FastQCFastQC Report
Wed 25 May 2016
SRR1033041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451634
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46990.19166808748777345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38750.15805785039691894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.057.2593081
GTATCAA67250.043.587841
ATCAACG77400.037.4078523
TCAACGC77700.037.201414
CAACGCA79250.036.5308845
TATCAAC82350.035.4070782
AACGCAG82950.035.2406466
ACGCAGA96400.030.0800137
CGCAGAG97200.029.7853058
GCAGAGT109400.026.3348439
GTACATG63100.024.795681
TACATGG62100.024.1211782
TACACCG2950.023.8972535
TCTACCG1852.8158865E-722.8661883
ACATGGG64650.022.3926373
GAGTACT79750.022.07077612-13
CAGAGTA108300.021.63381810-11
TAGTGCG1100.003060940721.3631329
AGAGTAC101050.021.30225810-11
TGGTATC14550.019.4245642
Produced by FastQC (version 0.11.5)