FastQCFastQC Report
Wed 25 May 2016
SRR1033036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461379
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50020.20321941480771552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42080.1709610750721445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36550.054.6903651
GTATCAA72650.044.192021
ATCAACG82950.038.302613
TCAACGC83300.038.082934
CAACGCA85550.037.1355175
AACGCAG88800.035.935166
TATCAAC89300.035.6717222
ACGCAGA101750.031.2692177
CGCAGAG102550.030.933638
GCAGAGT115550.027.1687269
GTACATG64150.026.5303461
TACATGG62900.025.7713182
TAGATCG950.001305623624.734845
ACATGGG66100.023.5355643
GAGTACT81850.022.50764812-13
CAGAGTA114350.021.82333610-11
AGAGTAC105600.021.51767310-11
GTGGTAT19600.020.6859171
GTACTTT91550.020.0202314-15
CATGGGG41900.019.965384
Produced by FastQC (version 0.11.5)