##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033038_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4753815
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.735903479626366	34.0	31.0	34.0	31.0	34.0
2	32.736988923632914	34.0	31.0	34.0	31.0	34.0
3	32.8452951997501	34.0	31.0	34.0	31.0	34.0
4	36.322776128225435	37.0	37.0	37.0	35.0	37.0
5	36.306935377165495	37.0	37.0	37.0	35.0	37.0
6	36.36695369929204	37.0	37.0	37.0	35.0	37.0
7	36.316743710051824	37.0	37.0	37.0	35.0	37.0
8	36.328118784597216	37.0	37.0	37.0	35.0	37.0
9	38.140318249658435	39.0	39.0	39.0	37.0	39.0
10-11	38.10221516823856	39.0	39.0	39.0	36.0	39.0
12-13	38.07554069731363	39.0	39.0	39.0	35.0	39.0
14-15	39.5801548861283	41.0	40.0	41.0	37.0	41.0
16-17	39.57183966982308	41.0	40.0	41.0	37.0	41.0
18-19	39.58262605928081	41.0	40.0	41.0	37.0	41.0
20-21	39.57176404635014	41.0	40.0	41.0	37.0	41.0
22-23	39.5035160181875	41.0	39.5	41.0	36.5	41.0
24-25	39.44551586462662	41.0	39.0	41.0	36.0	41.0
26-27	39.33952183667223	41.0	39.0	41.0	36.0	41.0
28-29	39.29131308222975	41.0	39.0	41.0	36.0	41.0
30-31	39.29627036390772	41.0	39.0	41.0	36.0	41.0
32-33	39.27190519614247	41.0	39.0	41.0	35.5	41.0
34-35	39.194342333473216	41.0	39.0	41.0	35.0	41.0
36-37	39.11162392730891	41.0	39.0	41.0	35.0	41.0
38-39	39.02840581722259	41.0	39.0	41.0	35.0	41.0
40-41	38.92948011228876	41.0	39.0	41.0	35.0	41.0
42-43	38.81962718364093	41.0	39.0	41.0	35.0	41.0
44-45	38.662115374704314	41.0	38.5	41.0	35.0	41.0
46-47	38.4844770989195	41.0	38.0	41.0	35.0	41.0
48-49	38.298544852923385	40.0	38.0	41.0	34.0	41.0
50-51	38.15687074486492	40.0	38.0	41.0	34.0	41.0
52-53	38.013992340888315	40.0	37.5	41.0	34.0	41.0
54-55	37.84978664504193	40.0	37.0	41.0	34.0	41.0
56-57	37.67645669846218	40.0	37.0	41.0	33.0	41.0
58-59	37.48352901827269	40.0	36.0	41.0	33.0	41.0
60-61	37.2742459477283	39.5	36.0	41.0	33.0	41.0
62-63	37.02711643175007	39.0	35.0	41.0	33.0	41.0
64-65	36.74954368228465	39.0	35.0	41.0	32.5	41.0
66-67	36.45761635654732	38.5	35.0	41.0	32.0	41.0
68-69	36.13967781665883	37.0	35.0	40.0	32.0	41.0
70-71	35.80659407233979	37.0	35.0	39.5	31.5	41.0
72-73	35.459089804714736	36.0	35.0	39.0	31.0	41.0
74-75	35.11303006111933	36.0	35.0	39.0	31.0	41.0
76-77	34.76002305516727	35.5	35.0	38.0	31.0	40.0
78-79	34.41339734507969	35.0	34.5	37.0	31.0	39.0
80-81	34.10124857614358	35.0	34.0	37.0	30.5	39.0
82-83	33.821698782977464	35.0	34.0	36.0	30.0	38.5
84-85	33.61866890066189	35.0	34.0	36.0	30.0	37.0
86-87	33.43160945472215	35.0	34.0	36.0	30.0	37.0
88-89	33.24291174561905	35.0	34.0	35.0	30.0	36.5
90-91	33.08073389477714	35.0	34.0	35.0	30.0	36.0
92-93	32.92213085700642	35.0	34.0	35.0	30.0	36.0
94-95	32.7820902790706	35.0	34.0	35.0	30.0	36.0
96-97	32.651283548055616	35.0	34.0	35.0	29.5	35.5
98-99	32.53694338126326	35.0	34.0	35.0	29.0	35.0
100	32.43964479055243	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09173921762679527
1101	2	0.052118886844773726
1101	3	0.09770673416416997
1101	4	0.09800114325888387
1101	5	0.08413711487357034
1101	6	0.08528475432510163
1101	7	0.1227935654685055
1101	8	0.13855502361171546
1101	9	0.1766512892137868
1101	10-11	0.2088612142440951
1101	12-13	0.19470870045234534
1101	14-15	0.27517053924328394
1101	16-17	0.2886818019725581
1101	18-19	0.28334124122113025
1101	20-21	0.2273658327484256
1101	22-23	0.26946603594885943
1101	24-25	0.27798583754825756
1101	26-27	0.28924791969569696
1101	28-29	0.3255501316157776
1101	30-31	0.31349263750469447
1101	32-33	0.33058494243056913
1101	34-35	0.31721054380766844
1101	36-37	0.33376140305676216
1101	38-39	0.2633954106088723
1101	40-41	0.28000783231159687
1101	42-43	0.24964865986387252
1101	44-45	0.26967166830935696
1101	46-47	0.3009015374208417
1101	48-49	0.2783308120854855
1101	50-51	0.31639585407221915
1101	52-53	0.3201810145232997
1101	54-55	0.2940237150309244
1101	56-57	0.3081971019590952
1101	58-59	0.3008999568461448
1101	60-61	0.29960179185052027
1101	62-63	0.29481422895933207
1101	64-65	0.2986875176959032
1101	66-67	0.2990057421050025
1101	68-69	0.31344567553330904
1101	70-71	0.30118904185516726
1101	72-73	0.2910051254655883
1101	74-75	0.3231894268454951
1101	76-77	0.2923882877033961
1101	78-79	0.25195507297264186
1101	80-81	0.2578996327878684
1101	82-83	0.3286230171150848
1101	84-85	0.30367196733281077
1101	86-87	0.32189875780315447
1101	88-89	0.3228476570861645
1101	90-91	0.3223281214047802
1101	92-93	0.32549804645950076
1101	94-95	0.3622534905759025
1101	96-97	0.3281188580700274
1101	98-99	0.26483015798942944
1101	100	0.2456099535108578
1102	1	0.14213730182114404
1102	2	0.12152729410148311
1102	3	0.12460920029524658
1102	4	0.06527613200458404
1102	5	0.10408286601359151
1102	6	0.11140702413640469
1102	7	0.16442120580961728
1102	8	0.17549514134359612
1102	9	0.1575913622068228
1102	10-11	0.198488171766094
1102	12-13	0.21001910340514485
1102	14-15	0.29902352890035644
1102	16-17	0.28223469353269337
1102	18-19	0.24009091491469547
1102	20-21	0.28217234839959104
1102	22-23	0.30819832814940185
1102	24-25	0.35896332415349974
1102	26-27	0.34131335346777547
1102	28-29	0.3348021324691288
1102	30-31	0.32082123161329434
1102	32-33	0.322767255434421
1102	34-35	0.2848859753174722
1102	36-37	0.3007335017908801
1102	38-39	0.3164267921757826
1102	40-41	0.34380663028613867
1102	42-43	0.27381707844673286
1102	44-45	0.3332611667279579
1102	46-47	0.31676659924357864
1102	48-49	0.3081290652191342
1102	50-51	0.34730169457557736
1102	52-53	0.3472975073809721
1102	54-55	0.3043560311155815
1102	56-57	0.26563442224667
1102	58-59	0.3079647698165857
1102	60-61	0.25361865934837624
1102	62-63	0.2573357027903356
1102	64-65	0.28879338650711617
1102	66-67	0.2695761330490356
1102	68-69	0.29614815561266994
1102	70-71	0.2758219406908822
1102	72-73	0.27561777119630193
1102	74-75	0.3130505736681357
1102	76-77	0.26596894593670584
1102	78-79	0.295192007718704
1102	80-81	0.3081922897915632
1102	82-83	0.3246052480692896
1102	84-85	0.3209639701311957
1102	86-87	0.32979828629233054
1102	88-89	0.3686162863829736
1102	90-91	0.337342342665103
1102	92-93	0.3533793002335557
1102	94-95	0.3822548894171689
1102	96-97	0.40607347577721953
1102	98-99	0.3802655212534347
1102	100	0.41988385821726837
1103	1	0.11228550924749925
1103	2	0.07824108748236114
1103	3	0.10722057084260683
1103	4	0.07656019241073153
1103	5	0.11674023117220855
1103	6	0.11820493803049459
1103	7	0.1111963181167468
1103	8	0.13164240980177766
1103	9	0.18485088136863936
1103	10-11	0.1721564506121922
1103	12-13	0.17225774855621268
1103	14-15	0.1874763051132149
1103	16-17	0.28247641693170067
1103	18-19	0.3027218424040825
1103	20-21	0.29450825827019855
1103	22-23	0.2721535199833127
1103	24-25	0.26278277292785646
1103	26-27	0.21722878364418108
1103	28-29	0.23990909958699547
1103	30-31	0.25213739710453353
1103	32-33	0.21032569599614703
1103	34-35	0.20106907883021563
1103	36-37	0.23279556488142816
1103	38-39	0.244974152267865
1103	40-41	0.25497360614658504
1103	42-43	0.24887112015296253
1103	44-45	0.25131108457186
1103	46-47	0.2921938184490287
1103	48-49	0.26092280368514764
1103	50-51	0.23184792118448172
1103	52-53	0.23701979485612412
1103	54-55	0.19731577753736218
1103	56-57	0.19252635862275014
1103	58-59	0.22356474963324757
1103	60-61	0.2669823330086487
1103	62-63	0.2301028211089431
1103	64-65	0.2396779410391261
1103	66-67	0.2586178130384482
1103	68-69	0.22420688103775888
1103	70-71	0.23821033590702712
1103	72-73	0.28292854987400773
1103	74-75	0.2881313491493387
1103	76-77	0.3063751630596414
1103	78-79	0.30595710067400006
1103	80-81	0.2517488168842519
1103	82-83	0.2989955555026498
1103	84-85	0.33475195369775435
1103	86-87	0.26039957306679895
1103	88-89	0.25655628206993697
1103	90-91	0.29264764767159335
1103	92-93	0.34943886698414417
1103	94-95	0.28762247773985905
1103	96-97	0.3261912348662577
1103	98-99	0.32069568923879643
1103	100	0.3650418479664239
1104	1	0.10216767431460738
1104	2	0.09255832081795745
1104	3	0.13116545158368353
1104	4	0.10628386601565865
1104	5	0.07775298114906093
1104	6	0.11341692745492082
1104	7	0.1310913048043716
1104	8	0.13024437903253272
1104	9	0.13639869659429138
1104	10-11	0.18404515488339257
1104	12-13	0.19012405345499417
1104	14-15	0.22892214448118864
1104	16-17	0.2435476705906794
1104	18-19	0.22325044287075713
1104	20-21	0.21807760412615806
1104	22-23	0.22940117429082107
1104	24-25	0.27379816912029753
1104	26-27	0.2297620190417291
1104	28-29	0.28815530312160575
1104	30-31	0.30439524754034153
1104	32-33	0.27962265845491174
1104	34-35	0.25923663237383465
1104	36-37	0.30920111185943
1104	38-39	0.25113567854307917
1104	40-41	0.28751536272271494
1104	42-43	0.33977134721283875
1104	44-45	0.3520152102041507
1104	46-47	0.32308968358002943
1104	48-49	0.2510318100047755
1104	50-51	0.29581496819416486
1104	52-53	0.3426488533640395
1104	54-55	0.3379025624095604
1104	56-57	0.3261948041140954
1104	58-59	0.2767148666201109
1104	60-61	0.34734349406966913
1104	62-63	0.34447012986168346
1104	64-65	0.33107103144683236
1104	66-67	0.32932933942070264
1104	68-69	0.35233563814582425
1104	70-71	0.3013848504551291
1104	72-73	0.3118338323443197
1104	74-75	0.2183525796049821
1104	76-77	0.25154868443429734
1104	78-79	0.21901745219800972
1104	80-81	0.27280605470976127
1104	82-83	0.2733127692535149
1104	84-85	0.28032806648720765
1104	86-87	0.26845351924605154
1104	88-89	0.2966312246263385
1104	90-91	0.32484267485406093
1104	92-93	0.28014105054014493
1104	94-95	0.3465464572491257
1104	96-97	0.3592340758214547
1104	98-99	0.3410988768491521
1104	100	0.28659162120943193
1105	1	0.05932658119966305
1105	2	0.10371292089646289
1105	3	0.10223191715712687
1105	4	0.03824013308990715
1105	5	0.02698044209385131
1105	6	0.08141020315883907
1105	7	0.07295273553548043
1105	8	0.04375898521392685
1105	9	0.10493268853471704
1105	10-11	0.12661021934270167
1105	12-13	0.1503849555049399
1105	14-15	0.15718026639446947
1105	16-17	0.17551622275161805
1105	18-19	0.15478866137671332
1105	20-21	0.16625821403933116
1105	22-23	0.15225465551475992
1105	24-25	0.12528910124995463
1105	26-27	0.1669487803133336
1105	28-29	0.1890882455362899
1105	30-31	0.16973382161981476
1105	32-33	0.16578177114750048
1105	34-35	0.21204887083754187
1105	36-37	0.2429497623049386
1105	38-39	0.17131095698726284
1105	40-41	0.1910916031840202
1105	42-43	0.18548599322809167
1105	44-45	0.20041725126139198
1105	46-47	0.18605755922064304
1105	48-49	0.21520299048889058
1105	50-51	0.19018685531214885
1105	52-53	0.19671181926113945
1105	54-55	0.199505489484487
1105	56-57	0.19726128908504847
1105	58-59	0.1989539534165985
1105	60-61	0.20747943092467125
1105	62-63	0.16970231422833137
1105	64-65	0.19875306124661307
1105	66-67	0.2014586568123562
1105	68-69	0.20835215913039917
1105	70-71	0.18769352868994105
1105	72-73	0.21076139600320687
1105	74-75	0.2044920383141431
1105	76-77	0.1718045379601989
1105	78-79	0.1505419415807694
1105	80-81	0.15711569070716536
1105	82-83	0.1864745132607979
1105	84-85	0.15337407764995703
1105	86-87	0.1681101704706549
1105	88-89	0.15901523535755047
1105	90-91	0.16560850000163185
1105	92-93	0.18762078030875529
1105	94-95	0.1564927824122222
1105	96-97	0.15441829597676104
1105	98-99	0.22222969278430327
1105	100	0.2562546772747538
1106	1	-0.026082680115450785
1106	2	-0.04691660510277984
1106	3	0.0128916707921789
1106	4	0.02296792512012047
1106	5	0.012767577439412037
1106	6	0.025034506818286673
1106	7	0.047921898675696184
1106	8	0.035114701294645556
1106	9	0.04609132964184681
1106	10-11	0.10039976643122372
1106	12-13	0.01894043155468239
1106	14-15	0.07151694398088893
1106	16-17	0.07807643139368281
1106	18-19	0.0671775065836826
1106	20-21	0.0826796360693649
1106	22-23	0.09322004563124864
1106	24-25	0.07175164549015989
1106	26-27	0.0795248230622363
1106	28-29	0.030571351494067756
1106	30-31	0.053828163627699155
1106	32-33	0.02500996480820561
1106	34-35	0.04534387587057864
1106	36-37	0.019559293671228772
1106	38-39	0.05655157514216569
1106	40-41	0.04518886105546471
1106	42-43	0.04370018829376221
1106	44-45	0.05506809854821171
1106	46-47	0.035709115363246724
1106	48-49	0.012613263883451964
1106	50-51	-0.025178006151463705
1106	52-53	-0.0022500336813351396
1106	54-55	0.046971515856206736
1106	56-57	-0.022850864475977062
1106	58-59	-0.031405004454342134
1106	60-61	0.03338687044775668
1106	62-63	0.00798744346302982
1106	64-65	0.039144055642246656
1106	66-67	-0.01355782000128869
1106	68-69	-0.003335839630899784
1106	70-71	0.032194702466917136
1106	72-73	0.06254183674491287
1106	74-75	0.031359628300855036
1106	76-77	0.044680737033033324
1106	78-79	0.05904457480033187
1106	80-81	0.03203006452209678
1106	82-83	0.05879678604843264
1106	84-85	0.013795402988137084
1106	86-87	-0.0519783715779667
1106	88-89	-0.0014429169984211399
1106	90-91	0.0050501543926273484
1106	92-93	0.013307845716049371
1106	94-95	0.0015311104403039622
1106	96-97	0.04815200474777015
1106	98-99	0.07526223783951735
1106	100	0.027195342779265275
1107	1	0.005531667945334107
1107	2	0.008094810518556983
1107	3	0.030041015467141108
1107	4	0.029363279947226317
1107	5	0.050196821036252004
1107	6	0.045474642787965536
1107	7	0.029926763075970086
1107	8	-0.0015652994671242482
1107	9	0.015991350465640153
1107	10-11	0.047501520781040085
1107	12-13	0.05870310276948487
1107	14-15	0.08006472802661335
1107	16-17	0.06860662089643199
1107	18-19	0.10143227318706494
1107	20-21	0.07650920238814507
1107	22-23	0.10209893811148874
1107	24-25	0.11295929896727586
1107	26-27	0.11900251350704849
1107	28-29	0.14843526130299978
1107	30-31	0.13640023439612037
1107	32-33	0.1500048330079693
1107	34-35	0.14974443945542504
1107	36-37	0.10176605050772736
1107	38-39	0.1033522664795683
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2216	42-43	-0.3629844993551501
2216	44-45	-0.4183376326989148
2216	46-47	-0.3808240454218321
2216	48-49	-0.3996208817956841
2216	50-51	-0.46706499830337833
2216	52-53	-0.4368004103508909
2216	54-55	-0.41112490025349757
2216	56-57	-0.3990474146411884
2216	58-59	-0.38807679088116487
2216	60-61	-0.4052572519437305
2216	62-63	-0.4508969633326032
2216	64-65	-0.4973120113795275
2216	66-67	-0.5087403559295822
2216	68-69	-0.4652314147268015
2216	70-71	-0.4559653482427777
2216	72-73	-0.44045960654619165
2216	74-75	-0.5281249175374754
2216	76-77	-0.4801699186196018
2216	78-79	-0.48653384692543256
2216	80-81	-0.45262477495415965
2216	82-83	-0.4279514239888229
2216	84-85	-0.5057107990741159
2216	86-87	-0.5083477881447465
2216	88-89	-0.6705006839268535
2216	90-91	-0.6645891337965537
2216	92-93	-0.6478867525899474
2216	94-95	-0.5073482592993059
2216	96-97	-0.6457307805896093
2216	98-99	-0.7300789709641577
2216	100	-0.7080372619846429
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35.0
3	14.0
4	20.0
5	43.0
6	114.0
7	273.0
8	604.0
9	1086.0
10	1896.0
11	2798.0
12	4522.0
13	6527.0
14	7540.0
15	9820.0
16	11998.0
17	16737.0
18	19632.0
19	11097.0
20	8192.0
21	8295.0
22	8849.0
23	9553.0
24	11150.0
25	13815.0
26	17760.0
27	22838.0
28	29307.0
29	37947.0
30	49076.0
31	62446.0
32	80196.0
33	104049.0
34	146787.0
35	226990.0
36	438130.0
37	979530.0
38	1674501.0
39	727965.0
40	1683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04020280814188	19.28823955638455	13.480907712067491	24.190649923406085
2	15.48574096261312	20.54403763780278	39.75729082717726	24.212930572406847
3	17.785302243862542	25.929795833878526	30.544664573410312	25.740237348848616
4	12.41365150781433	17.05191027960945	38.38829190386777	32.14614630870846
5	12.906797817748098	38.06401398458708	35.481198745005315	13.5479894526595
6	30.66895816197955	37.937464896577694	17.873263761067438	13.520313180375322
7	27.16045954670091	32.85479136230585	22.751874021180882	17.23287506981235
8	25.672378170044247	35.54320145702447	20.51902499853801	18.265395374393275
9	26.130423670252206	15.823817292006526	20.598067026167406	37.447692011573864
10-11	24.303534946417514	26.15193302525647	28.9337228048682	20.61080922345782
12-13	25.243704266993984	23.68104354081932	29.065603099826138	22.009649092360554
14-15	22.5840834961241	25.302329093884126	26.17853311712742	25.93505429286435
16-17	21.925040491235812	28.587175350952972	26.80392018480348	22.683863973007735
18-19	21.88185604924749	27.294311028547668	29.0503641781902	21.773468744014636
20-21	22.947432746120747	26.745140481907686	28.62103384334477	21.686392928626795
22-23	22.872478836435725	26.52388916924898	28.400627118433707	22.203004875881582
24-25	22.456980795578396	26.51000294714761	28.556861121611593	22.476155135662403
26-27	22.329644818614458	26.62213817736319	28.609564895361313	22.438652108661042
28-29	22.36436917374608	26.815319517733688	28.49371187922096	22.326599429299264
30-31	22.470882315076825	26.914884307583055	28.22297137539305	22.39126200194707
32-33	22.3994623265735	26.69140469286247	28.604289397042166	22.304843583521865
34-35	22.535563542123537	26.512054002942897	28.53002272911335	22.42235972582021
36-37	22.662745605371686	26.757351726981383	28.183700880240398	22.396201787406536
38-39	22.577887444084382	26.943486441941893	28.152641615207997	22.325984498765727
40-41	22.39773049502168	26.694366542050886	28.387607671485537	22.5202952914419
42-43	22.538718902607695	26.615255326511445	28.182165271471437	22.66386049940942
44-45	22.61152358684551	26.890266028442415	28.020021814058815	22.478188570653256
46-47	22.66855146866254	26.91160678318361	27.812409612069466	22.607432136084388
48-49	22.649440501996818	26.860763407915538	27.68615312123	22.803642968857645
50-51	22.61809725452084	27.071173362867505	27.49678942070737	22.813939961904282
52-53	22.659537655546124	27.026535529884942	27.54035443112532	22.773572383443614
54-55	22.7184272000488	27.05288278992767	27.442780167086855	22.78590984293667
56-57	22.463673912426124	26.964238196059377	27.618028888377022	22.95405900313748
58-59	22.46281144722712	26.648933540745695	27.845298986182677	23.042956025844504
60-61	22.689198044097214	27.067050358501536	27.42716113269027	22.816590464710973
62-63	22.48685529411641	26.913247570635374	27.54898960098363	23.050907534264585
64-65	22.597051000091504	26.618252918971397	27.65861734207158	23.126078738865523
66-67	22.430900234863998	26.84430294405651	27.524198985446425	23.200597835633065
68-69	22.59072367954667	26.915229764464364	27.41633382788475	23.077712728104217
70-71	22.56781844723189	26.75538281003241	27.49517834471209	23.181620398023608
72-73	22.502546648232283	26.84414933534716	27.51553889285356	23.137765123567
74-75	22.618960470831162	26.904989391657967	27.30282851256446	23.17322162494641
76-77	22.636808542191904	26.78860031364283	27.260431884707337	23.31415925945793
78-79	22.487086687218582	26.806806743636425	27.399109977986104	23.306996591158892
80-81	22.502295524752224	26.71111517802018	27.47512261204948	23.311466685178114
82-83	22.423457407562253	26.746560520172512	27.433704456901125	23.396277615364106
84-85	22.45495459962157	26.671567993285393	27.345016581419344	23.528460825673697
86-87	22.452985417322722	26.707884296974772	27.408125430840354	23.43100485486215
88-89	22.171939847390743	26.754025120174585	27.337769308133925	23.73626572430075
90-91	22.330211561075455	26.6382362407467	27.355549665419222	23.676002532758623
92-93	22.37875713773341	26.540146430686285	27.384505189023063	23.696591242557247
94-95	22.037083276974755	26.50904426302651	27.4445405315804	24.00933192841833
96-97	22.126928916377995	26.43486977004198	27.50172099072269	23.936480322857335
98-99	22.100976732758244	26.52440948393372	27.37145673531619	24.00315704799184
100	22.09875647243319	26.32054465729104	27.396775011227824	24.183923859047944
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	60.0
2	218.0
3	355.0
4	229.5
5	168.0
6	391.5
7	895.0
8	1547.5
9	1828.5
10	1398.0
11	945.5
12	973.5
13	1176.5
14	1420.0
15	1657.0
16	1897.0
17	2189.0
18	2514.5
19	2885.0
20	3344.0
21	3973.5
22	4960.5
23	6592.0
24	8677.5
25	11521.0
26	15511.0
27	21049.5
28	29453.5
29	40424.0
30	51908.0
31	64113.5
32	76651.0
33	92120.5
34	108672.0
35	121157.0
36	132709.5
37	146082.5
38	156763.5
39	162061.0
40	169167.5
41	175236.5
42	176193.5
43	176316.0
44	176458.5
45	174837.5
46	172982.5
47	169499.0
48	165946.0
49	163143.5
50	160557.5
51	158940.5
52	151301.0
53	145325.0
54	142066.5
55	131208.5
56	117307.5
57	105110.0
58	95388.0
59	89348.0
60	81593.0
61	71084.0
62	61403.0
63	51967.5
64	43586.5
65	36335.5
66	29012.0
67	21976.5
68	16877.0
69	12893.0
70	9096.0
71	6406.0
72	4588.5
73	3211.5
74	2377.5
75	1716.5
76	1087.0
77	747.0
78	475.5
79	263.5
80	138.0
81	64.5
82	23.0
83	13.0
84	11.0
85	4.5
86	1.0
87	2.5
88	3.5
89	2.5
90	1.5
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.044848190348172993
2	0.23873457423143307
3	0.002881895908864775
4	0.0023770382313994132
5	2.1035736561056753E-4
6	1.0517868280528376E-4
7	0.0
8	3.5760752153796475E-4
9	0.0
10-11	4.838219409043053E-4
12-13	0.0
14-15	2.8398244357426614E-4
16-17	1.7880376076898237E-4
18-19	6.205542285511742E-4
20-21	0.0
22-23	1.68285892488454E-4
24-25	0.0013883586130297456
26-27	9.466081452475539E-5
28-29	2.1035736561056753E-4
30-31	6.310720968317026E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	6.310720968317026E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.5242883873268103E-4
70-71	3.155360484158513E-5
72-73	3.6812538981849315E-4
74-75	1.8932162904951078E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.5776802420792562E-4
84-85	0.0
86-87	5.048576774653621E-4
88-89	0.0022297880754720157
90-91	0.002208752338910959
92-93	6.310720968317025E-4
94-95	5.89000623709589E-4
96-97	2.629467070132094E-4
98-99	1.4725015592739724E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4753815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.88258294672014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.49205348871843	23.29477816850528
2	15.928099855940328	12.067951279526012
3	7.424596687715589	8.437886996049883
4	4.2161092429213625	6.38868432429608
5	2.592121124478196	4.909812175299537
6	1.7602007560924715	4.000857068733154
7	1.2652198315828203	3.3550856651040037
8	0.921511165373117	2.792737852686068
9	0.7094791594607619	2.418921279651744
>10	3.4488867678317283	22.742918414090564
>50	0.1732612075996933	4.493327336152211
>100	0.0660123708387511	4.148444185322667
>500	0.00194754459520362	0.49096103981760775
>1k	3.895086623965923E-4	0.21374532186659587
>5k	1.1128818925597712E-4	0.24388889289875892
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6312	0.1327775691733902	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5258	0.1106059028380364	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.1035736561056753E-5	0.0
4	0.0	0.0	0.0	2.1035736561056753E-5	0.0
5	0.0	0.0	0.0	2.1035736561056753E-5	0.0
6	0.0	0.0	0.0	2.1035736561056753E-5	0.0
7	0.0	0.0	0.0	2.1035736561056753E-5	0.0
8	0.0	0.0	0.0	2.1035736561056753E-5	0.0
9	0.0	0.0	0.0	2.1035736561056753E-5	0.0
10-11	0.0	0.0	0.0	2.1035736561056753E-5	0.0
12-13	0.0	0.0	0.0	2.1035736561056753E-5	0.0
14-15	0.0	0.0	0.0	2.1035736561056753E-5	0.0
16-17	0.0	0.0	0.0	2.1035736561056753E-5	0.0
18-19	0.0	0.0	0.0	2.1035736561056753E-5	0.0
20-21	0.0	0.0	0.0	2.1035736561056753E-5	0.0
22-23	0.0	0.0	0.0	7.362507796369864E-5	0.0
24-25	0.0	0.0	0.0	2.5242883873268103E-4	0.0
26-27	0.0	0.0	0.0	4.627862043432485E-4	0.0
28-29	0.0	0.0	0.0	0.0013357692716271037	0.0
30-31	0.0	0.0	0.0	0.005921559841937475	0.0
32-33	0.0	0.0	0.0	0.017575357896762914	0.0
34-35	0.0	0.0	0.0	0.035245376608050584	0.0
36-37	0.0	0.0	0.0	0.06617842722108454	0.0
38-39	0.0	0.0	0.0	0.11448699623355138	0.0
40-41	0.0	0.0	0.0	0.16919042916057944	0.0
42-43	0.0	0.0	0.0	0.22890036738913905	0.0
44-45	0.0	0.0	0.0	0.30030617514564617	0.0
46-47	0.0	0.0	0.0	0.3845332643361174	0.0
48-49	0.0	0.0	0.0	0.4785524888957606	0.0
50-51	0.0	0.0	0.0	0.577336307786483	0.0
52-53	0.0	0.0	0.0	0.6795384338683773	0.0
54-55	0.0	0.0	0.0	0.792079624470031	0.0
56-57	0.0	0.0	0.0	0.928359643780837	0.0
58-59	0.0	0.0	0.0	1.07791321286167	0.0
60-61	0.0	0.0	0.0	1.2371537386288696	0.0
62-63	0.0	0.0	0.0	1.4023368599745678	0.0
64-65	0.0	0.0	0.0	1.5788687611949561	0.0
66-67	0.0	0.0	0.0	1.7783716867400183	0.0
68-69	0.0	0.0	0.0	2.0044532654299756	0.0
70-71	0.0	0.0	0.0	2.246763914876788	0.0
72-73	0.0	0.0	0.0	2.501348916606978	0.0
74-75	0.0	0.0	0.0	2.763496265630867	0.0
76-77	0.0	0.0	0.0	3.053011107920691	0.0
78-79	0.0	0.0	0.0	3.377981684184176	0.0
80-81	0.0	0.0	0.0	3.724934605153966	0.0
82-83	0.0	0.0	0.0	4.089336669601152	0.0
84-85	0.0	0.0	0.0	4.460070490753216	0.0
86-87	0.0	0.0	0.0	4.8566887857436605	0.0
88	0.0	0.0	0.0	5.174160121923129	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6895	0.0	47.089348	1
GTATCAA	12030	0.0	42.85381	1
TCAACGC	14400	0.0	35.15031	4
ATCAACG	14475	0.0	34.97002	3
CAACGCA	14615	0.0	34.633217	5
AACGCAG	15155	0.0	33.399174	6
TATCAAC	15330	0.0	33.334335	2
GTACATG	16870	0.0	29.581394	1
TACATGG	16990	0.0	28.441875	2
ACGCAGA	17780	0.0	28.309595	7
CGCAGAG	18145	0.0	27.455215	8
ACATGGG	17850	0.0	26.225267	3
GCAGAGT	20700	0.0	23.657743	9
CATGGGG	13495	0.0	23.403042	4
GAGTACT	11010	0.0	20.660175	12-13
AGAGTAC	18035	0.0	20.260967	10-11
GAGTACA	11625	0.0	20.106037	1
CAGAGTA	19680	0.0	19.403238	10-11
AGTACTT	11695	0.0	19.048206	12-13
AGTACAT	11970	0.0	18.807798	2
>>END_MODULE