FastQCFastQC Report
Wed 25 May 2016
SRR1033037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3106064
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54410.1751734671275286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45320.14590813325160074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42050.054.2425351
GTATCAA76900.042.529841
ATCAACG91150.035.0098763
TCAACGC91350.034.933794
CAACGCA93200.034.240365
AACGCAG96150.033.0920646
TATCAAC98900.032.9119152
ACGCAGA114000.027.7451887
GTACATG100800.027.06941
CGCAGAG118100.026.7426138
TACATGG105800.024.888432
GCAGAGT131050.023.7054889
ACATGGG108350.023.2928643
CAGAGTA124000.021.03547310-11
GTGGTAT17750.020.974391
CATGGGG81000.020.8882454
AGAGTAC117950.020.60030210-11
CCGATCG1605.4179523E-520.5618659
GAGTACT80750.020.28358712-13
GAGTACA73500.019.6839221
Produced by FastQC (version 0.11.5)