##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033042_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4141828 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.421747836945425 33.0 31.0 34.0 30.0 34.0 2 31.547618346295405 33.0 31.0 34.0 30.0 34.0 3 31.65375940285304 34.0 31.0 34.0 30.0 34.0 4 35.05868664753824 37.0 35.0 37.0 33.0 37.0 5 35.061257493068275 37.0 35.0 37.0 33.0 37.0 6 35.17402147071293 37.0 35.0 37.0 33.0 37.0 7 35.09408164704087 37.0 35.0 37.0 33.0 37.0 8 35.09030626090702 37.0 35.0 37.0 33.0 37.0 9 36.719626696231714 39.0 38.0 39.0 33.0 39.0 10-11 36.70251529035006 39.0 37.0 39.0 33.0 39.0 12-13 36.628299267859504 39.0 37.0 39.0 33.0 39.0 14-15 37.981105564982414 40.0 38.0 41.0 33.0 41.0 16-17 37.966480742319575 40.0 38.0 41.0 33.0 41.0 18-19 37.9632026003977 40.0 38.0 41.0 33.0 41.0 20-21 37.969100962183845 40.0 38.0 41.0 33.0 41.0 22-23 37.91840861088389 40.0 38.0 41.0 33.0 41.0 24-25 37.910040928787964 40.0 38.0 41.0 33.0 41.0 26-27 37.792722802588614 40.0 38.0 41.0 32.0 41.0 28-29 37.79129142977449 40.0 38.0 41.0 32.5 41.0 30-31 37.92992417840625 40.0 38.0 41.0 33.0 41.0 32-33 37.91169949597135 40.0 38.0 41.0 33.0 41.0 34-35 37.861939703918175 40.0 38.0 41.0 33.0 41.0 36-37 37.777545083958096 40.0 38.0 41.0 32.0 41.0 38-39 37.68637507400114 40.0 38.0 41.0 32.0 41.0 40-41 37.60923027223728 40.0 38.0 41.0 32.0 41.0 42-43 37.48024459248428 40.0 38.0 41.0 31.5 41.0 44-45 37.335134993534254 40.0 38.0 41.0 31.0 41.0 46-47 37.191375160919286 40.0 37.0 41.0 31.0 41.0 48-49 37.06150496833765 40.0 37.0 41.0 31.0 41.0 50-51 36.921066736716256 40.0 37.0 41.0 30.0 41.0 52-53 36.74862415822193 40.0 36.0 41.0 30.0 41.0 54-55 36.57623481709042 40.0 36.0 41.0 30.0 41.0 56-57 36.386947260967865 39.5 35.5 41.0 30.0 41.0 58-59 36.16925159615513 39.0 35.0 41.0 30.0 41.0 60-61 35.92960064976141 39.0 35.0 41.0 29.0 41.0 62-63 35.66344027323201 38.5 35.0 40.5 29.0 41.0 64-65 35.37429717023498 38.0 35.0 40.0 28.5 41.0 66-67 35.06254581310475 37.5 34.5 40.0 28.0 41.0 68-69 34.737382503570885 37.0 34.0 40.0 28.0 41.0 70-71 34.39887713830705 36.5 34.0 39.0 28.0 41.0 72-73 34.042966052670465 36.0 34.0 39.0 27.5 41.0 74-75 33.686376401917215 35.5 34.0 39.0 27.0 40.0 76-77 33.32144949041824 35.0 33.5 37.5 26.0 39.5 78-79 32.93849998116774 35.0 33.0 37.0 26.0 39.0 80-81 32.59956871700129 35.0 33.0 36.5 26.0 39.0 82-83 32.385647110406325 35.0 33.0 36.0 26.0 38.0 84-85 32.17566434434264 35.0 33.0 36.0 26.0 37.0 86-87 32.01411031554183 35.0 33.0 35.5 26.0 37.0 88-89 31.805309153349683 35.0 33.0 35.0 25.0 36.5 90-91 31.600650365007915 35.0 33.0 35.0 25.0 36.0 92-93 31.383408485335462 35.0 33.0 35.0 24.5 36.0 94-95 31.18166290343298 35.0 33.0 35.0 24.0 36.0 96-97 31.003662996145664 35.0 33.0 35.0 23.0 35.0 98-99 30.820802553848203 35.0 32.0 35.0 21.5 35.0 100 30.666992448744853 35.0 32.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.018129802425423946 1101 2 0.12552345106415075 1101 3 0.11855856787735419 1101 4 0.01738612141119944 1101 5 0.00569015702200204 1101 6 0.08756790343819887 1101 7 0.12494483398448608 1101 8 0.17956515617659363 1101 9 0.2102346872607015 1101 10-11 0.18243157560472412 1101 12-13 0.17581945435888002 1101 14-15 0.2758686215607682 1101 16-17 0.2813924794940945 1101 18-19 0.2565840829155448 1101 20-21 0.3024863897594514 1101 22-23 0.32876602080901307 1101 24-25 0.3907738744032869 1101 26-27 0.31038632635578267 1101 28-29 0.37137338327853797 1101 30-31 0.357951070222569 1101 32-33 0.3588132396574153 1101 34-35 0.3975962927639216 1101 36-37 0.33730311492973897 1101 38-39 0.38898279668512004 1101 40-41 0.3254147761300388 1101 42-43 0.3661009981503014 1101 44-45 0.3503282325460475 1101 46-47 0.34952050588705674 1101 48-49 0.33169029018650775 1101 50-51 0.3267965638171759 1101 52-53 0.28394056958293845 1101 54-55 0.2510537868894076 1101 56-57 0.3609446035981989 1101 58-59 0.392475776773864 1101 60-61 0.3590420172783908 1101 62-63 0.3126941081175971 1101 64-65 0.2883827835153383 1101 66-67 0.2602919737097906 1101 68-69 0.32443861260335893 1101 70-71 0.2690080349842958 1101 72-73 0.3013096858190636 1101 74-75 0.26056850459391967 1101 76-77 0.25976742706331635 1101 78-79 0.21250265858295592 1101 80-81 0.22190344996791111 1101 82-83 0.26968000453231866 1101 84-85 0.27478517237683064 1101 86-87 0.24307732969603535 1101 88-89 0.28741606729424163 1101 90-91 0.34039927671968684 1101 92-93 0.3141444854790265 1101 94-95 0.33035553415175656 1101 96-97 0.352661890594991 1101 98-99 0.31239670861662105 1101 100 0.26627848033785284 1102 1 0.01107231876159176 1102 2 0.06484370471364542 1102 3 0.08801558859632763 1102 4 -0.07113599175767149 1102 5 -0.010221019430638023 1102 6 0.06317121860283947 1102 7 0.08627361284984403 1102 8 0.20061139650990611 1102 9 0.1662111532897086 1102 10-11 0.16309744314997943 1102 12-13 0.0868270998290086 1102 14-15 0.15898844825073155 1102 16-17 0.1530928955021622 1102 18-19 0.11946223282384949 1102 20-21 0.26895868126260325 1102 22-23 0.20021557759694986 1102 24-25 0.19542236713829908 1102 26-27 0.1408907369765302 1102 28-29 0.1323437836710184 1102 30-31 0.19177490177793288 1102 32-33 0.18928414079412192 1102 34-35 0.19611903345876414 1102 36-37 0.25643334175417465 1102 38-39 0.2447239639882426 1102 40-41 0.1595966132707929 1102 42-43 0.23421578178887614 1102 44-45 0.23678442807508304 1102 46-47 0.20121127519493598 1102 48-49 0.12538830345538798 1102 50-51 0.20836050905014503 1102 52-53 0.21599494249166185 1102 54-55 0.19788268447407376 1102 56-57 0.20747098192810398 1102 58-59 0.18027343337418955 1102 60-61 0.15660672266435682 1102 62-63 0.159591828639563 1102 64-65 0.1901741618996553 1102 66-67 0.19076243141272187 1102 68-69 0.1997823409649655 1102 70-71 0.11094450422753255 1102 72-73 0.18366789820583307 1102 74-75 0.18376997416032737 1102 76-77 0.16722877811465509 1102 78-79 0.12329455000671885 1102 80-81 0.15284104163608703 1102 82-83 0.14281183819950627 1102 84-85 0.11009586760976475 1102 86-87 0.19722614311429254 1102 88-89 0.18939400700149278 1102 90-91 0.14126820199612666 1102 92-93 0.13105654234927044 1102 94-95 0.2699601397086795 1102 96-97 0.3057034633175242 1102 98-99 0.31431472765551405 1102 100 0.28534703963361707 1103 1 0.1198304676059685 1103 2 0.16660995273707968 1103 3 0.1663411458144104 1103 4 0.17499145308703135 1103 5 0.041924216124598956 1103 6 0.1773203990580967 1103 7 0.25235874552227244 1103 8 0.2518581586947519 1103 9 0.3885390538687332 1103 10-11 0.3243511163740038 1103 12-13 0.315800392327688 1103 14-15 0.4345265657609829 1103 16-17 0.43432745284159324 1103 18-19 0.405186238340427 1103 20-21 0.41374930605805815 1103 22-23 0.38292079297218606 1103 24-25 0.3602558667882505 1103 26-27 0.3456774497907986 1103 28-29 0.3368121216952744 1103 30-31 0.37096593698690583 1103 32-33 0.30725027804189153 1103 34-35 0.33966166161346933 1103 36-37 0.4164171521999265 1103 38-39 0.4338809339559404 1103 40-41 0.4592779131203173 1103 42-43 0.39030005129912126 1103 44-45 0.40574134537266104 1103 46-47 0.3804159468354058 1103 48-49 0.36370776686325 1103 50-51 0.34794684957548583 1103 52-53 0.35135910889708555 1103 54-55 0.3543212619674776 1103 56-57 0.32914484097506147 1103 58-59 0.31458453233850747 1103 60-61 0.3332351135424716 1103 62-63 0.3380248217989532 1103 64-65 0.3506545776873722 1103 66-67 0.3525230449583603 1103 68-69 0.3301844731095471 1103 70-71 0.4069513838789902 1103 72-73 0.3516350976598446 1103 74-75 0.3461833182267213 1103 76-77 0.34036801287448526 1103 78-79 0.40693342553031187 1103 80-81 0.36860063201873317 1103 82-83 0.3476833361246605 1103 84-85 0.3804737202076893 1103 86-87 0.3189113419305869 1103 88-89 0.2701045043168868 1103 90-91 0.3086842025350762 1103 92-93 0.2971132059805157 1103 94-95 0.2554321726795621 1103 96-97 0.26489120281632594 1103 98-99 0.21221709042325188 1103 100 0.18489802304520708 1104 1 -0.0038600640309454093 1104 2 0.08554768216811581 1104 3 0.06543172648177631 1104 4 0.07008328334695335 1104 5 -0.009171574050625964 1104 6 0.09142118456060189 1104 7 0.1718267594205969 1104 8 0.09101162089856984 1104 9 0.14639057656788168 1104 10-11 0.16587725584915347 1104 12-13 0.2079765769241675 1104 14-15 0.2311170393983204 1104 16-17 0.1319985125148193 1104 18-19 0.2135115568492978 1104 20-21 0.16895710414708986 1104 22-23 0.16816389907074836 1104 24-25 0.13196391453367795 1104 26-27 0.027349545990738022 1104 28-29 0.06351468176021768 1104 30-31 0.07213593716074485 1104 32-33 0.08458774386420487 1104 34-35 0.13933130110891057 1104 36-37 0.1445718559521083 1104 38-39 0.17923041510056947 1104 40-41 0.2786317988105509 1104 42-43 0.2532713031684537 1104 44-45 0.30383699114396023 1104 46-47 0.171705187652492 1104 48-49 0.18540301167791284 1104 50-51 0.1479726633208145 1104 52-53 0.16707005689272592 1104 54-55 0.19085995265321998 1104 56-57 0.20779774730059586 1104 58-59 0.25965400906393654 1104 60-61 0.26376846595291426 1104 62-63 0.32856143888895417 1104 64-65 0.25135618251116654 1104 66-67 0.25022487143449723 1104 68-69 0.3303590955044413 1104 70-71 0.33946886668090315 1104 72-73 0.3195929878730297 1104 74-75 0.2587120653179724 1104 76-77 0.2662884839427946 1104 78-79 0.23507468010424049 1104 80-81 0.25704129530786446 1104 82-83 0.2708538810745793 1104 84-85 0.290020072857736 1104 86-87 0.22733479063310824 1104 88-89 0.19876307622927314 1104 90-91 0.23621328751282178 1104 92-93 0.24473692689632998 1104 94-95 0.3488056534971129 1104 96-97 0.33227881653893476 1104 98-99 0.3514834516091909 1104 100 0.39854557063847196 1105 1 0.04565766796738302 1105 2 0.10298201040055588 1105 3 0.13947608804138767 1105 4 0.122691238416202 1105 5 0.06861562114382735 1105 6 0.21836152247502127 1105 7 0.1374240472045969 1105 8 0.2330823714216521 1105 9 0.3521463731129799 1105 10-11 0.30378469957662446 1105 12-13 0.3184761595918246 1105 14-15 0.33280374795874224 1105 16-17 0.23144658348525127 1105 18-19 0.2915971295323203 1105 20-21 0.3429827820978062 1105 22-23 0.25951044823959535 1105 24-25 0.23741827200230148 1105 26-27 0.26520505873625666 1105 28-29 0.31158791867748903 1105 30-31 0.3406384398249287 1105 32-33 0.2613591455095161 1105 34-35 0.2508317711843233 1105 36-37 0.28453629343003684 1105 38-39 0.2770606971927876 1105 40-41 0.27903590294551606 1105 42-43 0.28453107754243945 1105 44-45 0.2903253294651549 1105 46-47 0.20188835549502215 1105 48-49 0.22655430397642107 1105 50-51 0.24919389451036267 1105 52-53 0.3150945913601788 1105 54-55 0.294896868498995 1105 56-57 0.3375215977323549 1105 58-59 0.26918721934143264 1105 60-61 0.24554833959478373 1105 62-63 0.23906217035946042 1105 64-65 0.2568446871541141 1105 66-67 0.25776189528471605 1105 68-69 0.2859433780479108 1105 70-71 0.2714159505580511 1105 72-73 0.2600570017935766 1105 74-75 0.24705764434261823 1105 76-77 0.2659555999253129 1105 78-79 0.2805804445196358 1105 80-81 0.3597220720276155 1105 82-83 0.3582311324805687 1105 84-85 0.38177439044648764 1105 86-87 0.25856336906768007 1105 88-89 0.25726737510045794 1105 90-91 0.35336905086147397 1105 92-93 0.2951460138929036 1105 94-95 0.24278983985806235 1105 96-97 0.3335259184290855 1105 98-99 0.3549169573440665 1105 100 0.3783818452938057 1106 1 0.004591596863200209 1106 2 0.05055222539181514 1106 3 0.07413709717249262 1106 4 0.10334248721851225 1106 5 0.08302056577245054 1106 6 0.07359457720303908 1106 7 0.09856833934542664 1106 8 0.1413384614992097 1106 9 0.16391991143445495 1106 10-11 0.13849563920102526 1106 12-13 0.1392443595467654 1106 14-15 0.19907209720011565 1106 16-17 0.25733059849007844 1106 18-19 0.2434466559072348 1106 20-21 0.19077581453468895 1106 22-23 0.14196881231719516 1106 24-25 0.17662105938430983 1106 26-27 0.10103645249227355 1106 28-29 0.14519532885054787 1106 30-31 0.13515603082718286 1106 32-33 0.13764119675711584 1106 34-35 0.14709856550427247 1106 36-37 0.13853764783429767 1106 38-39 0.1857309986718505 1106 40-41 0.21365571525564775 1106 42-43 0.1530246730016458 1106 44-45 0.10508474406034907 1106 46-47 0.07868548117925656 1106 48-49 0.1609681456139569 1106 50-51 0.137915451443412 1106 52-53 0.12417714581345507 1106 54-55 0.10563450892883708 1106 56-57 0.11357426851943586 1106 58-59 0.1357194995692268 1106 60-61 0.19942189654415898 1106 62-63 0.11034621576423831 1106 64-65 0.09627441569352158 1106 66-67 0.05880784589915322 1106 68-69 0.10274628659988849 1106 70-71 0.12711498471759342 1106 72-73 0.12690050808657816 1106 74-75 0.18948795482238978 1106 76-77 0.10715383666376255 1106 78-79 0.08875809273376234 1106 80-81 0.1664623313233946 1106 82-83 0.13630297135460978 1106 84-85 0.10402348918039905 1106 86-87 0.10380724914358552 1106 88-89 0.12592088192377204 1106 90-91 0.15173391720243856 1106 92-93 0.18919546017915678 1106 94-95 0.20766875075542401 1106 96-97 0.18349526326611354 1106 98-99 0.15073984416475383 1106 100 0.16200896868971526 1107 1 0.12169034878352036 1107 2 0.13637399045685683 1107 3 0.17576820907144963 1107 4 0.19311899340705452 1107 5 0.12539396613357923 1107 6 0.1284289284011635 1107 7 0.1706019419232021 1107 8 0.11069298343674916 1107 9 0.20289720915133813 1107 10-11 0.22320058529136588 1107 12-13 0.2555405329978555 1107 14-15 0.29492921632569846 1107 16-17 0.26865703436666166 1107 18-19 0.24824435440626047 1107 20-21 0.2844384151616879 1107 22-23 0.2747083221406825 1107 24-25 0.20656719616234653 1107 26-27 0.19958094430948847 1107 28-29 0.202716849191944 1107 30-31 0.2235619059555063 1107 32-33 0.21745964637555204 1107 34-35 0.2249727466133109 1107 36-37 0.2656837122814153 1107 38-39 0.3033085090611465 1107 40-41 0.27034683461108955 1107 42-43 0.3052498039016882 1107 44-45 0.2395036423655199 1107 46-47 0.2252168809693842 1107 48-49 0.2593847327292593 1107 50-51 0.301605978142355 1107 52-53 0.2506435241148566 1107 54-55 0.24675854471070835 1107 56-57 0.2965251009800767 1107 58-59 0.28073826787314715 1107 60-61 0.3348561322438499 1107 62-63 0.29680132410170756 1107 64-65 0.23855050976024472 1107 66-67 0.2837493060263512 1107 68-69 0.3491090618382984 1107 70-71 0.31750450478591574 1107 72-73 0.31916384348753724 1107 74-75 0.26952207665203787 1107 76-77 0.2956132475556146 1107 78-79 0.21446180076663524 1107 80-81 0.2864142969632084 1107 82-83 0.25721993433468526 1107 84-85 0.3092884507546714 1107 86-87 0.2767613381235847 1107 88-89 0.2612493233623532 1107 90-91 0.29906631796198724 1107 92-93 0.3396599613575617 1107 94-95 0.2733715921811495 1107 96-97 0.27825210172141013 1107 98-99 0.20034081431695583 1107 100 0.19592076187216279 1108 1 -0.03522108147667424 1108 2 0.03379524132958167 1108 3 0.04146760987756082 1108 4 0.03265405941417754 1108 5 -0.013416732086362515 1108 6 0.04761013706897188 1108 7 0.07708665517727553 1108 8 0.08332497841789177 1108 9 0.06824798335846083 1108 10-11 0.13887018882720525 1108 12-13 0.16401600290359397 1108 14-15 0.14605651305432588 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2214 94-95 0.045242405565463883 2214 96-97 0.004026285470946078 2214 98-99 -0.030765928542848542 2214 100 0.021592342789674035 2215 1 -0.2779374956629539 2215 2 -0.33841658153349385 2215 3 -0.39634629082580375 2215 4 -0.316055232255529 2215 5 -0.24450384480238796 2215 6 -0.32242949354120043 2215 7 -0.2863519422797083 2215 8 -0.32811595907922 2215 9 -0.40764012559897367 2215 10-11 -0.3756608778963795 2215 12-13 -0.47222777683380457 2215 14-15 -0.5189254082803245 2215 16-17 -0.5410681051477084 2215 18-19 -0.42671884183663167 2215 20-21 -0.3998695704534967 2215 22-23 -0.4601978136159488 2215 24-25 -0.4377840294065365 2215 26-27 -0.4083806291317842 2215 28-29 -0.41017889683597275 2215 30-31 -0.45101822792474877 2215 32-33 -0.5015696343727285 2215 34-35 -0.4569940092587288 2215 36-37 -0.41866777023467705 2215 38-39 -0.47495005170305404 2215 40-41 -0.5375275919357989 2215 42-43 -0.4601217681416898 2215 44-45 -0.4430611777920177 2215 46-47 -0.46783162327822225 2215 48-49 -0.38483054045703824 2215 50-51 -0.34011563478190254 2215 52-53 -0.34671618400139437 2215 54-55 -0.4236537688781752 2215 56-57 -0.5100131793856164 2215 58-59 -0.49859514734400534 2215 60-61 -0.5448479076014223 2215 62-63 -0.6064681160597658 2215 64-65 -0.7157083457691513 2215 66-67 -0.6143787694140173 2215 68-69 -0.6046495534702885 2215 70-71 -0.6011723445207693 2215 72-73 -0.7084764664983751 2215 74-75 -0.62867514903391 2215 76-77 -0.5684350629057917 2215 78-79 -0.6464246108366822 2215 80-81 -0.6453559470478893 2215 82-83 -0.6117840540777024 2215 84-85 -0.6047059597539146 2215 86-87 -0.5647668423755015 2215 88-89 -0.5867078749423591 2215 90-91 -0.6255366091218768 2215 92-93 -0.6151774184702248 2215 94-95 -0.719725558255675 2215 96-97 -0.7178643646288414 2215 98-99 -0.5735997092723579 2215 100 -0.5392140817273585 2216 1 -0.5244384687067623 2216 2 -0.6317189671347272 2216 3 -0.4938080843534749 2216 4 -0.573064501232075 2216 5 -0.44445269388108954 2216 6 -0.6269683322947373 2216 7 -0.5542091738553694 2216 8 -0.48393371333677493 2216 9 -0.5528998284925564 2216 10-11 -0.517688496106075 2216 12-13 -0.6729758727570214 2216 14-15 -0.7856391508181062 2216 16-17 -0.802210645836837 2216 18-19 -0.7818441955677926 2216 20-21 -0.6411608630776797 2216 22-23 -0.6519596019269756 2216 24-25 -0.7294465771856338 2216 26-27 -0.796195273784484 2216 28-29 -0.6929883295889852 2216 30-31 -0.824712220917192 2216 32-33 -0.918231314536925 2216 34-35 -1.1104147718713975 2216 36-37 -1.0736823688142891 2216 38-39 -0.9832980322024341 2216 40-41 -0.9330057602608406 2216 42-43 -0.9952688294149539 2216 44-45 -0.8876731723725442 2216 46-47 -0.8880743245729121 2216 48-49 -0.9172243308769268 2216 50-51 -0.84782453927982 2216 52-53 -0.8997328609113922 2216 54-55 -0.8678955110839581 2216 56-57 -0.9436737186088209 2216 58-59 -0.8927331155016986 2216 60-61 -0.8860197397001457 2216 62-63 -0.9037306887677232 2216 64-65 -0.9542690533863762 2216 66-67 -0.9169114450696796 2216 68-69 -0.903486856351833 2216 70-71 -0.890634146012232 2216 72-73 -0.9086492972127118 2216 74-75 -0.9388520051160612 2216 76-77 -0.7604127868658779 2216 78-79 -0.8644498053504606 2216 80-81 -0.7790218307788734 2216 82-83 -1.032722555122639 2216 84-85 -0.8591976842786266 2216 86-87 -0.8824829431453765 2216 88-89 -0.9861514086651475 2216 90-91 -0.9904010679479072 2216 92-93 -1.2264739434515448 2216 94-95 -1.1356094810264743 2216 96-97 -1.05488381922164 2216 98-99 -0.9877106381660781 2216 100 -1.0462112954232126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54038.0 3 10901.0 4 6146.0 5 5008.0 6 4547.0 7 5039.0 8 5865.0 9 6438.0 10 7296.0 11 7920.0 12 8625.0 13 9462.0 14 9897.0 15 10332.0 16 10310.0 17 10386.0 18 10177.0 19 10221.0 20 10398.0 21 10760.0 22 12307.0 23 16948.0 24 24333.0 25 30163.0 26 30636.0 27 30153.0 28 34586.0 29 42239.0 30 51975.0 31 64265.0 32 82786.0 33 109872.0 34 156847.0 35 240896.0 36 440524.0 37 868655.0 38 1245378.0 39 445054.0 40 445.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.388331620902946 19.181193334881083 13.176976721656903 24.253498322559075 2 15.635330211716353 19.93489626734413 40.088866087848736 24.340907433090784 3 18.30336533897255 24.795178644805528 29.861341106096916 27.040114910125006 4 12.303274480075867 16.36923522980556 38.7627406329091 32.564749657209475 5 13.062066109092157 38.00155185208544 35.60850507734975 13.32787696147265 6 31.39038549647097 37.68650404712333 17.74398247760888 13.179127978796823 7 27.92468998208861 32.335286219825875 22.250799149764582 17.48922464832094 8 25.318622273759694 36.33611091105162 20.284228421005313 18.061038394183377 9 25.310832087717156 16.81077952412427 20.651353542725744 37.227034845432826 10-11 24.08177953766601 26.6044153467807 28.810010255855 20.503794859698285 12-13 24.666647492155167 24.014891344569115 28.694841758315594 22.623619404960124 14-15 22.05942990947407 26.067282225602785 25.599655143909985 26.27363272101316 16-17 21.877741227739012 29.357667935441214 26.20844765371745 22.556143183102325 18-19 21.81471270784659 28.360035211799456 27.92376657501207 21.901485505341885 20-21 22.99950194016177 27.635554560993242 27.40700526841462 21.95793823043037 22-23 22.718688575756563 27.364769581443362 27.427639500862362 22.488902341937713 24-25 22.554591153171096 27.441931395684676 27.397847457741726 22.605629993402502 26-27 22.305586761155435 27.434001516815893 27.543959179729384 22.716452542299294 28-29 22.26949967372599 27.537296827688913 27.552952181832108 22.640251316752995 30-31 22.536360182210167 27.869505047265065 27.22348169773675 22.37065307278802 32-33 22.284938020447633 27.56969603985634 27.711341152707327 22.434024786988704 34-35 22.491444697281985 27.344899141487357 27.623092881693523 22.540563279537135 36-37 22.78551233949382 27.581662606806844 27.17969058747025 22.453134466229084 38-39 22.508529870918125 27.750960388200035 27.34665380913917 22.39385593174267 40-41 22.303752276646964 27.517698192878438 27.63137498905095 22.547174541423647 42-43 22.350354425715572 27.391771041289765 27.59023736103099 22.667637171963673 44-45 22.33769453183459 27.665035697139807 27.49204065770008 22.505229113325523 46-47 22.43045393654592 27.740233782250414 27.451702914449307 22.37760936675436 48-49 22.460919685064486 27.53059332684738 27.316274225518544 22.692212762569593 50-51 22.357978888354527 27.488785971056807 27.383431904197746 22.76980323639092 52-53 22.36260566007465 27.520498972635178 27.48899182940525 22.627903537884926 54-55 22.496762298403926 27.813405950944457 27.314218260407486 22.375613490244135 56-57 22.286424603073936 27.59890988029703 27.385899233992795 22.728766282636244 58-59 22.25502433421705 27.228520579382554 27.732032126070923 22.78442296032947 60-61 22.619173734891703 27.574925883142086 27.22452343612313 22.581376945843086 62-63 22.30520441843853 27.613320065953552 27.347034234454508 22.734441281153405 64-65 22.358972413814765 27.406667138584684 27.451724840226355 22.782635607374193 66-67 22.18789875511394 27.38604369332264 27.381622392655714 23.044435158907707 68-69 22.506045456225678 27.53159594970741 27.334908731670655 22.627449862396258 70-71 22.446658692089645 27.32033073839486 27.427085224456803 22.805925345058693 72-73 22.26266236754584 27.359835881618118 27.48996183310299 22.88753991773305 74-75 22.382142351543404 27.412004470258434 27.36229869462849 22.843554483569676 76-77 22.33309232653852 27.434637676923185 27.293645078400488 22.93862491813781 78-79 22.370109286363657 27.546726886513234 27.344321917385482 22.738841909737626 80-81 22.334629889653016 27.360220834065025 27.445028805966075 22.86012047031588 82-83 22.216081123061024 27.374407141024705 27.609521591734254 22.79999014418002 84-85 22.23346861206533 27.234200479506022 27.46037891893276 23.07195198949589 86-87 22.39861475224132 27.31815000152167 27.40010260378851 22.8831326424485 88-89 22.15606315393857 27.303926517823456 27.442051000626556 23.09795932761142 90-91 22.12891892564261 27.141368623862117 27.569034899007576 23.160677551487694 92-93 22.239323058098037 27.240508402053692 27.494110416570805 23.026058123277462 94-95 22.11051410624765 26.975986331109976 27.67909169704057 23.23440786560181 96-97 22.043074189579716 27.037688436445638 27.640803417414045 23.2784339565606 98-99 22.097453589474362 27.036303553834806 27.603323910771405 23.262918945919424 100 22.17303743226892 26.921574590726856 27.52463513810244 23.38075283890179 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 344.0 1 323.5 2 294.5 3 307.0 4 346.0 5 368.0 6 392.0 7 363.5 8 291.0 9 273.5 10 276.5 11 267.5 12 242.5 13 264.0 14 295.0 15 326.0 16 412.0 17 538.0 18 672.0 19 877.5 20 1278.0 21 1924.5 22 2875.5 23 4626.0 24 7094.0 25 10286.5 26 14982.0 27 21558.5 28 30884.5 29 41992.5 30 52664.5 31 63909.0 32 75861.0 33 88248.5 34 101141.0 35 113068.0 36 122702.0 37 132598.5 38 140677.5 39 145902.0 40 150646.5 41 153023.0 42 152398.5 43 152821.0 44 152720.5 45 149853.0 46 148023.0 47 144244.0 48 140313.0 49 139072.0 50 137195.5 51 133484.5 52 126677.0 53 120964.0 54 117609.5 55 112459.0 56 103038.0 57 91188.0 58 82650.5 59 77108.5 60 70104.5 61 61040.5 62 52924.5 63 44836.0 64 36330.0 65 28729.5 66 22197.0 67 16294.0 68 12003.0 69 9188.0 70 6430.0 71 4387.5 72 3217.5 73 2178.0 74 1517.5 75 1129.0 76 773.0 77 526.5 78 345.5 79 198.5 80 120.0 81 64.0 82 44.0 83 38.0 84 30.5 85 22.5 86 14.5 87 10.5 88 8.0 89 5.5 90 4.0 91 5.0 92 4.5 93 3.5 94 3.0 95 2.5 96 3.0 97 1.5 98 1.0 99 2.5 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06004595072513875 2 0.04022378524651434 3 0.05420311997504484 4 0.07250421794434728 5 0.0856385151676989 6 0.08643526481543898 7 0.08884965768737861 8 0.10068018275988283 9 0.09616526808935572 10-11 0.10119927722734987 12-13 0.10601599100686943 14-15 0.10744048280131382 16-17 0.07382006205955438 18-19 0.0968292261291391 20-21 0.09104675520084368 22-23 0.05621913802311443 24-25 0.020860354413558456 26-27 0.181308832718307 28-29 0.0644401457520689 30-31 0.07727264386642806 32-33 0.07153846079557143 34-35 0.020015316908379585 36-37 0.05099197745536512 38-39 0.07573950439274639 40-41 0.08065279388714355 42-43 0.07461681170729446 44-45 0.07197305151252055 46-47 0.07909551048474249 48-49 0.07579986421454489 50-51 0.07068135132603284 52-53 0.07445987617061839 54-55 0.06527311129288807 56-57 0.06839975006204989 58-59 0.06512824772057169 60-61 0.06247241556143809 62-63 0.06891884452951692 64-65 0.06497131218389561 66-67 0.06698733023196521 68-69 0.06685453862400853 70-71 0.05851281125145709 72-73 0.05739011856600516 74-75 0.059635503936909015 76-77 0.05429969568992241 78-79 0.05088332977612784 80-81 0.04555959349350094 82-83 0.051716295316947006 84-85 0.041865572399433296 86-87 0.039354603812616074 88-89 0.041575845254800535 90-91 0.0378335363032941 92-93 0.04054972828422619 94-95 0.03919766827594 96-97 0.04197422007867058 98-99 0.042614034189734584 100 0.050967833526645724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4141828.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.93583136081729 #Duplication Level Percentage of deduplicated Percentage of total 1 73.22526298496311 40.22690698693429 2 14.489794986489935 15.920178676612537 3 5.164503533554799 8.511488855451342 4 2.446260141412713 5.375493383733514 5 1.3175395519949666 3.619006531980112 6 0.7917449962112815 2.6097101755580328 7 0.5232982856576809 2.0123478460606603 8 0.373129373168166 1.6398537856107065 9 0.25284753812158517 1.2501350742820703 >10 1.303703809635307 12.720681812052137 >50 0.0790155003307716 2.9633810571899852 >100 0.03215128940481553 2.8759311733042936 >500 7.480090548550269E-4 0.27488464123031137 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.4143928719396362E-5 0.0 2 0.0 0.0 0.0 2.4143928719396362E-5 0.0 3 0.0 0.0 0.0 2.4143928719396362E-5 0.0 4 0.0 0.0 0.0 2.4143928719396362E-5 0.0 5 0.0 0.0 0.0 4.8287857438792724E-5 0.0 6 0.0 0.0 0.0 4.8287857438792724E-5 0.0 7 0.0 0.0 0.0 4.8287857438792724E-5 0.0 8 0.0 0.0 0.0 4.8287857438792724E-5 0.0 9 0.0 0.0 0.0 4.8287857438792724E-5 0.0 10-11 0.0 0.0 0.0 6.035982179849091E-5 0.0 12-13 0.0 0.0 0.0 7.243178615818908E-5 0.0 14-15 0.0 0.0 0.0 9.657571487758545E-5 0.0 16-17 0.0 0.0 0.0 1.0864767923728362E-4 0.0 18-19 0.0 0.0 0.0 1.6900750103577455E-4 0.0 20-21 0.0 0.0 0.0 1.6900750103577455E-4 0.0 22-23 0.0 0.0 0.0 1.810794653954727E-4 0.0 24-25 0.0 0.0 0.0 2.052233941148691E-4 0.0 26-27 0.0 0.0 0.0 3.5008696643124725E-4 0.0 28-29 0.0 0.0 0.0 0.0011106207210922327 0.0 30-31 0.0 0.0 0.0 0.004635634314124102 0.0 32-33 0.0 0.0 0.0 0.013218800973869509 0.0 34-35 0.0 0.0 0.0 0.0258822915871929 0.0 36-37 0.0 2.4143928719396362E-5 0.0 0.04965198941143863 0.0 38-39 0.0 4.8287857438792724E-5 0.0 0.0863628330292808 0.0 40-41 0.0 4.8287857438792724E-5 0.0 0.12846984471590805 0.0 42-43 0.0 4.8287857438792724E-5 0.0 0.17382421481529411 0.0 44-45 0.0 4.8287857438792724E-5 0.0 0.22942768265606395 0.0 46-47 0.0 4.8287857438792724E-5 0.0 0.2961735736008352 0.0 48-49 0.0 4.8287857438792724E-5 0.0 0.3718285742430637 0.0 50-51 0.0 4.8287857438792724E-5 0.0 0.45050156597521673 0.0 52-53 0.0 4.8287857438792724E-5 0.0 0.5318545337952227 0.0 54-55 0.0 4.8287857438792724E-5 0.0 0.6233720956060946 0.0 56-57 0.0 4.8287857438792724E-5 0.0 0.7335408423527003 0.0 58-59 0.0 4.8287857438792724E-5 0.0 0.8578941472219512 0.0 60-61 0.0 4.8287857438792724E-5 0.0 0.9893336951703451 0.0 62-63 0.0 4.8287857438792724E-5 0.0 1.1239360977809798 0.0 64-65 0.0 4.8287857438792724E-5 0.0 1.2709967676108231 0.0 66-67 0.0 6.035982179849091E-5 0.0 1.4414891202628404 0.0 68-69 0.0 7.243178615818908E-5 0.0 1.636813503602757 0.0 70-71 0.0 7.243178615818908E-5 0.0 1.8419523939671083 0.0 72-73 0.0 7.243178615818908E-5 0.0 2.060382517091487 0.0 74-75 0.0 7.243178615818908E-5 0.0 2.2894238968880405 0.0 76-77 0.0 7.243178615818908E-5 0.0 2.539313559133793 0.0 78-79 0.0 7.243178615818908E-5 0.0 2.8204454651424444 0.0 80-81 0.0 7.243178615818908E-5 0.0 3.129161809713006 0.0 82-83 0.0 7.243178615818908E-5 0.0 3.4503847093602147 0.0 84-85 0.0 7.243178615818908E-5 0.0 3.777631519222913 0.0 86-87 0.0 7.243178615818908E-5 0.0 4.1283220838721455 0.0 88 0.0 7.243178615818908E-5 0.0 4.413944760622604 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5595 0.0 33.612434 1 TCAACGC 5500 0.0 32.651962 4 GGTATCA 3725 0.0 32.437466 1 ATCAACG 5660 0.0 31.64549 3 CAACGCA 5775 0.0 31.01607 5 GTACATG 12340 0.0 29.527493 1 AACGCAG 6235 0.0 28.644775 6 TACATGG 12460 0.0 27.99556 2 ACATGGG 13325 0.0 25.649004 3 TATCAAC 7180 0.0 25.535446 2 ACGCAGA 7320 0.0 24.077892 7 CGCAGAG 7400 0.0 23.584633 8 CATGGGG 9410 0.0 21.08295 4 ACCGTCG 185 7.132039E-6 20.34254 8 GAGTACA 8905 0.0 19.481955 1 CTGTCGC 1055 0.0 19.176815 9 GCAGAGT 9070 0.0 18.519165 9 GTATTAG 1860 0.0 18.452278 1 AGTACAT 8915 0.0 17.6128 2 GTCGTAC 345 2.3817847E-8 16.35318 1 >>END_MODULE