FastQCFastQC Report
Wed 25 May 2016
SRR1033042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4141828
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55950.033.6124341
TCAACGC55000.032.6519624
GGTATCA37250.032.4374661
ATCAACG56600.031.645493
CAACGCA57750.031.016075
GTACATG123400.029.5274931
AACGCAG62350.028.6447756
TACATGG124600.027.995562
ACATGGG133250.025.6490043
TATCAAC71800.025.5354462
ACGCAGA73200.024.0778927
CGCAGAG74000.023.5846338
CATGGGG94100.021.082954
ACCGTCG1857.132039E-620.342548
GAGTACA89050.019.4819551
CTGTCGC10550.019.1768159
GCAGAGT90700.018.5191659
GTATTAG18600.018.4522781
AGTACAT89150.017.61282
GTCGTAC3452.3817847E-816.353181
Produced by FastQC (version 0.11.5)