FastQCFastQC Report
Wed 25 May 2016
SRR1033041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451634
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC27200.034.91714
GTATCAA30000.034.009021
ATCAACG28050.033.8593
CAACGCA29000.032.9112855
AACGCAG30800.030.8276866
GGTATCA18250.030.6577151
GTACATG62650.028.7430841
TATCAAC33700.028.7398242
TACATGG63500.027.2471392
ACGCAGA36950.025.315067
CGCAGAG36850.024.8979428
ACATGGG66950.024.649733
CATGGGG43450.022.940444
GTACGAC1954.6622517E-721.7001913
CTGTCGC7850.020.382419
TCTGTCG7600.019.8108338
GAGTACA47450.019.3220881
GTCTCGC5700.018.9718061
GACCGTG3601.10958354E-1018.2795647
GTATTAG11850.018.2513561
Produced by FastQC (version 0.11.5)