FastQCFastQC Report
Wed 25 May 2016
SRR1033036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461379
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32950.035.9560851
TCAACGC31250.035.800044
ATCAACG31750.035.5323643
GGTATCA19850.035.290111
CAACGCA33050.033.8509565
AACGCAG35550.031.4621166
TATCAAC40500.028.7841422
GTACATG67950.026.9145681
ACGCAGA42350.026.1889467
CGCAGAG42100.026.1467298
TACATGG67950.025.526632
ACATGGG68250.024.381323
TATACCG1354.074591E-420.8921415
CCGGTCG1605.3757933E-520.5824479
GCAGAGT53050.020.0420449
CTGTCGC6350.020.003649
CATGGGG43250.019.9980814
TAGTCGC1456.607665E-419.446166
TTAGTCG2201.4873913E-619.2302635
ATACCGC1751.07925356E-418.7979556
Produced by FastQC (version 0.11.5)