FastQCFastQC Report
Wed 25 May 2016
SRR1033039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868016
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18350.031.0012661
GTATCAA23450.028.8702011
GTACATG51950.028.2357391
TCAACGC22800.027.630484
CAACGCA22900.027.5098235
TACATGG51150.027.2979012
ATCAACG23650.026.6374193
AACGCAG24750.025.8272066
ACATGGG52550.025.5865523
GTACGAC1602.035018E-623.5065293
TATCAAC28700.022.4417722
ACGCAGA30100.021.0805057
CGCAGAG30700.020.6823658
CATGGGG36150.020.6779424
GAGTACA36100.019.5350341
GGGTACG1456.588489E-419.45421
ACAGCGT2753.180685E-818.8132928
CTGTCGC5800.017.8449769
GTATTAG8350.017.4544161
AGTACAT37850.017.1408232
Produced by FastQC (version 0.11.5)