##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033036_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2461379
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62029658983846	33.0	31.0	34.0	30.0	34.0
2	31.673880373562948	33.0	31.0	34.0	30.0	34.0
3	31.721714941096028	34.0	31.0	34.0	30.0	34.0
4	35.08628374581891	37.0	35.0	37.0	33.0	37.0
5	35.134535965408006	37.0	35.0	37.0	33.0	37.0
6	35.19756323589338	37.0	35.0	37.0	33.0	37.0
7	35.07044465724295	37.0	35.0	37.0	33.0	37.0
8	35.06791964991982	37.0	35.0	37.0	33.0	37.0
9	36.67698026187759	39.0	37.0	39.0	33.0	39.0
10-11	36.68946736768291	39.0	37.5	39.0	33.0	39.0
12-13	36.65794418494673	39.0	37.5	39.0	33.0	39.0
14-15	38.05301052783825	41.0	39.0	41.0	33.0	41.0
16-17	38.07036177687386	41.0	38.5	41.0	33.0	41.0
18-19	38.086638628183636	41.0	38.5	41.0	33.0	41.0
20-21	38.11119539087642	41.0	39.0	41.0	33.5	41.0
22-23	38.084727707516805	40.0	39.0	41.0	33.0	41.0
24-25	38.108829643870365	40.0	38.5	41.0	33.0	41.0
26-27	38.03150774423606	40.0	38.0	41.0	33.0	41.0
28-29	38.05092592404502	40.0	38.0	41.0	33.0	41.0
30-31	38.20401124735362	40.5	39.0	41.0	33.0	41.0
32-33	38.18845573964838	41.0	39.0	41.0	33.0	41.0
34-35	38.149927337480335	41.0	38.0	41.0	33.0	41.0
36-37	38.07792643879711	41.0	38.0	41.0	33.0	41.0
38-39	37.99706688811435	40.5	38.0	41.0	33.0	41.0
40-41	37.930918196669424	40.0	38.0	41.0	33.0	41.0
42-43	37.82178100162551	40.0	38.0	41.0	32.5	41.0
44-45	37.710857206468404	40.0	38.0	41.0	32.0	41.0
46-47	37.593436646692766	40.0	38.0	41.0	32.0	41.0
48-49	37.492603333334685	40.0	38.0	41.0	31.5	41.0
50-51	37.38668222163267	40.0	37.5	41.0	31.0	41.0
52-53	37.24615611817603	40.0	37.0	41.0	31.0	41.0
54-55	37.1023300353176	40.0	37.0	41.0	31.0	41.0
56-57	36.947728285648004	40.0	36.5	41.0	30.5	41.0
58-59	36.76989443722401	40.0	36.0	41.0	30.5	41.0
60-61	36.56552404160432	39.5	36.0	41.0	30.0	41.0
62-63	36.32489795354555	39.0	35.0	41.0	30.0	41.0
64-65	36.058550105449015	39.0	35.0	41.0	30.0	41.0
66-67	35.765527779346456	38.5	35.0	40.5	29.5	41.0
68-69	35.451405086335754	38.0	35.0	40.0	29.0	41.0
70-71	35.11872206596384	37.0	35.0	40.0	29.0	41.0
72-73	34.763800698714014	37.0	34.0	39.5	29.0	41.0
74-75	34.405225688526635	36.0	34.0	39.0	28.0	41.0
76-77	34.03375485855693	36.0	34.0	39.0	28.0	40.0
78-79	33.63799276746897	35.0	34.0	37.5	27.5	39.5
80-81	33.27383592693364	35.0	34.0	37.0	27.0	39.0
82-83	33.032401145861726	35.0	34.0	37.0	27.0	39.0
84-85	32.791766119723945	35.0	34.0	36.0	27.0	38.0
86-87	32.59332512384317	35.0	34.0	36.0	27.0	37.0
88-89	32.357287723670346	35.0	34.0	36.0	27.0	37.0
90-91	32.13424060252403	35.0	33.5	35.0	26.5	36.5
92-93	31.909541358726145	35.0	33.0	35.0	26.0	36.0
94-95	31.706399339557215	35.0	33.0	35.0	26.0	36.0
96-97	31.532178709577032	35.0	33.0	35.0	25.0	36.0
98-99	31.358831167406564	35.0	33.0	35.0	25.0	35.5
100	31.21506968248287	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24601428496938382
1101	2	0.2699336943623081
1101	3	0.2666489768920677
1101	4	0.13521791183880794
1101	5	0.16309971255569167
1101	6	0.26639499608425155
1101	7	0.27574591478554566
1101	8	0.3301370347122443
1101	9	0.3081496059707547
1101	10-11	0.2949488958629587
1101	12-13	0.2949285287627461
1101	14-15	0.3989596972796079
1101	16-17	0.4108162112692071
1101	18-19	0.4175906092608628
1101	20-21	0.40668602047502844
1101	22-23	0.3490832559897683
1101	24-25	0.38252723531761745
1101	26-27	0.3802466106223008
1101	28-29	0.3855549596622083
1101	30-31	0.412431648381947
1101	32-33	0.36527522899090314
1101	34-35	0.4022999915322103
1101	36-37	0.3798752334830624
1101	38-39	0.42135166875833363
1101	40-41	0.4674852995090504
1101	42-43	0.4731541333668403
1101	44-45	0.39585420605589405
1101	46-47	0.39771769188377704
1101	48-49	0.4265506990719956
1101	50-51	0.4724511981206376
1101	52-53	0.3588372078565669
1101	54-55	0.39660069022846045
1101	56-57	0.4803372422701173
1101	58-59	0.4915630098254553
1101	60-61	0.4886827639221778
1101	62-63	0.4133306275373485
1101	64-65	0.38190373741414874
1101	66-67	0.4226529329484521
1101	68-69	0.34923788269215805
1101	70-71	0.36486131128148713
1101	72-73	0.3468267913786036
1101	74-75	0.3245007409889027
1101	76-77	0.29446320173867946
1101	78-79	0.30631132047181353
1101	80-81	0.45038369397420297
1101	82-83	0.4068090893357166
1101	84-85	0.30931864430497313
1101	86-87	0.2748894462103664
1101	88-89	0.2997640938875179
1101	90-91	0.31113745435362006
1101	92-93	0.24986883873619803
1101	94-95	0.2541993371896538
1101	96-97	0.26932003880858346
1101	98-99	0.2764778784394366
1101	100	0.22611002806719682
1102	1	0.047129868505674466
1102	2	0.11987115390424208
1102	3	0.09557499781023182
1102	4	-0.07958329274318032
1102	5	-0.025199461969720005
1102	6	0.11416119064416108
1102	7	0.1312787572586558
1102	8	0.21665861841649559
1102	9	0.11769217250034814
1102	10-11	0.18474892990161607
1102	12-13	0.16392893678071374
1102	14-15	0.2155880456838304
1102	16-17	0.2208078576774284
1102	18-19	0.16309386485773558
1102	20-21	0.20448989538222406
1102	22-23	0.18082703483065643
1102	24-25	0.13185872501424
1102	26-27	-0.028594540330225016
1102	28-29	0.0629589769669181
1102	30-31	0.09287099602885718
1102	32-33	0.1233477974751267
1102	34-35	0.16110823721105305
1102	36-37	0.21022801666349977
1102	38-39	0.26826550339139743
1102	40-41	0.20682443675543993
1102	42-43	0.22638176725655512
1102	44-45	0.22641369232486142
1102	46-47	0.18081684842369583
1102	48-49	0.20517039065797604
1102	50-51	0.23022351294997634
1102	52-53	0.1796862010778142
1102	54-55	0.22904586606636457
1102	56-57	0.20630078523351614
1102	58-59	0.19058278197455536
1102	60-61	0.1881962080768531
1102	62-63	0.2660949492279272
1102	64-65	0.07130563807923807
1102	66-67	0.1273910879789284
1102	68-69	0.1672042736060675
1102	70-71	0.07226756190856776
1102	72-73	0.1379860677672866
1102	74-75	0.2000420708301789
1102	76-77	0.1234654008737337
1102	78-79	0.1296353625400286
1102	80-81	0.1218428375526841
1102	82-83	0.030353992492763382
1102	84-85	0.012588324854398536
1102	86-87	0.0608111914867564
1102	88-89	0.06684752352745704
1102	90-91	0.12458852081149985
1102	92-93	0.1212129346271098
1102	94-95	0.22428758872492693
1102	96-97	0.19902798267304433
1102	98-99	0.23575030957989895
1102	100	0.1186927966288529
1103	1	-0.17936938844255224
1103	2	-0.070205963012274
1103	3	-0.10785752506532376
1103	4	-0.1969490836761736
1103	5	-0.12596445382916954
1103	6	-0.15522259115920178
1103	7	-0.08831966816741499
1103	8	-0.07209847217191623
1103	9	-0.08324599692965506
1103	10-11	-0.043899248396726875
1103	12-13	-0.03896857461712244
1103	14-15	-0.01996442555795852
1103	16-17	-0.04031164762689343
1103	18-19	0.020562298470515827
1103	20-21	0.03376869138983807
1103	22-23	-0.017072982991223284
1103	24-25	-0.04301884717681048
1103	26-27	-0.20266737644488586
1103	28-29	-0.1604500295448119
1103	30-31	-0.09015751330206712
1103	32-33	-0.21737294854460032
1103	34-35	-0.21974538123452447
1103	36-37	-0.16567257415673708
1103	38-39	-0.11494143777012056
1103	40-41	-0.031484124635262845
1103	42-43	-0.12804034934399766
1103	44-45	-0.08891885973313407
1103	46-47	-0.10071217319757864
1103	48-49	-0.10732503849081354
1103	50-51	-0.11660708544633991
1103	52-53	-0.03690229663693856
1103	54-55	-0.018196978732028413
1103	56-57	-0.0015243700334153232
1103	58-59	-0.05871685711458241
1103	60-61	-0.08398555594865087
1103	62-63	-0.013619932611575791
1103	64-65	0.026976191714936704
1103	66-67	0.001563897041251039
1103	68-69	-0.06472337949348628
1103	70-71	-0.08753108277635135
1103	72-73	-0.022050882461371657
1103	74-75	0.03859019807699582
1103	76-77	0.038251776151447814
1103	78-79	0.006124838754203665
1103	80-81	0.036617142233019706
1103	82-83	-0.09602711460821922
1103	84-85	-0.09462946635408542
1103	86-87	-0.12364457722923561
1103	88-89	-0.11421764988797634
1103	90-91	-0.14114603539169224
1103	92-93	-0.07257003319196897
1103	94-95	-0.08748040942329993
1103	96-97	-0.12470668145548558
1103	98-99	-0.1759506967529063
1103	100	-0.07574890629102171
1104	1	-0.027746489420032105
1104	2	0.04829559470564604
1104	3	0.10892572215217555
1104	4	0.06763246836392511
1104	5	-0.018047042004440073
1104	6	0.07130802655144208
1104	7	0.15613608802378565
1104	8	0.18894549008933126
1104	9	0.22629210438863367
1104	10-11	0.21906658558997805
1104	12-13	0.2378946106799944
1104	14-15	0.23217304262983873
1104	16-17	0.19343425386426816
1104	18-19	0.2728299112619439
1104	20-21	0.20112067470989814
1104	22-23	0.274390167087347
1104	24-25	0.26416970465103873
1104	26-27	0.11737962960989279
1104	28-29	0.06909592564460354
1104	30-31	0.2154159063323391
1104	32-33	0.2060509710124805
1104	34-35	0.22588296528121532
1104	36-37	0.2564271345202229
1104	38-39	0.2105008362770917
1104	40-41	0.3373721294364671
1104	42-43	0.3561831953966177
1104	44-45	0.34473643433172896
1104	46-47	0.33487704456890555
1104	48-49	0.3352524004776427
1104	50-51	0.34360574240925956
1104	52-53	0.30075616174123354
1104	54-55	0.28712644609142757
1104	56-57	0.4242210614306785
1104	58-59	0.3393014130117109
1104	60-61	0.3644195993511161
1104	62-63	0.3548868141516337
1104	64-65	0.34832196558906503
1104	66-67	0.41389807356654273
1104	68-69	0.3768442880005267
1104	70-71	0.30416073681091405
1104	72-73	0.3581994270400699
1104	74-75	0.3451563992472444
1104	76-77	0.34586516013878565
1104	78-79	0.4292061139336312
1104	80-81	0.3570755893626725
1104	82-83	0.405691965879555
1104	84-85	0.3299365453323162
1104	86-87	0.31899287053716563
1104	88-89	0.3404332746659833
1104	90-91	0.4025613767745142
1104	92-93	0.3684031538393029
1104	94-95	0.40054837939996446
1104	96-97	0.5168989330245637
1104	98-99	0.5507366803420481
1104	100	0.6491779616481104
1105	1	-0.09953478284458583
1105	2	-0.05542992625124654
1105	3	-0.04331817418958295
1105	4	-0.023872981627022227
1105	5	-0.02474601577866764
1105	6	-0.07409404579708934
1105	7	-0.027007777452126902
1105	8	0.003937237938288263
1105	9	0.015588669095954799
1105	10-11	0.027541908214644195
1105	12-13	0.053842785385818104
1105	14-15	0.02295980934268016
1105	16-17	0.033504824423346236
1105	18-19	0.06812698232227632
1105	20-21	0.048296449353472326
1105	22-23	-0.03559799675164754
1105	24-25	-0.018262396854247243
1105	26-27	-0.030769120833326724
1105	28-29	0.07111559816048185
1105	30-31	0.06390268248349429
1105	32-33	0.05722208771855719
1105	34-35	0.006468376957691646
1105	36-37	0.12255718816557959
1105	38-39	0.13429024444352677
1105	40-41	0.17239718561996398
1105	42-43	0.12869725083780992
1105	44-45	0.11263910614101036
1105	46-47	0.08466579843032207
1105	48-49	0.052379312693631164
1105	50-51	0.01735385793426758
1105	52-53	0.11764928426845245
1105	54-55	0.06326022404606846
1105	56-57	0.0931414453916588
1105	58-59	0.06434806253635372
1105	60-61	0.03668245523309821
1105	62-63	0.05378390204787564
1105	64-65	0.0788409264047445
1105	66-67	0.07621354807160685
1105	68-69	0.12628117753904178
1105	70-71	0.05939980047875082
1105	72-73	0.08697134243192295
1105	74-75	0.055291462853858775
1105	76-77	-0.003131635934870758
1105	78-79	-0.040216924235437546
1105	80-81	0.06633760855051918
1105	82-83	0.019525893571838537
1105	84-85	0.0655483715448213
1105	86-87	0.020637752545432875
1105	88-89	0.03227537925785384
1105	90-91	0.007402436774157195
1105	92-93	0.04862401048145415
1105	94-95	0.014078590945832303
1105	96-97	0.0995907158208027
1105	98-99	0.1078483892133022
1105	100	0.05831310560796865
1106	1	0.12065141152416814
1106	2	0.12395900632377632
1106	3	0.060918364893250754
1106	4	0.1281942360945152
1106	5	0.06960003326765474
1106	6	0.17889271425630682
1106	7	0.14733083479178077
1106	8	0.11666026581410449
1106	9	0.1800145418534811
1106	10-11	0.19213701394525629
1106	12-13	0.20457023572077304
1106	14-15	0.2823744506998338
1106	16-17	0.23323615469662684
1106	18-19	0.29117474549060063
1106	20-21	0.2688883151984385
1106	22-23	0.2980483528146394
1106	24-25	0.18161919340232657
1106	26-27	0.16404035682273843
1106	28-29	0.21264465350369477
1106	30-31	0.21094632493178977
1106	32-33	0.19362767385440094
1106	34-35	0.21975015557261912
1106	36-37	0.18297198801196402
1106	38-39	0.2444056672745134
1106	40-41	0.26578627973209024
1106	42-43	0.2864230675145407
1106	44-45	0.3095193827656786
1106	46-47	0.28723568608583605
1106	48-49	0.2910595240938889
1106	50-51	0.2895200247502814
1106	52-53	0.3115230658824828
1106	54-55	0.25849449856161044
1106	56-57	0.32096575398238514
1106	58-59	0.2694754177777696
1106	60-61	0.3320223384387262
1106	62-63	0.2766309629869923
1106	64-65	0.2738164367705451
1106	66-67	0.2959797459758846
1106	68-69	0.22780511246092772
1106	70-71	0.18716703770665077
1106	72-73	0.15523547388045245
1106	74-75	0.25020433949437404
1106	76-77	0.2604947805626381
1106	78-79	0.1732820913025961
1106	80-81	0.2770146775969593
1106	82-83	0.20957223643281253
1106	84-85	0.2521186188489821
1106	86-87	0.2714023106957413
1106	88-89	0.21289854672610886
1106	90-91	0.18032097026109994
1106	92-93	0.20655472020843035
1106	94-95	0.246038096947089
1106	96-97	0.3199817270430607
1106	98-99	0.33394912740737936
1106	100	0.31060967553524677
1107	1	0.055597043897410714
1107	2	0.04385338658854465
1107	3	0.059001478124383056
1107	4	0.10059030423713011
1107	5	0.02620232966120284
1107	6	0.16082446754538182
1107	7	0.11712298940359034
1107	8	0.1329499547680868
1107	9	0.12001816318532832
1107	10-11	0.19786637876804036
1107	12-13	0.24606893929863105
1107	14-15	0.2586546171638062
1107	16-17	0.1479172465794889
1107	18-19	0.26448908515833125
1107	20-21	0.24196290657071984
1107	22-23	0.2083415120894614
1107	24-25	0.1363913977011535
1107	26-27	0.21107273402184035
1107	28-29	0.20922898252002398
1107	30-31	0.19706793717217863
1107	32-33	0.1469215097464982
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2216	50-51	-0.7090095191150567
2216	52-53	-0.7914234826803437
2216	54-55	-0.842611301125693
2216	56-57	-0.9182058400377429
2216	58-59	-0.8511025136672856
2216	60-61	-0.7867995059561324
2216	62-63	-0.844241376726508
2216	64-65	-0.8079119032764339
2216	66-67	-0.8221686494124469
2216	68-69	-0.8335487017539265
2216	70-71	-0.868343818910148
2216	72-73	-0.7893913477138312
2216	74-75	-0.7907014128715488
2216	76-77	-0.6837041230608492
2216	78-79	-0.7523443152021514
2216	80-81	-0.6617981604705605
2216	82-83	-0.7986473876146647
2216	84-85	-0.6491587942332089
2216	86-87	-0.64968776021
2216	88-89	-0.76679195030691
2216	90-91	-0.7406795657738954
2216	92-93	-0.9523385583354376
2216	94-95	-0.8523528293249072
2216	96-97	-0.7769392714967864
2216	98-99	-0.7257828433520004
2216	100	-0.6690526987416483
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28628.0
3	5585.0
4	2988.0
5	2623.0
6	2198.0
7	2304.0
8	2771.0
9	3117.0
10	3458.0
11	3862.0
12	4373.0
13	4913.0
14	5643.0
15	6046.0
16	6016.0
17	5971.0
18	5722.0
19	5273.0
20	5458.0
21	5503.0
22	6321.0
23	9603.0
24	15032.0
25	19876.0
26	19128.0
27	16015.0
28	17829.0
29	22053.0
30	27064.0
31	32245.0
32	42324.0
33	56148.0
34	79890.0
35	121596.0
36	216921.0
37	462375.0
38	805445.0
39	378106.0
40	956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.52738242215782	20.505748042283344	13.448943261889498	23.51792627366934
2	15.07091845464954	20.464232687804756	41.24711780100562	23.217731056540078
3	17.58603462076148	26.180736423914286	30.46493675326803	25.7682922020562
4	11.644472778605396	17.123311063550197	40.675517658615284	30.556698499229125
5	12.01405007473963	38.828376987025074	36.53100759432727	12.626565343908029
6	29.74681047891104	39.607071333448545	18.24326769612378	12.402850491516636
7	26.18626065202858	33.264298808209	23.493914172179398	17.055526367583024
8	24.160028435907165	37.492694794301265	21.003528866797378	17.34374790299419
9	24.34333329266656	17.31509835258905	21.898853603686035	36.442714751058354
10-11	22.8580201412224	27.71745352318884	29.84398432217822	19.580542013410536
12-13	23.58854892273254	25.25749957143563	29.637132565787933	21.516818940043898
14-15	20.997438523340023	27.377662849803436	26.638909879783856	24.985988747072685
16-17	20.4666666802194	30.653456546651114	27.214301440296385	21.6655753328331
18-19	20.307737166790396	29.663704261296804	29.208393800441364	20.820164771471443
20-21	21.798189334817312	28.927619953467694	28.573737730269972	20.70045298144502
22-23	21.345882425633906	28.645316732737342	28.70545920474538	21.30334163688337
24-25	21.241415995516956	28.75935610264354	28.66058849321793	21.338639408621574
26-27	20.933226940244243	28.643998914904458	28.862116957914697	21.560657186936606
28-29	20.965943300764998	28.676520509596298	28.934046576259142	21.423489613379566
30-31	21.32333956910176	29.25213064456649	28.43752325198915	20.987006534342605
32-33	20.857016122999262	28.81146547882738	29.082689324083017	21.248829074090338
34-35	21.024417252081335	28.503705509755218	29.12157830841829	21.350298929745158
36-37	21.422855861826893	28.973565220713933	28.430004225483508	21.173574691975666
38-39	21.175840859228757	29.160933812778815	28.629059145162365	21.03416618283006
40-41	20.90855160490494	28.805606087343328	28.98367811960488	21.302164188146854
42-43	20.882169561484805	28.63669342846229	28.993548993032636	21.487588017020265
44-45	21.124564203165182	28.825874388867994	28.834087189860035	21.215474218106785
46-47	21.082900779126152	29.136805314419405	28.72042699463532	21.05986691181912
48-49	20.96019977971213	28.891112006502112	28.804814294235147	21.34387391955061
50-51	21.049035603452726	28.709890670147963	28.724831996474254	21.516241729925056
52-53	21.061730478172084	28.735516751727214	28.806628115823298	21.396124654277404
54-55	21.21871834025274	29.241999586949557	28.503976830146826	21.035305242650875
56-57	21.093610404774445	28.891133536354445	28.52992779340877	21.485328265462336
58-59	20.851121351516717	28.464501545885668	29.086006533170988	21.59837056942663
60-61	21.22899713518302	28.9502419463356	28.541374388528045	21.279386529953335
62-63	20.976462932986838	29.016960990960794	28.600604917685214	21.40597115836716
64-65	20.988135897092945	28.612884197037697	28.806357334599575	21.592622571269786
66-67	20.86685599742897	28.75119957930111	28.63421455277468	21.747729870495238
68-69	21.405017711315118	28.8901979772552	28.524364695756887	21.180419615672797
70-71	21.3339544919975	28.6915544780133	28.57604254449029	21.398448485498914
72-73	21.031560277705566	28.807490903754495	28.769258318331296	21.391690500208643
74-75	21.12886761595378	28.90286156118934	28.573719623022413	21.394551199834464
76-77	21.130139680070993	28.92273602452343	28.46464929373384	21.482475001671737
78-79	21.334032223611285	28.990437546911384	28.54392958307835	21.131600646398986
80-81	21.23628756040363	28.747402925875953	28.61064689120345	21.405662622516967
82-83	21.05830637972335	28.832835858510926	28.801800421203932	21.30705734056179
84-85	21.056176277044873	28.775044110312976	28.627786184580877	21.540993428061274
86-87	21.287347529531832	28.892839992301923	28.469384582337458	21.35042789582879
88-89	21.06136391164351	28.807148379104063	28.55061524867232	21.580872460580107
90-91	21.01993383635156	28.66537325316527	28.69681104545596	21.617881865027215
92-93	21.197016198095312	28.9066681949039	28.464739380681863	21.431576226318928
94-95	21.00202835387124	28.62293651421057	28.671994186239097	21.703040945679096
96-97	20.96247014331008	28.678009888653573	28.7132292495878	21.646290718448544
98-99	21.03671560536181	28.699941348353793	28.587596822974863	21.675746223309538
100	21.17087546364514	28.72813373304202	28.468716020527413	21.632274782785426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	510.0
1	456.0
2	382.0
3	348.5
4	329.5
5	308.5
6	291.5
7	280.5
8	275.0
9	260.5
10	226.5
11	221.5
12	221.5
13	258.5
14	312.5
15	367.5
16	474.0
17	680.0
18	1010.0
19	1433.5
20	2088.0
21	3138.0
22	4774.0
23	7348.5
24	10541.5
25	14312.0
26	19479.5
27	26123.0
28	34277.5
29	42542.0
30	50477.0
31	58831.5
32	66577.0
33	73117.5
34	79333.5
35	84927.0
36	87868.0
37	90364.5
38	92851.0
39	93249.5
40	92982.0
41	92255.5
42	90041.5
43	87639.5
44	85382.0
45	82493.5
46	80130.0
47	77394.5
48	75016.5
49	73075.5
50	70492.0
51	67234.5
52	62053.0
53	57363.5
54	54341.5
55	51974.5
56	48264.0
57	43347.0
58	38896.5
59	34592.0
60	30329.5
61	25709.5
62	21057.5
63	16592.0
64	12998.5
65	10254.5
66	7906.5
67	5839.0
68	4140.0
69	3072.0
70	2218.5
71	1550.0
72	1154.0
73	826.0
74	629.0
75	480.5
76	299.0
77	208.5
78	154.0
79	103.0
80	66.0
81	41.5
82	32.5
83	27.0
84	23.5
85	16.5
86	12.5
87	10.0
88	8.0
89	6.0
90	3.5
91	1.5
92	2.0
93	4.0
94	2.0
95	0.0
96	0.5
97	2.0
98	2.0
99	1.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060860192599351826
2	0.04135892928313763
3	0.057284960991379225
4	0.07487672560788079
5	0.0883650994015956
6	0.08751191913151124
7	0.09031522573321703
8	0.10221912188248947
9	0.09624685999189884
10-11	0.10122374490072435
12-13	0.10516462519587597
14-15	0.1072975758710869
16-17	0.07518143284719664
18-19	0.09740474750129907
20-21	0.09049805007680653
22-23	0.05635052545747729
24-25	0.022426452813646334
26-27	0.18089453107384112
28-29	0.06614178474749317
30-31	0.07768003220958657
32-33	0.07260157822098913
34-35	0.02098417188088466
36-37	0.05303937345691175
38-39	0.07601429930132661
40-41	0.08048333881129237
42-43	0.07477515652810884
44-45	0.07329224796343839
46-47	0.07950827564548166
48-49	0.07597367166941783
50-51	0.07142337689563452
52-53	0.0754251986386493
54-55	0.06626366764321952
56-57	0.0690872880608797
58-59	0.06597927421985805
60-61	0.062404042611885445
62-63	0.06929042622042358
64-65	0.06524797684550002
66-67	0.06754343804834606
68-69	0.06711684791330388
70-71	0.059316342586818206
72-73	0.05832096560505311
74-75	0.060860192599351826
76-77	0.055436403739529745
78-79	0.052348703714462506
80-81	0.04706711156632116
82-83	0.052328389898508104
84-85	0.04347156614239416
86-87	0.04166363652245347
88-89	0.0435934490381205
90-91	0.03918535097601792
92-93	0.041765205602225415
94-95	0.041013594411913
96-97	0.044060666805071466
98-99	0.04428411878056976
100	0.050947050413609606
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2461379.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.77513623204024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7367415911228	57.948906266338675
2	12.633663062061498	18.135657747783174
3	3.4770517211363394	7.486975829112323
4	1.3472176705808634	3.8678692736061353
5	0.6300998294672397	2.261275054989823
6	0.3337306308470895	1.4372136896312748
7	0.19461687942068118	0.9778057123431759
8	0.1263631134039825	0.7255783743420476
9	0.09818312002642524	0.6342396134025087
>10	0.39531372590366254	4.809646304801691
>50	0.020135553142082943	0.9772986346801346
>100	0.006826503849225831	0.7086498088370258
>500	5.659903817184738E-5	0.028883690132000088
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.125526381755918E-5	0.0
5	0.0	0.0	0.0	8.125526381755918E-5	0.0
6	0.0	0.0	0.0	8.125526381755918E-5	0.0
7	0.0	0.0	0.0	8.125526381755918E-5	0.0
8	0.0	0.0	0.0	8.125526381755918E-5	0.0
9	0.0	0.0	0.0	2.4376579145267755E-4	0.0
10-11	0.0	0.0	0.0	3.0470723931584696E-4	0.0
12-13	0.0	0.0	0.0	4.265901350421857E-4	0.0
14-15	0.0	0.0	0.0	6.094144786316939E-4	0.0
16-17	0.0	0.0	0.0	6.500421105404735E-4	0.0
18-19	0.0	0.0	0.0	7.516111903124224E-4	0.0
20-21	0.0	0.0	0.0	8.734940860387612E-4	0.0
22-23	0.0	0.0	0.0	9.953769817650998E-4	0.0
24-25	0.0	0.0	0.0	0.0010766322455826591	0.0
26-27	0.0	0.0	0.0	0.0012594565891721672	0.0
28-29	0.0	0.0	0.0	0.0018485572518494714	0.0
30-31	0.0	0.0	0.0	0.004326842798285026	0.0
32-33	0.0	0.0	0.0	0.009527179682608813	0.0
34-35	0.0	0.0	0.0	0.017998040935589356	0.0
36-37	0.0	0.0	0.0	0.03331465816519927	0.0
38-39	0.0	0.0	0.0	0.062200904452341554	0.0
40-41	0.0	0.0	0.0	0.09659219486312348	0.0
42-43	0.0	0.0	0.0	0.13067877803458955	0.0
44-45	0.0	0.0	0.0	0.17055479875305674	0.0
46-47	0.0	0.0	0.0	0.2181906971661008	0.0
48-49	0.0	0.0	0.0	0.2734645903779954	0.0
50-51	0.0	0.0	0.0	0.3378593869534111	0.0
52-53	0.0	0.0	0.0	0.4007915887801107	0.0
54-55	0.0	0.0	0.0	0.469391345258085	0.0
56-57	0.0	0.0	0.0	0.5505044123639635	0.0
58-59	0.0	0.0	0.0	0.6478075907854906	0.0
60-61	0.0	0.0	0.0	0.7534191199323631	0.0
62-63	0.0	0.0	0.0	0.8600057122450464	0.0
64-65	0.0	0.0	0.0	0.9695987493189793	0.0
66-67	0.0	0.0	0.0	1.0994243470834846	0.0
68-69	0.0	0.0	0.0	1.2474308101271685	0.0
70-71	0.0	0.0	0.0	1.4122367989651328	0.0
72-73	0.0	0.0	0.0	1.591810932001939	0.0
74-75	0.0	0.0	0.0	1.7632392248410342	0.0
76-77	0.0	0.0	0.0	1.9430571236692926	0.0
78-79	0.0	0.0	0.0	2.1590742425282734	0.0
80-81	0.0	0.0	0.0	2.3987975846060277	0.0
82-83	0.0	0.0	0.0	2.6554017077418797	0.0
84-85	0.0	2.0313815954389796E-5	0.0	2.9035552834407055	0.0
86-87	0.0	4.062763190877959E-5	0.0	3.168000539534952	0.0
88	0.0	4.062763190877959E-5	0.0	3.3843629932651575	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3295	0.0	35.956085	1
TCAACGC	3125	0.0	35.80004	4
ATCAACG	3175	0.0	35.532364	3
GGTATCA	1985	0.0	35.29011	1
CAACGCA	3305	0.0	33.850956	5
AACGCAG	3555	0.0	31.462116	6
TATCAAC	4050	0.0	28.784142	2
GTACATG	6795	0.0	26.914568	1
ACGCAGA	4235	0.0	26.188946	7
CGCAGAG	4210	0.0	26.146729	8
TACATGG	6795	0.0	25.52663	2
ACATGGG	6825	0.0	24.38132	3
TATACCG	135	4.074591E-4	20.892141	5
CCGGTCG	160	5.3757933E-5	20.582447	9
GCAGAGT	5305	0.0	20.042044	9
CTGTCGC	635	0.0	20.00364	9
CATGGGG	4325	0.0	19.998081	4
TAGTCGC	145	6.607665E-4	19.44616	6
TTAGTCG	220	1.4873913E-6	19.230263	5
ATACCGC	175	1.07925356E-4	18.797955	6
>>END_MODULE