##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033039_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1868016
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.397228931657974	33.0	31.0	34.0	30.0	34.0
2	31.442005314729638	33.0	31.0	34.0	28.0	34.0
3	31.616387118739883	34.0	31.0	34.0	30.0	34.0
4	34.99567723188667	37.0	35.0	37.0	32.0	37.0
5	35.02132904643215	37.0	35.0	37.0	33.0	37.0
6	35.18048881808293	37.0	35.0	37.0	33.0	37.0
7	35.03629572765972	37.0	35.0	37.0	32.0	37.0
8	35.053731338489605	37.0	35.0	37.0	33.0	37.0
9	36.68328376202345	39.0	37.0	39.0	33.0	39.0
10-11	36.68043876497846	39.0	37.5	39.0	33.0	39.0
12-13	36.61471261488124	39.0	37.0	39.0	33.0	39.0
14-15	37.98113265625133	41.0	38.0	41.0	33.0	41.0
16-17	37.976924448184604	40.5	38.0	41.0	33.0	41.0
18-19	37.97074650324194	40.0	38.5	41.0	33.0	41.0
20-21	38.01463451062518	40.0	38.5	41.0	33.0	41.0
22-23	37.963622634923894	40.0	38.0	41.0	33.0	41.0
24-25	37.97808664379748	40.0	38.0	41.0	33.0	41.0
26-27	37.90203295903247	40.0	38.0	41.0	33.0	41.0
28-29	37.915221015237556	40.0	38.0	41.0	33.0	41.0
30-31	38.05946603241085	40.0	38.0	41.0	33.0	41.0
32-33	38.05426184786426	40.0	38.0	41.0	33.0	41.0
34-35	38.013201974715415	40.0	38.0	41.0	33.0	41.0
36-37	37.930980783890504	40.0	38.0	41.0	33.0	41.0
38-39	37.83439354909166	40.0	38.0	41.0	32.5	41.0
40-41	37.760369290198796	40.0	38.0	41.0	32.0	41.0
42-43	37.631439987666056	40.0	38.0	41.0	32.0	41.0
44-45	37.47361318104342	40.0	38.0	41.0	31.5	41.0
46-47	37.340446227441305	40.0	38.0	41.0	31.0	41.0
48-49	37.224820344151226	40.0	37.0	41.0	31.0	41.0
50-51	37.099769755719436	40.0	37.0	41.0	31.0	41.0
52-53	36.94683102286062	40.0	37.0	41.0	30.0	41.0
54-55	36.795110427319685	40.0	36.5	41.0	30.0	41.0
56-57	36.62802540235201	40.0	36.0	41.0	30.0	41.0
58-59	36.43526019049088	39.5	35.5	41.0	30.0	41.0
60-61	36.21550725475585	39.0	35.0	41.0	30.0	41.0
62-63	35.96400432330344	39.0	35.0	41.0	29.5	41.0
64-65	35.68292857234627	38.5	35.0	40.5	29.0	41.0
66-67	35.382207647043714	38.0	35.0	40.0	29.0	41.0
68-69	35.05846068770289	37.0	34.5	40.0	28.5	41.0
70-71	34.71698850545177	37.0	34.0	39.5	28.0	41.0
72-73	34.35703334446814	36.0	34.0	39.0	28.0	41.0
74-75	34.00113864121079	36.0	34.0	39.0	28.0	40.5
76-77	33.63772365975452	35.0	34.0	38.5	27.0	40.0
78-79	33.251818774571525	35.0	34.0	37.0	26.5	39.0
80-81	32.90282470813955	35.0	33.0	37.0	26.0	39.0
82-83	32.680037001824395	35.0	33.0	36.5	26.0	39.0
84-85	32.45702740233489	35.0	33.0	36.0	26.0	37.0
86-87	32.2803546650564	35.0	33.0	36.0	26.0	37.0
88-89	32.058831937199685	35.0	33.0	35.0	26.0	37.0
90-91	31.850140202225248	35.0	33.0	35.0	26.0	36.0
92-93	31.63014636919598	35.0	33.0	35.0	25.0	36.0
94-95	31.433739325573228	35.0	33.0	35.0	25.0	36.0
96-97	31.26218378215176	35.0	33.0	35.0	25.0	36.0
98-99	31.0839203197403	35.0	33.0	35.0	24.0	35.0
100	30.942049211569923	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.006428717359334257
1101	2	0.15870984699085255
1101	3	0.090123448832518
1101	4	0.06129896837548188
1101	5	0.05722833298853658
1101	6	0.24473304029395848
1101	7	0.23990409534886936
1101	8	0.2569209505829875
1101	9	0.27879405097831267
1101	10-11	0.276002139570501
1101	12-13	0.25920346302057595
1101	14-15	0.2767192846556341
1101	16-17	0.33053847867440567
1101	18-19	0.2891141199075662
1101	20-21	0.3398525697366992
1101	22-23	0.3605734563659979
1101	24-25	0.28441967824918635
1101	26-27	0.17257939996246563
1101	28-29	0.15067508919100447
1101	30-31	0.21158693527736006
1101	32-33	0.1884614335556165
1101	34-35	0.15502745374690363
1101	36-37	0.21407506343057747
1101	38-39	0.21924519608755588
1101	40-41	0.24822808777304317
1101	42-43	0.2664032131257841
1101	44-45	0.27811295754664656
1101	46-47	0.22629102453352345
1101	48-49	0.20609176361211468
1101	50-51	0.21037027973779487
1101	52-53	0.05406697196156074
1101	54-55	0.08655101407484977
1101	56-57	0.24496156335050046
1101	58-59	0.19743919257648912
1101	60-61	0.20178435461485122
1101	62-63	0.11457967962589066
1101	64-65	0.12004346318560977
1101	66-67	0.10478471943754641
1101	68-69	0.037309704936355104
1101	70-71	0.014588604904133717
1101	72-73	0.09513879442479123
1101	74-75	-3.694888541545538E-4
1101	76-77	0.10955643353355526
1101	78-79	0.04825266179062737
1101	80-81	0.004733601155606948
1101	82-83	-0.0424649196321667
1101	84-85	-0.13528305862512013
1101	86-87	-0.11736417344687311
1101	88-89	-0.09410966589717162
1101	90-91	-0.13184868960141927
1101	92-93	-0.06996942154246355
1101	94-95	-0.08314890865440461
1101	96-97	-0.09530805019543465
1101	98-99	-0.11887374896499736
1101	100	-0.09029365277172374
1102	1	-0.17657982900207614
1102	2	-0.11568608709900019
1102	3	-0.03929698230286505
1102	4	-0.11712722658503338
1102	5	-0.1109993302173109
1102	6	0.053788902391204374
1102	7	0.10643100424131546
1102	8	0.04657855981015757
1102	9	0.1740962337865355
1102	10-11	0.12213466653617644
1102	12-13	0.13779638932122396
1102	14-15	0.25922905665802176
1102	16-17	0.24440478443160885
1102	18-19	0.10473307648801011
1102	20-21	0.2096216697029476
1102	22-23	0.19720008858222116
1102	24-25	0.16439803630040473
1102	26-27	0.1202459499014168
1102	28-29	0.11406580839145164
1102	30-31	0.17754045871912183
1102	32-33	0.01946920383548445
1102	34-35	0.11630051141354869
1102	36-37	0.12654095212281646
1102	38-39	0.07307907046961049
1102	40-41	0.04805425812609343
1102	42-43	0.1275371439788131
1102	44-45	0.12572524487348602
1102	46-47	0.09726352411551886
1102	48-49	-0.12284377429757853
1102	50-51	0.016120381480192236
1102	52-53	-0.025447787784415254
1102	54-55	-0.001225386105609516
1102	56-57	-0.012859035620408577
1102	58-59	-0.016322531460268408
1102	60-61	-0.10456437100246774
1102	62-63	-0.0023537256369507986
1102	64-65	-0.036981021240706013
1102	66-67	6.860438818989678E-5
1102	68-69	0.040891037335867964
1102	70-71	-0.08782150687054013
1102	72-73	-0.10942257753595186
1102	74-75	-0.13715196834004928
1102	76-77	-0.16308574555199584
1102	78-79	-0.21205786547159988
1102	80-81	-0.059585103356077695
1102	82-83	-0.04199548494131733
1102	84-85	-0.09017994388808859
1102	86-87	-0.1207456490744434
1102	88-89	-0.1392011078629949
1102	90-91	-0.033763497300398626
1102	92-93	0.0526733885036883
1102	94-95	0.06747053692051352
1102	96-97	0.040049720242169684
1102	98-99	-0.06362936214829418
1102	100	-0.09168618282911112
1103	1	-0.21964608877765812
1103	2	-0.16443156658973734
1103	3	-0.2149669588812877
1103	4	-0.18436109121773114
1103	5	-0.2860146851670393
1103	6	-0.1831415918752768
1103	7	-0.1292379104623933
1103	8	-0.18959414204559266
1103	9	-0.09143247725442905
1103	10-11	-0.12221839232667264
1103	12-13	-0.1751329610165797
1103	14-15	-0.1699591340884652
1103	16-17	-0.15363276852637142
1103	18-19	-0.13904922471517267
1103	20-21	-0.11633253936329169
1103	22-23	-0.19572824814790124
1103	24-25	-0.11813107777027909
1103	26-27	-0.1450875813267487
1103	28-29	-0.24741866832754766
1103	30-31	-0.19700688701642122
1103	32-33	-0.22439228546794254
1103	34-35	-0.22653705312852424
1103	36-37	-0.1826514740557812
1103	38-39	-0.1586201824773994
1103	40-41	-0.0961956902885106
1103	42-43	-0.14007338450899454
1103	44-45	-0.06598416828386178
1103	46-47	-0.08215242669072609
1103	48-49	-0.11796714483272552
1103	50-51	-0.19393125821123647
1103	52-53	-0.033269007902589465
1103	54-55	-0.06197442408331
1103	56-57	-0.01641435506972755
1103	58-59	-0.09222545680443517
1103	60-61	-0.18581905441296698
1103	62-63	-0.2111226689388701
1103	64-65	-0.23564842880632852
1103	66-67	-0.3012199443790067
1103	68-69	-0.32463716086787997
1103	70-71	-0.21044239467019565
1103	72-73	-0.31752106158912596
1103	74-75	-0.3072208574622266
1103	76-77	-0.34506527912353135
1103	78-79	-0.3344865901480034
1103	80-81	-0.1941439366453679
1103	82-83	-0.26689813637008086
1103	84-85	-0.31252474426167254
1103	86-87	-0.29097381940397327
1103	88-89	-0.3353081001873264
1103	90-91	-0.3019877712737298
1103	92-93	-0.33233131149860284
1103	94-95	-0.3355738156556818
1103	96-97	-0.3119474187294742
1103	98-99	-0.3090320781342122
1103	100	-0.36212938278856655
1104	1	0.029710118046637035
1104	2	0.007003585643321486
1104	3	0.06243343284975822
1104	4	0.0432425261465923
1104	5	0.016940009457655947
1104	6	0.029859566692671535
1104	7	0.1510770849397005
1104	8	0.10187098748369294
1104	9	0.14365507342209582
1104	10-11	0.12541987816863553
1104	12-13	0.16844131635046722
1104	14-15	0.19198576206471074
1104	16-17	0.13947388206927513
1104	18-19	0.1701152382478952
1104	20-21	0.09488487483081087
1104	22-23	0.19412683561014887
1104	24-25	0.19531285637318518
1104	26-27	0.05418134324857249
1104	28-29	0.09995948721149261
1104	30-31	0.13625154637110626
1104	32-33	0.08037927402686051
1104	34-35	0.06168248424091871
1104	36-37	0.1272228066016794
1104	38-39	0.12296854495087928
1104	40-41	0.1232490283561205
1104	42-43	0.07307454331689911
1104	44-45	0.05552995360302759
1104	46-47	0.0715446432926683
1104	48-49	0.13609022419628047
1104	50-51	0.10619823643493476
1104	52-53	-0.027813650867969386
1104	54-55	0.033510667796839755
1104	56-57	0.06911250760100529
1104	58-59	0.18403065425183485
1104	60-61	0.2582560135165224
1104	62-63	0.17641982487430852
1104	64-65	0.1409632188712564
1104	66-67	0.03963845229456098
1104	68-69	0.11678566967467674
1104	70-71	0.10422743078261476
1104	72-73	0.10120816774671226
1104	74-75	0.09477840825853434
1104	76-77	0.14798093197828877
1104	78-79	0.25260083237601805
1104	80-81	0.13774161970103904
1104	82-83	0.12800251945903796
1104	84-85	0.042448612139104114
1104	86-87	0.004631588631880845
1104	88-89	0.08782528925602406
1104	90-91	0.06786448100660536
1104	92-93	0.04820587264442722
1104	94-95	0.08024016058761418
1104	96-97	0.11863656705598657
1104	98-99	0.1778160542161551
1104	100	0.22075844739896766
1105	1	-0.14816396529790055
1105	2	-0.15367436877127716
1105	3	-0.11463761376650083
1105	4	-0.07298790388419718
1105	5	-0.12562417797273895
1105	6	-0.03618954914637129
1105	7	-0.07517641015940058
1105	8	0.017373402592859577
1105	9	0.04541429341595915
1105	10-11	-0.012195421315247756
1105	12-13	0.05100184922594764
1105	14-15	-0.01570914465794715
1105	16-17	0.006143651659932914
1105	18-19	0.00641082968244433
1105	20-21	0.032143200148262
1105	22-23	0.09792653841898868
1105	24-25	0.045499962689703466
1105	26-27	0.11195930490332984
1105	28-29	0.13634547716491596
1105	30-31	0.12035595631564178
1105	32-33	0.08089760024070358
1105	34-35	0.046926425781833814
1105	36-37	0.05264954484245976
1105	38-39	0.09867078836492027
1105	40-41	0.15039713091810114
1105	42-43	0.09083009496507088
1105	44-45	0.07901012419489462
1105	46-47	0.05766753976877936
1105	48-49	0.06118074338441204
1105	50-51	-0.1015507990728608
1105	52-53	0.017199502082050344
1105	54-55	-0.01650598456189556
1105	56-57	-0.07230346004583765
1105	58-59	-0.030511509313633667
1105	60-61	-0.10253088800701704
1105	62-63	-0.17054761855766287
1105	64-65	-0.07086623063554498
1105	66-67	-0.06385681919405073
1105	68-69	0.06285850564377427
1105	70-71	-0.04990349192354415
1105	72-73	0.03461707451763374
1105	74-75	-0.08761277392102329
1105	76-77	-0.004962191424993989
1105	78-79	0.006385744580498454
1105	80-81	-0.05280259868964521
1105	82-83	-0.010519523368209605
1105	84-85	0.0023194615473087765
1105	86-87	-0.0984367205158847
1105	88-89	-0.07927454559946767
1105	90-91	-0.059667487729711866
1105	92-93	0.03809034044124715
1105	94-95	0.05581649424158286
1105	96-97	0.02503865773721614
1105	98-99	-0.007594135096685051
1105	100	0.00992151984534928
1106	1	-0.12349708999936482
1106	2	-0.11465867196828583
1106	3	-0.01737080105752753
1106	4	0.0104874638294703
1106	5	-0.00954360214878136
1106	6	0.003636517758572211
1106	7	0.06948453560180212
1106	8	0.05597930248250549
1106	9	0.00862612613711633
1106	10-11	0.03541645752454059
1106	12-13	0.08521198544060127
1106	14-15	0.20631414524701341
1106	16-17	0.14628332520595677
1106	18-19	0.16328332815887592
1106	20-21	0.12060069689524511
1106	22-23	0.09591049572631505
1106	24-25	0.1085434624344046
1106	26-27	0.16616054681198023
1106	28-29	0.11972399576884385
1106	30-31	0.15592659192909508
1106	32-33	0.0970822723867073
1106	34-35	0.12110757314730591
1106	36-37	0.0921896762805332
1106	38-39	0.12496466282343732
1106	40-41	0.1441273040276485
1106	42-43	0.09649016776661057
1106	44-45	0.13065310016379073
1106	46-47	-0.04690841595734696
1106	48-49	-0.018745466817684076
1106	50-51	-0.017962440731153606
1106	52-53	-0.018994393788688058
1106	54-55	0.03649189068722336
1106	56-57	0.1075973849132339
1106	58-59	-0.04170878172646297
1106	60-61	3.459258549227684E-4
1106	62-63	0.008442383583556534
1106	64-65	0.04308860605510745
1106	66-67	0.011356067667087189
1106	68-69	-0.01646157407556359
1106	70-71	-0.03388319972911802
1106	72-73	-0.045297276609794324
1106	74-75	0.07662322166476088
1106	76-77	-0.11693985683437802
1106	78-79	-0.07621468695803912
1106	80-81	0.02276803692381435
1106	82-83	0.11018049145562969
1106	84-85	0.14546903412649925
1106	86-87	0.15651230193400067
1106	88-89	0.1404227185778879
1106	90-91	0.20385498686386683
1106	92-93	0.1899359089356416
1106	94-95	0.15728932543094842
1106	96-97	0.04358756818473353
1106	98-99	0.02221628441808221
1106	100	0.06266936627033104
1107	1	-0.0957619018063518
1107	2	-0.08131796634508959
1107	3	-0.07651599989072011
1107	4	-0.0534813672274268
1107	5	-0.04002166240426419
1107	6	-0.010828421933354093
1107	7	-0.01002098442663879
1107	8	-0.035527629476753475
1107	9	0.009050339102927296
1107	10-11	1.8662902851218632E-4
1107	12-13	0.03208035711300994
1107	14-15	0.11002426596456161
1107	16-17	0.07155847110441726
1107	18-19	0.06299460478388141
1107	20-21	0.01701345471679616
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2216	40-41	-0.7424699397149439
2216	42-43	-0.8093345453259957
2216	44-45	-0.6835479514244724
2216	46-47	-0.6633517118900585
2216	48-49	-0.6611383551510954
2216	50-51	-0.5916174142621671
2216	52-53	-0.6827719672474828
2216	54-55	-0.6580785741783117
2216	56-57	-0.8188626350121382
2216	58-59	-0.7165021648646857
2216	60-61	-0.670532301453072
2216	62-63	-0.6511944010047586
2216	64-65	-0.6207278961461569
2216	66-67	-0.5753881833003689
2216	68-69	-0.6755961266639403
2216	70-71	-0.6547495179152492
2216	72-73	-0.7551972143750305
2216	74-75	-0.7286500862795151
2216	76-77	-0.6596416140061052
2216	78-79	-0.747123135263493
2216	80-81	-0.6767584154128059
2216	82-83	-0.8561845628269111
2216	84-85	-0.6755035697709886
2216	86-87	-0.6520945356947223
2216	88-89	-0.7618310507232096
2216	90-91	-0.7206286310505234
2216	92-93	-0.7888515119583381
2216	94-95	-0.806287969892221
2216	96-97	-0.747981797968265
2216	98-99	-0.826200058482673
2216	100	-0.8138343204352481
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24432.0
3	4544.0
4	2565.0
5	2162.0
6	1786.0
7	1916.0
8	2301.0
9	2553.0
10	2819.0
11	3110.0
12	3550.0
13	3989.0
14	4575.0
15	4855.0
16	4715.0
17	4790.0
18	4563.0
19	4514.0
20	4343.0
21	4316.0
22	5120.0
23	7290.0
24	10814.0
25	14380.0
26	14646.0
27	12922.0
28	14443.0
29	17978.0
30	22107.0
31	26306.0
32	34325.0
33	46195.0
34	65666.0
35	100636.0
36	181269.0
37	377338.0
38	588320.0
39	235328.0
40	535.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29823847186028	19.673406201371833	13.231468065104496	23.796887261663393
2	15.466105552693335	19.87138222336949	40.82698616887617	23.835526055061003
3	18.44605904140075	24.989515237052053	29.495526233332175	27.06889948821502
4	12.061052589849318	16.609675177978982	39.225608616063084	32.10366361610862
5	12.645425934438984	37.9285088945968	35.75728643269928	13.66877873826493
6	30.98613433604317	38.22136519916053	17.872181285023807	12.920319179772493
7	27.423521122609994	32.11547580457199	22.433446833818678	18.02755623899934
8	24.783804841871287	37.03445297185953	20.23623118976196	17.945510996507224
9	24.493807050023175	17.42545820863932	21.310392313936884	36.77034242740062
10-11	23.607815167185965	27.257567155313332	28.84027220887173	20.29434546862897
12-13	24.374914927982182	24.42609211035408	28.949699528312507	22.249293433351234
14-15	21.629964268919586	26.66015549115833	25.821285649020847	25.88859459090124
16-17	21.294950038250793	29.980156731818013	26.417073996532785	22.30781923339841
18-19	21.12595226574544	28.894141968173948	28.446951490375934	21.53295427570468
20-21	22.45796971923017	28.257068808192322	27.856041767465932	21.428919705111575
22-23	22.005777300234282	28.076307926995835	27.927536821852826	21.99037795091705
24-25	21.890467348166993	28.150305946957772	27.97556707809411	21.983659626781122
26-27	21.658502758888655	27.961162913736093	28.16056123279432	22.21977309458093
28-29	21.688605054908912	28.04896993227517	28.132882727248866	22.12954228556705
30-31	22.02713171234513	28.616740187228316	27.677812790970524	21.678315309456025
32-33	21.434349516622756	28.229970353433785	28.382997849632872	21.952682280310587
34-35	21.59302952535514	27.90473477618118	28.416983062663277	22.08525263580041
36-37	22.09114833490488	28.394121117449373	27.671726951876902	21.843003595768845
38-39	21.89534447658845	28.467748848173148	27.91503267973856	21.721873995499838
40-41	21.56956503569787	28.184225217492866	28.214549386069525	22.03166036073974
42-43	21.558091456361964	28.061032338181857	28.16241896436173	22.21845724109445
44-45	21.746640884632	28.268372445222774	28.12126591968766	21.863720750457567
46-47	21.70942785574551	28.56397784466892	27.935518418085575	21.7910758815
48-49	21.698773522310955	28.258595616481365	28.019364563106663	22.023266298101017
50-51	21.68687349198798	28.10996005777052	28.04018436853135	22.162982081710144
52-53	21.677436981936673	28.063526332918592	28.176323342498705	22.08271334264603
54-55	21.792381246747443	28.59240792339196	27.86707638707168	21.74813444278891
56-57	21.599518738735505	28.291294844038728	27.9057608591135	22.203425558112272
58-59	21.436051709517773	27.82089046947927	28.45279628971943	22.290261531283527
60-61	21.809410709780472	28.358329677487436	27.857572529036524	21.97468708369557
62-63	21.626243776930252	28.42262474732067	27.881307742600953	22.069823733148127
64-65	21.580847022372783	28.007089052946665	28.183324387649172	22.228739537031384
66-67	21.449329163573093	28.18696853502352	27.927188829499443	22.436513471903936
68-69	22.01516892889963	28.276336240572984	27.868976280770624	21.839518549756757
70-71	21.963383113037988	28.06362653464212	27.94693735770388	22.026052994616013
72-73	21.648819413868793	28.261301563937835	28.129375442565806	21.960503579627563
74-75	21.74112512443667	28.277742916316157	28.004537971362893	21.97659398788428
76-77	21.73264835603037	28.281746236851713	27.81758422041965	22.168021186698272
78-79	21.916129531571062	28.413917657049247	27.895836362794018	21.774116448585666
80-81	21.81038155535079	28.08407194460757	28.071004155182145	22.0345423448595
82-83	21.590129730595375	28.20919396602629	28.21013126777024	21.990545035608093
84-85	21.695608014658962	28.156567921816723	27.983533033945314	22.164291029579005
86-87	21.84351399723022	28.371582896784552	27.857337179175474	21.927565926809752
88-89	21.580404191925425	28.217548044417036	27.988522198208976	22.21352556544856
90-91	21.547422145713224	28.089423416519466	28.061602774245532	22.30155166352178
92-93	21.799076137643763	28.386475546245464	27.815668814812216	21.998779501298554
94-95	21.58658677555165	28.04214018004152	28.094810049743167	22.276462994663664
96-97	21.590870750186504	28.10731838462235	28.08661926308985	22.215191602101296
98-99	21.58089945317527	28.171983754584655	28.03638214696654	22.21073464527353
100	21.735118094379096	28.09954227649156	27.91765261645812	22.247687012671218
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	321.0
1	302.0
2	264.5
3	228.0
4	223.0
5	236.5
6	231.5
7	202.5
8	187.5
9	185.0
10	176.0
11	172.0
12	164.5
13	179.0
14	210.0
15	246.5
16	328.5
17	455.5
18	617.5
19	840.5
20	1172.0
21	1796.0
22	2758.0
23	4131.0
24	5888.5
25	8033.5
26	10998.0
27	14610.0
28	19285.5
29	24734.5
30	30082.0
31	35965.0
32	42060.5
33	47532.5
34	52632.0
35	56675.5
36	60118.5
37	63879.5
38	66263.0
39	67403.5
40	68361.5
41	68557.0
42	67876.5
43	67286.5
44	66243.5
45	65068.0
46	64295.0
47	62881.0
48	61598.0
49	60326.5
50	58767.0
51	56447.5
52	52697.5
53	49737.0
54	47850.0
55	45533.0
56	41905.0
57	38041.5
58	34668.5
59	30756.0
60	26668.5
61	22549.0
62	18916.5
63	15713.5
64	12586.5
65	10000.5
66	7905.0
67	6032.5
68	4591.5
69	3491.5
70	2546.5
71	1854.5
72	1347.5
73	967.0
74	688.5
75	469.5
76	323.5
77	235.5
78	164.0
79	110.5
80	78.5
81	56.0
82	39.0
83	26.5
84	20.0
85	22.5
86	17.5
87	12.0
88	11.5
89	10.0
90	7.5
91	4.0
92	3.5
93	3.0
94	2.5
95	2.0
96	0.5
97	0.5
98	1.0
99	0.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06268682923486736
2	0.041648465537768416
3	0.05465691942681433
4	0.07296511378917525
5	0.08575943675000643
6	0.08602710041027485
7	0.08838254062063708
8	0.10053447079682401
9	0.09534179578761638
10-11	0.1004006389666898
12-13	0.10438882750468947
14-15	0.10671750134902484
16-17	0.07430343209051743
18-19	0.09684071228511962
20-21	0.09009558804635506
22-23	0.05642349958458601
24-25	0.01991417632397153
26-27	0.18235925174088444
28-29	0.06407868026826323
30-31	0.07590941405212803
32-33	0.07269745012890683
34-35	0.019646512663703094
36-37	0.05141818913756627
38-39	0.07580234858802065
40-41	0.08126268725749672
42-43	0.07489229214310798
44-45	0.07181416005002099
46-47	0.07855928428878553
48-49	0.07550791856172538
50-51	0.07066320631086671
52-53	0.07433019845654426
54-55	0.06501550307920274
56-57	0.06715681236135022
58-59	0.06439987666058535
60-61	0.06118791273736413
62-63	0.0681204015383166
64-65	0.06391808207210217
66-67	0.06648765321067913
68-69	0.06635382138054492
70-71	0.05837744430454557
72-73	0.056851761441015496
74-75	0.059019837089189814
76-77	0.053291834759445315
78-79	0.05002633810417042
80-81	0.04440540123853329
82-83	0.05109699274524416
84-85	0.04081870819093627
86-87	0.0384365016145472
88-89	0.040658109994775204
90-91	0.03733908060744662
92-93	0.03996218447807728
94-95	0.03878446437289616
96-97	0.042130260126251595
98-99	0.04183583009995632
100	0.05085609545100256
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1868016.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.38562973319691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.66931833830652	61.49409304209055
2	11.572733794010139	17.216901820041866
3	2.9744981214961284	6.6377974772310235
4	1.150099586432688	3.422035279707393
5	0.519376945347416	1.9317090574285865
6	0.30704510185055395	1.3703845954588225
7	0.19161890922171493	0.9977585261871891
8	0.12852632963314417	0.7648409573646302
9	0.08811732401732167	0.5899196373689366
>10	0.38390726316636653	4.7378609700012335
>50	0.012810093331609812	0.6358482315773776
>100	0.0018761241618587947	0.17318816270448023
>500	7.206902454171403E-5	0.027662242837929923
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.3532732053686904E-5	0.0
6	0.0	0.0	0.0	5.3532732053686904E-5	0.0
7	0.0	0.0	0.0	5.3532732053686904E-5	0.0
8	0.0	0.0	0.0	1.0706546410737381E-4	0.0
9	0.0	0.0	0.0	2.1413092821474762E-4	0.0
10-11	0.0	0.0	0.0	2.9443002629527795E-4	0.0
12-13	0.0	0.0	0.0	3.2119639232212145E-4	0.0
14-15	0.0	0.0	0.0	3.479627583489649E-4	5.3532732053686904E-5
16-17	0.0	0.0	0.0	3.7472912437580834E-4	5.3532732053686904E-5
18-19	0.0	0.0	0.0	3.7472912437580834E-4	5.3532732053686904E-5
20-21	0.0	0.0	0.0	3.7472912437580834E-4	5.3532732053686904E-5
22-23	0.0	0.0	0.0	4.014954904026518E-4	5.3532732053686904E-5
24-25	0.0	0.0	0.0	5.085609545100256E-4	5.3532732053686904E-5
26-27	0.0	0.0	0.0	7.226918827247732E-4	5.3532732053686904E-5
28-29	0.0	0.0	0.0	0.001070654641073738	1.0706546410737381E-4
30-31	0.0	0.0	0.0	0.0028640011648722492	1.6059819616106073E-4
32-33	0.0	0.0	0.0	0.007119853363140359	1.6059819616106073E-4
34-35	0.0	0.0	0.0	0.013597313941636473	1.6059819616106073E-4
36-37	0.0	0.0	0.0	0.025133617699206003	1.8736456218790417E-4
38-39	0.0	0.0	0.0	0.04611844866425127	2.1413092821474762E-4
40-41	0.0	0.0	0.0	0.07532055399953747	2.1413092821474762E-4
42-43	0.0	0.0	0.0	0.10776138962407175	2.1413092821474762E-4
44-45	0.0	0.0	0.0	0.14306622641347827	2.1413092821474762E-4
46-47	0.0	0.0	0.0	0.18342990638195816	2.1413092821474762E-4
48-49	0.0	0.0	0.0	0.23096697244563216	2.1413092821474762E-4
50-51	0.0	0.0	0.0	0.28350934895632585	2.1413092821474762E-4
52-53	0.0	0.0	0.0	0.33851423114148915	2.1413092821474762E-4
54-55	0.0	0.0	0.0	0.399407713852558	2.1413092821474762E-4
56-57	0.0	0.0	0.0	0.46691248897225723	2.1413092821474762E-4
58-59	0.0	0.0	0.0	0.5485766717201566	2.1413092821474762E-4
60-61	0.0	0.0	0.0	0.6409474008787933	2.1413092821474762E-4
62-63	0.0	0.0	0.0	0.7312303534873363	2.1413092821474762E-4
64-65	0.0	0.0	0.0	0.828579626726966	2.1413092821474762E-4
66-67	0.0	5.3532732053686904E-5	0.0	0.9394994475422052	2.1413092821474762E-4
68-69	0.0	5.3532732053686904E-5	0.0	1.0690218927461008	2.1413092821474762E-4
70-71	0.0	5.3532732053686904E-5	0.0	1.2123290164538205	3.2119639232212145E-4
72-73	0.0	5.3532732053686904E-5	0.0	1.367493640311432	3.2119639232212145E-4
74-75	0.0	5.3532732053686904E-5	0.0	1.5241571806665468	3.2119639232212145E-4
76-77	0.0	5.3532732053686904E-5	0.0	1.6852104050500638	3.2119639232212145E-4
78-79	0.0	5.3532732053686904E-5	0.0	1.874100650101498	3.2119639232212145E-4
80-81	0.0	5.3532732053686904E-5	0.0	2.0875891855316016	3.2119639232212145E-4
82-83	0.0	5.3532732053686904E-5	0.0	2.3188773543695556	3.2119639232212145E-4
84-85	0.0	5.3532732053686904E-5	0.0	2.5398872386532023	3.7472912437580834E-4
86-87	0.0	5.3532732053686904E-5	0.0	2.772058697570042	3.7472912437580834E-4
88	0.0	5.3532732053686904E-5	0.0	2.9664628140230063	3.7472912437580834E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1835	0.0	31.001266	1
GTATCAA	2345	0.0	28.870201	1
GTACATG	5195	0.0	28.235739	1
TCAACGC	2280	0.0	27.63048	4
CAACGCA	2290	0.0	27.509823	5
TACATGG	5115	0.0	27.297901	2
ATCAACG	2365	0.0	26.637419	3
AACGCAG	2475	0.0	25.827206	6
ACATGGG	5255	0.0	25.586552	3
GTACGAC	160	2.035018E-6	23.506529	3
TATCAAC	2870	0.0	22.441772	2
ACGCAGA	3010	0.0	21.080505	7
CGCAGAG	3070	0.0	20.682365	8
CATGGGG	3615	0.0	20.677942	4
GAGTACA	3610	0.0	19.535034	1
GGGTACG	145	6.588489E-4	19.4542	1
ACAGCGT	275	3.180685E-8	18.813292	8
CTGTCGC	580	0.0	17.844976	9
GTATTAG	835	0.0	17.454416	1
AGTACAT	3785	0.0	17.140823	2
>>END_MODULE