##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033042_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4141828 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70123312701541 34.0 31.0 34.0 31.0 34.0 2 32.69122522712194 34.0 31.0 34.0 31.0 34.0 3 32.80242250523199 34.0 31.0 34.0 31.0 34.0 4 36.2957293253124 37.0 37.0 37.0 35.0 37.0 5 36.278555507375 37.0 37.0 37.0 35.0 37.0 6 36.34439672531066 37.0 37.0 37.0 35.0 37.0 7 36.28958566121046 37.0 37.0 37.0 35.0 37.0 8 36.29136168860706 37.0 37.0 37.0 35.0 37.0 9 38.092718480825376 39.0 39.0 39.0 35.0 39.0 10-11 38.055284405822746 39.0 39.0 39.0 35.0 39.0 12-13 38.040003351177305 39.0 39.0 39.0 35.0 39.0 14-15 39.538186158382246 41.0 39.0 41.0 37.0 41.0 16-17 39.53001150699643 41.0 39.0 41.0 37.0 41.0 18-19 39.5379456848522 41.0 39.5 41.0 37.0 41.0 20-21 39.52873043013858 41.0 39.5 41.0 37.0 41.0 22-23 39.4627520746878 41.0 39.0 41.0 36.0 41.0 24-25 39.415291750405856 41.0 39.0 41.0 36.0 41.0 26-27 39.312030340226585 41.0 39.0 41.0 36.0 41.0 28-29 39.25995961203604 41.0 39.0 41.0 36.0 41.0 30-31 39.26337489147304 41.0 39.0 41.0 36.0 41.0 32-33 39.237654847086844 41.0 39.0 41.0 35.5 41.0 34-35 39.16563616354904 41.0 39.0 41.0 35.0 41.0 36-37 39.08272506728913 41.0 39.0 41.0 35.0 41.0 38-39 38.995258373838794 41.0 39.0 41.0 35.0 41.0 40-41 38.898880880616 41.0 39.0 41.0 35.0 41.0 42-43 38.78805735052252 41.0 39.0 41.0 35.0 41.0 44-45 38.63497494343078 41.0 38.0 41.0 35.0 41.0 46-47 38.458027952874914 41.0 38.0 41.0 34.5 41.0 48-49 38.27408260314044 40.0 38.0 41.0 34.0 41.0 50-51 38.133794788194976 40.0 38.0 41.0 34.0 41.0 52-53 37.992065580704946 40.0 38.0 41.0 34.0 41.0 54-55 37.837070974458626 40.0 37.0 41.0 33.5 41.0 56-57 37.678010047737374 40.0 37.0 41.0 33.0 41.0 58-59 37.48703048509016 40.0 36.5 41.0 33.0 41.0 60-61 37.27962291046369 39.5 36.0 41.0 33.0 41.0 62-63 37.036454917973415 39.0 35.5 41.0 33.0 41.0 64-65 36.760205759389336 39.0 35.0 41.0 32.0 41.0 66-67 36.47203046577502 38.5 35.0 41.0 32.0 41.0 68-69 36.16027102042866 37.5 35.0 40.0 32.0 41.0 70-71 35.829418435531366 37.0 35.0 40.0 31.0 41.0 72-73 35.48304589181395 36.5 35.0 39.0 31.0 41.0 74-75 35.136929515180256 36.0 35.0 39.0 31.0 41.0 76-77 34.78657105027056 35.5 35.0 38.5 31.0 40.0 78-79 34.432928286737166 35.0 34.5 37.0 30.5 39.0 80-81 34.11538262815356 35.0 34.0 37.0 30.0 39.0 82-83 33.82852511016874 35.0 34.0 36.5 30.0 39.0 84-85 33.62263160131227 35.0 34.0 36.0 30.0 37.0 86-87 33.43125016297152 35.0 34.0 36.0 30.0 37.0 88-89 33.23502641828681 35.0 34.0 35.0 30.0 36.5 90-91 33.06231427282833 35.0 34.0 35.0 30.0 36.0 92-93 32.900217247070614 35.0 34.0 35.0 30.0 36.0 94-95 32.75508736722046 35.0 34.0 35.0 29.5 36.0 96-97 32.616842611523225 35.0 34.0 35.0 29.0 36.0 98-99 32.49701206810133 35.0 34.0 35.0 29.0 35.0 100 32.39659758927701 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1523690556193813 1101 2 0.13820915871498585 1101 3 0.17285435561086615 1101 4 0.15272819400928483 1101 5 0.13141170958608228 1101 6 0.11686675422419768 1101 7 0.15010354768572398 1101 8 0.14277911287543787 1101 9 0.19328689737179872 1101 10-11 0.25047399025290673 1101 12-13 0.25499285000006466 1101 14-15 0.3522944780908972 1101 16-17 0.33108745367236025 1101 18-19 0.32091684340025495 1101 20-21 0.32146292741465743 1101 22-23 0.33715474906626497 1101 24-25 0.35043421931643337 1101 26-27 0.34842466446755793 1101 28-29 0.36329937392326883 1101 30-31 0.33051682350694733 1101 32-33 0.2977181199808072 1101 34-35 0.271493764077114 1101 36-37 0.24634551282826322 1101 38-39 0.19391787947094485 1101 40-41 0.1959501530069403 1101 42-43 0.20794914584641333 1101 44-45 0.1626862565242746 1101 46-47 0.11087780431819283 1101 48-49 0.16231565307952422 1101 50-51 0.18297800923333085 1101 52-53 0.1678616224437448 1101 54-55 0.14712871585848575 1101 56-57 0.14485607744800433 1101 58-59 0.14446000038698514 1101 60-61 0.11070157051881324 1101 62-63 0.0936831146069963 1101 64-65 0.09678217963787006 1101 66-67 0.13669644361529265 1101 68-69 0.12613711768938174 1101 70-71 0.07936029020049062 1101 72-73 0.07199530649646846 1101 74-75 0.06159501300457748 1101 76-77 0.06742143319790728 1101 78-79 0.06759831909007374 1101 80-81 0.06277157938646383 1101 82-83 0.06271773265343938 1101 84-85 0.11993279535018075 1101 86-87 0.15760440362868167 1101 88-89 0.16160297652147904 1101 90-91 0.1819350707553724 1101 92-93 0.19145886092050546 1101 94-95 0.17988502578235455 1101 96-97 0.18450008437690002 1101 98-99 0.1996618307142981 1101 100 0.21957974463725094 1102 1 0.1309722674505167 1102 2 0.08800180639489241 1102 3 0.13713868776533644 1102 4 0.0958146785106706 1102 5 0.12209466209714748 1102 6 0.13295382607377348 1102 7 0.17653858336131378 1102 8 0.16280800863262357 1102 9 0.23464630138605713 1102 10-11 0.2240835499056999 1102 12-13 0.23010872394823423 1102 14-15 0.291243641499328 1102 16-17 0.28556801938098175 1102 18-19 0.26527887187058496 1102 20-21 0.26720376617367236 1102 22-23 0.29872558566319896 1102 24-25 0.3337906548775038 1102 26-27 0.34618251885547124 1102 28-29 0.3711335889507623 1102 30-31 0.3318492244199902 1102 32-33 0.33891508278195914 1102 34-35 0.34498271646213396 1102 36-37 0.32198249872558904 1102 38-39 0.3797479901829206 1102 40-41 0.38597897687410665 1102 42-43 0.3523460141021033 1102 44-45 0.3864297701295598 1102 46-47 0.3692739372800702 1102 48-49 0.3549894082366265 1102 50-51 0.3418309696845512 1102 52-53 0.3787790828904605 1102 54-55 0.352857984355353 1102 56-57 0.3779194268000481 1102 58-59 0.36232338059127756 1102 60-61 0.4009929206174334 1102 62-63 0.3698681141024238 1102 64-65 0.4132609311648636 1102 66-67 0.3819337297307186 1102 68-69 0.35290248129231117 1102 70-71 0.33510633527513534 1102 72-73 0.2787316735618077 1102 74-75 0.33321125050126454 1102 76-77 0.22720449598960357 1102 78-79 0.2611998586383848 1102 80-81 0.2851706992107381 1102 82-83 0.2627533618852951 1102 84-85 0.31223196654558194 1102 86-87 0.33912613531012425 1102 88-89 0.3299214675889104 1102 90-91 0.326811636933229 1102 92-93 0.3918755240598557 1102 94-95 0.35199867167737153 1102 96-97 0.36367936035167503 1102 98-99 0.3072608679523867 1102 100 0.30895368736684503 1103 1 0.1480090029443204 1103 2 0.14952871542831048 1103 3 0.12958582721521594 1103 4 0.07768138253636891 1103 5 0.12131025628000458 1103 6 0.12915141480818448 1103 7 0.14743355814970727 1103 8 0.13994378002593777 1103 9 0.17839396405385344 1103 10-11 0.1887682067097387 1103 12-13 0.22170150507977837 1103 14-15 0.27056511754878443 1103 16-17 0.29618553632939637 1103 18-19 0.3065619481117352 1103 20-21 0.29803492437655876 1103 22-23 0.30788481784311017 1103 24-25 0.25341365204457844 1103 26-27 0.2627100939209157 1103 28-29 0.3138442608124734 1103 30-31 0.3048103817386689 1103 32-33 0.23692390540958996 1103 34-35 0.2828143209830358 1103 36-37 0.30250158838995844 1103 38-39 0.28315004538931277 1103 40-41 0.2996427099376646 1103 42-43 0.3413019878543153 1103 44-45 0.4098249104690197 1103 46-47 0.37949518431402396 1103 48-49 0.2928946494875859 1103 50-51 0.28742495732927154 1103 52-53 0.2800721802341073 1103 54-55 0.3500853868266347 1103 56-57 0.3343861958212955 1103 58-59 0.33648841949864305 1103 60-61 0.3501113755609424 1103 62-63 0.33438085617539315 1103 64-65 0.3042035463817072 1103 66-67 0.32467583715556003 1103 68-69 0.2859630572875247 1103 70-71 0.3150668575667268 1103 72-73 0.33762033980205786 1103 74-75 0.2877231294120435 1103 76-77 0.2814157705337408 1103 78-79 0.3082363606382046 1103 80-81 0.2982036348770194 1103 82-83 0.29452950086703567 1103 84-85 0.28857151388113067 1103 86-87 0.2855491222953219 1103 88-89 0.325166324309734 1103 90-91 0.28376692513178625 1103 92-93 0.36743774695321463 1103 94-95 0.3754098886518449 1103 96-97 0.36204341919674476 1103 98-99 0.3827445928474802 1103 100 0.382204960961694 1104 1 0.09222513172068147 1104 2 0.11791691005057459 1104 3 0.14781199448955107 1104 4 0.08423315694665234 1104 5 0.0714054595299558 1104 6 0.08767317296646837 1104 7 0.10931303139866344 1104 8 0.1045102267545488 1104 9 0.14944385469040355 1104 10-11 0.1476755591953136 1104 12-13 0.14949710311915254 1104 14-15 0.21707447582986106 1104 16-17 0.210880658613128 1104 18-19 0.2167982791566203 1104 20-21 0.2124153286587429 1104 22-23 0.22679107848679791 1104 24-25 0.22508710370718177 1104 26-27 0.20003586884018176 1104 28-29 0.25545174913656155 1104 30-31 0.19215634097012213 1104 32-33 0.19425462495056678 1104 34-35 0.20349618046087414 1104 36-37 0.20559191369165575 1104 38-39 0.20369821147907885 1104 40-41 0.18649690346163084 1104 42-43 0.16826035345859935 1104 44-45 0.18083353466418117 1104 46-47 0.10049171057566753 1104 48-49 0.07754099349389065 1104 50-51 0.15334029577660147 1104 52-53 0.18811655734025834 1104 54-55 0.10676878331898365 1104 56-57 0.1275878621817057 1104 58-59 0.1397063342378999 1104 60-61 0.1696365093002754 1104 62-63 0.13747819830866348 1104 64-65 0.17874045884746437 1104 66-67 0.14120417522789097 1104 68-69 0.17894177881357365 1104 70-71 0.15244335010841326 1104 72-73 0.13139252669056134 1104 74-75 0.13437493027780079 1104 76-77 0.11097759455926592 1104 78-79 0.0982491045179188 1104 80-81 0.10260502608363709 1104 82-83 0.04551662505642895 1104 84-85 0.12451137870061757 1104 86-87 0.11182382133907964 1104 88-89 0.11646949936726259 1104 90-91 0.1676210842193555 1104 92-93 0.13583635986812226 1104 94-95 0.19033797335901426 1104 96-97 0.23255669545482505 1104 98-99 0.22482584233398484 1104 100 0.24583499359131622 1105 1 0.07142413204715581 1105 2 0.08239756712505653 1105 3 0.11266547487266365 1105 4 0.0590842780395846 1105 5 0.06079326588752565 1105 6 0.0738784275906994 1105 7 0.1097185078720031 1105 8 0.07760571677527395 1105 9 0.08355610653696033 1105 10-11 0.10476795672931871 1105 12-13 0.12880359856929857 1105 14-15 0.19464170923360768 1105 16-17 0.22085150335502846 1105 18-19 0.12167349851147691 1105 20-21 0.1606747480356674 1105 22-23 0.1657734196892804 1105 24-25 0.12818080148227295 1105 26-27 0.18510124788937787 1105 28-29 0.18207881473926335 1105 30-31 0.20010807981905288 1105 32-33 0.17474909855805976 1105 34-35 0.18332253749555605 1105 36-37 0.12199360115288016 1105 38-39 0.1832876632958147 1105 40-41 0.14066066015787015 1105 42-43 0.18306770063571065 1105 44-45 0.1459545502919184 1105 46-47 0.1951657983200974 1105 48-49 0.11711418154460063 1105 50-51 0.21026960292002883 1105 52-53 0.2417853158278902 1105 54-55 0.2680182739267849 1105 56-57 0.2297070554006666 1105 58-59 0.2276308657003625 1105 60-61 0.2816954220608068 1105 62-63 0.22307910623641192 1105 64-65 0.31783481964591687 1105 66-67 0.31850525704365396 1105 68-69 0.30787854366818124 1105 70-71 0.3073476007864784 1105 72-73 0.2598761510718788 1105 74-75 0.2232901202753581 1105 76-77 0.2234014502936219 1105 78-79 0.2870061417134977 1105 80-81 0.2598739714130147 1105 82-83 0.2839644503641523 1105 84-85 0.2508869215594771 1105 86-87 0.24070949842169398 1105 88-89 0.17074986804129821 1105 90-91 0.22271011389349837 1105 92-93 0.26861913839664453 1105 94-95 0.23709795601718753 1105 96-97 0.26552878028129356 1105 98-99 0.2997671189844482 1105 100 0.2745519805768524 1106 1 0.05449856523289753 1106 2 0.03675476696196256 1106 3 0.04644758267019711 1106 4 0.04614818074298199 1106 5 0.07081603468677145 1106 6 0.08788830182528073 1106 7 0.08557886324837938 1106 8 0.07743238655751128 1106 9 0.08203481956063285 1106 10-11 0.10119777601725133 1106 12-13 0.09768550529625486 1106 14-15 0.2037770740622804 1106 16-17 0.18590356985043144 1106 18-19 0.15374318864959235 1106 20-21 0.11489773247146928 1106 22-23 0.1676949484897392 1106 24-25 0.12781783425162985 1106 26-27 0.14136769941738692 1106 28-29 0.12613194684477236 1106 30-31 0.13965353824766424 1106 32-33 0.1330370553907798 1106 34-35 0.081687884712359 1106 36-37 0.09380429693830905 1106 38-39 0.06172851124271972 1106 40-41 0.0819559021789118 1106 42-43 0.07150441251311435 1106 44-45 0.016281088625810014 1106 46-47 0.09294962158173803 1106 48-49 0.19844375810037462 1106 50-51 0.19105123892195053 1106 52-53 0.14032999749426978 1106 54-55 0.16196235736212827 1106 56-57 0.1348465999268953 1106 58-59 0.11833872191665762 1106 60-61 0.17296782382284448 1106 62-63 0.13144516643895088 1106 64-65 0.17824239388630048 1106 66-67 0.14126135472388057 1106 68-69 0.14865217241580808 1106 70-71 0.14535760386354468 1106 72-73 0.1668736493892098 1106 74-75 0.16794211741718357 1106 76-77 0.19158571095346844 1106 78-79 0.11778455128033016 1106 80-81 0.1481496570465879 1106 82-83 0.151643471707132 1106 84-85 0.16314138676026602 1106 86-87 0.14623151385774946 1106 88-89 0.1928832395724669 1106 90-91 0.1891745852554152 1106 92-93 0.21604185345600513 1106 94-95 0.271330888941236 1106 96-97 0.22451393991568835 1106 98-99 0.2720118790899946 1106 100 0.2607343760832066 1107 1 0.027924410618226148 1107 2 0.11684621309903065 1107 3 0.06325770046898072 1107 4 0.058719734671122126 1107 5 0.08272931321250354 1107 6 0.0901685269060053 1107 7 0.07909806151005228 1107 8 0.06732842397723715 1107 9 0.11803097341255864 1107 10-11 0.10163368755604552 1107 12-13 0.12581022772307904 1107 14-15 0.13692298854515883 1107 16-17 0.08832261236961614 1107 18-19 0.11138309526262447 1107 20-21 0.11203448127337623 1107 22-23 0.13139340848609038 1107 24-25 0.12781852122734705 1107 26-27 0.13919532952492375 1107 28-29 0.17370474839714944 1107 30-31 0.15673021534812648 1107 32-33 0.19109995076598096 1107 34-35 0.17747292227428346 1107 36-37 0.2517020153621772 1107 38-39 0.25283920315729347 1107 40-41 0.23333297427261357 1107 42-43 0.24509432296684963 1107 44-45 0.24311758400236982 1107 46-47 0.2562113609345431 1107 48-49 0.24519846062093364 1107 50-51 0.21428016526107996 1107 52-53 0.2754449675883066 1107 54-55 0.30481432791000174 1107 56-57 0.2781285155022104 1107 58-59 0.29462513929476586 1107 60-61 0.30797879342572543 1107 62-63 0.30460924099779874 1107 64-65 0.3280290535760102 1107 66-67 0.26562880592614135 1107 68-69 0.269436828960643 1107 70-71 0.30727657283816967 1107 72-73 0.2698916380520302 1107 74-75 0.26870610057264344 1107 76-77 0.3162987491089382 1107 78-79 0.2909397842072181 1107 80-81 0.277750578347181 1107 82-83 0.261496792467625 1107 84-85 0.28517557581177044 1107 86-87 0.2841130115126589 1107 88-89 0.26234923222605744 1107 90-91 0.2850966363084453 1107 92-93 0.31550961519928933 1107 94-95 0.3445346147209918 1107 96-97 0.3596532849260612 1107 98-99 0.3615896839120012 1107 100 0.3382408597982334 1108 1 -0.07025618044762894 1108 2 -0.018180563472363076 1108 3 0.0011546823942438778 1108 4 -0.006065135280792333 1108 5 4.392908057795353E-4 1108 6 0.007934109161446656 1108 7 -0.01807632905065759 1108 8 -0.008943149778104953 1108 9 -0.0223397770133289 1108 10-11 -0.02909115384628791 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-0.043250252465163896 2214 72-73 -0.04988330300463417 2214 74-75 -0.035404360436473326 2214 76-77 -0.019466229478581454 2214 78-79 -0.031475414332170715 2214 80-81 -0.035158977089565724 2214 82-83 0.007604234703833868 2214 84-85 -0.0067432095658759295 2214 86-87 -0.04981160408426888 2214 88-89 -0.011351786083835691 2214 90-91 -1.4009739678044753E-4 2214 92-93 -0.024969045575019777 2214 94-95 -0.11488641255010634 2214 96-97 -0.06016576083380443 2214 98-99 -0.039852125910073255 2214 100 -0.06539703655065665 2215 1 -0.2796575574554865 2215 2 -0.2089505813376391 2215 3 -0.24876823373842427 2215 4 -0.1523189740123314 2215 5 -0.1991419005012247 2215 6 -0.2533665209520706 2215 7 -0.23439137532186294 2215 8 -0.21335160564079558 2215 9 -0.2782505163524718 2215 10-11 -0.2509554910728866 2215 12-13 -0.24894445441056234 2215 14-15 -0.40780841752273744 2215 16-17 -0.4164190463470945 2215 18-19 -0.4220472056446951 2215 20-21 -0.42739571284155176 2215 22-23 -0.4724907416714643 2215 24-25 -0.47252302616212205 2215 26-27 -0.46721521994137305 2215 28-29 -0.5456865755216924 2215 30-31 -0.503840327036194 2215 32-33 -0.3973048884726822 2215 34-35 -0.4097327575748366 2215 36-37 -0.4068016742818443 2215 38-39 -0.43786570255042534 2215 40-41 -0.4340738120755958 2215 42-43 -0.45294739793646954 2215 44-45 -0.4789833537577124 2215 46-47 -0.46555960033579424 2215 48-49 -0.4158256804797489 2215 50-51 -0.4099840569894013 2215 52-53 -0.40599848074089806 2215 54-55 -0.4613283650543991 2215 56-57 -0.46848870310133606 2215 58-59 -0.44706872664090014 2215 60-61 -0.401554756980417 2215 62-63 -0.37458602268309704 2215 64-65 -0.4157208555253007 2215 66-67 -0.3774181642928198 2215 68-69 -0.4095766580163698 2215 70-71 -0.34956231817849925 2215 72-73 -0.34590514955753804 2215 74-75 -0.39533790467827146 2215 76-77 -0.35326216307186087 2215 78-79 -0.3303726793854693 2215 80-81 -0.375301196288099 2215 82-83 -0.38387424664819747 2215 84-85 -0.3596553052376663 2215 86-87 -0.3994261806018642 2215 88-89 -0.33439166135896414 2215 90-91 -0.30797316021522647 2215 92-93 -0.2878101510317066 2215 94-95 -0.2852979142455183 2215 96-97 -0.349069206025284 2215 98-99 -0.37618920233852293 2215 100 -0.374470436612782 2216 1 -0.3095996599607318 2216 2 -0.2684670272246237 2216 3 -0.2890199209662825 2216 4 -0.2374859886017049 2216 5 -0.25721547541361645 2216 6 -0.23721357728631887 2216 7 -0.23428704234711972 2216 8 -0.234568753539655 2216 9 -0.35363476198561017 2216 10-11 -0.3277573920518364 2216 12-13 -0.3845331280140627 2216 14-15 -0.46582949716736977 2216 16-17 -0.43684114848411326 2216 18-19 -0.44078295666822953 2216 20-21 -0.48156463713350917 2216 22-23 -0.5199282894724746 2216 24-25 -0.5016040071814132 2216 26-27 -0.51171185461844 2216 28-29 -0.5207241350272938 2216 30-31 -0.5012514372413293 2216 32-33 -0.5067956419993251 2216 34-35 -0.4757199119060971 2216 36-37 -0.4425236080540813 2216 38-39 -0.5093258903877711 2216 40-41 -0.48257154875144437 2216 42-43 -0.4673177582973054 2216 44-45 -0.5144852147277348 2216 46-47 -0.44196375376722585 2216 48-49 -0.4672137620301342 2216 50-51 -0.5491612497426743 2216 52-53 -0.559786427111419 2216 54-55 -0.49547650933024556 2216 56-57 -0.49481713475753963 2216 58-59 -0.44469746206095095 2216 60-61 -0.5108744168490773 2216 62-63 -0.5670421403654373 2216 64-65 -0.6379914175965524 2216 66-67 -0.5486866238500596 2216 68-69 -0.5563087216583114 2216 70-71 -0.5330106113991633 2216 72-73 -0.5578716887743198 2216 74-75 -0.6110766599508111 2216 76-77 -0.5977826178876455 2216 78-79 -0.7107338453663701 2216 80-81 -0.6369080388069719 2216 82-83 -0.6057097866093386 2216 84-85 -0.7197972631087666 2216 86-87 -0.7165686691975708 2216 88-89 -0.8336999256995625 2216 90-91 -0.8503158358796625 2216 92-93 -0.822736838120754 2216 94-95 -0.7450155956453415 2216 96-97 -0.9315690171592195 2216 98-99 -0.9724520160059704 2216 100 -0.9987507911520126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28.0 3 11.0 4 10.0 5 32.0 6 84.0 7 199.0 8 494.0 9 851.0 10 1545.0 11 2455.0 12 3758.0 13 5811.0 14 6901.0 15 9186.0 16 10821.0 17 15568.0 18 17371.0 19 9479.0 20 7202.0 21 6789.0 22 7440.0 23 7988.0 24 9641.0 25 11874.0 26 15244.0 27 20034.0 28 26162.0 29 34109.0 30 43752.0 31 56161.0 32 71918.0 33 94386.0 34 130642.0 35 199960.0 36 376552.0 37 842424.0 38 1443452.0 39 649784.0 40 1710.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.061189934036406 19.349054869660975 13.369336458284772 24.220418738017845 2 15.822002053353302 20.122305699870612 39.735460133627704 24.320232113148382 3 18.23411316326691 24.967024529693617 30.190561156429162 26.608301150610313 4 12.424494258207545 16.5713041240175 38.4965924932963 32.50760912447866 5 12.797381631632115 37.7513390261663 35.79915186961468 13.652127472586903 6 31.249284735766302 37.80255375286166 17.491705349283144 13.456456162088895 7 27.71575739021514 32.436136894144326 22.230836239457556 17.61726947618298 8 25.58973513315533 35.76630739600518 20.17889732936052 18.46506014147897 9 25.564509197388208 16.061193270217885 20.833240781606577 37.541056750787334 10-11 24.270326747835032 26.16887212753384 28.818455249858005 20.74234587477312 12-13 25.088040836075283 23.56075626510806 28.938997466819 22.41220543199766 14-15 22.267696102386004 25.44754523136711 26.314966671624234 25.96979199462265 16-17 21.615716464879466 28.924009771544984 26.82120332549298 22.639070438082566 18-19 21.429636353298324 27.61699388081318 29.231650859763313 21.721718906125187 20-21 22.578402890994372 26.84479254628543 28.918816633478823 21.65798792924138 22-23 22.229765872277664 26.656804658724347 28.942562817485694 22.1708666515123 24-25 22.101568334217415 26.776752910521868 28.888898814834096 22.23277994042662 26-27 21.82541359203423 26.842998843745857 29.026578393137513 22.305009171082396 28-29 21.804681375348064 26.89459722628981 29.04523546066859 22.25548593769353 30-31 22.089087089297745 27.188592174437176 28.66258062421057 22.05974011205451 32-33 21.914707708770138 26.913623646370638 29.10405743550915 22.067611209350073 34-35 22.132437657961653 26.722488234663533 28.924293814228886 22.220780293145925 36-37 22.406048730174213 27.00115745994281 28.449889758821467 22.142904051061514 38-39 22.17461710143444 27.250528027721092 28.512845052957292 22.062009817887173 40-41 22.026353117285254 26.93032660723232 28.750224840499055 22.29309543498337 42-43 22.161953610821115 26.868933234311033 28.566686014001547 22.402427140866305 44-45 22.13045785580666 27.170128745085503 28.390821637209463 22.30859176189837 46-47 22.26292352072563 27.30826823325353 28.234791497860368 22.194016748160475 48-49 22.336019264923603 27.163090789863798 27.888012249663674 22.61287769554892 50-51 22.3025316357898 27.188924793593554 27.841692122415513 22.666851448201133 52-53 22.315569357298276 27.22529762220932 27.899371968126154 22.55976105236625 54-55 22.39622215118542 27.523692437252343 27.701222745126064 22.378862666436174 56-57 22.156726450253366 27.321981984766147 27.80179428020671 22.719497284773777 58-59 22.159140843125304 26.921204839988526 28.17666498946842 22.74298932741775 60-61 22.45337083046423 27.287263015267655 27.7237973184787 22.535568835789416 62-63 22.18266910166236 27.218935696991764 27.894494894524836 22.703900306821044 64-65 22.263575406801056 27.051425119536592 27.936565690318382 22.748433783343973 66-67 22.062202969316928 27.08051855364346 27.848331702813343 23.00894677422626 68-69 22.393601648744983 27.227506671588824 27.74467099825994 22.63422068140625 70-71 22.39846087246039 27.036571059100247 27.762446379339362 22.802521689100004 72-73 22.259200142160037 27.132768733290824 27.742163995411666 22.86586712913747 74-75 22.41221375520242 27.191583800559272 27.57088547021094 22.825316974027373 76-77 22.34819987696254 27.137462009528164 27.588663749436236 22.92567436407306 78-79 22.38577990201428 27.310803345769063 27.57627791400319 22.727138838213463 80-81 22.26775230646951 27.21351538499426 27.653224614831906 22.865507693704327 82-83 22.177939520834183 27.172624228510006 27.785639318725114 22.8637969319307 84-85 22.228639141944086 27.060575668521242 27.614413249415477 23.09637194011919 86-87 22.308248921604655 27.182600713409283 27.54258895041337 22.96656141457269 88-89 22.09439036942453 27.14367477999206 27.565576231452326 23.196358619131086 90-91 22.02330234546973 27.006836999716782 27.68341434217449 23.286446312638997 92-93 22.116728560091186 27.079115659217486 27.584379437234613 23.219776343456715 94-95 21.936114732724903 26.877360084987206 27.70712009271331 23.47940508957458 96-97 21.9033054466091 26.87435014910732 27.699809419323486 23.522534984960096 98-99 21.895354495600547 26.94680347764866 27.596710770852873 23.56113125589792 100 21.949318030589392 26.828443865848605 27.575939899001117 23.646298204560885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8.0 1 13.5 2 58.5 3 111.5 4 91.0 5 76.0 6 167.5 7 376.5 8 671.5 9 833.0 10 707.0 11 593.0 12 693.0 13 836.0 14 980.5 15 1207.5 16 1457.5 17 1714.5 18 2008.0 19 2352.0 20 2834.5 21 3546.0 22 4558.0 23 6338.5 24 8805.5 25 11711.0 26 16182.0 27 22692.0 28 31761.5 29 42620.5 30 53254.0 31 64077.0 32 75862.5 33 88127.5 34 100510.0 35 112383.0 36 122450.0 37 132232.5 38 139820.0 39 144603.5 40 149589.5 41 153027.5 42 152684.0 43 152762.5 44 152569.0 45 150077.0 46 148706.5 47 144724.0 48 140434.0 49 138658.5 50 135924.0 51 132724.5 52 125982.5 53 120406.0 54 116885.0 55 111039.5 56 102016.0 57 90138.0 58 81609.0 59 76413.0 60 69570.0 61 60612.0 62 52517.5 63 44240.5 64 35468.5 65 27783.0 66 21284.0 67 15522.5 68 11613.5 69 8946.0 70 6057.0 71 3927.0 72 2800.5 73 1906.5 74 1357.5 75 991.0 76 654.5 77 423.0 78 245.5 79 117.5 80 49.5 81 16.5 82 11.0 83 6.5 84 4.5 85 3.0 86 1.0 87 1.5 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04386951848314319 2 0.24264648362993346 3 0.002728263945291789 4 0.0021729535847456725 5 2.8972714463275633E-4 6 4.828785743879273E-5 7 0.0 8 2.6558321591336E-4 9 0.0 10-11 6.156701823446072E-4 12-13 0.0 14-15 2.8972714463275633E-4 16-17 1.4486357231637817E-4 18-19 4.708066100282291E-4 20-21 1.2071964359698183E-5 22-23 1.8107946539547273E-4 24-25 0.0010864767923728362 26-27 1.2071964359698181E-4 28-29 2.6558321591336E-4 30-31 1.0864767923728364E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.243178615818908E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.6558321591336E-4 70-71 2.4143928719396366E-5 72-73 4.104467882297382E-4 74-75 1.4486357231637817E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.2071964359698181E-4 84-85 0.0 86-87 6.277421467043054E-4 88-89 0.0024747526937381275 90-91 0.00239024894322024 92-93 7.484617903012873E-4 94-95 6.760300041430982E-4 96-97 3.0179910899245455E-4 98-99 1.5693553667607636E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4141828.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.553316769016575 #Duplication Level Percentage of deduplicated Percentage of total 1 71.14278114322906 37.387891132489365 2 15.566673842679188 16.36160682988568 3 5.558265510812734 8.763158642281242 4 2.6184147273024005 5.504255143863249 5 1.4696494995587033 3.861747784486761 6 0.8468498342073607 2.670286055573316 7 0.5567207748071437 2.0480266261232516 8 0.4003554797144019 1.6832006676514042 9 0.2832222694570675 1.3395842678535337 >10 1.4322260520724412 13.395396714185607 >50 0.08659090374184844 3.1052777632318826 >100 0.037009484027044896 3.2385210870093792 >500 0.001056703841735163 0.364563976004491 >1k 1.837745487977309E-4 0.27648330936075544 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4510 0.1088891185244776 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 2.4143928719396362E-5 0.0 2.4143928719396362E-5 0.0 4 0.0 2.4143928719396362E-5 0.0 2.4143928719396362E-5 0.0 5 0.0 2.4143928719396362E-5 0.0 2.4143928719396362E-5 0.0 6 0.0 2.4143928719396362E-5 0.0 2.4143928719396362E-5 0.0 7 0.0 2.4143928719396362E-5 0.0 2.4143928719396362E-5 0.0 8 0.0 2.4143928719396362E-5 0.0 2.4143928719396362E-5 0.0 9 0.0 2.4143928719396362E-5 0.0 2.4143928719396362E-5 0.0 10-11 0.0 2.4143928719396362E-5 0.0 3.621589307909454E-5 0.0 12-13 0.0 2.4143928719396362E-5 0.0 4.8287857438792724E-5 0.0 14-15 0.0 2.4143928719396362E-5 0.0 7.243178615818908E-5 0.0 16-17 0.0 2.4143928719396362E-5 0.0 1.0864767923728362E-4 0.0 18-19 0.0 2.4143928719396362E-5 0.0 1.4486357231637817E-4 0.0 20-21 0.0 2.4143928719396362E-5 0.0 1.4486357231637817E-4 0.0 22-23 0.0 4.8287857438792724E-5 0.0 1.5693553667607636E-4 0.0 24-25 0.0 4.8287857438792724E-5 0.0 1.810794653954727E-4 0.0 26-27 0.0 4.8287857438792724E-5 0.0 3.259430377118509E-4 0.0 28-29 0.0 4.8287857438792724E-5 0.0 0.0010744048280131382 0.0 30-31 0.0 4.8287857438792724E-5 0.0 0.004659778242843498 0.0 32-33 0.0 4.8287857438792724E-5 0.0 0.013315376688747094 0.0 34-35 0.0 4.8287857438792724E-5 0.0 0.026039227123868976 0.0 36-37 0.0 4.8287857438792724E-5 0.0 0.04995378852043107 0.0 38-39 0.0 6.035982179849091E-5 0.0 0.08678535178187022 0.0 40-41 0.0 7.243178615818908E-5 0.0 0.12901308311209447 0.0 42-43 0.0 7.243178615818908E-5 0.0 0.174548532676876 0.0 44-45 0.0 7.243178615818908E-5 0.0 0.23051415944843678 0.0 46-47 0.0 7.243178615818908E-5 0.0 0.29773085700323626 0.0 48-49 0.0 7.243178615818908E-5 0.0 0.3737842324693348 0.0 50-51 0.0 7.243178615818908E-5 0.0 0.4529883906333146 0.0 52-53 0.0 7.243178615818908E-5 0.0 0.5349328847069459 0.0 54-55 0.0 7.243178615818908E-5 0.0 0.626885037234767 0.0 56-57 0.0 7.243178615818908E-5 0.0 0.7373193671972859 0.0 58-59 0.0 7.243178615818908E-5 0.0 0.8626987890371112 0.0 60-61 0.0 7.243178615818908E-5 0.0 0.9951403100273599 0.0 62-63 0.0 7.243178615818908E-5 0.0 1.1311551324680793 0.0 64-65 0.0 7.243178615818908E-5 0.0 1.2794954305200505 0.0 66-67 0.0 7.243178615818908E-5 0.0 1.4512915553229153 0.0 68-69 0.0 7.243178615818908E-5 0.0 1.6478472790275212 0.0 70-71 0.0 7.243178615818908E-5 0.0 1.854483092972475 0.0 72-73 0.0 9.657571487758545E-5 0.0 2.0750499537885205 0.0 74-75 0.0 9.657571487758545E-5 0.0 2.305974560025187 0.0 76-77 0.0 9.657571487758545E-5 0.0 2.558906357289583 0.0 78-79 0.0 9.657571487758545E-5 0.0 2.8434787731407485 0.0 80-81 0.0 9.657571487758545E-5 0.0 3.1556235555894645 0.0 82-83 0.0 9.657571487758545E-5 0.0 3.479731654718641 0.0 84-85 0.0 9.657571487758545E-5 0.0 3.8093204256671207 0.0 86-87 0.0 9.657571487758545E-5 0.0 4.163077269263717 0.0 88 0.0 9.657571487758545E-5 0.0 4.451126410850475 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5390 0.0 46.074333 1 GTATCAA 9625 0.0 42.839516 1 TCAACGC 11500 0.0 35.63784 4 ATCAACG 11545 0.0 35.457794 3 CAACGCA 11855 0.0 34.529762 5 AACGCAG 12370 0.0 33.16817 6 TATCAAC 12635 0.0 32.547173 2 ACGCAGA 14565 0.0 28.008265 7 CGCAGAG 14815 0.0 27.408741 8 GTACATG 12210 0.0 27.01591 1 TACATGG 12350 0.0 25.707937 2 GTCGTAC 285 0.0 24.801823 1 GCAGAGT 16400 0.0 24.358585 9 ACATGGG 12975 0.0 23.689606 3 GTGGTAT 2950 0.0 22.842901 1 GAGTACA 9230 0.0 20.830309 1 AGAGTAC 14875 0.0 20.631739 10-11 CATGGGG 8975 0.0 20.527891 4 GAGTACT 10440 0.0 19.829987 12-13 CAGAGTA 15945 0.0 19.586195 10-11 >>END_MODULE