FastQCFastQC Report
Wed 25 May 2016
SRR1033040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4518607
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57400.033.994091
TCAACGC56450.033.5636984
ATCAACG57250.033.1764373
GGTATCA36900.032.7471851
CAACGCA59350.032.1620455
AACGCAG63050.030.2656046
GTACATG132050.027.6306151
TATCAAC71000.027.1487242
TACATGG132600.026.272332
ACATGGG136900.024.3825323
ACGCAGA78400.023.9207537
CGCAGAG81450.022.8708958
CATGGGG98500.020.5716784
GAGTACA93900.019.1778721
GCAGAGT97050.018.1797129
TATACTG24100.017.1673775
AGTACAT97450.017.1265722
GTATAGA16200.016.253251
GTATTAA31100.015.8743561
GTATATA30650.015.8006141
Produced by FastQC (version 0.11.5)