FastQCFastQC Report
Wed 25 May 2016
SRR1033039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868016
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35340.18918467507772954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29940.1602769997687386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27550.053.8796461
GTATCAA50950.041.990151
TCAACGC57900.036.4470864
ATCAACG59150.035.678773
CAACGCA59800.035.2871785
AACGCAG61350.034.3190466
TATCAAC63150.033.4896552
ACGCAGA70750.029.560067
CGCAGAG72300.028.926348
GCAGAGT81850.025.0345389
GTACATG47600.024.3536431
GTGGTAT13950.022.9705031
TACATGG47300.022.704472
GAGTACT58250.022.10682712-13
CAGAGTA80950.021.3649910-11
ACATGGG49650.020.9214273
GAGTACA36600.020.7291071
GGGTACG1405.120943E-420.1957041
AGAGTAC75000.020.05212810-11
GTACTTT64800.019.9447914-15
Produced by FastQC (version 0.11.5)