FastQCFastQC Report
Wed 25 May 2016
SRR1033038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4753815
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72250.034.9427721
GGTATCA47100.033.9374281
TCAACGC77650.030.4526144
CAACGCA80350.029.4878235
ATCAACG81150.029.1971243
GTACATG168200.028.845271
AACGCAG83950.028.3841256
TACATGG170150.027.4356542
TATCAAC90600.026.7225022
ACATGGG180800.025.3775373
CATGGGG136800.022.8181294
ACGCAGA103750.022.4689037
CGCAGAG107000.021.7228368
GAGTACA118100.020.5807671
AGTACAT120800.019.263552
CGTACGA1550.001032373218.1977462
GCAGAGT124900.017.89699
AGAGTAC101100.017.04310-11
GTATTAG18950.016.6221241
GTCTATA11200.016.370711
Produced by FastQC (version 0.11.5)