##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033041_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2451634 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.71386022546596 34.0 31.0 34.0 31.0 34.0 2 32.71865498683735 34.0 31.0 34.0 31.0 34.0 3 32.826861595164694 34.0 31.0 34.0 31.0 34.0 4 36.31044519695843 37.0 37.0 37.0 35.0 37.0 5 36.29592141404467 37.0 37.0 37.0 35.0 37.0 6 36.367003802362014 37.0 37.0 37.0 35.0 37.0 7 36.31753597804566 37.0 37.0 37.0 35.0 37.0 8 36.33037068338912 37.0 37.0 37.0 35.0 37.0 9 38.13506828507028 39.0 39.0 39.0 37.0 39.0 10-11 38.096274974160096 39.0 39.0 39.0 36.0 39.0 12-13 38.071018553340345 39.0 39.0 39.0 35.0 39.0 14-15 39.56769077276624 41.0 40.0 41.0 37.0 41.0 16-17 39.569094530423385 41.0 40.0 41.0 37.0 41.0 18-19 39.589607584166316 41.0 40.0 41.0 37.0 41.0 20-21 39.58367745756503 41.0 40.0 41.0 37.0 41.0 22-23 39.50654583840818 41.0 39.0 41.0 36.5 41.0 24-25 39.45009308077796 41.0 39.0 41.0 36.0 41.0 26-27 39.33883707763883 41.0 39.0 41.0 36.0 41.0 28-29 39.283547217896306 41.0 39.0 41.0 36.0 41.0 30-31 39.273576724747656 41.0 39.0 41.0 36.0 41.0 32-33 39.223507464817345 41.0 39.0 41.0 35.5 41.0 34-35 39.13665355432336 41.0 39.0 41.0 35.0 41.0 36-37 39.038780870227775 41.0 39.0 41.0 35.0 41.0 38-39 38.940779904341355 41.0 39.0 41.0 35.0 41.0 40-41 38.83599162844046 41.0 39.0 41.0 35.0 41.0 42-43 38.713763555245194 41.0 39.0 41.0 35.0 41.0 44-45 38.54194834954973 41.0 38.5 41.0 35.0 41.0 46-47 38.33773862656498 41.0 38.0 41.0 34.5 41.0 48-49 38.11889743738258 40.0 38.0 41.0 34.0 41.0 50-51 37.982345447974694 40.0 38.0 41.0 34.0 41.0 52-53 37.85116538602418 40.0 38.0 41.0 33.5 41.0 54-55 37.70594754355666 40.0 37.5 41.0 33.0 41.0 56-57 37.56091121268509 40.0 37.0 41.0 33.0 41.0 58-59 37.39044429143991 40.0 37.0 41.0 33.0 41.0 60-61 37.19979287283502 40.0 36.0 41.0 33.0 41.0 62-63 36.97014521743458 39.5 36.0 41.0 32.5 41.0 64-65 36.70611416712282 39.0 35.0 41.0 32.0 41.0 66-67 36.43456078680586 39.0 35.0 41.0 32.0 41.0 68-69 36.12976814646885 38.0 35.0 40.5 31.0 41.0 70-71 35.80702074616357 37.0 35.0 40.0 31.0 41.0 72-73 35.468558520562205 37.0 35.0 39.5 31.0 41.0 74-75 35.12793426751301 36.0 35.0 39.0 31.0 41.0 76-77 34.773522271268874 36.0 35.0 39.0 30.5 40.5 78-79 34.41340346887015 35.5 35.0 37.5 30.0 39.5 80-81 34.079882233644994 35.0 34.0 37.0 30.0 39.0 82-83 33.77297855226351 35.0 34.0 37.0 30.0 39.0 84-85 33.53936415468215 35.0 34.0 36.0 30.0 38.0 86-87 33.32223243763139 35.0 34.0 36.0 30.0 37.0 88-89 33.10371287068135 35.0 34.0 36.0 30.0 37.0 90-91 32.91303534703793 35.0 34.0 35.0 29.0 36.5 92-93 32.731084248301336 35.0 34.0 35.0 29.0 36.0 94-95 32.56773931182224 35.0 34.0 35.0 29.0 36.0 96-97 32.4183813734024 35.0 34.0 35.0 29.0 36.0 98-99 32.28843803765162 35.0 34.0 35.0 29.0 36.0 100 32.18051267032518 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.072761325242368 1101 2 -0.03120710542282268 1101 3 0.05736743833768543 1101 4 0.10470825465699818 1101 5 0.07166976165355265 1101 6 0.07549488365165047 1101 7 0.15351570377853108 1101 8 0.186564729651991 1101 9 0.1993574693989828 1101 10-11 0.2035428145511915 1101 12-13 0.21975754107339185 1101 14-15 0.3201838855580377 1101 16-17 0.30424957761272964 1101 18-19 0.2592836233625704 1101 20-21 0.24791850807598337 1101 22-23 0.2678179231175122 1101 24-25 0.3268135257695448 1101 26-27 0.34797838704177764 1101 28-29 0.3166974063353152 1101 30-31 0.31598760930081937 1101 32-33 0.37456356543898295 1101 34-35 0.3018633445843264 1101 36-37 0.2731125984069891 1101 38-39 0.24482771481536503 1101 40-41 0.3734263310681527 1101 42-43 0.3204423651698889 1101 44-45 0.32303595658745365 1101 46-47 0.31584391318870786 1101 48-49 0.19875670184953265 1101 50-51 0.2593031295052626 1101 52-53 0.32491521199091977 1101 54-55 0.3721986517771896 1101 56-57 0.2652264517160674 1101 58-59 0.3100065899495874 1101 60-61 0.3159756915946943 1101 62-63 0.28520788185662127 1101 64-65 0.3032728341772071 1101 66-67 0.2588331361336458 1101 68-69 0.279774077276862 1101 70-71 0.2125612784682005 1101 72-73 0.1597325248451824 1101 74-75 0.17960308829061944 1101 76-77 0.16852749240751308 1101 78-79 0.20149537163995745 1101 80-81 0.2695135913600595 1101 82-83 0.2096793861410262 1101 84-85 0.2609477046810511 1101 86-87 0.27122986877574107 1101 88-89 0.2668153857705349 1101 90-91 0.25380803196129165 1101 92-93 0.16856583670902126 1101 94-95 0.2565739813804129 1101 96-97 0.28602316210490386 1101 98-99 0.262797592389596 1101 100 0.32795743300375335 1102 1 0.11010059231734459 1102 2 0.045831499437106515 1102 3 0.09167402113168066 1102 4 0.06790207661579473 1102 5 0.12062745506850092 1102 6 0.135455835585077 1102 7 0.09792956063350289 1102 8 0.13016043116801512 1102 9 0.11193133795747201 1102 10-11 0.15178790801664377 1102 12-13 0.1880727785255374 1102 14-15 0.22159861171480344 1102 16-17 0.21117430074018984 1102 18-19 0.23308295548756774 1102 20-21 0.23765706128175168 1102 22-23 0.25047052902030487 1102 24-25 0.2681812535936601 1102 26-27 0.2751700364042051 1102 28-29 0.2701861873026701 1102 30-31 0.23772271162476954 1102 32-33 0.24211994548051052 1102 34-35 0.23867160985243885 1102 36-37 0.1567993876333631 1102 38-39 0.1512447811107691 1102 40-41 0.18692597877809902 1102 42-43 0.19213845891533765 1102 44-45 0.20318442611421972 1102 46-47 0.16514975041020108 1102 48-49 0.2482513390866785 1102 50-51 0.2716348797427983 1102 52-53 0.1845159198429016 1102 54-55 0.16860785994536087 1102 56-57 0.13440668488752294 1102 58-59 0.176864313093418 1102 60-61 0.22731997666400616 1102 62-63 0.1741088835563005 1102 64-65 0.20401997679177697 1102 66-67 0.2072553618283166 1102 68-69 0.2588587004130005 1102 70-71 0.20910118276790968 1102 72-73 0.1667068243125982 1102 74-75 0.1713798843822758 1102 76-77 0.13188274538411804 1102 78-79 0.16904176683926408 1102 80-81 0.14200640406002663 1102 82-83 0.13923665334800717 1102 84-85 0.19228358067043416 1102 86-87 0.21629908667301834 1102 88-89 0.22084703377156245 1102 90-91 0.13885537346414623 1102 92-93 0.25451661839429107 1102 94-95 0.2575911754291411 1102 96-97 0.26327948729560546 1102 98-99 0.26120175699018944 1102 100 0.2677065257651918 1103 1 0.051382183280459515 1103 2 0.0892849760115979 1103 3 0.13581040921711462 1103 4 0.045227270501499106 1103 5 0.053018584911860955 1103 6 0.07841664478792154 1103 7 0.11518710655347064 1103 8 0.11338527426546818 1103 9 0.1823891408993461 1103 10-11 0.18692474570125484 1103 12-13 0.1613613537249421 1103 14-15 0.21544515651675766 1103 16-17 0.22885937575011184 1103 18-19 0.18877915003726287 1103 20-21 0.2586677829294075 1103 22-23 0.2367546012916364 1103 24-25 0.2288586665003649 1103 26-27 0.26158715976400515 1103 28-29 0.30479649579832824 1103 30-31 0.2649158948689063 1103 32-33 0.20120326762115326 1103 34-35 0.22977641942858185 1103 36-37 0.24189060329782563 1103 38-39 0.29312768732487626 1103 40-41 0.2607012308972969 1103 42-43 0.25347910942796403 1103 44-45 0.25149142983523376 1103 46-47 0.22348413995478467 1103 48-49 0.25109743428946274 1103 50-51 0.27745255435028326 1103 52-53 0.28658376076423764 1103 54-55 0.24958489633640824 1103 56-57 0.3009434533351154 1103 58-59 0.31514853418018873 1103 60-61 0.3293323270840034 1103 62-63 0.28037067134674487 1103 64-65 0.3081989790599522 1103 66-67 0.25234170946243495 1103 68-69 0.32612043964926585 1103 70-71 0.3326246152085446 1103 72-73 0.3342670141657891 1103 74-75 0.3713553550583839 1103 76-77 0.3319830696882633 1103 78-79 0.31009736012415345 1103 80-81 0.2680634823464061 1103 82-83 0.283194462910906 1103 84-85 0.31338970814599065 1103 86-87 0.3034311546968027 1103 88-89 0.31590982947130186 1103 90-91 0.3005004650455376 1103 92-93 0.35974426502321677 1103 94-95 0.32045150899011077 1103 96-97 0.22076461993997754 1103 98-99 0.26088647005069276 1103 100 0.2992226082366898 1104 1 0.05420700719155036 1104 2 0.09809052155836184 1104 3 0.1078254419350202 1104 4 0.05946870646817359 1104 5 0.05271615010462938 1104 6 0.08834808283437212 1104 7 0.049323689252354086 1104 8 0.11162026611068399 1104 9 0.15400340005354707 1104 10-11 0.13465433240847346 1104 12-13 0.07667551025315333 1104 14-15 0.14482201400863914 1104 16-17 0.2025342631630238 1104 18-19 0.20869826899791377 1104 20-21 0.18288129500234618 1104 22-23 0.1543189038399646 1104 24-25 0.2068133601100115 1104 26-27 0.1705100734792495 1104 28-29 0.2170630997582208 1104 30-31 0.21473893255137 1104 32-33 0.20319665459856395 1104 34-35 0.1361915654603436 1104 36-37 0.16104946015637012 1104 38-39 0.23205504957493162 1104 40-41 0.2560500233706904 1104 42-43 0.22202916743488998 1104 44-45 0.246767072075734 1104 46-47 0.21525611894721663 1104 48-49 0.12791645328100287 1104 50-51 0.18953813736238345 1104 52-53 0.22264592845579756 1104 54-55 0.13962090298583263 1104 56-57 0.1416490304624176 1104 58-59 0.16727564030090036 1104 60-61 0.2216788632005091 1104 62-63 0.08492329858307812 1104 64-65 0.1560324339221637 1104 66-67 0.13099560888311146 1104 68-69 0.18386040282053528 1104 70-71 0.17625564094819168 1104 72-73 0.10289442336827648 1104 74-75 0.14453179436900854 1104 76-77 0.09608637852000612 1104 78-79 0.10996285168293696 1104 80-81 0.15470874279358782 1104 82-83 0.1831479762829531 1104 84-85 0.2088040252855521 1104 86-87 0.15901331539078 1104 88-89 0.12788474687752682 1104 90-91 0.10253247155038991 1104 92-93 0.08081850207189234 1104 94-95 0.024824935408261695 1104 96-97 0.05397471353345651 1104 98-99 0.10838656515462475 1104 100 0.10805646499387223 1105 1 0.018740456591800125 1105 2 0.013227229089515902 1105 3 0.06104037193991729 1105 4 0.03900692484211277 1105 5 0.019192767170757463 1105 6 0.07407698933206319 1105 7 0.08243734111910328 1105 8 0.12150909091819528 1105 9 0.10167943769393872 1105 10-11 0.1394493247284032 1105 12-13 0.1374997089417178 1105 14-15 0.12986142682885315 1105 16-17 0.14853014573570533 1105 18-19 0.13235857143580176 1105 20-21 0.12896620617574683 1105 22-23 0.1634958739115575 1105 24-25 0.1947793699711582 1105 26-27 0.19265712245960742 1105 28-29 0.1321249517759 1105 30-31 0.15865128817499397 1105 32-33 0.1694689048907989 1105 34-35 0.2545368853739376 1105 36-37 0.28272308646001676 1105 38-39 0.28896573052984564 1105 40-41 0.2653095381673154 1105 42-43 0.2862577333628522 1105 44-45 0.29612328972181956 1105 46-47 0.3909373955591562 1105 48-49 0.3939636299604672 1105 50-51 0.34583088002683837 1105 52-53 0.368592691513868 1105 54-55 0.34779662133691147 1105 56-57 0.3152848105499686 1105 58-59 0.3445427232767244 1105 60-61 0.3095044971531351 1105 62-63 0.3271726564715749 1105 64-65 0.34105419065014075 1105 66-67 0.3243018468203971 1105 68-69 0.34319570414867684 1105 70-71 0.3675338805127666 1105 72-73 0.35497653716494426 1105 74-75 0.29271453858127927 1105 76-77 0.28589306924794755 1105 78-79 0.27665086803427386 1105 80-81 0.3860943669771757 1105 82-83 0.3833758762005033 1105 84-85 0.3171734868295957 1105 86-87 0.29319259222584293 1105 88-89 0.2613575306431031 1105 90-91 0.2868316086462883 1105 92-93 0.39467686363641263 1105 94-95 0.3528266664368189 1105 96-97 0.34140292747009937 1105 98-99 0.3071207629936339 1105 100 0.29736792893496045 1106 1 0.04005444467999553 1106 2 0.08875757548420182 1106 3 0.11396526009923491 1106 4 0.07118329645751942 1106 5 -5.839141451886576E-4 1106 6 0.00666808213026826 1106 7 0.02486915259915179 1106 8 0.046888135041058376 1106 9 0.060888692126169985 1106 10-11 0.10483713634091885 1106 12-13 0.08312337548696291 1106 14-15 0.09826598024667277 1106 16-17 0.12520696755225202 1106 18-19 0.09799671380027775 1106 20-21 0.06247977765049484 1106 22-23 0.10553172915967224 1106 24-25 0.04964950547302749 1106 26-27 0.0568002186134251 1106 28-29 0.07844851945035458 1106 30-31 -0.04464330105392378 1106 32-33 -0.015012007685029971 1106 34-35 -0.04833699050301021 1106 36-37 0.006908427406564499 1106 38-39 0.05375432522424717 1106 40-41 0.024634063011944818 1106 42-43 -0.02805708574459942 1106 44-45 -0.03657022186278169 1106 46-47 -0.06958004401848683 1106 48-49 -0.01471374061262054 1106 50-51 -0.0022933344865094796 1106 52-53 0.08254656127249405 1106 54-55 0.030210945144276025 1106 56-57 0.04514008389538304 1106 58-59 -0.014994050507851853 1106 60-61 0.03531434670237843 1106 62-63 0.10017690933480594 1106 64-65 0.026934747795721137 1106 66-67 -0.01096656293674414 1106 68-69 0.057725689436331606 1106 70-71 0.0556943791874005 1106 72-73 0.06956329037114983 1106 74-75 0.134041031380427 1106 76-77 0.019183747798031447 1106 78-79 0.016251852642277242 1106 80-81 0.06688276298386597 1106 82-83 0.0923361402344014 1106 84-85 0.06073900094983031 1106 86-87 -0.006501255235605186 1106 88-89 0.019243146440985015 1106 90-91 -0.05386302568158641 1106 92-93 -0.008177616535022025 1106 94-95 -0.014576485128792172 1106 96-97 0.028999137265401487 1106 98-99 0.06510375154070402 1106 100 0.0635198998067068 1107 1 0.04691419564657906 1107 2 5.998647905087751E-4 1107 3 0.03096414336079789 1107 4 0.06578585201582143 1107 5 0.07706560950192198 1107 6 0.06138706870322608 1107 7 0.05805902281664288 1107 8 0.05450768701427222 1107 9 0.05722178086910645 1107 10-11 0.08184885498865668 1107 12-13 0.12041510900459684 1107 14-15 0.16661078389742556 1107 16-17 0.0893156449764021 1107 18-19 0.15175184963771216 1107 20-21 0.14635663274152932 1107 22-23 0.10805142167887283 1107 24-25 0.07474006749988149 1107 26-27 0.0805106026353144 1107 28-29 0.14144681629333888 1107 30-31 0.13285845857578948 1107 32-33 0.11567081092388776 1107 34-35 0.12344434567864226 1107 36-37 0.05831530840023902 1107 38-39 0.07911345050315077 1107 40-41 0.04862991469891398 1107 42-43 0.13057029835732692 1107 44-45 0.1774669483983189 1107 46-47 0.1072388446435113 1107 48-49 0.009734453719318026 1107 50-51 0.1135921762623795 1107 52-53 0.14855414282693857 1107 54-55 0.10280272077334729 1107 56-57 0.08485666570339134 1107 58-59 0.11501158180321625 1107 60-61 0.11345540729722359 1107 62-63 0.09037825884551154 1107 64-65 0.06358512339532751 1107 66-67 0.03510925709558421 1107 68-69 0.039307583463617846 1107 70-71 0.05166241488711876 1107 72-73 0.11200612016989453 1107 74-75 0.06023415756737904 1107 76-77 0.01780566414512208 1107 78-79 0.06450972572304892 1107 80-81 0.007975895079894713 1107 82-83 0.05172063260459936 1107 84-85 0.12384182839990387 1107 86-87 0.1757003426054098 1107 88-89 0.18542755114759046 1107 90-91 0.11382912550968882 1107 92-93 0.1728261241273401 1107 94-95 0.15861203123968437 1107 96-97 0.13748934130764923 1107 98-99 0.16917752808380726 1107 100 0.20665480076255704 1108 1 0.01966084220660491 1108 2 0.0913203737304471 1108 3 0.03412345860930088 1108 4 -0.025127533666257307 1108 5 -0.005692114086606637 1108 6 0.03104703626723193 1108 7 0.01593194974435619 1108 8 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40-41 -0.2266558228597546 2214 42-43 -0.2952619657131166 2214 44-45 -0.3153058377620326 2214 46-47 -0.3073661124954654 2214 48-49 -0.22508005308802126 2214 50-51 -0.24887782878010256 2214 52-53 -0.2838495032690247 2214 54-55 -0.22633954678803292 2214 56-57 -0.21546046706880873 2214 58-59 -0.18892179549014543 2214 60-61 -0.15114311099143407 2214 62-63 -0.19698483800875977 2214 64-65 -0.1825700141090394 2214 66-67 -0.13820560268487725 2214 68-69 -0.12728021375139065 2214 70-71 -0.0914217168377931 2214 72-73 -0.12056232946355294 2214 74-75 -0.1744285475255154 2214 76-77 -0.17523451272931823 2214 78-79 -0.16115562472882772 2214 80-81 -0.16990527322234783 2214 82-83 -0.1707355638191217 2214 84-85 -0.24114025918397886 2214 86-87 -0.2404198699542235 2214 88-89 -0.23166106449959045 2214 90-91 -0.22533000718492957 2214 92-93 -0.21283357875462627 2214 94-95 -0.2224267802881741 2214 96-97 -0.25168563251027365 2214 98-99 -0.20596354479931733 2214 100 -0.183912764498686 2215 1 -0.22349235791671873 2215 2 -0.17456943480264897 2215 3 -0.21697569945860806 2215 4 -0.10265288904570014 2215 5 -0.15784722792837869 2215 6 -0.17762826416252864 2215 7 -0.22344887376974043 2215 8 -0.17718616909932905 2215 9 -0.18580753153784713 2215 10-11 -0.23463585658629427 2215 12-13 -0.22815000448733258 2215 14-15 -0.34339921845953825 2215 16-17 -0.33493951326753546 2215 18-19 -0.30962116651427607 2215 20-21 -0.4053699724253761 2215 22-23 -0.44521065707030516 2215 24-25 -0.4042057295211734 2215 26-27 -0.35834678449123203 2215 28-29 -0.44545157641519495 2215 30-31 -0.42858403006471946 2215 32-33 -0.3286967058507244 2215 34-35 -0.28700909511326955 2215 36-37 -0.3286409699261341 2215 38-39 -0.3271522686068593 2215 40-41 -0.32421066756631944 2215 42-43 -0.25247705594036773 2215 44-45 -0.27039283414011805 2215 46-47 -0.3259788372622836 2215 48-49 -0.3519728637550372 2215 50-51 -0.2792571101673218 2215 52-53 -0.2891261595721133 2215 54-55 -0.3012032539581355 2215 56-57 -0.30422600442577163 2215 58-59 -0.3203649314880863 2215 60-61 -0.294678557640367 2215 62-63 -0.3161350671827705 2215 64-65 -0.33088815251090864 2215 66-67 -0.34253902362785027 2215 68-69 -0.36649621426422385 2215 70-71 -0.4155728755287811 2215 72-73 -0.338507494372692 2215 74-75 -0.3329369356148817 2215 76-77 -0.3227605488202201 2215 78-79 -0.3452578844166396 2215 80-81 -0.33969604856771696 2215 82-83 -0.3321281469147337 2215 84-85 -0.2900503909189709 2215 86-87 -0.3093316684092997 2215 88-89 -0.333357708622799 2215 90-91 -0.29085241495440783 2215 92-93 -0.27255244153869285 2215 94-95 -0.24865946723327426 2215 96-97 -0.22545786879990004 2215 98-99 -0.17293530671476276 2215 100 -0.21143385171301787 2216 1 -0.2597164326725263 2216 2 -0.19179552836987313 2216 3 -0.2181288495765159 2216 4 -0.14023182563720837 2216 5 -0.21713512796029732 2216 6 -0.18344130833217065 2216 7 -0.1595460497330805 2216 8 -0.18547789061711484 2216 9 -0.20667663457419394 2216 10-11 -0.24590951820097473 2216 12-13 -0.26543992744527145 2216 14-15 -0.3957032798743967 2216 16-17 -0.3617673653620628 2216 18-19 -0.30964992023646687 2216 20-21 -0.3556877760863415 2216 22-23 -0.3219563020794638 2216 24-25 -0.3594503188960019 2216 26-27 -0.3196750370321766 2216 28-29 -0.40996473935125977 2216 30-31 -0.3544469866298954 2216 32-33 -0.3637130516640994 2216 34-35 -0.39666894196823677 2216 36-37 -0.46872121411011136 2216 38-39 -0.40210390402321394 2216 40-41 -0.3730065086356831 2216 42-43 -0.39683533603211885 2216 44-45 -0.43543084932004916 2216 46-47 -0.32710141839775986 2216 48-49 -0.39861631445341317 2216 50-51 -0.4275610157118237 2216 52-53 -0.40526580778435317 2216 54-55 -0.37122486039567804 2216 56-57 -0.42571720924787826 2216 58-59 -0.3729745617098956 2216 60-61 -0.5003596928992806 2216 62-63 -0.4917217774447309 2216 64-65 -0.5182108832722392 2216 66-67 -0.5034795123782061 2216 68-69 -0.4885223157701901 2216 70-71 -0.45548582532908455 2216 72-73 -0.4775392442424078 2216 74-75 -0.516264762658821 2216 76-77 -0.428659200662743 2216 78-79 -0.5710416472284479 2216 80-81 -0.5451412726059175 2216 82-83 -0.4566038331980735 2216 84-85 -0.49987954104559407 2216 86-87 -0.5076584583345607 2216 88-89 -0.5870300890025959 2216 90-91 -0.5567970437082792 2216 92-93 -0.5762203563783004 2216 94-95 -0.44016288754661304 2216 96-97 -0.5616594343287389 2216 98-99 -0.70392501806176 2216 100 -0.6905878145834414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 2.0 4 8.0 5 20.0 6 65.0 7 154.0 8 324.0 9 608.0 10 1109.0 11 1838.0 12 3023.0 13 4600.0 14 5523.0 15 6928.0 16 8508.0 17 12097.0 18 14141.0 19 7457.0 20 4968.0 21 4691.0 22 4717.0 23 5069.0 24 5718.0 25 7030.0 26 8744.0 27 11511.0 28 14799.0 29 19158.0 30 24560.0 31 31342.0 32 39935.0 33 52220.0 34 72477.0 35 110371.0 36 200328.0 37 459570.0 38 863294.0 39 442836.0 40 1877.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78529248174578 20.139365826725577 13.485006706715163 23.59033498481348 2 15.625641424032906 20.538244205206627 40.36835627808466 23.467758092675805 3 17.910312606308906 25.81019113372508 30.468326166532467 25.811170093433546 4 12.043226195142784 17.129030429084395 39.55670837736976 31.271034998403064 5 12.005823071780496 37.86379412528904 36.17956565170803 13.950817151222433 6 30.00124815020876 39.02341627685767 17.77333265895152 13.202002913982055 7 26.285489595918477 32.91274309297391 23.123924696753267 17.677842614354343 8 24.68109875058176 36.1150846725493 20.879206043674778 18.32461053319416 9 24.741743669732106 16.34811721488607 21.988885779851316 36.92125333553051 10-11 23.34721665901187 26.721248218526345 29.485764934398535 20.44577018806325 12-13 24.532352708438534 24.209445618717965 29.638702188010118 21.61949948483338 14-15 21.65051583081035 26.148935757981178 27.249837098009383 24.95071131319909 16-17 20.558840103008954 29.651315868636864 27.55758767171983 22.23225635663435 18-19 20.252518897210148 27.989570971176796 30.630989283202098 21.126920848410958 20-21 21.61586925291459 27.431113290156688 30.203978244713525 20.7490392122152 22-23 21.141249348902303 27.319284443119248 30.350595461221907 21.188870746756542 24-25 20.977871883756364 27.365777461704237 30.346309230731894 21.3100414238075 26-27 20.796629018932503 27.208015237206425 30.496540975485498 21.498814768375578 28-29 20.820915566938467 27.316638715857643 30.470807465323556 21.391638251880334 30-31 21.162878204624878 27.906099303219385 29.933034416863048 20.997988075292685 32-33 20.654408447590463 27.550135950145904 30.555927189784445 21.239528412479185 34-35 20.846831133847875 27.212707932750156 30.502473044508278 21.43798788889369 36-37 21.376926572237128 27.730994920122658 29.768452387264983 21.123626120375228 38-39 21.14432660013689 27.906347358537204 29.885455985681386 21.06387005564452 40-41 20.942469774228034 27.54566038228397 30.100100626806704 21.41176921668129 42-43 20.93854547620077 27.522746054264218 29.906401200179143 21.632307269355866 44-45 21.152198900814724 27.782042507160533 29.735005306664863 21.330753285359886 46-47 21.25078621033972 28.200681667818277 29.21290453632149 21.335627585520513 48-49 21.28464118216667 27.916320299033217 29.19848558145302 21.600552937347093 50-51 21.271038009751862 27.893131682787885 29.03769485983634 21.798135447623913 52-53 21.30016144334758 27.93135109074193 28.989788035245066 21.778699430665426 54-55 21.476044140357004 28.451493983196514 28.72939027603631 21.343071600410177 56-57 21.204612923462474 28.094120084808743 28.799894274594006 21.901372717134777 58-59 21.052632652345334 27.64254370758441 29.311553029530508 21.993270610539746 60-61 21.458994287075477 28.147716992014303 28.776807631155382 21.61648108975483 62-63 21.25484472804668 28.04615207653345 28.874456790858666 21.824546404561204 64-65 21.238488289850768 27.740988255180017 29.108239647516715 21.9122838074525 66-67 21.102517749386735 27.84542880381003 28.912798566180758 22.139254880622474 68-69 21.66872652072295 28.06616414385531 28.68836243641985 21.57674689900189 70-71 21.63210773551593 27.768363419736268 28.7730812945736 21.8264475501742 72-73 21.350433813952936 27.958800421108105 28.943554627192476 21.747211137746483 74-75 21.421546953213205 28.007384468794665 28.73873403512436 21.83233454286777 76-77 21.516629317426663 28.073582761537814 28.493282439385325 21.916505481650198 78-79 21.600450964540386 28.21263288076442 28.571495582130126 21.61542057256507 80-81 21.47547309263944 27.937102357040246 28.73414220882889 21.85328234149143 82-83 21.272519433479527 28.03438117254523 28.866377811519467 21.82672158245577 84-85 21.322779011875344 27.954091026637744 28.711422667494414 22.011707293992497 86-87 21.518503033702135 28.132463162188344 28.489512058328657 21.85952174578086 88-89 21.131659411446062 28.078641914072954 28.66522568413358 22.124472990347403 90-91 21.080334618974945 27.904446323969857 28.726146099766087 22.28907295728911 92-93 21.288518376813393 28.086731967652433 28.52303495400188 22.10171470153229 94-95 21.062010974364938 27.862405407031467 28.72787307181265 22.347710546790953 96-97 20.98190103571825 27.87070000717889 28.746647131380236 22.40075182572262 98-99 21.041566988207453 27.918187591699084 28.647191484206648 22.393053935886815 100 21.19871889523477 27.89400864892557 28.4786391443421 22.428633311497556 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 20.0 1 26.0 2 127.0 3 234.5 4 162.0 5 109.0 6 280.0 7 609.5 8 1050.0 9 1262.0 10 1019.0 11 816.5 12 891.5 13 1057.5 14 1262.0 15 1484.0 16 1676.5 17 1994.0 18 2449.0 19 2871.5 20 3432.0 21 4340.5 22 5669.5 23 7780.0 24 10693.0 25 14092.5 26 18265.0 27 23441.5 28 30046.0 29 37488.5 30 44319.5 31 51649.5 32 59347.5 33 66394.5 34 72788.5 35 78190.0 36 82056.0 37 85625.0 38 88079.0 39 88752.5 40 89591.5 41 89595.0 42 87487.5 43 85825.0 44 84245.0 45 81923.0 46 80713.0 47 78754.5 48 76232.0 49 74262.5 50 72172.5 51 69467.0 52 64833.0 53 60862.5 54 58234.0 55 55121.5 56 51494.0 57 46704.0 58 42388.5 59 38470.0 60 33958.5 61 29013.0 62 24122.0 63 19758.5 64 15937.5 65 12642.0 66 9761.5 67 7171.0 68 5370.5 69 4146.5 70 2900.0 71 1945.0 72 1415.5 73 1066.0 74 794.0 75 526.5 76 336.0 77 236.5 78 149.5 79 79.5 80 41.0 81 18.5 82 8.0 83 2.0 84 3.0 85 2.5 86 0.5 87 1.5 88 2.0 89 2.5 90 2.0 91 0.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04507198056479882 2 0.24081898032087987 3 0.002121034379519945 4 0.002651292974399931 5 2.85523858781531E-4 6 2.447347360984551E-4 7 0.0 8 5.302585948799862E-4 9 0.0 10-11 4.894694721969102E-4 12-13 0.0 14-15 2.651292974399931E-4 16-17 1.0197280670768964E-4 18-19 6.322314015876758E-4 20-21 0.0 22-23 2.0394561341537928E-4 24-25 9.993335057353586E-4 26-27 1.427619293907655E-4 28-29 2.2434017475691723E-4 30-31 6.118368402461378E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.0789122683075855E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.6315649073230342E-4 70-71 2.0394561341537928E-5 72-73 2.85523858781531E-4 74-75 8.157824536615171E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.118368402461378E-5 84-85 0.0 86-87 3.6710210414768273E-4 88-89 0.0026920820970830067 90-91 0.0028348440264737724 92-93 4.894694721969102E-4 94-95 4.690749108553724E-4 96-97 8.157824536615171E-5 98-99 4.0789122683075855E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2451634.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.79428411333126 #Duplication Level Percentage of deduplicated Percentage of total 1 81.39373136122742 57.62210943030895 2 12.281889693331584 17.389751767966224 3 3.1919814193469223 6.779221184571613 4 1.1815821585829733 3.34597052151865 5 0.6173204177740614 2.185137852242863 6 0.33363718171053436 1.417176325967202 7 0.20517408555526542 1.0167606753844676 8 0.15408420333517728 0.8726624694629476 9 0.11435832635046399 0.7286324261741824 >10 0.49380711392636756 6.049380029560825 >50 0.025206743313780523 1.215437630016692 >100 0.0068238726367071175 0.8315693978477235 >500 1.7289553236985125E-4 0.06838088626073412 >1k 2.3052737647887185E-4 0.477809402716749 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4699 0.19166808748777345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3875 0.15805785039691894 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.078912268307586E-5 0.0 3 0.0 0.0 0.0 8.157824536615172E-5 0.0 4 0.0 0.0 0.0 8.157824536615172E-5 0.0 5 0.0 0.0 0.0 8.157824536615172E-5 0.0 6 0.0 8.157824536615172E-5 0.0 8.157824536615172E-5 0.0 7 0.0 8.157824536615172E-5 0.0 8.157824536615172E-5 0.0 8 0.0 8.157824536615172E-5 0.0 8.157824536615172E-5 0.0 9 0.0 8.157824536615172E-5 0.0 8.157824536615172E-5 0.0 10-11 0.0 8.157824536615172E-5 0.0 8.157824536615172E-5 0.0 12-13 0.0 8.157824536615172E-5 0.0 1.427619293907655E-4 0.0 14-15 0.0 8.157824536615172E-5 0.0 2.651292974399931E-4 0.0 16-17 0.0 8.157824536615172E-5 0.0 3.263129814646069E-4 0.0 18-19 0.0 8.157824536615172E-5 0.0 3.467075428061448E-4 0.0 20-21 0.0 8.157824536615172E-5 0.0 3.6710210414768273E-4 0.0 22-23 0.0 8.157824536615172E-5 0.0 4.282857881722965E-4 0.0 24-25 0.0 8.157824536615172E-5 0.0 4.690749108553724E-4 0.0 26-27 0.0 8.157824536615172E-5 0.0 6.322314015876758E-4 0.0 28-29 0.0 8.157824536615172E-5 0.0 0.0013052519258584276 0.0 30-31 0.0 8.157824536615172E-5 0.0 0.0035690482347691373 0.0 32-33 0.0 8.157824536615172E-5 0.0 0.009483471023815138 0.0 34-35 0.0 8.157824536615172E-5 0.0 0.01862023450482413 0.0 36-37 0.0 8.157824536615172E-5 0.0 0.03418128480841757 0.0 38-39 0.0 8.157824536615172E-5 0.0 0.058899493154361535 0.0 40-41 0.0 8.157824536615172E-5 0.0 0.09283604322668065 0.0 42-43 0.0 8.157824536615172E-5 0.0 0.12948506995742431 0.0 44-45 0.0 8.157824536615172E-5 0.0 0.1684590766811033 0.0 46-47 0.0 8.157824536615172E-5 0.0 0.2159376154842036 0.0 48-49 0.0 8.157824536615172E-5 0.0 0.27302199267916827 0.0 50-51 0.0 8.157824536615172E-5 0.0 0.33634710564464354 0.0 52-53 0.0 8.157824536615172E-5 0.0 0.39828538843889427 0.0 54-55 0.0 8.157824536615172E-5 0.0 0.4684222848924432 0.0 56-57 0.0 8.157824536615172E-5 0.0 0.5500005302585949 0.0 58-59 0.0 1.0197280670768965E-4 0.0 0.6426734169945432 0.0 60-61 0.0 1.6315649073230345E-4 0.0 0.7435041282671068 0.0 62-63 0.0 1.6315649073230345E-4 0.0 0.8504532079421316 0.0 64-65 0.0 1.6315649073230345E-4 0.0 0.9661719489940179 0.0 66-67 0.0 1.6315649073230345E-4 0.0 1.0972885838587652 0.0 68-69 0.0 1.6315649073230345E-4 0.0 1.2414781325434383 0.0 70-71 0.0 1.8355105207384136E-4 0.0 1.3986590168026711 0.0 72-73 0.0 2.0394561341537928E-4 0.0 1.5778456327494235 0.0 74-75 0.0 2.0394561341537928E-4 0.0 1.7570526432575173 0.0 76-77 0.0 2.0394561341537928E-4 0.0 1.9483536286411431 0.0 78-79 0.0 2.0394561341537928E-4 0.0 2.173468796729039 0.0 80-81 0.0 2.0394561341537928E-4 0.0 2.418733791422374 0.0 82-83 0.0 2.0394561341537928E-4 0.0 2.6724217399497645 0.0 84-85 0.0 2.0394561341537928E-4 0.0 2.925987321109105 0.0 86-87 0.0 2.4473473609845517E-4 0.0 3.189505448203117 0.0 88 0.0 2.4473473609845517E-4 0.0 3.4059325331595174 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3465 0.0 57.259308 1 GTATCAA 6725 0.0 43.58784 1 ATCAACG 7740 0.0 37.407852 3 TCAACGC 7770 0.0 37.20141 4 CAACGCA 7925 0.0 36.530884 5 TATCAAC 8235 0.0 35.407078 2 AACGCAG 8295 0.0 35.240646 6 ACGCAGA 9640 0.0 30.080013 7 CGCAGAG 9720 0.0 29.785305 8 GCAGAGT 10940 0.0 26.334843 9 GTACATG 6310 0.0 24.79568 1 TACATGG 6210 0.0 24.121178 2 TACACCG 295 0.0 23.897253 5 TCTACCG 185 2.8158865E-7 22.866188 3 ACATGGG 6465 0.0 22.392637 3 GAGTACT 7975 0.0 22.070776 12-13 CAGAGTA 10830 0.0 21.633818 10-11 TAGTGCG 110 0.0030609407 21.363132 9 AGAGTAC 10105 0.0 21.302258 10-11 TGGTATC 1455 0.0 19.424564 2 >>END_MODULE