FastQCFastQC Report
Wed 25 May 2016
SRR1033042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4141828
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45100.1088891185244776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53900.046.0743331
GTATCAA96250.042.8395161
TCAACGC115000.035.637844
ATCAACG115450.035.4577943
CAACGCA118550.034.5297625
AACGCAG123700.033.168176
TATCAAC126350.032.5471732
ACGCAGA145650.028.0082657
CGCAGAG148150.027.4087418
GTACATG122100.027.015911
TACATGG123500.025.7079372
GTCGTAC2850.024.8018231
GCAGAGT164000.024.3585859
ACATGGG129750.023.6896063
GTGGTAT29500.022.8429011
GAGTACA92300.020.8303091
AGAGTAC148750.020.63173910-11
CATGGGG89750.020.5278914
GAGTACT104400.019.82998712-13
CAGAGTA159450.019.58619510-11
Produced by FastQC (version 0.11.5)