FastQCFastQC Report
Wed 25 May 2016
SRR1033040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4518607
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61250.13555062434064302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51340.11361908659018145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56700.051.54391
GTATCAA104950.043.6119421
ATCAACG127700.035.001193
TCAACGC128350.034.7869344
CAACGCA129650.034.401115
AACGCAG136450.032.7552536
TATCAAC138100.032.717972
ACGCAGA157250.028.1835067
CGCAGAG159200.027.7205168
GTACATG133250.026.0009211
TACATGG132950.024.9840132
GCAGAGT181150.024.0502829
ACATGGG140800.022.598483
AGAGTAC167150.020.77862710-11
GAGTACT112250.020.07606712-13
CAGAGTA177650.019.62987310-11
CATGGGG103350.018.9178264
AGTACTT117950.018.86680612-13
ACACCGT7050.018.6656426
GAGTACA101000.018.1083561
Produced by FastQC (version 0.11.5)