##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033039_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1868016 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68631264400305 34.0 31.0 34.0 31.0 34.0 2 32.689344202619246 34.0 31.0 34.0 31.0 34.0 3 32.79626031040419 34.0 31.0 34.0 31.0 34.0 4 36.28887654067203 37.0 37.0 37.0 35.0 37.0 5 36.27215452116042 37.0 37.0 37.0 35.0 37.0 6 36.34529522231073 37.0 37.0 37.0 35.0 37.0 7 36.29463612731369 37.0 37.0 37.0 35.0 37.0 8 36.30532982586873 37.0 37.0 37.0 35.0 37.0 9 38.103934334609555 39.0 39.0 39.0 37.0 39.0 10-11 38.066937863487254 39.0 39.0 39.0 35.0 39.0 12-13 38.0392614410155 39.0 39.0 39.0 35.0 39.0 14-15 39.529332189874175 41.0 39.0 41.0 37.0 41.0 16-17 39.53020477340665 41.0 39.0 41.0 37.0 41.0 18-19 39.5478413996454 41.0 39.5 41.0 37.0 41.0 20-21 39.5458877225891 41.0 40.0 41.0 37.0 41.0 22-23 39.47230136144444 41.0 39.0 41.0 36.5 41.0 24-25 39.41796537074629 41.0 39.0 41.0 36.0 41.0 26-27 39.30631001019263 41.0 39.0 41.0 36.0 41.0 28-29 39.2534539318721 41.0 39.0 41.0 36.0 41.0 30-31 39.24676046671977 41.0 39.0 41.0 36.0 41.0 32-33 39.19948785235244 41.0 39.0 41.0 35.0 41.0 34-35 39.120794200906204 41.0 39.0 41.0 35.0 41.0 36-37 39.03004685184709 41.0 39.0 41.0 35.0 41.0 38-39 38.93195588260486 41.0 39.0 41.0 35.0 41.0 40-41 38.82408207424348 41.0 39.0 41.0 35.0 41.0 42-43 38.70508951743454 41.0 39.0 41.0 35.0 41.0 44-45 38.53781712790469 41.0 38.0 41.0 35.0 41.0 46-47 38.330515370318025 40.5 38.0 41.0 34.5 41.0 48-49 38.11567218910331 40.0 38.0 41.0 34.0 41.0 50-51 37.97528366994715 40.0 38.0 41.0 34.0 41.0 52-53 37.83967508843607 40.0 38.0 41.0 33.0 41.0 54-55 37.688470816095794 40.0 37.5 41.0 33.0 41.0 56-57 37.545793772644345 40.0 37.0 41.0 33.0 41.0 58-59 37.37073986518317 40.0 37.0 41.0 33.0 41.0 60-61 37.17829825868729 40.0 36.0 41.0 33.0 41.0 62-63 36.946547834708056 39.0 36.0 41.0 32.0 41.0 64-65 36.676663369050374 39.0 35.0 41.0 32.0 41.0 66-67 36.400772530856266 39.0 35.0 41.0 31.5 41.0 68-69 36.09314936274636 38.0 35.0 40.5 31.0 41.0 70-71 35.763660214901805 37.0 35.0 40.0 31.0 41.0 72-73 35.419267822117156 37.0 35.0 39.5 31.0 41.0 74-75 35.06964340776524 36.0 35.0 39.0 31.0 41.0 76-77 34.718039888309306 36.0 35.0 39.0 30.0 40.5 78-79 34.35413427936378 35.0 34.5 37.5 30.0 39.5 80-81 34.01966230481966 35.0 34.0 37.0 30.0 39.0 82-83 33.71802302549871 35.0 34.0 37.0 30.0 39.0 84-85 33.484961317247816 35.0 34.0 36.0 30.0 37.5 86-87 33.2688004278336 35.0 34.0 36.0 30.0 37.0 88-89 33.058980222867476 35.0 34.0 36.0 29.5 37.0 90-91 32.87056695445863 35.0 34.0 35.0 29.0 36.0 92-93 32.68812820661065 35.0 34.0 35.0 29.0 36.0 94-95 32.5257851110483 35.0 34.0 35.0 29.0 36.0 96-97 32.37902352014115 35.0 34.0 35.0 29.0 36.0 98-99 32.24747539635635 35.0 34.0 35.0 29.0 35.5 100 32.13956732704645 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10146820049236993 1101 2 0.0652324064673806 1101 3 0.10430428582066753 1101 4 0.1669381696999892 1101 5 0.11048831046385743 1101 6 0.12481631443803565 1101 7 0.13801981083763337 1101 8 0.13305523803114738 1101 9 0.24782803378999319 1101 10-11 0.23548628800161708 1101 12-13 0.23999739743570103 1101 14-15 0.30737601422179495 1101 16-17 0.2715390335393906 1101 18-19 0.2744378502658833 1101 20-21 0.237008467219475 1101 22-23 0.22037158797115808 1101 24-25 0.2429625632431467 1101 26-27 0.24584259765711636 1101 28-29 0.3066228466322656 1101 30-31 0.34933958192803516 1101 32-33 0.33118896104170403 1101 34-35 0.31834666369508113 1101 36-37 0.3713824931957461 1101 38-39 0.34553214348211725 1101 40-41 0.3621180229969596 1101 42-43 0.3039014985990889 1101 44-45 0.29998973216407876 1101 46-47 0.30688242743695326 1101 48-49 0.2911260490851504 1101 50-51 0.39064269981430044 1101 52-53 0.41710693372342433 1101 54-55 0.3990491668578997 1101 56-57 0.42309747353296245 1101 58-59 0.3835165920264032 1101 60-61 0.3970678708678719 1101 62-63 0.3924776775491523 1101 64-65 0.40522022778029765 1101 66-67 0.4776567477947893 1101 68-69 0.44498467286999244 1101 70-71 0.4062970900411145 1101 72-73 0.45565224739272026 1101 74-75 0.4086987151209769 1101 76-77 0.5012545848197121 1101 78-79 0.4116574456241153 1101 80-81 0.4119221157419375 1101 82-83 0.3587250841729883 1101 84-85 0.3763716894324176 1101 86-87 0.3465277458773386 1101 88-89 0.37604808626821296 1101 90-91 0.5422044359849281 1101 92-93 0.4162512973142398 1101 94-95 0.40224030375852493 1101 96-97 0.33874052162915547 1101 98-99 0.3643280503375905 1101 100 0.3998561718309048 1102 1 0.09520055331292099 1102 2 0.062332511522321 1102 3 0.11998586524126154 1102 4 0.0648619938260282 1102 5 0.07504346746262769 1102 6 0.09235339126064446 1102 7 0.08673091605977845 1102 8 0.07090530286235008 1102 9 0.14540924630698981 1102 10-11 0.1405399669751759 1102 12-13 0.1575715431939102 1102 14-15 0.246035286030164 1102 16-17 0.27668798756444346 1102 18-19 0.18922220370478016 1102 20-21 0.18919080221539275 1102 22-23 0.2121063195131896 1102 24-25 0.21220386466249153 1102 26-27 0.14745375556447726 1102 28-29 0.20681276577466434 1102 30-31 0.22151185310229238 1102 32-33 0.2206642226537454 1102 34-35 0.24961202364233515 1102 36-37 0.19377118385784797 1102 38-39 0.21117870458907362 1102 40-41 0.34632618321047914 1102 42-43 0.2422355102150746 1102 44-45 0.3166032403996937 1102 46-47 0.3793350028604081 1102 48-49 0.42852276872095985 1102 50-51 0.4117704157053552 1102 52-53 0.38610168728597927 1102 54-55 0.31828384319088343 1102 56-57 0.3260054132257437 1102 58-59 0.4486479737985789 1102 60-61 0.31364620304970003 1102 62-63 0.2838631723957832 1102 64-65 0.418056490348242 1102 66-67 0.37642243110935425 1102 68-69 0.47606579232030555 1102 70-71 0.45437770787773246 1102 72-73 0.4969615537461749 1102 74-75 0.4922183395732276 1102 76-77 0.4133355875423135 1102 78-79 0.41403111942378246 1102 80-81 0.34642919354253365 1102 82-83 0.3853678689807154 1102 84-85 0.39645595422134505 1102 86-87 0.3128659975485917 1102 88-89 0.39945199254532326 1102 90-91 0.33867657749676994 1102 92-93 0.3311763549682638 1102 94-95 0.3786949045655206 1102 96-97 0.3427878184361717 1102 98-99 0.30589100285464355 1102 100 0.2828044614544396 1103 1 0.10818076503625917 1103 2 0.0197962840977155 1103 3 0.04248650779612717 1103 4 0.05837580966075251 1103 5 0.06775424054362134 1103 6 0.03869764621676097 1103 7 0.05044647602372265 1103 8 0.0803022907581834 1103 9 0.062245149256462184 1103 10-11 0.11755234022896133 1103 12-13 0.09548339673243333 1103 14-15 0.12628251580417071 1103 16-17 0.14944975476530686 1103 18-19 0.22294200513421458 1103 20-21 0.14845795179372345 1103 22-23 0.17447417342818738 1103 24-25 0.13831910461601638 1103 26-27 0.04340149684836092 1103 28-29 0.15342942779562918 1103 30-31 0.06376086151643534 1103 32-33 0.010561751743686898 1103 34-35 0.0760558414198087 1103 36-37 0.1629626386624068 1103 38-39 0.144603081612523 1103 40-41 0.07186068026196324 1103 42-43 0.0467683577407243 1103 44-45 0.033613628671766094 1103 46-47 0.05557211707107257 1103 48-49 -0.017529772357299578 1103 50-51 -0.04473012790209907 1103 52-53 -0.08363427997931439 1103 54-55 -0.06945156417683762 1103 56-57 -0.18668821299193894 1103 58-59 -0.14947528330903737 1103 60-61 -0.14611870030159935 1103 62-63 -0.16810705770522816 1103 64-65 -0.11353081325018621 1103 66-67 -0.20697440353865915 1103 68-69 -0.22469172858836828 1103 70-71 -0.13653460540426465 1103 72-73 -0.14743244159364366 1103 74-75 -0.10846533277567971 1103 76-77 -0.1558660636728746 1103 78-79 -0.1311548172522805 1103 80-81 -0.0711391616255952 1103 82-83 -0.062372208222399195 1103 84-85 0.0017580274110571281 1103 86-87 -0.027965619288394805 1103 88-89 -0.10983322700330689 1103 90-91 -0.15910494902053074 1103 92-93 -0.06202725468175885 1103 94-95 -0.09632585025846652 1103 96-97 -0.048872603683157934 1103 98-99 -0.022885319269875026 1103 100 -0.045664158337700655 1104 1 0.0754326037649875 1104 2 0.07482523097849736 1104 3 0.08891148575909114 1104 4 0.08311833568524207 1104 5 0.12367159603002165 1104 6 0.12692848352022423 1104 7 0.18346834680789925 1104 8 0.13942389190255255 1104 9 0.1864275275938425 1104 10-11 0.1899582910080042 1104 12-13 0.16653941669650862 1104 14-15 0.2748314051202314 1104 16-17 0.2664166273418829 1104 18-19 0.2547487217110813 1104 20-21 0.23526915370838708 1104 22-23 0.2880997724112859 1104 24-25 0.2782923830862174 1104 26-27 0.266235329803294 1104 28-29 0.27500935772738444 1104 30-31 0.2767276007451329 1104 32-33 0.2184982885433655 1104 34-35 0.1705120331775234 1104 36-37 0.22394245117874334 1104 38-39 0.2789861398509075 1104 40-41 0.3122325736459999 1104 42-43 0.23659144666644494 1104 44-45 0.3027274021223789 1104 46-47 0.19748067159736138 1104 48-49 0.16246778581787424 1104 50-51 0.26369182981796513 1104 52-53 0.2766916083859954 1104 54-55 0.21764598932250578 1104 56-57 0.20848909639418878 1104 58-59 0.23155632706576768 1104 60-61 0.28529156609975814 1104 62-63 0.2501838121212643 1104 64-65 0.2519915294469328 1104 66-67 0.21519178082431267 1104 68-69 0.28968364915846223 1104 70-71 0.23162568740689693 1104 72-73 0.262623763672039 1104 74-75 0.20763040580705905 1104 76-77 0.22383509853182915 1104 78-79 0.15753316948912754 1104 80-81 0.1721298010491239 1104 82-83 0.2287716741405248 1104 84-85 0.28552578043000665 1104 86-87 0.3087520659309533 1104 88-89 0.22888445994782813 1104 90-91 0.2500907727638264 1104 92-93 0.22434749269327625 1104 94-95 0.31631418666331257 1104 96-97 0.34502880893847276 1104 98-99 0.2727439008874768 1104 100 0.2917380171377246 1105 1 0.04140924909042809 1105 2 0.08649691403894622 1105 3 0.07409095543490452 1105 4 0.007808439771103792 1105 5 0.013483041682306407 1105 6 0.039004443099464936 1105 7 0.05172453800317811 1105 8 0.07217759903479504 1105 9 0.04081502763693834 1105 10-11 0.011113199316604039 1105 12-13 0.030553014398968514 1105 14-15 0.06768550908197568 1105 16-17 0.11371249803959671 1105 18-19 0.11606711963695204 1105 20-21 0.09874642823786672 1105 22-23 0.17540309012656508 1105 24-25 0.12928473265473883 1105 26-27 0.08533782627802822 1105 28-29 0.11085371506010233 1105 30-31 0.09570191198219646 1105 32-33 0.12577574855874474 1105 34-35 0.10308046127070725 1105 36-37 0.09382271278971643 1105 38-39 0.05855635040154539 1105 40-41 0.09620114447369588 1105 42-43 0.12503926065648585 1105 44-45 0.1660908338183944 1105 46-47 0.0883444222050187 1105 48-49 0.09277631989315438 1105 50-51 0.0274508498456143 1105 52-53 0.05938577385458643 1105 54-55 0.009427929615355879 1105 56-57 0.10723475661022519 1105 58-59 0.0298354454216323 1105 60-61 0.03925975768117951 1105 62-63 0.15505864361525568 1105 64-65 0.12345131727588665 1105 66-67 0.056431295526998326 1105 68-69 0.12478902790481783 1105 70-71 0.04324082829688081 1105 72-73 0.028278532349048646 1105 74-75 0.05977752577643969 1105 76-77 0.06679970106450384 1105 78-79 0.13644245723016724 1105 80-81 0.07121064600980276 1105 82-83 0.12981384287753173 1105 84-85 0.06915751162024719 1105 86-87 0.08347295791816833 1105 88-89 0.08608603623320477 1105 90-91 0.05215690641711035 1105 92-93 0.134893572838358 1105 94-95 0.13083173369846435 1105 96-97 0.05441901542082661 1105 98-99 -0.00409573655309714 1105 100 0.09815100252984621 1106 1 0.050542054255714675 1106 2 0.044932175020946374 1106 3 0.0453979242883662 1106 4 0.033594021112826056 1106 5 0.04543600068614495 1106 6 0.0801514791543454 1106 7 0.11486291034518104 1106 8 0.08496629338361572 1106 9 0.11520634415893483 1106 10-11 0.16370292474608306 1106 12-13 0.13576178352584378 1106 14-15 0.15970237532149412 1106 16-17 0.15978105007952337 1106 18-19 0.17053354363181228 1106 20-21 0.1599155353483681 1106 22-23 0.17927903102884812 1106 24-25 0.15038795601711286 1106 26-27 0.031948601565034096 1106 28-29 0.10843512266638555 1106 30-31 0.10172434539843067 1106 32-33 0.08042071751102498 1106 34-35 0.04349717597760616 1106 36-37 0.04330140763266854 1106 38-39 0.06752279910280379 1106 40-41 0.13584668990877447 1106 42-43 0.21842782653200032 1106 44-45 0.15059827044647278 1106 46-47 0.15545229037163466 1106 48-49 0.23029349084500694 1106 50-51 0.18651595945969746 1106 52-53 0.11070394294522856 1106 54-55 0.15119130813395998 1106 56-57 0.12241503101603257 1106 58-59 0.09300445370651289 1106 60-61 0.1339554571195265 1106 62-63 0.15140242501297507 1106 64-65 0.15638034400821965 1106 66-67 0.14707481503682374 1106 68-69 0.10167931499324823 1106 70-71 0.11791808028574735 1106 72-73 0.20215892040432948 1106 74-75 0.12714629358564622 1106 76-77 0.11843641807734429 1106 78-79 0.1870408463725255 1106 80-81 0.1474743786535484 1106 82-83 0.10940536363914788 1106 84-85 0.17563553115478214 1106 86-87 0.1274694660795106 1106 88-89 0.1953028431773518 1106 90-91 0.15863064350801892 1106 92-93 0.1815036892878794 1106 94-95 0.22924887664972715 1106 96-97 0.21670155787466427 1106 98-99 0.17232809359860113 1106 100 0.2119296001942672 1107 1 -0.09209489764674572 1107 2 -0.055742421187751745 1107 3 -0.014591771405058296 1107 4 0.022020624474968997 1107 5 0.0013334417833448242 1107 6 -0.021754437330073984 1107 7 -0.02137371100830876 1107 8 0.015668500824645548 1107 9 0.003513110843705647 1107 10-11 -0.029821395255233085 1107 12-13 -0.0745964527266807 1107 14-15 -0.09645831355879153 1107 16-17 -0.006463410405110892 1107 18-19 0.01248088013332449 1107 20-21 0.03084693439563324 1107 22-23 0.045551375245914016 1107 24-25 0.024763186137121806 1107 26-27 0.02432823097755943 1107 28-29 0.007350572218690843 1107 30-31 -0.002077664891928066 1107 32-33 -0.028253468046337105 1107 34-35 -0.07531382364786765 1107 36-37 -0.0485222581782665 1107 38-39 0.0686335856078415 1107 40-41 0.06413023704907062 1107 42-43 0.1252005316532987 1107 44-45 0.14598344935546947 1107 46-47 0.11153953096579272 1107 48-49 0.1315244942601268 1107 50-51 0.1804312220642572 1107 52-53 0.029508605358778084 1107 54-55 0.1605962375976162 1107 56-57 0.19202074870212016 1107 58-59 0.1265407890616288 1107 60-61 0.15246467871344294 1107 62-63 0.2388819320539426 1107 64-65 0.11221701124312489 1107 66-67 0.01287180498790974 1107 68-69 0.06285856873584805 1107 70-71 0.0408606885934546 1107 72-73 0.10595674813482958 1107 74-75 0.029922119150086246 1107 76-77 0.03596837822134802 1107 78-79 -0.018887678749131 1107 80-81 -0.001641421853555869 1107 82-83 -0.017629752753258288 1107 84-85 -0.06551445734318406 1107 86-87 -0.13571006523296347 1107 88-89 -0.09276893968948485 1107 90-91 -0.12777502297339538 1107 92-93 -0.02918813780001983 1107 94-95 -0.0583087048420694 1107 96-97 -0.033218688313340294 1107 98-99 0.07840079029139702 1107 100 0.10018845755693206 1108 1 -0.01364914583191279 1108 2 -0.05417510984398888 1108 3 0.04265250807888776 1108 4 0.02182119715624964 1108 5 0.03939400563336193 1108 6 0.07979860139350592 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-0.1285289268216232 2214 54-55 -0.11528815809620596 2214 56-57 -0.2021868652830463 2214 58-59 -0.05921501608590063 2214 60-61 -0.04207702276630698 2214 62-63 -0.061612694896197695 2214 64-65 -0.1273738124096795 2214 66-67 -0.09756000479072213 2214 68-69 -0.18762611110582839 2214 70-71 -0.13611797496633216 2214 72-73 -0.12689437244023338 2214 74-75 -0.1086138705839872 2214 76-77 -0.13524466843188065 2214 78-79 -0.06774366459888626 2214 80-81 -0.0775299102561462 2214 82-83 -0.10583944295560599 2214 84-85 -0.11486226647643605 2214 86-87 -0.12455866916811686 2214 88-89 -0.04094428980943832 2214 90-91 -0.028891634236316577 2214 92-93 -0.08521726714444355 2214 94-95 -0.10323466978140772 2214 96-97 -0.11179466843115193 2214 98-99 -0.12939779644577243 2214 100 -0.17800047691869025 2215 1 -0.1614383393058958 2215 2 -0.07873380979732048 2215 3 -0.16897082773659378 2215 4 -0.08327985546798544 2215 5 -0.12142349399041308 2215 6 -0.17955662700014585 2215 7 -0.18598663782778146 2215 8 -0.14095396136321625 2215 9 -0.22172307116586865 2215 10-11 -0.2032768646686165 2215 12-13 -0.26946403375660566 2215 14-15 -0.3317511914442193 2215 16-17 -0.3107441193222016 2215 18-19 -0.3574457293174831 2215 20-21 -0.3323865637680825 2215 22-23 -0.37378335221843173 2215 24-25 -0.3540685630487985 2215 26-27 -0.39552693048523224 2215 28-29 -0.412199434750562 2215 30-31 -0.4254417351209767 2215 32-33 -0.3712046515264973 2215 34-35 -0.3088049445245744 2215 36-37 -0.33397042532766363 2215 38-39 -0.3604654023892806 2215 40-41 -0.3546608255718269 2215 42-43 -0.294378697826879 2215 44-45 -0.38520417030703413 2215 46-47 -0.3916893462569888 2215 48-49 -0.3001226861775237 2215 50-51 -0.2778316741556779 2215 52-53 -0.20791094601471372 2215 54-55 -0.313872800941823 2215 56-57 -0.2721955508576315 2215 58-59 -0.33073900092312414 2215 60-61 -0.2400135100458911 2215 62-63 -0.306228164281535 2215 64-65 -0.2869901862402955 2215 66-67 -0.3044337344438759 2215 68-69 -0.26300990221413656 2215 70-71 -0.24776621499560036 2215 72-73 -0.26525628249814304 2215 74-75 -0.31422119561189277 2215 76-77 -0.3635600040898268 2215 78-79 -0.43165038964788494 2215 80-81 -0.4176780000278697 2215 82-83 -0.3729145177128146 2215 84-85 -0.39124732743314183 2215 86-87 -0.32869368780259123 2215 88-89 -0.34146580491295 2215 90-91 -0.30326009037543145 2215 92-93 -0.3430734346092805 2215 94-95 -0.3081295855664976 2215 96-97 -0.2753993015109373 2215 98-99 -0.2974054829617785 2215 100 -0.3335302625307257 2216 1 -0.1355798490524549 2216 2 -0.13314684520407383 2216 3 -0.14183805816296768 2216 4 -0.15693707450078165 2216 5 -0.179425123586185 2216 6 -0.16364079502692874 2216 7 -0.1614210338535429 2216 8 -0.17118483209242896 2216 9 -0.19617852024354931 2216 10-11 -0.19844143429985195 2216 12-13 -0.25629905800087727 2216 14-15 -0.27098192538255006 2216 16-17 -0.26391605357081716 2216 18-19 -0.29250438371998655 2216 20-21 -0.35842118290742064 2216 22-23 -0.2942606929356373 2216 24-25 -0.31292861917554404 2216 26-27 -0.2577311839171017 2216 28-29 -0.34437171649899767 2216 30-31 -0.34465694626748444 2216 32-33 -0.3335905564576862 2216 34-35 -0.3718732745457558 2216 36-37 -0.37183316544452794 2216 38-39 -0.33256197335613535 2216 40-41 -0.3135372371305749 2216 42-43 -0.371071145185752 2216 44-45 -0.3119143749920781 2216 46-47 -0.21846289106959915 2216 48-49 -0.3101311910021636 2216 50-51 -0.3106512061949047 2216 52-53 -0.2772834666799042 2216 54-55 -0.32764963978092254 2216 56-57 -0.34394874271576725 2216 58-59 -0.2496127871164191 2216 60-61 -0.41838423713618056 2216 62-63 -0.4286427528170975 2216 64-65 -0.48519478462310417 2216 66-67 -0.45904698582729253 2216 68-69 -0.41877364141424067 2216 70-71 -0.47168359160129825 2216 72-73 -0.4777587145721185 2216 74-75 -0.4924089967536247 2216 76-77 -0.5029556942423241 2216 78-79 -0.516078319118165 2216 80-81 -0.47183402150318443 2216 82-83 -0.34647402897002877 2216 84-85 -0.5204183236871103 2216 86-87 -0.5470852224009093 2216 88-89 -0.6311195285562761 2216 90-91 -0.6550095268564604 2216 92-93 -0.5622478633798877 2216 94-95 -0.5294556670004056 2216 96-97 -0.623344337063827 2216 98-99 -0.7402084981387702 2216 100 -0.7553856227368101 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 1.0 4 5.0 5 17.0 6 42.0 7 104.0 8 229.0 9 443.0 10 786.0 11 1255.0 12 2058.0 13 3125.0 14 3953.0 15 5190.0 16 6526.0 17 8909.0 18 10814.0 19 5735.0 20 3835.0 21 3617.0 22 3795.0 23 3991.0 24 4520.0 25 5565.0 26 6911.0 27 9054.0 28 11640.0 29 14998.0 30 19447.0 31 24845.0 32 31857.0 33 41134.0 34 56700.0 35 86307.0 36 155029.0 37 356660.0 38 657613.0 39 319930.0 40 1368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14984599746034 19.695508245870638 13.558275407205636 23.59637034946339 2 15.769900933354727 19.99717734383762 40.3559337223161 23.87698800049155 3 18.486905501188463 25.016542110109423 29.89228891410951 26.604263474592603 4 12.227665448409477 16.82908013998091 38.910281701677064 32.03297270993255 5 12.30960384108676 37.61269327354788 35.90146321251512 14.176239672850244 6 30.88915238903328 38.37859974357807 17.362655010800236 13.369592856588412 7 27.107048333633116 32.41540757680876 22.288513588748703 18.189030500809412 8 25.053559670451442 36.197664894727545 20.13950674782255 18.609268686998465 9 24.737047220152288 16.445737081481102 21.42861731377033 37.38859838459628 10-11 23.82659836182952 26.640198051550897 28.7652265245395 20.76797706208008 12-13 24.918228751787993 23.889383174448184 29.13387786828378 22.05851020548004 14-15 21.86924438099016 25.90340048763083 26.68875432512059 25.538600806258422 16-17 20.832574951338643 29.481582559426812 27.147229246921327 22.538613242313218 18-19 20.58105715764753 27.831289892072252 30.17108054618581 21.416572404094413 20-21 21.892906698871958 27.17875542821903 29.844283989002236 21.084053883906776 22-23 21.389523103401203 27.082036279151932 29.986777408104327 21.541663209342538 24-25 21.237262412372623 27.18395927183959 29.971172299711725 21.607606016076062 26-27 21.036812635454723 27.000875529004475 30.111399087854558 21.850912747686245 28-29 21.096027029826654 27.110848680989747 30.127212882368703 21.665911406814896 30-31 21.422612083578578 27.74863364283307 29.56101250820055 21.267741765387797 32-33 20.91850391003075 27.318877354369558 30.222332142765374 21.540286592834324 34-35 21.056056265042695 26.990293445024026 30.273884163733072 21.679766126200203 36-37 21.61068213548492 27.567322763830717 29.409303774700003 21.41269132598436 38-39 21.485147878819024 27.657900146465554 29.566850605134004 21.290101369581414 40-41 21.17177859920825 27.45095729959342 29.736565284540006 21.640698816658325 42-43 21.197302378566352 27.324739188529435 29.635506333992858 21.84245209891136 44-45 21.456507867170302 27.547756550265095 29.349989507584517 21.645746074980085 46-47 21.524735334172725 27.930649416279092 28.973359971220802 21.57125527832738 48-49 21.522995520380984 27.791303714743343 28.840304365701364 21.845396399174312 50-51 21.581480030149635 27.698585022826354 28.69651544740516 22.023419499618846 52-53 21.631533134619833 27.729259278293117 28.6317408416202 22.007466745466846 54-55 21.695585048522066 28.26338211235878 28.416753389692595 21.624279449426556 56-57 21.44074247758049 27.911324100007707 28.567903058646177 22.08003036376562 58-59 21.220776481571892 27.465102011974203 29.01950518625108 22.294616320202827 60-61 21.633219415679523 27.91544612037584 28.53736263500955 21.913971828935082 62-63 21.474628696970477 27.91673090594513 28.539557477023752 22.06908292006064 64-65 21.43886883195861 27.597515224708996 28.79389148701082 22.169724456321575 66-67 21.30958728404896 27.725485220683332 28.540306935272504 22.424620559995205 68-69 21.859258014126233 27.828378159906723 28.487072366695326 21.825291459271718 70-71 21.925856095451003 27.606160760935666 28.472507730126505 21.995475413486822 72-73 21.6379650462818 27.798387912717953 28.587033387883277 21.976613653116974 74-75 21.646753812671154 27.84081708145199 28.467311147745374 22.04511795813148 76-77 21.66293008196932 27.86330523935555 28.265817851667226 22.207946827007905 78-79 21.884903555429933 28.04065918064942 28.278933371020376 21.795503892900275 80-81 21.771681827136387 27.70926480287107 28.434097994877988 22.08495537511456 82-83 21.566743307735752 27.83039750751466 28.582506029127174 22.020353155622413 84-85 21.573771316733904 27.723906005087752 28.46265770742863 22.239664970749715 86-87 21.74447907730563 27.895471511757847 28.294024515426198 22.06602489551032 88-89 21.435815680384756 27.764993641539128 28.414035777082614 22.385154900993502 90-91 21.337709530702618 27.59456183778876 28.536245492466744 22.531483139041878 92-93 21.574687695478904 27.92468556754426 28.20024641215498 22.300380324821862 94-95 21.28186075992058 27.651014903017167 28.474323759335462 22.59280057772679 96-97 21.26791374901734 27.62592454497786 28.507877485896117 22.598284220108688 98-99 21.289047809853727 27.67095758625 28.366455399569972 22.673539204326303 100 21.410094988479756 27.63691531550051 28.254361847007736 22.698627849012 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 37.0 1 30.0 2 74.0 3 130.5 4 94.0 5 68.0 6 179.5 7 441.5 8 757.5 9 886.0 10 725.0 11 569.5 12 593.5 13 700.0 14 850.5 15 980.0 16 1125.5 17 1372.5 18 1662.5 19 1944.0 20 2381.5 21 2999.0 22 3829.0 23 5109.5 24 6828.5 25 8908.5 26 11695.5 27 15309.0 28 19811.5 29 24959.0 30 30259.5 31 35705.5 32 41240.5 33 46612.0 34 51983.5 35 56429.0 36 59681.5 37 63082.0 38 65544.0 39 66475.5 40 67231.0 41 67667.5 42 67210.5 43 66984.5 44 66174.5 45 64631.0 46 63903.5 47 62793.5 48 61319.5 49 59834.5 50 58396.0 51 56290.0 52 52526.0 53 49351.0 54 47229.5 55 44819.5 56 41415.5 57 38000.0 58 34597.0 59 30764.5 60 26983.5 61 22872.0 62 18976.5 63 15507.0 64 12370.0 65 9761.5 66 7553.0 67 5678.5 68 4314.5 69 3263.0 70 2373.5 71 1662.0 72 1156.5 73 821.0 74 568.0 75 358.5 76 233.5 77 155.0 78 94.5 79 56.0 80 38.0 81 21.5 82 5.5 83 3.0 84 1.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04491396219304331 2 0.24212854707882586 3 0.0029978329950064667 4 0.0025160384065232846 5 1.6059819616106073E-4 6 5.3532732053686904E-5 7 0.0 8 3.2119639232212145E-4 9 0.0 10-11 9.36822810939521E-4 12-13 0.0 14-15 2.4089729424159106E-4 16-17 2.1413092821474762E-4 18-19 3.7472912437580834E-4 20-21 0.0 22-23 5.3532732053686904E-5 24-25 0.001124187373127425 26-27 1.3383183013421728E-4 28-29 2.9443002629527795E-4 30-31 8.029909808053036E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.3532732053686904E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.0706546410737381E-4 70-71 0.0 72-73 1.6059819616106073E-4 74-75 8.029909808053036E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.3532732053686904E-5 84-85 0.0 86-87 4.5502822245633873E-4 88-89 0.0017398137917448246 90-91 0.001713047425717981 92-93 4.014954904026518E-4 94-95 2.6766366026843456E-4 96-97 1.3383183013421728E-4 98-99 8.029909808053036E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1868016.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.46402306198632 #Duplication Level Percentage of deduplicated Percentage of total 1 81.69238679127928 59.197590004319686 2 12.191731284264115 17.669237938969097 3 3.1667469319078587 6.884256681157365 4 1.204075526646716 3.490086269252038 5 0.564507609669957 2.0453246222895265 6 0.30487699057468653 1.3255567965643842 7 0.1973454104021002 1.001030965938846 8 0.1324157671471318 0.7676303363456297 9 0.09990312185145742 0.6515443913227595 >10 0.42563463866771445 5.185461190516102 >50 0.016010287835736714 0.7734150380556291 >100 0.0040698472695159926 0.5273975012087475 >500 7.394812095606755E-5 0.053103511372698746 >1k 2.2184436286820267E-4 0.42836475268754165 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3534 0.18918467507772954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2994 0.1602769997687386 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.676636602684345E-4 0.0 10-11 0.0 0.0 0.0 2.676636602684345E-4 0.0 12-13 0.0 0.0 0.0 2.676636602684345E-4 0.0 14-15 0.0 0.0 0.0 3.479627583489649E-4 0.0 16-17 0.0 0.0 0.0 4.5502822245633873E-4 0.0 18-19 0.0 0.0 0.0 4.817945884831822E-4 0.0 20-21 0.0 0.0 0.0 4.817945884831822E-4 0.0 22-23 0.0 0.0 0.0 5.085609545100256E-4 0.0 24-25 0.0 0.0 0.0 6.423927846442429E-4 0.0 26-27 0.0 0.0 0.0 8.565237128589905E-4 0.0 28-29 0.0 0.0 0.0 0.0011777201051811118 0.0 30-31 0.0 0.0 0.0 0.0028907675308990927 0.0 32-33 0.0 0.0 0.0 0.00728045155930142 0.0 34-35 0.0 0.0 0.0 0.013811444869851221 0.0 36-37 0.0 0.0 0.0 0.025401281359474434 0.0 38-39 0.0 0.0 0.0 0.0463861123245197 2.6766366026843452E-5 40-41 0.0 0.0 0.0 0.07572204948994013 5.3532732053686904E-5 42-43 0.0 0.0 0.0 0.1082967169446086 5.3532732053686904E-5 44-45 0.0 0.0 0.0 0.14378891829620305 5.3532732053686904E-5 46-47 0.0 0.0 0.0 0.1839384673364682 5.3532732053686904E-5 48-49 0.0 0.0 0.0 0.2316093652302764 5.3532732053686904E-5 50-51 0.0 0.0 0.0 0.2844461717672654 5.3532732053686904E-5 52-53 0.0 0.0 0.0 0.3393439884883213 5.3532732053686904E-5 54-55 0.0 0.0 0.0 0.40053190122568544 5.3532732053686904E-5 56-57 0.0 0.0 0.0 0.46841140546976046 5.3532732053686904E-5 58-59 0.0 0.0 0.0 0.5505841491721699 5.3532732053686904E-5 60-61 0.0 0.0 0.0 0.6430351774288872 5.3532732053686904E-5 62-63 0.0 0.0 0.0 0.7330504663771618 5.3532732053686904E-5 64-65 0.0 0.0 0.0 0.8306406369110328 5.3532732053686904E-5 66-67 0.0 0.0 0.0 0.9423366823410506 5.3532732053686904E-5 68-69 0.0 0.0 0.0 1.0723944548654831 5.3532732053686904E-5 70-71 0.0 0.0 0.0 1.2158354104033369 5.3532732053686904E-5 72-73 0.0 0.0 0.0 1.3718833243398343 1.6059819616106073E-4 74-75 0.0 0.0 0.0 1.5299119493623181 1.6059819616106073E-4 76-77 0.0 0.0 0.0 1.6917413983606135 1.6059819616106073E-4 78-79 0.0 0.0 0.0 1.8814614007588801 1.6059819616106073E-4 80-81 0.0 0.0 0.0 2.094842870724876 1.6059819616106073E-4 82-83 0.0 0.0 0.0 2.327603189694307 2.1413092821474762E-4 84-85 0.0 0.0 0.0 2.550647317795993 2.1413092821474762E-4 86-87 0.0 0.0 0.0 2.7849868523610075 2.1413092821474762E-4 88 0.0 0.0 0.0 2.9801136606966963 2.1413092821474762E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2755 0.0 53.879646 1 GTATCAA 5095 0.0 41.99015 1 TCAACGC 5790 0.0 36.447086 4 ATCAACG 5915 0.0 35.67877 3 CAACGCA 5980 0.0 35.287178 5 AACGCAG 6135 0.0 34.319046 6 TATCAAC 6315 0.0 33.489655 2 ACGCAGA 7075 0.0 29.56006 7 CGCAGAG 7230 0.0 28.92634 8 GCAGAGT 8185 0.0 25.034538 9 GTACATG 4760 0.0 24.353643 1 GTGGTAT 1395 0.0 22.970503 1 TACATGG 4730 0.0 22.70447 2 GAGTACT 5825 0.0 22.106827 12-13 CAGAGTA 8095 0.0 21.36499 10-11 ACATGGG 4965 0.0 20.921427 3 GAGTACA 3660 0.0 20.729107 1 GGGTACG 140 5.120943E-4 20.195704 1 AGAGTAC 7500 0.0 20.052128 10-11 GTACTTT 6480 0.0 19.94479 14-15 >>END_MODULE