FastQCFastQC Report
Wed 25 May 2016
SRR1033037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3106064
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41350.036.3820721
GGTATCA26250.036.1772041
TCAACGC44300.032.6832244
ATCAACG44500.032.5358123
CAACGCA45700.031.6830125
AACGCAG48300.029.8763266
GTACATG100650.028.1654361
TATCAAC52900.027.9026152
TACATGG102000.027.0986772
ACATGGG107300.025.1468223
ACGCAGA58100.024.5141287
CGCAGAG61250.022.5830948
CATGGGG80500.021.8400524
GAGTACA70800.019.787731
TAAGTCG1951.1384964E-519.27007394
GTATAGA11600.019.048151
GTATTAG13950.018.8723831
GCAGAGT71150.018.2523359
AGTACAT75550.017.3596382
TATACTG20000.017.1587165
Produced by FastQC (version 0.11.5)