FastQCFastQC Report
Wed 25 May 2016
SRR1033038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4753815
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63120.1327775691733902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52580.1106059028380364No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68950.047.0893481
GTATCAA120300.042.853811
TCAACGC144000.035.150314
ATCAACG144750.034.970023
CAACGCA146150.034.6332175
AACGCAG151550.033.3991746
TATCAAC153300.033.3343352
GTACATG168700.029.5813941
TACATGG169900.028.4418752
ACGCAGA177800.028.3095957
CGCAGAG181450.027.4552158
ACATGGG178500.026.2252673
GCAGAGT207000.023.6577439
CATGGGG134950.023.4030424
GAGTACT110100.020.66017512-13
AGAGTAC180350.020.26096710-11
GAGTACA116250.020.1060371
CAGAGTA196800.019.40323810-11
AGTACTT116950.019.04820612-13
AGTACAT119700.018.8077982
Produced by FastQC (version 0.11.5)