	0.43723227218833366	0.0
54-55	6.379693181415826E-5	0.0	0.0	0.5120341747404342	0.0
56-57	6.379693181415826E-5	0.0	0.0	0.6008235545927889	0.0
58-59	6.379693181415826E-5	0.0	0.0	0.7024680162056967	0.0
60-61	6.379693181415826E-5	0.0	0.0	0.810013694011414	0.0
62-63	6.379693181415826E-5	0.0	0.0	0.9243537450553391	0.0
64-65	9.56953977212374E-5	0.0	0.0	1.045041590814773	0.0
66-67	9.56953977212374E-5	0.0	0.0	1.1820295526527242	0.0
68-69	9.56953977212374E-5	0.0	0.0	1.3379971017053878	0.0
70-71	9.56953977212374E-5	0.0	0.0	1.5128166441091349	0.0
72-73	9.56953977212374E-5	0.0	0.0	1.6980510356315435	0.0
74-75	9.56953977212374E-5	0.0	0.0	1.8862679337162638	0.0
76-77	9.56953977212374E-5	0.0	0.0	2.089285719981869	0.0
78-79	9.56953977212374E-5	0.0	0.0	2.319465049967352	0.0
80-81	9.56953977212374E-5	0.0	0.0	2.5719254583889297	0.0
82-83	9.56953977212374E-5	0.0	0.0	2.8428710278036595	0.0
84-85	1.1164463067477696E-4	0.0	0.0	3.1157943021046286	0.0
86-87	1.2759386362831652E-4	0.0	0.0	3.408989051489547	0.0
88	1.2759386362831652E-4	0.0	0.0	3.653044214144609	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3530	0.0	55.555298	1
GTATCAA	7215	0.0	44.168964	1
TCAACGC	8320	0.0	37.56531	4
ATCAACG	8335	0.0	37.44192	3
CAACGCA	8560	0.0	36.40111	5
AACGCAG	9015	0.0	34.87669	6
TATCAAC	9200	0.0	34.36688	2
ACGCAGA	10265	0.0	30.40073	7
CGCAGAG	10480	0.0	29.507978	8
GCAGAGT	11925	0.0	25.734915	9
GTACATG	7710	0.0	24.457525	1
TACATGG	7840	0.0	22.838783	2
AGAGTAC	10910	0.0	21.624542	10-11
GAGTACT	8075	0.0	21.09762	12-13
ACATGGG	8275	0.0	20.674215	3
CAGAGTA	11690	0.0	20.543495	10-11
TGGTATC	1845	0.0	19.154478	2
AGTACTT	8805	0.0	18.974844	12-13
GAGTACA	6060	0.0	18.825632	1
TATACTG	1925	0.0	18.799007	5
>>END_MODULE