##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033037_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3106064 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69212031690268 34.0 31.0 34.0 31.0 34.0 2 32.69198767314518 34.0 31.0 34.0 31.0 34.0 3 32.799847652849394 34.0 31.0 34.0 31.0 34.0 4 36.29369613761983 37.0 37.0 37.0 35.0 37.0 5 36.27514532862169 37.0 37.0 37.0 35.0 37.0 6 36.342776259600576 37.0 37.0 37.0 35.0 37.0 7 36.29316652844243 37.0 37.0 37.0 35.0 37.0 8 36.30493866192068 37.0 37.0 37.0 35.0 37.0 9 38.1086381349515 39.0 39.0 39.0 37.0 39.0 10-11 38.067933725769976 39.0 39.0 39.0 35.0 39.0 12-13 38.0366515306832 39.0 39.0 39.0 35.0 39.0 14-15 39.531931409011534 41.0 39.0 41.0 37.0 41.0 16-17 39.52251434613066 41.0 39.0 41.0 37.0 41.0 18-19 39.532496432784384 41.0 39.5 41.0 37.0 41.0 20-21 39.52038335333721 41.0 39.5 41.0 37.0 41.0 22-23 39.45214007180792 41.0 39.0 41.0 36.0 41.0 24-25 39.39192431321441 41.0 39.0 41.0 36.0 41.0 26-27 39.27942453858002 41.0 39.0 41.0 36.0 41.0 28-29 39.227291034569795 41.0 39.0 41.0 36.0 41.0 30-31 39.225241656321316 41.0 39.0 41.0 36.0 41.0 32-33 39.19579586898402 41.0 39.0 41.0 35.0 41.0 34-35 39.11528094720521 41.0 39.0 41.0 35.0 41.0 36-37 39.03453760128575 41.0 39.0 41.0 35.0 41.0 38-39 38.947347028264716 41.0 39.0 41.0 35.0 41.0 40-41 38.846366655677414 41.0 39.0 41.0 35.0 41.0 42-43 38.72406589175239 41.0 38.5 41.0 35.0 41.0 44-45 38.55448084778678 40.5 38.0 41.0 35.0 41.0 46-47 38.35267286829891 40.0 38.0 41.0 34.5 41.0 48-49 38.142882760947614 40.0 38.0 41.0 34.0 41.0 50-51 37.9895340211921 40.0 38.0 41.0 34.0 41.0 52-53 37.83907656120415 40.0 37.0 41.0 33.5 41.0 54-55 37.67097442293527 40.0 37.0 41.0 33.0 41.0 56-57 37.4918235747879 40.0 36.5 41.0 33.0 41.0 58-59 37.28894333793508 40.0 36.0 41.0 33.0 41.0 60-61 37.07158802909406 39.0 35.5 41.0 33.0 41.0 62-63 36.81817164746123 39.0 35.0 41.0 32.0 41.0 64-65 36.530863337007865 39.0 35.0 41.0 32.0 41.0 66-67 36.23390277856477 38.0 35.0 40.5 32.0 41.0 68-69 35.9127187978097 37.0 35.0 40.0 31.0 41.0 70-71 35.579542952109165 37.0 35.0 39.5 31.0 41.0 72-73 35.23320881346939 36.0 35.0 39.0 31.0 41.0 74-75 34.89297033158364 36.0 35.0 39.0 31.0 40.5 76-77 34.55168164596738 35.0 34.5 37.5 30.5 39.5 78-79 34.21417781475205 35.0 34.0 37.0 30.0 39.0 80-81 33.91003936171309 35.0 34.0 37.0 30.0 39.0 82-83 33.636852138268885 35.0 34.0 36.0 30.0 38.0 84-85 33.44411705618429 35.0 34.0 36.0 30.0 37.0 86-87 33.2641350918719 35.0 34.0 35.5 30.0 37.0 88-89 33.07880906510619 35.0 34.0 35.0 30.0 36.5 90-91 32.91664804717482 35.0 34.0 35.0 30.0 36.0 92-93 32.75620624687707 35.0 34.0 35.0 29.0 36.0 94-95 32.61159734635217 35.0 34.0 35.0 29.0 36.0 96-97 32.47566164122826 35.0 34.0 35.0 29.0 35.5 98-99 32.35351171128477 35.0 34.0 35.0 29.0 35.0 100 32.25111845731447 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11934190227761121 1101 2 0.1181776156267702 1101 3 0.1629285352184624 1101 4 0.11318231195836859 1101 5 0.09463628271308266 1101 6 0.07802542962807735 1101 7 0.10824240230880378 1101 8 0.13764015019204834 1101 9 0.16461310845632227 1101 10-11 0.1915583632318416 1101 12-13 0.17965674147416166 1101 14-15 0.2479248139350929 1101 16-17 0.29395634731743314 1101 18-19 0.2702980550062506 1101 20-21 0.25346739711851285 1101 22-23 0.24079116041268378 1101 24-25 0.2866963360370107 1101 26-27 0.29711187289332486 1101 28-29 0.2909727196803473 1101 30-31 0.28591269547992937 1101 32-33 0.26166369270865175 1101 34-35 0.24565007576117637 1101 36-37 0.24982181456385888 1101 38-39 0.22602021967499297 1101 40-41 0.23143474424663424 1101 42-43 0.2081616012529679 1101 44-45 0.2690723858318691 1101 46-47 0.2526235507361534 1101 48-49 0.257562004212609 1101 50-51 0.23940015533635517 1101 52-53 0.21799007182826102 1101 54-55 0.249428851192512 1101 56-57 0.23010575059985428 1101 58-59 0.2709363187657061 1101 60-61 0.1704104698242972 1101 62-63 0.20252712650911064 1101 64-65 0.15772084166250977 1101 66-67 0.12824120565547048 1101 68-69 0.16736616434168639 1101 70-71 0.1178892594565113 1101 72-73 0.139911129026018 1101 74-75 0.06438434776201518 1101 76-77 0.08909041863334721 1101 78-79 0.10048831470404451 1101 80-81 0.12810228317088246 1101 82-83 0.13723743093093077 1101 84-85 0.18754857567753902 1101 86-87 0.23318060217907544 1101 88-89 0.22992933752298228 1101 90-91 0.2886551990064419 1101 92-93 0.19312347418053832 1101 94-95 0.16119339913108632 1101 96-97 0.15479737318390363 1101 98-99 0.22843241936053005 1101 100 0.16442932684545752 1102 1 0.10854557301307466 1102 2 0.0651310519731183 1102 3 0.08292731247241392 1102 4 0.08166255760074392 1102 5 0.044589569809019736 1102 6 0.09058849734947927 1102 7 0.09924850437699462 1102 8 0.0975266586265846 1102 9 0.14946289622506725 1102 10-11 0.143660025730604 1102 12-13 0.20316924676183135 1102 14-15 0.23999752044105804 1102 16-17 0.22186524896753923 1102 18-19 0.21863138029003437 1102 20-21 0.18490397411752468 1102 22-23 0.16980059240238177 1102 24-25 0.20669587897971553 1102 26-27 0.19705602473341344 1102 28-29 0.1646381683521696 1102 30-31 0.166349748266164 1102 32-33 0.17240632363171216 1102 34-35 0.19975080435525427 1102 36-37 0.16680012637093 1102 38-39 0.1713914780043524 1102 40-41 0.13752563927671702 1102 42-43 0.19071423440358615 1102 44-45 0.2709392822679888 1102 46-47 0.2322174152839267 1102 48-49 0.27273586444660936 1102 50-51 0.3317837432572759 1102 52-53 0.36709264337628156 1102 54-55 0.2521686622605799 1102 56-57 0.2605201743383745 1102 58-59 0.2914619900126354 1102 60-61 0.2601146182906362 1102 62-63 0.22702336515144594 1102 64-65 0.2967704499302499 1102 66-67 0.21692654633218922 1102 68-69 0.2587089750955869 1102 70-71 0.20856586209314543 1102 72-73 0.20689443547974662 1102 74-75 0.25370445619768134 1102 76-77 0.25555848723293195 1102 78-79 0.2547458269058893 1102 80-81 0.2612539676449188 1102 82-83 0.22672910076735064 1102 84-85 0.2589911356507031 1102 86-87 0.22038196736679083 1102 88-89 0.26928986254515763 1102 90-91 0.20417075079297575 1102 92-93 0.2273183978054405 1102 94-95 0.22590849989160233 1102 96-97 0.24996262000838954 1102 98-99 0.2384185910215919 1102 100 0.2160211407609438 1103 1 0.08448189186516686 1103 2 0.08884921856539307 1103 3 0.10418382293351414 1103 4 0.09364244302300051 1103 5 0.09915396780721153 1103 6 0.10106193930917584 1103 7 0.09810116855300066 1103 8 0.09051245721053647 1103 9 0.11464884959953991 1103 10-11 0.17532701772126558 1103 12-13 0.14781642151632468 1103 14-15 0.21623157899664847 1103 16-17 0.19065038339005014 1103 18-19 0.19644542145557864 1103 20-21 0.22827293270310633 1103 22-23 0.21716367081365462 1103 24-25 0.18215877179706297 1103 26-27 0.19346278710447962 1103 28-29 0.19304611917955583 1103 30-31 0.1923604252631108 1103 32-33 0.11282294991725195 1103 34-35 0.13521556721681804 1103 36-37 0.16981272892192578 1103 38-39 0.20871273749505548 1103 40-41 0.10371989796799141 1103 42-43 0.11389886964714435 1103 44-45 0.11773664955514107 1103 46-47 0.12772340556789175 1103 48-49 0.04866225295443627 1103 50-51 0.08286129367225925 1103 52-53 0.09822171896929177 1103 54-55 0.15532829760650912 1103 56-57 0.15831523410016501 1103 58-59 0.16594280818200957 1103 60-61 0.16480006297668837 1103 62-63 0.16442608076503973 1103 64-65 0.19045067305246022 1103 66-67 0.20958008219449908 1103 68-69 0.13934288140865192 1103 70-71 0.22162218887131502 1103 72-73 0.21117907190630092 1103 74-75 0.20947014716536927 1103 76-77 0.20428392811519558 1103 78-79 0.11921184169607812 1103 80-81 0.1425940886472432 1103 82-83 0.14316886532377993 1103 84-85 0.1340150230767705 1103 86-87 0.14128592216904678 1103 88-89 0.12242137897811034 1103 90-91 0.07319326366830126 1103 92-93 0.12958188498744505 1103 94-95 0.1446033796559476 1103 96-97 0.11757659943062038 1103 98-99 0.15700028988622705 1103 100 0.20445848904735442 1104 1 0.09854012511760146 1104 2 0.08332155884173886 1104 3 0.12819815217185493 1104 4 0.06398824313129126 1104 5 0.079655614505306 1104 6 0.0810855263279393 1104 7 0.11204264617076376 1104 8 0.09268369000720611 1104 9 0.0839297279481741 1104 10-11 0.16283210887289812 1104 12-13 0.18162737628958325 1104 14-15 0.15918189758045997 1104 16-17 0.1677295155764682 1104 18-19 0.24418884559212017 1104 20-21 0.2304983502857425 1104 22-23 0.22951596249195916 1104 24-25 0.2352614310554344 1104 26-27 0.25119103625473116 1104 28-29 0.27895759871475434 1104 30-31 0.25012167762798043 1104 32-33 0.2941375794090959 1104 34-35 0.24980386455824544 1104 36-37 0.24413461565835348 1104 38-39 0.23574161281908346 1104 40-41 0.26437059259367857 1104 42-43 0.29553233183368377 1104 44-45 0.28014498177393676 1104 46-47 0.17795424411789185 1104 48-49 0.11909024729822448 1104 50-51 0.1962447572654895 1104 52-53 0.1742893405001098 1104 54-55 0.08311840019131722 1104 56-57 0.12441982355964853 1104 58-59 0.08029348778853063 1104 60-61 0.1511330260247732 1104 62-63 0.10339237030775905 1104 64-65 0.13814817943777058 1104 66-67 0.12289659795531804 1104 68-69 0.13080051328840625 1104 70-71 0.13127732259168567 1104 72-73 0.1430277312832544 1104 74-75 0.07481305489358192 1104 76-77 0.08462311784191456 1104 78-79 0.0595905335655047 1104 80-81 0.11691250193059233 1104 82-83 0.11407158115289917 1104 84-85 0.12131955207004808 1104 86-87 0.14246884394688664 1104 88-89 0.1120619945302721 1104 90-91 0.11385473898341303 1104 92-93 0.12174916713701123 1104 94-95 0.11856011016922707 1104 96-97 0.19487421289783668 1104 98-99 0.23324869869845344 1104 100 0.2688234141397956 1105 1 0.00641160695906251 1105 2 0.06376961578516216 1105 3 0.07434885216532194 1105 4 0.0666001873713924 1105 5 0.05241049674783227 1105 6 0.08174266598234681 1105 7 0.06755241988602023 1105 8 0.04596231691299835 1105 9 0.09032462237047412 1105 10-11 0.10436898597091471 1105 12-13 0.11558627683213274 1105 14-15 0.1531752103960642 1105 16-17 0.12755005363410987 1105 18-19 0.11527327524360942 1105 20-21 0.10177399911563612 1105 22-23 0.16704039049841413 1105 24-25 0.19292594595509627 1105 26-27 0.21903778010851482 1105 28-29 0.20150367822931514 1105 30-31 0.15849615462122557 1105 32-33 0.20951952907758198 1105 34-35 0.20834896710638873 1105 36-37 0.15501814569258698 1105 38-39 0.14109386274937208 1105 40-41 0.1366042602759734 1105 42-43 0.1329052151799317 1105 44-45 0.18533378007344936 1105 46-47 0.11285319024331386 1105 48-49 0.1728161188889814 1105 50-51 0.25282173808645325 1105 52-53 0.2299146866970716 1105 54-55 0.2660281591997702 1105 56-57 0.22171431063227232 1105 58-59 0.22703510580943487 1105 60-61 0.2669586975148448 1105 62-63 0.2761469756439965 1105 64-65 0.2356120152196155 1105 66-67 0.2168001110859592 1105 68-69 0.2073597700555112 1105 70-71 0.13496340685058072 1105 72-73 0.14874335918725023 1105 74-75 0.14398173400235947 1105 76-77 0.15226086410775963 1105 78-79 0.12022672987401961 1105 80-81 0.12177446363116928 1105 82-83 0.1563576846311392 1105 84-85 0.13089425374710117 1105 86-87 0.10659348008432801 1105 88-89 0.0695914150546244 1105 90-91 0.12153729894311027 1105 92-93 0.18210317542225596 1105 94-95 0.09326525881098746 1105 96-97 0.11022752327666296 1105 98-99 0.0958356690677391 1105 100 0.1668693377150916 1106 1 -0.041258285704344644 1106 2 0.01921769906309123 1106 3 0.005855243388083409 1106 4 0.005882969663908 1106 5 0.020557110952474034 1106 6 0.009581931413947586 1106 7 0.050197109780668825 1106 8 0.06340231147779463 1106 9 0.050786603041821365 1106 10-11 0.10104141599243377 1106 12-13 0.04270757683096349 1106 14-15 0.07646655948398262 1106 16-17 0.10950283300212504 1106 18-19 0.10239839855332633 1106 20-21 0.10786449581597424 1106 22-23 0.09943969754858273 1106 24-25 0.07455304621388592 1106 26-27 0.0720236098001763 1106 28-29 0.08643222138620388 1106 30-31 0.09164388463985773 1106 32-33 0.06501747995424978 1106 34-35 0.0781213256561557 1106 36-37 0.031120763818506703 1106 38-39 0.02804463230496168 1106 40-41 0.05220635667826201 1106 42-43 0.017970841305547935 1106 44-45 0.009331349436997982 1106 46-47 -0.013547098569191007 1106 48-49 0.044562948538590774 1106 50-51 -0.05651586873264591 1106 52-53 -0.07652108201696706 1106 54-55 -0.0352149846068599 1106 56-57 -0.06260368163005836 1106 58-59 -0.08910641616469661 1106 60-61 -0.061305477003664066 1106 62-63 -0.08640552751796804 1106 64-65 -0.03028756421618084 1106 66-67 -0.05526971760219368 1106 68-69 -0.08444026260574589 1106 70-71 -0.04249172313669902 1106 72-73 -0.031888616875534126 1106 74-75 5.168770425996172E-4 1106 76-77 0.014287072600701833 1106 78-79 0.021467256160995873 1106 80-81 3.7775129359829407E-4 1106 82-83 -0.026269837414005792 1106 84-85 0.023407060106364952 1106 86-87 -0.04616968240677011 1106 88-89 0.02812463498776907 1106 90-91 -0.01634690025513663 1106 92-93 0.01821158481041607 1106 94-95 0.0442774883054895 1106 96-97 0.03362799983845832 1106 98-99 0.004309932257605453 1106 100 0.015621016608562854 1107 1 -0.009521414496795444 1107 2 0.014166453022077974 1107 3 0.03480308921777464 1107 4 0.05449677241436035 1107 5 0.057321103584719424 1107 6 0.07094085594496136 1107 7 0.01811132429300244 1107 8 0.06031042374396378 1107 9 0.04887824060292445 1107 10-11 0.03803904202948871 1107 12-13 0.060595659394095946 1107 14-15 0.08751851772760944 1107 16-17 0.08304973343068411 1107 18-19 0.09469893798798523 1107 20-21 0.061845943299367434 1107 22-23 0.10735735542645841 1107 24-25 0.12145493963920728 1107 26-27 0.08293301765436922 1107 28-29 0.11441748586913292 1107 30-31 0.10992743695710772 1107 32-33 0.08405363332400384 1107 34-35 0.040537576730152125 1107 36-37 0.0823689792492246 1107 38-39 0.12323322706528472 1107 40-41 0.08794894418389276 1107 42-43 0.10779143427031102 1107 44-45 0.10807365582447659 1107 46-47 0.1624823301667675 1107 48-49 0.19312400672941266 1107 50-51 0.2730636529380064 1107 52-53 0.22947868717409392 1107 54-55 0.2343124617677148 1107 56-57 0.20070158215521872 1107 58-59 0.23291749998197986 1107 60-61 0.2647862065178117 1107 62-63 0.2546390235071385 1107 64-65 0.21487594231648188 1107 66-67 0.23367468028423843 1107 68-69 0.23370226828257756 1107 70-71 0.2089387819233579 1107 72-73 0.18689741412015337 1107 74-75 0.19578710639170538 1107 76-77 0.16713320282226363 1107 78-79 0.13137836724971663 1107 80-81 0.13159856628722366 1107 82-83 0.12399795727150575 1107 84-85 0.1758265004821027 1107 86-87 0.15617054728613056 1107 88-89 0.15055298865391364 1107 90-91 0.15531684269419088 1107 92-93 0.19588279210829995 1107 94-95 0.16895661831964048 1107 96-97 0.14352917726164094 1107 98-99 0.1322552927024887 1107 100 0.10267023132941233 1108 1 -0.018618074845647925 1108 2 -0.007802739476069576 1108 3 0.003959573455084353 1108 4 -0.0319923533817672 1108 5 -0.033124007895622753 1108 6 -0.0376893267422318 1108 7 -0.008237017288784898 1108 8 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2214 56-57 -0.25517474723138633 2214 58-59 -0.21776064107606885 2214 60-61 -0.23106349644780266 2214 62-63 -0.27669733595131873 2214 64-65 -0.307058364036493 2214 66-67 -0.2850071421076876 2214 68-69 -0.3425135231414558 2214 70-71 -0.21951544435402326 2214 72-73 -0.2913626093611086 2214 74-75 -0.26571666544720784 2214 76-77 -0.24713723609137617 2214 78-79 -0.17055275853056884 2214 80-81 -0.21546726109629333 2214 82-83 -0.2578095634932609 2214 84-85 -0.22244972305480104 2214 86-87 -0.2154178150053525 2214 88-89 -0.2003858461847443 2214 90-91 -0.16413944260171576 2214 92-93 -0.20152406719590488 2214 94-95 -0.22542007374156015 2214 96-97 -0.14865032884030427 2214 98-99 -0.1858821828744297 2214 100 -0.1544327974365629 2215 1 -0.19085321879582295 2215 2 -0.13693494038131604 2215 3 -0.15279177439089153 2215 4 -0.13401237810237632 2215 5 -0.1758412330771506 2215 6 -0.12544829273633695 2215 7 -0.2141604891608324 2215 8 -0.14160380384811333 2215 9 -0.24778077716165114 2215 10-11 -0.19797963914858485 2215 12-13 -0.2197476384031134 2215 14-15 -0.26076741815366944 2215 16-17 -0.3009492197727326 2215 18-19 -0.311596241487635 2215 20-21 -0.3343704615624574 2215 22-23 -0.3280160312440046 2215 24-25 -0.42272780358711515 2215 26-27 -0.4167378159292241 2215 28-29 -0.468164449466002 2215 30-31 -0.4942882695429205 2215 32-33 -0.4114126668022067 2215 34-35 -0.399576780014101 2215 36-37 -0.35984572298266215 2215 38-39 -0.4288203184824937 2215 40-41 -0.454913421464461 2215 42-43 -0.40639625941597046 2215 44-45 -0.42919812218898556 2215 46-47 -0.4354995646544282 2215 48-49 -0.36099489160485376 2215 50-51 -0.3896975285916682 2215 52-53 -0.3454367875459212 2215 54-55 -0.3210502069890637 2215 56-57 -0.2638649131168478 2215 58-59 -0.3877905314398191 2215 60-61 -0.3598948208350663 2215 62-63 -0.29854534960310275 2215 64-65 -0.34372403627007486 2215 66-67 -0.38800124626691 2215 68-69 -0.32407536317414554 2215 70-71 -0.335366771507843 2215 72-73 -0.39749768779701355 2215 74-75 -0.38077627087400856 2215 76-77 -0.36285466537985656 2215 78-79 -0.3394476246422684 2215 80-81 -0.34455321772499303 2215 82-83 -0.3397717265892126 2215 84-85 -0.2698218698764663 2215 86-87 -0.2848904403660697 2215 88-89 -0.23979857607568533 2215 90-91 -0.23430179655232308 2215 92-93 -0.18168739008130785 2215 94-95 -0.15072170250822126 2215 96-97 -0.2582569986815031 2215 98-99 -0.2942131416800393 2215 100 -0.3003350000421072 2216 1 -0.18909195474614648 2216 2 -0.13674205753881807 2216 3 -0.12955131891937555 2216 4 -0.11522269570387067 2216 5 -0.1341075050840601 2216 6 -0.15994586050326376 2216 7 -0.13760965892856802 2216 8 -0.18598345989644116 2216 9 -0.2001627883610695 2216 10-11 -0.25216827418081067 2216 12-13 -0.2452682975608127 2216 14-15 -0.2817169674019411 2216 16-17 -0.28134644102649276 2216 18-19 -0.3289611523452862 2216 20-21 -0.30715693379278264 2216 22-23 -0.3439076978513782 2216 24-25 -0.3713925064419925 2216 26-27 -0.36278659340894137 2216 28-29 -0.36957110674052274 2216 30-31 -0.32755940364422287 2216 32-33 -0.31410860920654216 2216 34-35 -0.37259615842497595 2216 36-37 -0.363166829927394 2216 38-39 -0.30282305548587374 2216 40-41 -0.2556218641949215 2216 42-43 -0.2882227288950645 2216 44-45 -0.31376100211423363 2216 46-47 -0.21875495624276198 2216 48-49 -0.16210137384398138 2216 50-51 -0.20239702269667248 2216 52-53 -0.17970568864143388 2216 54-55 -0.21349113254209584 2216 56-57 -0.1904109109016332 2216 58-59 -0.11405654373973562 2216 60-61 -0.1321241385527756 2216 62-63 -0.19743222645455205 2216 64-65 -0.26367110466432564 2216 66-67 -0.2454146950571925 2216 68-69 -0.18770790505338653 2216 70-71 -0.2466500916779495 2216 72-73 -0.20422813091170156 2216 74-75 -0.2836950098601818 2216 76-77 -0.2481013810943793 2216 78-79 -0.23083857498012605 2216 80-81 -0.2200819408441319 2216 82-83 -0.24431235946788377 2216 84-85 -0.313973837601651 2216 86-87 -0.28299185179650266 2216 88-89 -0.4504855188618748 2216 90-91 -0.5272607723343512 2216 92-93 -0.4809707351548269 2216 94-95 -0.3817352831679912 2216 96-97 -0.4751095515182442 2216 98-99 -0.5538986709012228 2216 100 -0.5729857190391385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18.0 3 3.0 4 10.0 5 30.0 6 64.0 7 179.0 8 404.0 9 705.0 10 1299.0 11 1952.0 12 2968.0 13 4396.0 14 5114.0 15 6656.0 16 8566.0 17 12579.0 18 14810.0 19 8453.0 20 6008.0 21 5819.0 22 6203.0 23 6885.0 24 7685.0 25 9585.0 26 12000.0 27 15842.0 28 20127.0 29 25807.0 30 33133.0 31 42559.0 32 54366.0 33 71190.0 34 100091.0 35 157101.0 36 302145.0 37 667391.0 38 1059270.0 39 433453.0 40 1198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45225518186784 19.096026891085437 13.312130878881892 24.139587048164827 2 15.923593396606389 19.994945987255885 39.455928750684194 24.62553186545353 3 18.239162182837216 25.311496289733082 29.99037983551708 26.458961691912624 4 12.602025063289265 16.63766344696049 37.79206993483854 32.96824155491171 5 13.046343629127339 37.86809518688962 35.30858084427271 13.776980339710335 6 31.43907991504352 37.519224509756896 17.38752072158274 13.65417485361685 7 27.902483657773953 32.383395834728454 22.159749444956702 17.554371062540888 8 26.049611500637145 35.723622319508934 19.767396188218235 18.45936999163569 9 26.069359807138554 15.741143775530702 20.352027517784567 37.83746889954618 10-11 24.740626578576023 26.113981680227866 28.35343876436997 20.791952976826142 12-13 25.452260481432447 23.50131871075419 28.695834986014457 22.35058582179891 14-15 22.537362902884382 25.12904228530366 25.905799171972298 26.427795639839662 16-17 22.055038233336845 28.657247013015528 26.461216609214443 22.826498144433188 18-19 22.02755911849455 27.32726453212279 28.791825630624107 21.853350718758552 20-21 22.937872497153954 26.47675643515394 28.670996476569705 21.9143745911224 22-23 22.724439555076064 26.22284380303143 28.670849459073377 22.38186718281913 24-25 22.475947936629563 26.403801002729377 28.608646589005716 22.511604471635348 26-27 22.274886322971703 26.58486631851911 28.62439648190786 22.515850876601327 28-29 22.373489462760727 26.499655270165007 28.702545720770285 22.424309546303984 30-31 22.403416287247325 26.69932216420664 28.49906086871415 22.398200679831888 32-33 22.40937405024494 26.53425686012909 28.706056926064626 22.35031216356134 34-35 22.54274219719877 26.377740445786053 28.648492110915935 22.431025246099242 36-37 22.66851230367436 26.561767561775934 28.280148123155218 22.489572011394486 38-39 22.45356180683978 26.83980433114063 28.32076222511835 22.385871636901236 40-41 22.451564070517588 26.588055872677362 28.443991137330805 22.51638891947424 42-43 22.49422420143307 26.57580461960861 28.293122743124417 22.636848435833905 44-45 22.50138760823988 26.775542937943325 28.191627732075062 22.53144172174173 46-47 22.633306976288964 26.863161866593867 27.92598607111766 22.577545085999514 48-49 22.68770057539059 26.89724036594223 27.61889323594105 22.796165822726124 50-51 22.650016226323732 26.956205667365513 27.537085520452898 22.856692585857857 52-53 22.71654737313848 26.921660339258946 27.596372772743898 22.765419514858674 54-55 22.661059141086596 27.240745844258203 27.422583694347573 22.67561132030763 56-57 22.507070041055176 27.046915324346184 27.56940938757218 22.876605247026465 58-59 22.52989635757666 26.73571761560612 27.77085404550582 22.963531981311398 60-61 22.715774691055948 26.977631497612414 27.5553723297395 22.751221481592136 62-63 22.49874761112456 26.9395447099609 27.662533676060765 22.899174002853773 64-65 22.546026096049534 26.830580438780398 27.65904050914598 22.964352956024086 66-67 22.372272814126305 26.917398823301586 27.657869840716394 23.05245852185572 68-69 22.62084805522882 26.952316914727486 27.53744736699345 22.88938766305024 70-71 22.61976012720927 26.859983200598315 27.51397830629965 23.006278365892772 72-73 22.579093043044608 26.925117600868237 27.492927528374402 23.002861827712756 74-75 22.589019529564787 26.94957599016117 27.431086328016846 23.030318152257202 76-77 22.60273774139876 26.84196140195437 27.393012507147308 23.162288349499562 78-79 22.669284985756892 27.004450004893656 27.35175450344874 22.974510505900717 80-81 22.566325098259405 27.002341226710076 27.344703779445627 23.086629895584895 82-83 22.491541537658147 26.954187313127463 27.4648340776308 23.08943707158359 84-85 22.507520772269984 26.929805696212313 27.292177495376784 23.270496036140916 86-87 22.613755339575768 26.955376149530014 27.307157124220716 23.123711386673502 88-89 22.408058359454884 26.987786832676967 27.30567239065651 23.298482417211634 90-91 22.419013822632568 26.89817716910502 27.311507141194458 23.371301867067956 92-93 22.453155117643025 26.93455576521813 27.278304366737295 23.333984750401555 94-95 22.326980090317242 26.788206827864897 27.371616343128892 23.513196738688976 96-97 22.422624568705597 26.772528643228377 27.337956128944192 23.466890659121837 98-99 22.275217200443198 26.949065206252488 27.19654919681996 23.579168396484356 100 22.362031175146424 26.647293809786277 27.32113697592838 23.669538039138924 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 42.0 1 39.5 2 152.0 3 293.5 4 210.0 5 130.5 6 337.5 7 802.0 8 1436.0 9 1744.0 10 1396.0 11 1030.0 12 1056.0 13 1223.5 14 1416.0 15 1638.5 16 1854.5 17 2069.5 18 2361.0 19 2669.0 20 3121.5 21 3782.5 22 4647.5 23 6103.0 24 8025.5 25 10359.5 26 13827.5 27 18271.5 28 24032.5 29 31188.0 30 37775.0 31 44781.5 32 52358.0 33 59869.5 34 67946.0 35 74869.0 36 80668.0 37 87043.5 38 92901.5 39 96496.5 40 100482.0 41 105167.5 42 108080.0 43 110813.0 44 111827.5 45 111576.0 46 111793.0 47 109101.0 48 106936.0 49 105737.0 50 104309.0 51 103911.0 52 99753.5 53 95350.0 54 92569.0 55 88150.5 56 81354.5 57 73096.5 58 66508.0 59 62126.5 60 56886.0 61 49627.0 62 43189.5 63 36873.5 64 30420.5 65 24958.0 66 20098.5 67 15089.0 68 11180.0 69 8626.5 70 6098.5 71 4287.0 72 3195.5 73 2271.5 74 1619.5 75 1115.5 76 762.5 77 536.5 78 311.0 79 162.5 80 88.0 81 32.5 82 14.5 83 10.0 84 5.0 85 3.5 86 4.5 87 3.0 88 1.0 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04481556078689943 2 0.24262217391528315 3 0.0028331676359534125 4 0.0028009725491812145 5 1.9317052063318722E-4 6 9.658526031659361E-5 7 0.0 8 3.219508677219787E-4 9 0.0 10-11 6.760968222161552E-4 12-13 0.0 14-15 2.7365823756368186E-4 16-17 1.6097543386098934E-4 18-19 4.5073121481077014E-4 20-21 0.0 22-23 1.6097543386098934E-4 24-25 0.0013360961010462115 26-27 1.7707297724708828E-4 28-29 2.7365823756368186E-4 30-31 6.439017354439573E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.439017354439573E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.6097543386098933E-5 68-69 1.448778904748904E-4 70-71 3.2195086772197865E-5 72-73 2.2536560740538507E-4 74-75 1.2878034708879146E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.658526031659361E-5 84-85 0.0 86-87 3.8634104126637444E-4 88-89 0.0023180462475982464 90-91 0.002285851160826049 92-93 4.829263015829681E-4 94-95 4.024385846524733E-4 96-97 2.2536560740538507E-4 98-99 1.448778904748904E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3106064.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.81426318726808 #Duplication Level Percentage of deduplicated Percentage of total 1 70.95549762877393 36.76506828720869 2 15.319445831380648 15.875315963805079 3 5.543631093418909 8.617174814625901 4 2.66886227462003 5.531405292309328 5 1.4788087802912968 3.8311693672828087 6 0.9119906199108577 2.835247320262858 7 0.6054180583336762 2.1958503428978142 8 0.4144000347565494 1.7177465972551116 9 0.3198627589439886 1.4916107858156562 >10 1.6669045667786073 15.295258085538228 >50 0.0866385314266744 3.035856083745193 >100 0.027980302206787832 2.26220188270707 >500 3.1084397677254234E-4 0.10845882501079884 >1k 1.8650638591191925E-4 0.2623692097200141 >5k 6.216879530397309E-5 0.17526714181542005 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5441 0.1751734671275286 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4532 0.14590813325160074 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 3.2195086772197865E-5 0.0 0.0 0.0 16-17 0.0 3.2195086772197865E-5 0.0 0.0 0.0 18-19 0.0 3.2195086772197865E-5 0.0 0.0 0.0 20-21 0.0 3.2195086772197865E-5 0.0 1.6097543386098933E-5 0.0 22-23 0.0 3.2195086772197865E-5 0.0 9.65852603165936E-5 0.0 24-25 0.0 3.2195086772197865E-5 0.0 1.448778904748904E-4 0.0 26-27 0.0 3.2195086772197865E-5 0.0 2.7365823756368186E-4 0.0 28-29 0.0 3.2195086772197865E-5 0.0 9.175599730076392E-4 0.0 30-31 0.0 3.2195086772197865E-5 0.0 0.0037346300655749527 0.0 32-33 0.0 3.2195086772197865E-5 0.0 0.010399013027419912 0.0 34-35 0.0 3.2195086772197865E-5 0.0 0.020202416949554165 0.0 36-37 0.0 3.2195086772197865E-5 0.0 0.03910093288483431 0.0 38-39 0.0 3.2195086772197865E-5 0.0 0.07176284841522904 0.0 40-41 0.0 3.2195086772197865E-5 0.0 0.10914134415775077 0.0 42-43 0.0 3.2195086772197865E-5 0.0 0.14843544756321828 0.0 44-45 0.0 3.2195086772197865E-5 0.0 0.19391100762894775 0.0 46-47 0.0 3.2195086772197865E-5 0.0 0.24859436251152583 0.0 48-49 0.0 3.2195086772197865E-5 0.0 0.31197039082259737 0.0 50-51 0.0 3.2195086772197865E-5 0.0 0.38041714530029 0.0 52-53 0.0 3.2195086772197865E-5 0.0 0.45018389833564276 0.0 54-55 0.0 3.2195086772197865E-5 0.0 0.5258262547069217 0.0 56-57 0.0 3.2195086772197865E-5 0.0 0.6182261537431295 0.0 58-59 0.0 3.2195086772197865E-5 0.0 0.7221196987570121 0.0 60-61 0.0 3.2195086772197865E-5 0.0 0.8387142055025267 0.0 62-63 0.0 3.2195086772197865E-5 0.0 0.9560652967871879 0.0 64-65 0.0 3.2195086772197865E-5 0.0 1.075042239953845 0.0 66-67 0.0 3.2195086772197865E-5 0.0 1.2155576961711025 0.0 68-69 0.0 3.2195086772197865E-5 0.0 1.3771448366807637 0.0 70-71 0.0 3.2195086772197865E-5 0.0 1.5517065971596207 0.0 72-73 0.0 3.2195086772197865E-5 0.0 1.7348966408934265 0.0 74-75 0.0 3.2195086772197865E-5 0.0 1.9238818002462281 0.0 76-77 0.0 3.2195086772197865E-5 0.0 2.1301235261089273 0.0 78-79 0.0 3.2195086772197865E-5 0.0 2.364648635701003 0.0 80-81 0.0 3.2195086772197865E-5 0.0 2.622499085659536 0.0 82-83 0.0 3.2195086772197865E-5 0.0 2.8956904944650206 0.0 84-85 0.0 3.2195086772197865E-5 0.0 3.1615897161166027 0.0 86-87 0.0 3.2195086772197865E-5 0.0 3.445405503556913 0.0 88 0.0 3.2195086772197865E-5 0.0 3.6763891536040467 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4205 0.0 54.242535 1 GTATCAA 7690 0.0 42.52984 1 ATCAACG 9115 0.0 35.009876 3 TCAACGC 9135 0.0 34.93379 4 CAACGCA 9320 0.0 34.24036 5 AACGCAG 9615 0.0 33.092064 6 TATCAAC 9890 0.0 32.911915 2 ACGCAGA 11400 0.0 27.745188 7 GTACATG 10080 0.0 27.0694 1 CGCAGAG 11810 0.0 26.742613 8 TACATGG 10580 0.0 24.88843 2 GCAGAGT 13105 0.0 23.705488 9 ACATGGG 10835 0.0 23.292864 3 CAGAGTA 12400 0.0 21.035473 10-11 GTGGTAT 1775 0.0 20.97439 1 CATGGGG 8100 0.0 20.888245 4 AGAGTAC 11795 0.0 20.600302 10-11 CCGATCG 160 5.4179523E-5 20.561865 9 GAGTACT 8075 0.0 20.283587 12-13 GAGTACA 7350 0.0 19.683922 1 >>END_MODULE