##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033040_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4518607 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.707239863966926 34.0 31.0 34.0 31.0 34.0 2 32.713642058271496 34.0 31.0 34.0 31.0 34.0 3 32.81866314109636 34.0 31.0 34.0 31.0 34.0 4 36.302864798819634 37.0 37.0 37.0 35.0 37.0 5 36.287525558208536 37.0 37.0 37.0 35.0 37.0 6 36.35140475814781 37.0 37.0 37.0 35.0 37.0 7 36.30096797530744 37.0 37.0 37.0 35.0 37.0 8 36.3136305060387 37.0 37.0 37.0 35.0 37.0 9 38.116790417931895 39.0 39.0 39.0 37.0 39.0 10-11 38.079355540324705 39.0 39.0 39.0 36.0 39.0 12-13 38.05128483180768 39.0 39.0 39.0 35.0 39.0 14-15 39.54758169940426 41.0 40.0 41.0 37.0 41.0 16-17 39.544683571729074 41.0 40.0 41.0 37.0 41.0 18-19 39.55689596373396 41.0 40.0 41.0 37.0 41.0 20-21 39.545851962784106 41.0 40.0 41.0 37.0 41.0 22-23 39.47447675799201 41.0 39.0 41.0 36.5 41.0 24-25 39.4196002219268 41.0 39.0 41.0 36.0 41.0 26-27 39.310292751726365 41.0 39.0 41.0 36.0 41.0 28-29 39.26026527644471 41.0 39.0 41.0 36.0 41.0 30-31 39.26163218000592 41.0 39.0 41.0 36.0 41.0 32-33 39.231177882918345 41.0 39.0 41.0 35.5 41.0 34-35 39.15523390283775 41.0 39.0 41.0 35.0 41.0 36-37 39.07199054929982 41.0 39.0 41.0 35.0 41.0 38-39 38.98199611074829 41.0 39.0 41.0 35.0 41.0 40-41 38.88514989243366 41.0 39.0 41.0 35.0 41.0 42-43 38.7746968258138 41.0 39.0 41.0 35.0 41.0 44-45 38.61585085846147 41.0 38.0 41.0 35.0 41.0 46-47 38.43103460867475 41.0 38.0 41.0 34.5 41.0 48-49 38.23900418868027 40.0 38.0 41.0 34.0 41.0 50-51 38.10222862930988 40.0 38.0 41.0 34.0 41.0 52-53 37.96406292912838 40.0 38.0 41.0 34.0 41.0 54-55 37.812732994925206 40.0 37.0 41.0 33.5 41.0 56-57 37.653532382878176 40.0 37.0 41.0 33.0 41.0 58-59 37.469164390707135 40.0 36.5 41.0 33.0 41.0 60-61 37.26571219847179 40.0 36.0 41.0 33.0 41.0 62-63 37.02641444586794 39.0 35.5 41.0 33.0 41.0 64-65 36.75353997371313 39.0 35.0 41.0 32.0 41.0 66-67 36.466972896735655 38.5 35.0 41.0 32.0 41.0 68-69 36.15185919023274 37.5 35.0 40.0 31.5 41.0 70-71 35.81992370657594 37.0 35.0 40.0 31.0 41.0 72-73 35.469870913757276 36.5 35.0 39.0 31.0 41.0 74-75 35.11764521676703 36.0 35.0 39.0 31.0 41.0 76-77 34.76191689164382 35.5 35.0 38.5 31.0 40.0 78-79 34.40492932888388 35.0 34.5 37.0 30.5 39.0 80-81 34.07984473976161 35.0 34.0 37.0 30.0 39.0 82-83 33.78623091142912 35.0 34.0 36.5 30.0 39.0 84-85 33.57243747907265 35.0 34.0 36.0 30.0 37.0 86-87 33.37317573756691 35.0 34.0 36.0 30.0 37.0 88-89 33.174362032369714 35.0 34.0 35.0 30.0 36.5 90-91 33.00227492676393 35.0 34.0 35.0 30.0 36.0 92-93 32.83793246458477 35.0 34.0 35.0 29.5 36.0 94-95 32.689475207735484 35.0 34.0 35.0 29.0 36.0 96-97 32.552306938841994 35.0 34.0 35.0 29.0 36.0 98-99 32.431186204066876 35.0 34.0 35.0 29.0 35.0 100 32.327356417586216 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0687192218455479 1101 2 0.1018970963544703 1101 3 0.162348926988102 1101 4 0.1247066371194876 1101 5 0.10959072563009897 1101 6 0.08809564871950926 1101 7 0.17993161261438217 1101 8 0.1642190952394742 1101 9 0.19965416158348148 1101 10-11 0.2192721703422933 1101 12-13 0.22424568765753605 1101 14-15 0.33168624552440207 1101 16-17 0.28606428251101335 1101 18-19 0.2696194933782863 1101 20-21 0.28195284054595504 1101 22-23 0.25914356063978516 1101 24-25 0.3273615981978253 1101 26-27 0.3445520421263879 1101 28-29 0.34658886605098616 1101 30-31 0.3590559581315915 1101 32-33 0.37003744724434995 1101 34-35 0.34942296133347384 1101 36-37 0.34718503700582914 1101 38-39 0.25010246731422114 1101 40-41 0.27038692650469187 1101 42-43 0.30287639930572396 1101 44-45 0.27527655049774324 1101 46-47 0.30568182919110853 1101 48-49 0.29971041412771626 1101 50-51 0.34809267732132554 1101 52-53 0.301114576762167 1101 54-55 0.31049010909607233 1101 56-57 0.2723242232568097 1101 58-59 0.29377269995371336 1101 60-61 0.24322785704688954 1101 62-63 0.2411960061496501 1101 64-65 0.2078695523326033 1101 66-67 0.21003063927744137 1101 68-69 0.2374720637198564 1101 70-71 0.22026418757383226 1101 72-73 0.21648853230440324 1101 74-75 0.15885401307306068 1101 76-77 0.17447659950161665 1101 78-79 0.20492657168278328 1101 80-81 0.260773615360705 1101 82-83 0.23644622670084203 1101 84-85 0.2874642681148316 1101 86-87 0.2535345623739005 1101 88-89 0.284172064381103 1101 90-91 0.2921510978872419 1101 92-93 0.3229768374215851 1101 94-95 0.296372176873831 1101 96-97 0.38122354824155025 1101 98-99 0.399253014703838 1101 100 0.37027154146766605 1102 1 0.13511953063050441 1102 2 0.0818967238675512 1102 3 0.12075306815038545 1102 4 0.10631844151969716 1102 5 0.11975989714113666 1102 6 0.1495540933435322 1102 7 0.16142146658389578 1102 8 0.1515614546518762 1102 9 0.20564205598187613 1102 10-11 0.20945354358778445 1102 12-13 0.24658985597344696 1102 14-15 0.348954052908077 1102 16-17 0.3336576824890187 1102 18-19 0.31794152905717965 1102 20-21 0.2990228897209164 1102 22-23 0.24598819277996142 1102 24-25 0.32348728821868633 1102 26-27 0.34868653532895166 1102 28-29 0.3700721305708967 1102 30-31 0.32082343864126983 1102 32-33 0.33518452158269696 1102 34-35 0.30740272949493885 1102 36-37 0.30260501711457266 1102 38-39 0.2826074707948507 1102 40-41 0.32545033403726364 1102 42-43 0.2929710355164872 1102 44-45 0.28692276086720625 1102 46-47 0.2945697583623854 1102 48-49 0.31193010378589037 1102 50-51 0.2575746495123994 1102 52-53 0.2667730120628633 1102 54-55 0.25109994266134095 1102 56-57 0.2467421789995683 1102 58-59 0.2665631165122946 1102 60-61 0.2571644994766302 1102 62-63 0.2254609712574478 1102 64-65 0.30613645102663867 1102 66-67 0.2768875790989469 1102 68-69 0.32622245154118445 1102 70-71 0.29579904086193665 1102 72-73 0.2720259405850811 1102 74-75 0.2626594241257507 1102 76-77 0.26295969450652024 1102 78-79 0.2815860510620709 1102 80-81 0.2415609578985567 1102 82-83 0.24620496558952 1102 84-85 0.32909497789867714 1102 86-87 0.33379899573989746 1102 88-89 0.3542891847494687 1102 90-91 0.3158032361517087 1102 92-93 0.3088672785375266 1102 94-95 0.3250652117477486 1102 96-97 0.30679221531163137 1102 98-99 0.29060237419363233 1102 100 0.32062282785233265 1103 1 0.09683843067059428 1103 2 0.01666533641069634 1103 3 0.07498814325018088 1103 4 0.06499416610522957 1103 5 0.12601246695523116 1103 6 0.12039167121131555 1103 7 0.1173244772352291 1103 8 0.13279601159981524 1103 9 0.19305716350268654 1103 10-11 0.156811049679078 1103 12-13 0.18285809064381198 1103 14-15 0.26346761637072547 1103 16-17 0.272673174628018 1103 18-19 0.2497341787336822 1103 20-21 0.2431171615650385 1103 22-23 0.2405042309977432 1103 24-25 0.19704609780990268 1103 26-27 0.19471699694382494 1103 28-29 0.2397929751841943 1103 30-31 0.2354455143163534 1103 32-33 0.2463736800929155 1103 34-35 0.31613703617467337 1103 36-37 0.29793751507483535 1103 38-39 0.38021558880802075 1103 40-41 0.3385612462466199 1103 42-43 0.36291103666793845 1103 44-45 0.3483180093373406 1103 46-47 0.3457508954006627 1103 48-49 0.3413031593737941 1103 50-51 0.410198942585275 1103 52-53 0.396366454047687 1103 54-55 0.34928365069522727 1103 56-57 0.3693694914026011 1103 58-59 0.3604913251977422 1103 60-61 0.36265931516537364 1103 62-63 0.28719091317373824 1103 64-65 0.29233979856260817 1103 66-67 0.28158295435732583 1103 68-69 0.26929588689228723 1103 70-71 0.3134826646854094 1103 72-73 0.3259492867633611 1103 74-75 0.3294543165490893 1103 76-77 0.3586265690497541 1103 78-79 0.32650790508331085 1103 80-81 0.3057055852094521 1103 82-83 0.34548348961290287 1103 84-85 0.3348167481821136 1103 86-87 0.3521599280351495 1103 88-89 0.3348721313665095 1103 90-91 0.30936503284971195 1103 92-93 0.3289045244615991 1103 94-95 0.32835352652113414 1103 96-97 0.4008406171826451 1103 98-99 0.4288869972424507 1103 100 0.39900532617778595 1104 1 0.07462353113250231 1104 2 0.08556859638387238 1104 3 0.07231255708470741 1104 4 0.026806210711910694 1104 5 0.10032593280555346 1104 6 0.09695885960529438 1104 7 0.08263273054148357 1104 8 0.09583721661985578 1104 9 0.11302546444922967 1104 10-11 0.13953608650980698 1104 12-13 0.14770516178747783 1104 14-15 0.16447679309520424 1104 16-17 0.15394103644320722 1104 18-19 0.18065921682098462 1104 20-21 0.17508711774107866 1104 22-23 0.19057331965274216 1104 24-25 0.17702054683858393 1104 26-27 0.14440133645010178 1104 28-29 0.1738958355269702 1104 30-31 0.1989814438623938 1104 32-33 0.18485663094534033 1104 34-35 0.21288824547314533 1104 36-37 0.20902615933304247 1104 38-39 0.2065651141177085 1104 40-41 0.2169156219239241 1104 42-43 0.22253967862378232 1104 44-45 0.272485033890554 1104 46-47 0.2599803867788637 1104 48-49 0.21086797912969502 1104 50-51 0.28296581667412823 1104 52-53 0.3038308510076675 1104 54-55 0.2700417172282812 1104 56-57 0.32982422299377134 1104 58-59 0.27892725441950716 1104 60-61 0.3495759187163259 1104 62-63 0.26436485353399775 1104 64-65 0.2521548311757158 1104 66-67 0.22563799099146564 1104 68-69 0.24675622938109143 1104 70-71 0.20154583184657326 1104 72-73 0.17826925294131257 1104 74-75 0.1922336605942263 1104 76-77 0.1603574183363179 1104 78-79 0.14056343218583578 1104 80-81 0.15062351940926533 1104 82-83 0.11511675453738235 1104 84-85 0.14114629602497786 1104 86-87 0.12041814606618573 1104 88-89 0.119483290252127 1104 90-91 0.1456586782033753 1104 92-93 0.18238028346650736 1104 94-95 0.21061905830743655 1104 96-97 0.24487466957236848 1104 98-99 0.26428962923368005 1104 100 0.2529323870418736 1105 1 0.015998139732104733 1105 2 0.038699741748281724 1105 3 0.06106239112072842 1105 4 0.006075502856504045 1105 5 0.005316515493774432 1105 6 0.02379095261680675 1105 7 0.022400306777477397 1105 8 0.037288062665211896 1105 9 0.05756068725720098 1105 10-11 0.06663179487876647 1105 12-13 0.0674517489995452 1105 14-15 0.10359754338150395 1105 16-17 0.09237900994639858 1105 18-19 0.12962737611326247 1105 20-21 0.09611875755771848 1105 22-23 0.10287969821942511 1105 24-25 0.10874219407229191 1105 26-27 0.07428260210828341 1105 28-29 0.09149663371496075 1105 30-31 0.07981415043479956 1105 32-33 0.11197106747041374 1105 34-35 0.11067217347300584 1105 36-37 0.1337835821383493 1105 38-39 0.07573036009415546 1105 40-41 0.10330170251275916 1105 42-43 0.07254668331547975 1105 44-45 0.06754691653586065 1105 46-47 0.061937988545849976 1105 48-49 0.044200192386817605 1105 50-51 0.01918421824009897 1105 52-53 0.08376561054247844 1105 54-55 0.07348939372290175 1105 56-57 0.01567521788159354 1105 58-59 0.021415481334521758 1105 60-61 0.018971223530250825 1105 62-63 0.03753929068726336 1105 64-65 0.08263722126516626 1105 66-67 0.09025736548694852 1105 68-69 0.08765574982467683 1105 70-71 0.0980881975130643 1105 72-73 0.13717034447846999 1105 74-75 0.12915823047050878 1105 76-77 0.0857507072057544 1105 78-79 0.12493983830302824 1105 80-81 0.08709297702645102 1105 82-83 0.1449579696423129 1105 84-85 0.1907677680739397 1105 86-87 0.10232720493431202 1105 88-89 0.1295735510521112 1105 90-91 0.1426635582670599 1105 92-93 0.15702223966203377 1105 94-95 0.09776391224853143 1105 96-97 0.12971499680612908 1105 98-99 0.06910839945647496 1105 100 0.06521093713428883 1106 1 0.027971110678215894 1106 2 0.028328636009895547 1106 3 0.03371542088724766 1106 4 0.032942469979396094 1106 5 0.032244599949571295 1106 6 0.06505150187945219 1106 7 0.07003005792857664 1106 8 0.08834617986899929 1106 9 0.0367612605363945 1106 10-11 0.1280847536041847 1106 12-13 0.10984771376862312 1106 14-15 0.09988257005517198 1106 16-17 0.15764168887218233 1106 18-19 0.15698472497957994 1106 20-21 0.12822713052611334 1106 22-23 0.14477043483590535 1106 24-25 0.12874282394793823 1106 26-27 0.12423671677557024 1106 28-29 0.1495465899653894 1106 30-31 0.15176058810011028 1106 32-33 0.17714362892346713 1106 34-35 0.10607328465862054 1106 36-37 0.12035259221950412 1106 38-39 0.08859702483979248 1106 40-41 0.08259383634489126 1106 42-43 0.10156871178475058 1106 44-45 0.14272374119418174 1106 46-47 0.05153484642688255 1106 48-49 0.07394769768210807 1106 50-51 0.07549056417260402 1106 52-53 0.1081725876990518 1106 54-55 0.1232344233852487 1106 56-57 0.05848122523285326 1106 58-59 0.07218981970186888 1106 60-61 0.04503584960300344 1106 62-63 0.060319281495885946 1106 64-65 0.05085733953031735 1106 66-67 0.05846561586048438 1106 68-69 0.12210245532567399 1106 70-71 0.10130404980223773 1106 72-73 0.11038521732024265 1106 74-75 0.14679282067359622 1106 76-77 0.11905644422768802 1106 78-79 0.14437921388014274 1106 80-81 0.13558958068523452 1106 82-83 0.12933051929211814 1106 84-85 0.15491341287172844 1106 86-87 0.11868914847565293 1106 88-89 0.1526343075457035 1106 90-91 0.10555133354441892 1106 92-93 0.1365412141579796 1106 94-95 0.16756265404884374 1106 96-97 0.12974112233018786 1106 98-99 0.12882317384553943 1106 100 0.062048582714524514 1107 1 -0.03728586352931984 1107 2 -0.020171171478310157 1107 3 0.004756185838253657 1107 4 0.027493792035713227 1107 5 0.04933605414944964 1107 6 0.055911544929259094 1107 7 0.03807174995868934 1107 8 -0.006803945589481941 1107 9 0.052128616213337864 1107 10-11 0.07764518039202528 1107 12-13 0.025923180074549634 1107 14-15 0.07751936157419692 1107 16-17 0.04626268951179213 1107 18-19 0.061847266954430324 1107 20-21 0.05605604994669733 1107 22-23 0.0737095745784444 1107 24-25 0.07198893903421322 1107 26-27 0.11947752335541395 1107 28-29 0.09017481677276606 1107 30-31 0.10359808836258821 1107 32-33 0.035755623847727236 1107 34-35 0.06218802952224678 1107 36-37 0.08136157972472802 1107 38-39 0.09709539012135338 1107 40-41 0.008689696001503933 1107 42-43 0.03332647160547708 1107 44-45 0.022105204475444395 1107 46-47 0.1216972150426443 1107 48-49 0.1192959252028416 1107 50-51 0.0772702555797693 1107 52-53 0.10282802610033315 1107 54-55 0.0972624125051027 1107 56-57 0.0993715561710431 1107 58-59 0.1369847036430727 1107 60-61 0.081549311278863 1107 62-63 0.0744265317970445 1107 64-65 0.10346490246815421 1107 66-67 0.07974252278879135 1107 68-69 0.06070357196711029 1107 70-71 0.07355815572671531 1107 72-73 0.08080880721863082 1107 74-75 0.12386521995605193 1107 76-77 0.08561768392178948 1107 78-79 0.1034204677387578 1107 80-81 0.1386884884692634 1107 82-83 0.11384214260809955 1107 84-85 0.0909484504833884 1107 86-87 0.09504386298345935 1107 88-89 0.08658475028963863 1107 90-91 0.09398474996356754 1107 92-93 0.1196933508287259 1107 94-95 0.1422317701803948 1107 96-97 0.13844015303146762 1107 98-99 0.11355990132378224 1107 100 0.08552448968781334 1108 1 -0.05051401754450069 1108 2 -0.004830911712716102 1108 3 0.018617868129865656 1108 4 -0.017917315521316368 1108 5 -0.026789753233337876 1108 6 -0.040634444467080755 1108 7 -0.005375099039333975 1108 8 -0.024988073421837953 1108 9 -0.022596010211771045 1108 10-11 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-0.04572819531247063 2214 52-53 -0.053522405214700086 2214 54-55 0.006852644217957504 2214 56-57 -0.047218728823551714 2214 58-59 -0.06365244058990527 2214 60-61 -0.06076953003526597 2214 62-63 -0.048839047811569 2214 64-65 -0.08599268305317764 2214 66-67 -0.06187697519952451 2214 68-69 -0.03859848647432784 2214 70-71 -0.04794766823344787 2214 72-73 -0.03690575581798328 2214 74-75 -0.08071795379486701 2214 76-77 -0.04710941454157336 2214 78-79 -0.03102508983567276 2214 80-81 -0.08867935481848122 2214 82-83 -0.05997335175428731 2214 84-85 -0.04084601704200708 2214 86-87 -0.058391904112525594 2214 88-89 -0.009562165199888284 2214 90-91 -0.012415600853742603 2214 92-93 -0.0543816163040205 2214 94-95 -0.0730804098591662 2214 96-97 -0.03384157280323308 2214 98-99 0.0059468760003369425 2214 100 0.019265229935037098 2215 1 -0.20090743088628926 2215 2 -0.14328222559160508 2215 3 -0.1831315819822663 2215 4 -0.13314572584667417 2215 5 -0.13792449864075707 2215 6 -0.15733893398071785 2215 7 -0.1919417115974511 2215 8 -0.18284306195965883 2215 9 -0.24102554073130023 2215 10-11 -0.23240338685714335 2215 12-13 -0.22954302905129254 2215 14-15 -0.3334206912873796 2215 16-17 -0.3375850681877992 2215 18-19 -0.3336125830782919 2215 20-21 -0.3454791467162508 2215 22-23 -0.3479008946444253 2215 24-25 -0.37797238919517895 2215 26-27 -0.41226760783390404 2215 28-29 -0.4147849060372053 2215 30-31 -0.44976592363310886 2215 32-33 -0.34632067854889925 2215 34-35 -0.3442051455276527 2215 36-37 -0.3335613872567933 2215 38-39 -0.33168243808957953 2215 40-41 -0.33687932948641475 2215 42-43 -0.3399630330692389 2215 44-45 -0.3288475811517273 2215 46-47 -0.3314986612936792 2215 48-49 -0.26026810336710327 2215 50-51 -0.26508217931406364 2215 52-53 -0.27287540390374687 2215 54-55 -0.31175871120173326 2215 56-57 -0.22811891216782954 2215 58-59 -0.25489442721828937 2215 60-61 -0.20561666409171409 2215 62-63 -0.21056966930000698 2215 64-65 -0.22773848611807068 2215 66-67 -0.22500777808399874 2215 68-69 -0.184879444379078 2215 70-71 -0.2071732071885961 2215 72-73 -0.1864961322762042 2215 74-75 -0.20922646333479378 2215 76-77 -0.1828535071337214 2215 78-79 -0.22158258281596233 2215 80-81 -0.2187525332357012 2215 82-83 -0.221121175793769 2215 84-85 -0.22314103720815126 2215 86-87 -0.20206770307896704 2215 88-89 -0.19710695306739723 2215 90-91 -0.18738103765676328 2215 92-93 -0.21028968252198865 2215 94-95 -0.19758655739590125 2215 96-97 -0.18470503280202877 2215 98-99 -0.18172299431058292 2215 100 -0.2251681833215713 2216 1 -0.22982716169797612 2216 2 -0.19676119315811746 2216 3 -0.18813152835385694 2216 4 -0.15145099350384328 2216 5 -0.19086998251648168 2216 6 -0.2160184853940308 2216 7 -0.17276584336394762 2216 8 -0.1931392131786751 2216 9 -0.24974820910862405 2216 10-11 -0.26146428081394646 2216 12-13 -0.2772687250675787 2216 14-15 -0.35335687879673827 2216 16-17 -0.364724685742452 2216 18-19 -0.30816610413646117 2216 20-21 -0.3236866669992011 2216 22-23 -0.3417428265913429 2216 24-25 -0.3635203720205311 2216 26-27 -0.3603041470140269 2216 28-29 -0.4029523077865207 2216 30-31 -0.3582831041743191 2216 32-33 -0.34428151765349213 2216 34-35 -0.36505240696263286 2216 36-37 -0.36586773834793007 2216 38-39 -0.35082346885792504 2216 40-41 -0.37061204233947365 2216 42-43 -0.35772397933544653 2216 44-45 -0.37401752623203066 2216 46-47 -0.30909513971882774 2216 48-49 -0.3188389977347228 2216 50-51 -0.399395029776791 2216 52-53 -0.3611579233122555 2216 54-55 -0.3513168629263461 2216 56-57 -0.37636315713199764 2216 58-59 -0.30350777848638444 2216 60-61 -0.2860093307716909 2216 62-63 -0.350802214420753 2216 64-65 -0.41855750204201314 2216 66-67 -0.4243156327029993 2216 68-69 -0.413857127482089 2216 70-71 -0.45064254768485057 2216 72-73 -0.37755884943101137 2216 74-75 -0.4493474865604341 2216 76-77 -0.4295974713398536 2216 78-79 -0.48930451196131486 2216 80-81 -0.4687086083449401 2216 82-83 -0.4048347280409885 2216 84-85 -0.5464022031268101 2216 86-87 -0.5035381325540556 2216 88-89 -0.6018927162387726 2216 90-91 -0.5948909701849345 2216 92-93 -0.607076515853251 2216 94-95 -0.4517703087260685 2216 96-97 -0.5523373674921714 2216 98-99 -0.6718614362688058 2216 100 -0.658858610941099 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30.0 3 10.0 4 26.0 5 36.0 6 96.0 7 254.0 8 603.0 9 1035.0 10 1799.0 11 3002.0 12 4419.0 13 6769.0 14 8012.0 15 10493.0 16 12561.0 17 17845.0 18 20975.0 19 11397.0 20 8142.0 21 8067.0 22 8361.0 23 9212.0 24 10750.0 25 13172.0 26 16670.0 27 22014.0 28 28573.0 29 36144.0 30 46763.0 31 59992.0 32 77549.0 33 100332.0 34 139970.0 35 213765.0 36 399261.0 37 900532.0 38 1603460.0 39 714706.0 40 1810.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43682706124338 19.802084325051975 13.749770290286655 24.011318323417992 2 15.56553555340959 20.670708210848012 39.76537391826355 23.998382317478846 3 17.798723904330448 25.93984246880028 30.426405398287475 25.8350282285818 4 12.281224749679485 17.20446598843379 38.981460026508756 31.53284923537797 5 12.670121867419526 37.97929402454167 35.91203822246674 13.438545885572054 6 30.357318322207476 38.53338331927294 17.86312763853615 13.246170719983427 7 26.79299173395695 32.84954854449612 22.979117236794437 17.37834248475249 8 25.225778295539847 35.76246760052689 20.70782225967735 18.30393184425591 9 25.49221474671287 16.10423300809298 21.098869629511928 37.304682615682225 10-11 23.93274428331941 26.447851369071003 29.192156819351233 20.427247528258356 12-13 24.923610307335867 23.953731758482206 29.21929258286901 21.903365351312914 14-15 22.139368305926034 25.603641662547577 26.603841835366765 25.653148196159627 16-17 21.38379067421841 28.98774565808787 27.126694591967315 22.5017690757264 18-19 21.304931936036105 27.702560571843303 29.56618787692285 21.426319615197738 20-21 22.469933764985537 26.989047730860417 29.15484794318249 21.386170560971557 22-23 22.07131763319717 26.87847922875261 29.127382761305377 21.92282037674484 24-25 21.900544367410102 26.969994229340294 29.133067650611178 21.99639375263843 26-27 21.65443942814146 27.025981498694286 29.146737927676714 22.17284114548754 28-29 21.766501405028222 27.032964357817175 29.231613605342815 21.968920631811788 30-31 22.00946952491153 27.376938943816786 28.83157074850941 21.782020782762274 32-33 21.750287201343248 27.06100574801039 29.280838098998206 21.90786895164815 34-35 21.885638649256286 26.905116997340112 29.19894339118228 22.010300962221322 36-37 22.20588114876996 27.159575948959493 28.66407722556974 21.970465676700808 38-39 22.023966678226277 27.39509100924245 28.71410370496925 21.86683860756202 40-41 21.920263214007775 27.086980062096604 28.906977806975583 22.085778916920034 42-43 21.95431025535082 27.03154976743939 28.774310312890677 22.239829664319114 44-45 22.018135235040358 27.218388321887698 28.625569782899905 22.137906660172042 46-47 22.141591424082687 27.398687250296383 28.346822372470097 22.11289895315083 48-49 22.198666535947915 27.348727163039406 28.12354559712761 22.32906070388507 50-51 22.284091092675244 27.337053211310476 27.97698494248338 22.4018707535309 52-53 22.224482014036624 27.367139917235555 28.013898973732392 22.394479094995425 54-55 22.24469842143829 27.68103090178013 27.831398039262982 22.242872637518598 56-57 22.074059549768325 27.396894662447963 27.92989078271246 22.599155005071253 58-59 22.002986761185472 27.058593500164985 28.2642969393001 22.674122799349444 60-61 22.28289603410963 27.44585886756693 27.915782452423944 22.3554626458995 62-63 22.109103535669288 27.401896203852203 27.94051352551793 22.548486734960573 64-65 22.11101784244569 27.19904607769607 28.02758682045152 22.662349259406717 66-67 21.971851277600738 27.24238103052346 27.972705744569705 22.8130619473061 68-69 22.32832329518357 27.317396226039286 27.878344928014126 22.475935550763012 70-71 22.284920994456918 27.210863879067155 27.851127570952727 22.653087555523197 72-73 22.128347088727438 27.355961183475113 27.88247486882526 22.633216858972187 74-75 22.274454477050416 27.376001416367902 27.691054751471693 22.65848935510999 76-77 22.24673444714267 27.332084865977503 27.669722106835138 22.75145858004469 78-79 22.282475550540244 27.435335712975263 27.71871950802537 22.563469228459123 80-81 22.233168319351517 27.286373875842713 27.789095179111616 22.691362625694158 82-83 22.07347164238131 27.34543582686719 27.851676641916008 22.72941588883549 84-85 22.094740702167726 27.291939750458493 27.703504642027955 22.90981490534583 86-87 22.219774307689875 27.345183575700847 27.68759161467676 22.747450501932516 88-89 21.929141503979462 27.338288872720835 27.69908238607285 23.03348723722685 90-91 21.892101181119177 27.25106788497361 27.816176612162725 23.040654321744487 92-93 22.00561526152528 27.386721288564537 27.642874855444028 22.964788594466157 94-95 21.824299987883368 27.18216387550742 27.75312833172793 23.24040780488129 96-97 21.850013317194097 27.152027029643843 27.745098614181913 23.252861038980154 98-99 21.798969105247 27.268097921501777 27.647330511072127 23.285602462179096 100 21.833033941655028 27.148344611514126 27.571351082313644 23.447270364517205 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 24.0 1 26.5 2 120.0 3 237.0 4 171.5 5 112.5 6 292.5 7 717.5 8 1247.5 9 1463.0 10 1169.5 11 903.5 12 945.5 13 1143.5 14 1416.5 15 1674.5 16 1938.0 17 2229.5 18 2559.5 19 3021.5 20 3669.5 21 4520.0 22 5735.0 23 7951.5 24 10829.5 25 14314.5 26 19525.5 27 26596.0 28 35830.0 29 46415.0 30 57257.5 31 69584.5 32 83307.0 33 97956.0 34 113178.5 35 126350.5 36 137270.5 37 147654.5 38 157031.0 39 162964.0 40 168436.0 41 172689.0 42 173526.5 43 174253.0 44 172604.5 45 168414.5 46 164616.0 47 159764.5 48 154939.5 49 151247.5 50 147632.0 51 142318.0 52 132888.5 53 125786.5 54 120918.5 55 113379.0 56 103042.0 57 92879.0 58 84334.0 59 76758.0 60 69193.0 61 59527.0 62 50440.5 63 42327.0 64 34430.5 65 27852.5 66 21885.5 67 16485.0 68 12870.0 69 10179.5 70 7316.0 71 4935.5 72 3605.5 73 2706.5 74 1923.0 75 1242.5 76 732.5 77 477.0 78 325.5 79 190.5 80 114.0 81 43.0 82 15.5 83 9.5 84 6.5 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04508026478071671 2 0.24175592168117296 3 0.003031907842394791 4 0.002213071417806417 5 1.7704571342451334E-4 6 6.639214253419251E-5 7 0.0 8 3.319607126709625E-4 9 0.0 10-11 7.081828536980533E-4 12-13 0.0 14-15 2.545032130477379E-4 16-17 9.958821380128876E-5 18-19 5.864639257187004E-4 20-21 0.0 22-23 1.1065357089032084E-4 24-25 0.001316777493594818 26-27 1.5491499924644918E-4 28-29 3.2089535558193046E-4 30-31 1.438496421574171E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.532678544516042E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1065357089032084E-5 68-69 2.6556857013677003E-4 70-71 0.0 72-73 3.540914268490267E-4 74-75 1.5491499924644918E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.438496421574171E-4 84-85 0.0 86-87 4.758103548283797E-4 88-89 0.0020913524898270638 90-91 0.0021245485610941603 92-93 6.307253540748288E-4 94-95 5.422024973625721E-4 96-97 2.7663392722580214E-4 98-99 1.7704571342451334E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4518607.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.87128672557441 #Duplication Level Percentage of deduplicated Percentage of total 1 70.27816620384945 37.15697075911299 2 15.774885121268778 16.680769486191984 3 5.8543291312893695 9.285777422588495 4 2.7934271754755704 5.907683565663214 5 1.5517780085601434 4.1022250012512105 6 0.9226525467161231 2.9269096407305755 7 0.5914745679028457 2.1890415029333607 8 0.3986226183515172 1.6860552600129828 9 0.2925682319927799 1.3921612992436176 >10 1.4410143256905033 13.24598752141429 >50 0.07315723368470425 2.642701969119304 >100 0.027380433780119864 2.315303288062475 >500 3.768933886471493E-4 0.13056505745429062 >1k 8.375402472252653E-5 0.0885636611105102 >5k 8.375402472252455E-5 0.24928456511080482 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6125 0.13555062434064302 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5134 0.11361908659018145 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.213071417806417E-5 0.0 0.0 0.0 3 0.0 2.213071417806417E-5 0.0 0.0 0.0 4 0.0 2.213071417806417E-5 0.0 6.639214253419251E-5 0.0 5 0.0 2.213071417806417E-5 0.0 8.852285671225668E-5 0.0 6 0.0 2.213071417806417E-5 0.0 1.1065357089032084E-4 0.0 7 0.0 2.213071417806417E-5 0.0 1.1065357089032084E-4 0.0 8 0.0 2.213071417806417E-5 0.0 1.1065357089032084E-4 0.0 9 0.0 2.213071417806417E-5 0.0 1.3278428506838502E-4 0.0 10-11 0.0 2.213071417806417E-5 0.0 1.3278428506838502E-4 0.0 12-13 0.0 2.213071417806417E-5 0.0 1.3278428506838502E-4 0.0 14-15 0.0 2.213071417806417E-5 0.0 1.5491499924644918E-4 0.0 16-17 0.0 4.426142835612834E-5 0.0 1.7704571342451336E-4 0.0 18-19 0.0 4.426142835612834E-5 0.0 1.9917642760257752E-4 0.0 20-21 0.0 4.426142835612834E-5 0.0 2.4343785595870584E-4 0.0 22-23 0.0 6.639214253419251E-5 0.0 2.6556857013677003E-4 0.0 24-25 0.0 6.639214253419251E-5 0.0 3.3196071267096257E-4 0.0 26-27 0.0 6.639214253419251E-5 0.0 4.6474499773934753E-4 0.0 28-29 0.0 6.639214253419251E-5 0.0 0.001183993208526433 0.0 30-31 0.0 6.639214253419251E-5 0.0 0.004470404263968962 0.0 32-33 0.0 6.639214253419251E-5 0.0 0.012160827440846261 0.0 34-35 0.0 6.639214253419251E-5 0.0 0.023513883814193177 0.0 36-37 0.0 7.745749962322459E-5 0.0 0.043022108362156745 0.0 38-39 0.0 8.852285671225668E-5 0.0 0.0788738653306207 0.0 40-41 0.0 8.852285671225668E-5 0.0 0.11961651013243682 0.0 42-43 0.0 8.852285671225668E-5 0.0 0.1601378477924723 0.0 44-45 0.0 1.1065357089032086E-4 0.0 0.2100647389781851 0.0 46-47 0.0 1.3278428506838502E-4 0.0 0.27114551010964216 0.0 48-49 0.0 1.3278428506838502E-4 0.0 0.34064701798585273 0.0 50-51 0.0 1.3278428506838502E-4 0.0 0.4175180536833586 0.0 52-53 0.0 1.3278428506838502E-4 0.0 0.49392434438312516 0.0 54-55 0.0 1.3278428506838502E-4 0.0 0.5785300646858644 0.0 56-57 0.0 1.3278428506838502E-4 0.0 0.6786604809845158 0.0 58-59 0.0 1.3278428506838502E-4 0.0 0.7917484304344238 0.0 60-61 0.0 1.3278428506838502E-4 0.0 0.9148505280499056 0.0 62-63 0.0 1.3278428506838502E-4 0.0 1.0415156706480557 0.0 64-65 0.0 1.3278428506838502E-4 0.0 1.175395426068255 0.0 66-67 0.0 1.3278428506838502E-4 0.0 1.3289604517498423 0.0 68-69 0.0 1.3278428506838502E-4 0.0 1.5046340608953157 0.0 70-71 0.0 1.3278428506838502E-4 0.0 1.698963862092897 0.0 72-73 0.0 1.3278428506838502E-4 0.0 1.900331230399103 0.0 74-75 0.0 1.3278428506838502E-4 0.0 2.106323917968524 0.0 76-77 0.0 1.3278428506838502E-4 0.0 2.332455555439984 0.0 78-79 0.0 1.3278428506838502E-4 0.0 2.5916615452505605 0.0 80-81 0.0 1.3278428506838502E-4 0.0 2.876649817078582 0.0 82-83 0.0 1.3278428506838502E-4 0.0 3.1741972692026543 0.0 84-85 0.0 1.3278428506838502E-4 0.0 3.4737364856027533 0.0 86-87 0.0 1.3278428506838502E-4 0.0 3.7905044629904747 0.0 88 0.0 1.3278428506838502E-4 0.0 4.049699387443963 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5670 0.0 51.5439 1 GTATCAA 10495 0.0 43.611942 1 ATCAACG 12770 0.0 35.00119 3 TCAACGC 12835 0.0 34.786934 4 CAACGCA 12965 0.0 34.40111 5 AACGCAG 13645 0.0 32.755253 6 TATCAAC 13810 0.0 32.71797 2 ACGCAGA 15725 0.0 28.183506 7 CGCAGAG 15920 0.0 27.720516 8 GTACATG 13325 0.0 26.000921 1 TACATGG 13295 0.0 24.984013 2 GCAGAGT 18115 0.0 24.050282 9 ACATGGG 14080 0.0 22.59848 3 AGAGTAC 16715 0.0 20.778627 10-11 GAGTACT 11225 0.0 20.076067 12-13 CAGAGTA 17765 0.0 19.629873 10-11 CATGGGG 10335 0.0 18.917826 4 AGTACTT 11795 0.0 18.866806 12-13 ACACCGT 705 0.0 18.665642 6 GAGTACA 10100 0.0 18.108356 1 >>END_MODULE