##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033036_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2461379 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68449474867544 34.0 31.0 34.0 31.0 34.0 2 32.686246205887024 34.0 31.0 34.0 31.0 34.0 3 32.79174804042774 34.0 31.0 34.0 31.0 34.0 4 36.287377522925155 37.0 37.0 37.0 35.0 37.0 5 36.27460947704518 37.0 37.0 37.0 35.0 37.0 6 36.34564526633241 37.0 37.0 37.0 35.0 37.0 7 36.295113836593224 37.0 37.0 37.0 35.0 37.0 8 36.30592850593103 37.0 37.0 37.0 35.0 37.0 9 38.10433785288653 39.0 39.0 39.0 37.0 39.0 10-11 38.070397732328104 39.0 39.0 39.0 35.0 39.0 12-13 38.04125390685465 39.0 39.0 39.0 35.0 39.0 14-15 39.526395569312974 41.0 39.0 41.0 37.0 41.0 16-17 39.52974613011649 41.0 39.0 41.0 37.0 41.0 18-19 39.55680392982958 41.0 39.5 41.0 37.0 41.0 20-21 39.55471912289818 41.0 40.0 41.0 37.0 41.0 22-23 39.47945541909637 41.0 39.0 41.0 36.5 41.0 24-25 39.42676097423436 41.0 39.0 41.0 36.0 41.0 26-27 39.313228885108714 41.0 39.0 41.0 36.0 41.0 28-29 39.25699902371801 41.0 39.0 41.0 36.0 41.0 30-31 39.24002459596836 41.0 39.0 41.0 36.0 41.0 32-33 39.18264009727881 41.0 39.0 41.0 35.0 41.0 34-35 39.0954655906303 41.0 39.0 41.0 35.0 41.0 36-37 38.997167847779636 41.0 39.0 41.0 35.0 41.0 38-39 38.90080499589864 41.0 39.0 41.0 35.0 41.0 40-41 38.7926601307641 41.0 39.0 41.0 35.0 41.0 42-43 38.66989906877404 41.0 39.0 41.0 35.0 41.0 44-45 38.492353067122124 41.0 38.5 41.0 35.0 41.0 46-47 38.27642796984942 41.0 38.0 41.0 34.0 41.0 48-49 38.05795917654291 40.0 38.0 41.0 34.0 41.0 50-51 37.92228644999409 40.0 38.0 41.0 33.5 41.0 52-53 37.79594203086969 40.0 38.0 41.0 33.0 41.0 54-55 37.658360211897474 40.0 38.0 41.0 33.0 41.0 56-57 37.523057806213515 40.0 37.0 41.0 33.0 41.0 58-59 37.35945622352348 40.0 37.0 41.0 33.0 41.0 60-61 37.177795658449995 40.0 36.5 41.0 33.0 41.0 62-63 36.96251593111016 39.5 36.0 41.0 32.0 41.0 64-65 36.70620635830565 39.0 35.5 41.0 32.0 41.0 66-67 36.44380914113593 39.0 35.0 41.0 31.5 41.0 68-69 36.146209909160675 38.5 35.0 41.0 31.0 41.0 70-71 35.82736648846033 37.5 35.0 40.0 31.0 41.0 72-73 35.48345399062883 37.0 35.0 39.5 31.0 41.0 74-75 35.14239009920861 36.5 35.0 39.0 30.5 41.0 76-77 34.78088278968822 36.0 35.0 39.0 30.0 41.0 78-79 34.41350255283725 36.0 35.0 38.5 30.0 40.0 80-81 34.06900826731682 35.0 34.0 37.0 30.0 39.0 82-83 33.75176455962288 35.0 34.0 37.0 30.0 39.0 84-85 33.50266151616634 35.0 34.0 36.5 30.0 38.5 86-87 33.2721801477952 35.0 34.0 36.0 29.5 37.0 88-89 33.043859356888966 35.0 34.0 36.0 29.0 37.0 90-91 32.84293032483011 35.0 34.0 35.5 29.0 36.5 92-93 32.655577422249884 35.0 34.0 35.0 29.0 36.0 94-95 32.48467038192818 35.0 34.0 35.0 29.0 36.0 96-97 32.33361461197158 35.0 34.0 35.0 29.0 36.0 98-99 32.20020565707272 35.0 34.0 35.0 29.0 36.0 100 32.0914003897815 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13366759465633749 1101 2 0.015936597800632057 1101 3 0.10527179028479594 1101 4 0.1334213804409501 1101 5 0.11448365265177785 1101 6 0.16279168028451352 1101 7 0.19979813605088026 1101 8 0.19817250081315763 1101 9 0.25268182965637465 1101 10-11 0.2364596920129074 1101 12-13 0.2735421730494565 1101 14-15 0.3402853441126936 1101 16-17 0.39630051701339397 1101 18-19 0.3443176176655669 1101 20-21 0.37971676777615926 1101 22-23 0.40984145141315054 1101 24-25 0.43220664806808173 1101 26-27 0.4171822045942619 1101 28-29 0.3906485065516776 1101 30-31 0.39982627519523106 1101 32-33 0.43144550617213184 1101 34-35 0.46465180417321506 1101 36-37 0.3956568441148889 1101 38-39 0.375977243704547 1101 40-41 0.4152399003820122 1101 42-43 0.40071896021156306 1101 44-45 0.36489276376925517 1101 46-47 0.30556590535306327 1101 48-49 0.34280004343851544 1101 50-51 0.37383251180261823 1101 52-53 0.4102740703252792 1101 54-55 0.4516789215956649 1101 56-57 0.40705799992404934 1101 58-59 0.35724366940978314 1101 60-61 0.42889522697343097 1101 62-63 0.41521804140695906 1101 64-65 0.385195170781337 1101 66-67 0.3971264693104857 1101 68-69 0.4126911196863716 1101 70-71 0.37475140910800064 1101 72-73 0.40001473364995377 1101 74-75 0.44420203703816696 1101 76-77 0.3531681300414675 1101 78-79 0.38739661916248735 1101 80-81 0.4260919575531972 1101 82-83 0.38583249517718343 1101 84-85 0.4504868920492058 1101 86-87 0.3818435738683732 1101 88-89 0.40354628837943096 1101 90-91 0.4119954605984617 1101 92-93 0.41120228675062265 1101 94-95 0.3538565460748444 1101 96-97 0.3806893062791943 1101 98-99 0.44020008471629524 1101 100 0.4411179977718547 1102 1 0.18104134133886873 1102 2 0.16659667275999368 1102 3 0.15867160552475212 1102 4 0.11514288179840548 1102 5 0.1520172175173471 1102 6 0.1219176077078572 1102 7 0.15996640444197396 1102 8 0.16361155861124388 1102 9 0.1754614575945297 1102 10-11 0.20643460522548196 1102 12-13 0.22042458881307425 1102 14-15 0.36192757553245514 1102 16-17 0.3278501157214109 1102 18-19 0.2946077080315064 1102 20-21 0.28992429581903423 1102 22-23 0.3325484313444633 1102 24-25 0.34657592216028377 1102 26-27 0.3802799899262794 1102 28-29 0.3497578977279332 1102 30-31 0.36933919539241344 1102 32-33 0.2855697948348066 1102 34-35 0.3122878454625493 1102 36-37 0.28080118139784105 1102 38-39 0.37201616222655787 1102 40-41 0.4199474354853052 1102 42-43 0.42147362250423726 1102 44-45 0.41499545572164465 1102 46-47 0.37011170852787245 1102 48-49 0.3807651300327066 1102 50-51 0.36812165384266393 1102 52-53 0.32240519124501077 1102 54-55 0.29456738776769953 1102 56-57 0.35402978574211375 1102 58-59 0.32603363974858723 1102 60-61 0.33462644867136504 1102 62-63 0.3459377620521451 1102 64-65 0.43657412656749983 1102 66-67 0.40624463027171487 1102 68-69 0.4085156243979 1102 70-71 0.39797433852832853 1102 72-73 0.3998709240405063 1102 74-75 0.37845828495255773 1102 76-77 0.33264116629710827 1102 78-79 0.34634269167374043 1102 80-81 0.37536999243761926 1102 82-83 0.3658216853024072 1102 84-85 0.36681441081691446 1102 86-87 0.4220972584786651 1102 88-89 0.4715361830602518 1102 90-91 0.3890027380335823 1102 92-93 0.5111889834327528 1102 94-95 0.4695288950171559 1102 96-97 0.4250034756504988 1102 98-99 0.44741741336547136 1102 100 0.410928570416516 1103 1 0.04339268660694984 1103 2 0.08091069716106603 1103 3 0.10294627267802525 1103 4 0.06793388792529953 1103 5 0.018925035785272826 1103 6 0.04376350736142598 1103 7 0.10081142436200707 1103 8 0.1248498527944264 1103 9 0.09515133554658917 1103 10-11 0.14069680061152923 1103 12-13 0.12475587416838607 1103 14-15 0.17711501415879383 1103 16-17 0.14521878229671614 1103 18-19 0.18959643977383678 1103 20-21 0.21384340932596047 1103 22-23 0.2073003628175485 1103 24-25 0.13527150595472648 1103 26-27 0.10725514792684976 1103 28-29 0.13213709767454418 1103 30-31 0.14209812923204623 1103 32-33 0.123553976671964 1103 34-35 0.20301636636705211 1103 36-37 0.1308068794298407 1103 38-39 0.07863869547573188 1103 40-41 0.10802074448336185 1103 42-43 0.08464697545348088 1103 44-45 0.08423028990358716 1103 46-47 0.02860184720483261 1103 48-49 0.11406128710952146 1103 50-51 0.032242992457710784 1103 52-53 0.01724282750962658 1103 54-55 0.11822492432919063 1103 56-57 0.07084611598229884 1103 58-59 0.08279388431308377 1103 60-61 0.12294358373637948 1103 62-63 0.050587906437790764 1103 64-65 0.05198776463844723 1103 66-67 0.07937977623009118 1103 68-69 0.061972856496311124 1103 70-71 -0.0028727156423826727 1103 72-73 -0.014598420547365265 1103 74-75 0.05555232058637927 1103 76-77 0.0050880219419866535 1103 78-79 -0.0028328865256241897 1103 80-81 0.036274339343741246 1103 82-83 0.062145780131864115 1103 84-85 0.05586268681609852 1103 86-87 0.03900738436079365 1103 88-89 0.014665048757819932 1103 90-91 0.019338119581618685 1103 92-93 0.11870055168026283 1103 94-95 0.09361268342384932 1103 96-97 0.019930913609400136 1103 98-99 0.09739538452525665 1103 100 0.06857429980366447 1104 1 0.12083416589328522 1104 2 0.11410066777433059 1104 3 0.12889099695894402 1104 4 0.09255088616637863 1104 5 0.10230462415672292 1104 6 0.09987884745145692 1104 7 0.11895701357308042 1104 8 0.11942545919295355 1104 9 0.18421912188647838 1104 10-11 0.17806914172798827 1104 12-13 0.2023710190833512 1104 14-15 0.2199334256949399 1104 16-17 0.23373443747896516 1104 18-19 0.22565837956618395 1104 20-21 0.22471387334712745 1104 22-23 0.21137937088634828 1104 24-25 0.2366447784211161 1104 26-27 0.2493677823482301 1104 28-29 0.293602611279546 1104 30-31 0.23135832871903972 1104 32-33 0.22665729357667175 1104 34-35 0.23813741507645148 1104 36-37 0.23846902370404877 1104 38-39 0.250949648512524 1104 40-41 0.30069970461207873 1104 42-43 0.2641355498453066 1104 44-45 0.27248960624854845 1104 46-47 0.20033790424014342 1104 48-49 0.13544057217290373 1104 50-51 0.2577251719370821 1104 52-53 0.2558849280083777 1104 54-55 0.17079639756944687 1104 56-57 0.2147251181590235 1104 58-59 0.15191581750362815 1104 60-61 0.1782161224382861 1104 62-63 0.1899260755149612 1104 64-65 0.21094922911424163 1104 66-67 0.22832378877485127 1104 68-69 0.29050276314036694 1104 70-71 0.30458717303753247 1104 72-73 0.3231700958075052 1104 74-75 0.2621947505058131 1104 76-77 0.19470803987265128 1104 78-79 0.16625751066064254 1104 80-81 0.20710457319895426 1104 82-83 0.19529592900723713 1104 84-85 0.22776688565762981 1104 86-87 0.215854902088914 1104 88-89 0.24551747164024107 1104 90-91 0.1905480743800112 1104 92-93 0.14576150655832976 1104 94-95 0.15077897257352646 1104 96-97 0.22516744703253977 1104 98-99 0.25639919830616265 1104 100 0.25748417177819505 1105 1 -5.74171457714101E-4 1105 2 0.07123061555747512 1105 3 0.048142997499731166 1105 4 0.03221261174601153 1105 5 0.025214929524572938 1105 6 0.022908088381107916 1105 7 0.03213819866869727 1105 8 0.06340840389716362 1105 9 0.12043554251914657 1105 10-11 0.03503972223556673 1105 12-13 0.03693611669341834 1105 14-15 0.0408859815659568 1105 16-17 0.06165843478522248 1105 18-19 0.060963556101000904 1105 20-21 0.036519391702107384 1105 22-23 0.0658057507301848 1105 24-25 0.03670580862628725 1105 26-27 0.05744675938760935 1105 28-29 0.08045972152164893 1105 30-31 0.07830468208455699 1105 32-33 0.05251857198409482 1105 34-35 0.027869385975819227 1105 36-37 0.04979401688040497 1105 38-39 0.07885626783454569 1105 40-41 0.018963041338103892 1105 42-43 0.023100517104545304 1105 44-45 0.017355607641334814 1105 46-47 0.020422358054879908 1105 48-49 0.042145986961472204 1105 50-51 0.05498324488762307 1105 52-53 0.033581608526411344 1105 54-55 0.012902743180248422 1105 56-57 0.005748958840214868 1105 58-59 0.03314797716935658 1105 60-61 -0.001825214843833578 1105 62-63 0.02113148265084419 1105 64-65 -0.007838476328608124 1105 66-67 0.029601437307199774 1105 68-69 0.011769143086240774 1105 70-71 0.021395223157547605 1105 72-73 0.05871065634156025 1105 74-75 -0.010815294925663466 1105 76-77 -0.031224886847738276 1105 78-79 -0.0013558575689529562 1105 80-81 0.024609751519392375 1105 82-83 0.057434148073156166 1105 84-85 0.044928649500711515 1105 86-87 0.013364716664227672 1105 88-89 -0.01440360361377202 1105 90-91 0.0041929487355929496 1105 92-93 0.04787179968188582 1105 94-95 0.04348187727966746 1105 96-97 2.486612083814066E-4 1105 98-99 -0.10143693336092952 1105 100 -0.05805888863469022 1106 1 0.07647887366904627 1106 2 0.08092573713700091 1106 3 0.10385379122600114 1106 4 0.0757185154698945 1106 5 0.04193843896382532 1106 6 0.054799169704367046 1106 7 0.08620664772964659 1106 8 0.08830127127215093 1106 9 0.07488832796740041 1106 10-11 0.10694933460747791 1106 12-13 0.15588350436417642 1106 14-15 0.1980736206250242 1106 16-17 0.20772188229175725 1106 18-19 0.1779476584118953 1106 20-21 0.1619100524193371 1106 22-23 0.17884392834783824 1106 24-25 0.145468129640129 1106 26-27 0.14281965102365035 1106 28-29 0.1491674704259438 1106 30-31 0.12324879939097144 1106 32-33 0.16221727481069337 1106 34-35 0.10656668068654085 1106 36-37 0.08823382754672338 1106 38-39 0.11101632367152092 1106 40-41 0.12883951117333936 1106 42-43 0.09631423678585804 1106 44-45 0.031941493565661006 1106 46-47 0.030474004209388283 1106 48-49 0.09343996010364464 1106 50-51 0.09509161189991744 1106 52-53 0.1639231528211056 1106 54-55 0.20617190361402038 1106 56-57 0.13050330053080472 1106 58-59 0.15315145174098177 1106 60-61 0.21229968076662686 1106 62-63 0.20381158127990773 1106 64-65 0.14435865684502147 1106 66-67 0.18141225297812724 1106 68-69 0.21662180905799744 1106 70-71 0.17129860568350352 1106 72-73 0.15083267476288853 1106 74-75 0.16384166732342464 1106 76-77 0.13148213971140166 1106 78-79 0.21882027882931254 1106 80-81 0.20263895316036695 1106 82-83 0.26103970190158776 1106 84-85 0.16896986584460905 1106 86-87 0.17222213121719676 1106 88-89 0.2050085842081586 1106 90-91 0.22575098932102833 1106 92-93 0.2101400853770059 1106 94-95 0.2763459910505546 1106 96-97 0.22216155004038285 1106 98-99 0.2029660956121262 1106 100 0.1992140907799964 1107 1 0.05180059674859194 1107 2 0.02882320213140588 1107 3 0.05276713510077968 1107 4 0.04378108094159927 1107 5 0.03774194579121115 1107 6 0.0810357189123323 1107 7 0.03971065282004105 1107 8 -8.812827051158934E-4 1107 9 0.061049635576083006 1107 10-11 0.03997983956335105 1107 12-13 0.05544711188510831 1107 14-15 0.10634682445542154 1107 16-17 0.08508573597529079 1107 18-19 0.10891821932980861 1107 20-21 0.07782302023272791 1107 22-23 0.16649855885103904 1107 24-25 0.1520505966761121 1107 26-27 0.09631621552330216 1107 28-29 0.14428435367611314 1107 30-31 0.0972493594512045 1107 32-33 0.10212614773346473 1107 34-35 0.1253885162912738 1107 36-37 0.1265659183380592 1107 38-39 0.16576536317555934 1107 40-41 0.2100095752210649 1107 42-43 0.25764222462287734 1107 44-45 0.1822184814679204 1107 46-47 0.21441867553686933 1107 48-49 0.06632498135148523 1107 50-51 0.1416247731100313 1107 52-53 0.16232258279121936 1107 54-55 0.1536605393029049 1107 56-57 0.15923904520198562 1107 58-59 0.12949425844909968 1107 60-61 0.16450719041190354 1107 62-63 0.10564962710955683 1107 64-65 0.09489093398099868 1107 66-67 0.12180443442004929 1107 68-69 0.1465455558315938 1107 70-71 0.17022979964540497 1107 72-73 0.15788518187542877 1107 74-75 0.11058276188930449 1107 76-77 0.16690591250874576 1107 78-79 0.202927067067165 1107 80-81 0.19693979053837296 1107 82-83 0.17583570142949156 1107 84-85 0.22931038099738998 1107 86-87 0.22933460289347352 1107 88-89 0.13588267401866005 1107 90-91 0.17063820389373063 1107 92-93 0.19759924459758338 1107 94-95 0.15120771701835878 1107 96-97 0.15753422833782338 1107 98-99 0.26733860457198944 1107 100 0.2980852468718638 1108 1 -0.007710911331699322 1108 2 -0.018264806549261436 1108 3 0.01343638475106701 1108 4 0.0021255175258971803 1108 5 0.046558725854815464 1108 6 0.007737008550940061 1108 7 0.008965990528210455 1108 8 0.04070270867556758 1108 9 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-0.0013980590712066032 2214 50-51 -0.0017231192889823888 2214 52-53 -0.09271924677770471 2214 54-55 -0.08088167332871876 2214 56-57 -0.019242624009891074 2214 58-59 -0.011746362246633169 2214 60-61 0.0035442676130017503 2214 62-63 -0.03709833495053516 2214 64-65 -0.02917759924130081 2214 66-67 0.013033469328874503 2214 68-69 -0.02049161210450734 2214 70-71 0.050774879210493395 2214 72-73 0.0433280613849476 2214 74-75 -0.01499268585257596 2214 76-77 0.03926874349943432 2214 78-79 0.02965677626879426 2214 80-81 0.09145827425887632 2214 82-83 0.018430926231566502 2214 84-85 0.030037773285549463 2214 86-87 -0.0018895928151039243 2214 88-89 0.035968982033267594 2214 90-91 -1.6495456251419682E-4 2214 92-93 -0.025777501289027782 2214 94-95 -0.020304400129305122 2214 96-97 -0.004293177097153489 2214 98-99 -0.03541288566509593 2214 100 -0.0343846404484367 2215 1 -0.15846272351968338 2215 2 -0.10087930467745565 2215 3 -0.14945241779337692 2215 4 -0.0833121967044832 2215 5 -0.17166449975350417 2215 6 -0.1639270050559105 2215 7 -0.2063267068458572 2215 8 -0.19831621813361267 2215 9 -0.2089462943890581 2215 10-11 -0.20495469615638484 2215 12-13 -0.22251654622787242 2215 14-15 -0.29326206434701163 2215 16-17 -0.34461763539326995 2215 18-19 -0.28994204482800967 2215 20-21 -0.27645745386059417 2215 22-23 -0.34991321580363177 2215 24-25 -0.34624030688903673 2215 26-27 -0.39798384444952006 2215 28-29 -0.39743635547657874 2215 30-31 -0.41996426430453937 2215 32-33 -0.31907214761897507 2215 34-35 -0.30483380103251534 2215 36-37 -0.37890359251387196 2215 38-39 -0.39399885698528436 2215 40-41 -0.3326380139752345 2215 42-43 -0.2999991712639911 2215 44-45 -0.337678099180458 2215 46-47 -0.384221041843368 2215 48-49 -0.2939415179748579 2215 50-51 -0.28643839642465707 2215 52-53 -0.25596031519127393 2215 54-55 -0.30998769729249886 2215 56-57 -0.2563379310208802 2215 58-59 -0.22273967075199153 2215 60-61 -0.1836170782771589 2215 62-63 -0.1849738567214132 2215 64-65 -0.2585377686925412 2215 66-67 -0.26167840470034065 2215 68-69 -0.2776350502091418 2215 70-71 -0.30469175507068513 2215 72-73 -0.28701036460345364 2215 74-75 -0.27723236186551503 2215 76-77 -0.23906583548146898 2215 78-79 -0.27799074950893043 2215 80-81 -0.3162448049050255 2215 82-83 -0.2952848826142045 2215 84-85 -0.24522245206807725 2215 86-87 -0.2009739002252715 2215 88-89 -0.2499794153807784 2215 90-91 -0.1976218102742422 2215 92-93 -0.15399644141762536 2215 94-95 -0.19750910527805132 2215 96-97 -0.23829665465920868 2215 98-99 -0.29037414750945345 2215 100 -0.22689323369688807 2216 1 -0.17558075127084294 2216 2 -0.19498157401634586 2216 3 -0.18877076645706126 2216 4 -0.16408129043024644 2216 5 -0.23133361141994158 2216 6 -0.23488465184352947 2216 7 -0.22190133660849654 2216 8 -0.21910816493997487 2216 9 -0.2883940967190952 2216 10-11 -0.2813784299504505 2216 12-13 -0.29084125135240413 2216 14-15 -0.39206879559528574 2216 16-17 -0.358263702887605 2216 18-19 -0.3278587513454738 2216 20-21 -0.3025693800998468 2216 22-23 -0.36341353636513674 2216 24-25 -0.34450795721046035 2216 26-27 -0.3499919905057354 2216 28-29 -0.3464437063440755 2216 30-31 -0.35113016619651916 2216 32-33 -0.35490264379183856 2216 34-35 -0.4844839420358227 2216 36-37 -0.4197456962686701 2216 38-39 -0.33765295330357503 2216 40-41 -0.2698591214970847 2216 42-43 -0.27257359921209456 2216 44-45 -0.29428241876178873 2216 46-47 -0.20653519058149783 2216 48-49 -0.2967481922899964 2216 50-51 -0.39759699991034836 2216 52-53 -0.3678853282741201 2216 54-55 -0.35007002723922653 2216 56-57 -0.4332794919825531 2216 58-59 -0.3991974442206754 2216 60-61 -0.45634514878540244 2216 62-63 -0.48494915042840603 2216 64-65 -0.5972311125777381 2216 66-67 -0.5561945357025522 2216 68-69 -0.49004771685339676 2216 70-71 -0.5073108385133764 2216 72-73 -0.4510164404858159 2216 74-75 -0.4965324005598575 2216 76-77 -0.4268750157686938 2216 78-79 -0.4652990238907293 2216 80-81 -0.4413389020773124 2216 82-83 -0.2916835965982827 2216 84-85 -0.44907874953531035 2216 86-87 -0.48849968423294143 2216 88-89 -0.5634369495282812 2216 90-91 -0.5552135448892201 2216 92-93 -0.47604039813477783 2216 94-95 -0.38725564746693664 2216 96-97 -0.5774890291181336 2216 98-99 -0.5894749596194409 2216 100 -0.6174501980753462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 0.0 4 6.0 5 19.0 6 70.0 7 156.0 8 324.0 9 656.0 10 1213.0 11 2000.0 12 3083.0 13 4925.0 14 5816.0 15 7500.0 16 9367.0 17 12991.0 18 15747.0 19 8141.0 20 5397.0 21 4799.0 22 4815.0 23 5055.0 24 5831.0 25 7064.0 26 9108.0 27 11753.0 28 15182.0 29 19566.0 30 25379.0 31 32184.0 32 40701.0 33 53204.0 34 73718.0 35 109229.0 36 190997.0 37 436976.0 38 863818.0 39 472439.0 40 2139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41898672693167 20.48596786092442 13.747086164182837 23.34795924796108 2 15.347522109045771 20.6819148087032 40.75736818871164 23.213194893539384 3 17.59156181641482 26.23081898583181 30.994259915946447 25.183359281806922 4 11.683610888668682 17.32361331014244 40.32281983056997 30.66995597061891 5 11.65181589484906 38.35078427676227 36.723361241841964 13.274038586546713 6 29.42351328017876 39.695292265502005 17.899385506068764 12.98180894825047 7 25.718387944319016 33.41179883309315 23.524577076508738 17.34523614607909 8 24.379492413174443 36.63225225605882 20.939581664210042 18.0486736665567 9 24.652603276456002 16.185479765611067 22.190731293311593 36.97118566462134 10-11 23.04393638409249 27.00870715868895 29.8785775619265 20.068778895292063 12-13 24.172039332423005 24.609497359000787 30.03399720238127 21.18446610619494 14-15 21.308874902569645 26.49817266660261 27.635301997035395 24.557650433792354 16-17 20.00802397034178 30.010583515311563 28.10721649484536 21.874176019501295 18-19 19.752458443274044 28.414604988411917 31.219085998843326 20.613850569470713 20-21 21.159382841769357 27.688041477570398 30.834875660122975 20.31770002053727 22-23 20.6535000530191 27.528879159917395 31.013635863177402 20.8039849238861 24-25 20.519442898882463 27.674098162044096 30.908869670426597 20.89758926864684 26-27 20.299230938460656 27.604310027336332 31.0872188793547 21.009240154848314 28-29 20.361651222059848 27.669755588030604 31.126763596149637 20.84182959375992 30-31 20.689516477971477 28.234012912284467 30.60057439392665 20.475896215817407 32-33 20.24243320512607 27.816906701487255 31.14969291604422 20.790967177342456 34-35 20.395822829397666 27.627358484816845 31.11060913414797 20.866209551637517 36-37 20.861801453575417 28.061606928473836 30.379067993998486 20.697523623952264 38-39 20.65137063410389 28.206911653995586 30.518502026709417 20.623215685191106 40-41 20.49228947861299 27.954311753136558 30.64457415503598 20.90882461321447 42-43 20.51642595471888 27.904012344299677 30.45276651828101 21.126795182700427 44-45 20.80541030048603 28.062277284400327 30.225779938806664 20.906532476306978 46-47 20.84234081789111 28.43704687494287 29.849385242987776 20.871227064178253 48-49 20.82669917960623 28.36509533883242 29.58337582306504 21.224829658496315 50-51 20.90541521642949 28.284876079628535 29.361833346266465 21.447875357675514 52-53 20.918761393511524 28.358940252598234 29.390577395841927 21.331720958048315 54-55 21.0357283457769 28.769279334876913 29.1660284742821 21.028963845064087 56-57 20.878966628056876 28.41949573795827 29.205091942362394 21.49644569162246 58-59 20.67863177511468 28.014174168220336 29.73193075913949 21.575263297525492 60-61 21.032478135224196 28.463820484370757 29.236476788011924 21.267224592393124 62-63 20.81989405126151 28.404301003624393 29.317224206430627 21.458580738683477 64-65 20.80935118078118 28.137255579087984 29.444916853519914 21.60847638661092 66-67 20.703414630579037 28.233807146319194 29.278790466644917 21.78398775645685 68-69 21.249411913833494 28.354142848669078 29.180449618790156 21.215995618707275 70-71 21.205779606834138 28.144574270068585 29.142510995362965 21.507135127734315 72-73 20.97270605949003 28.30782687937472 29.2837059678744 21.435761093260847 74-75 21.04672024649647 28.356539883820986 29.088143451584358 21.508596418098183 76-77 21.031543699690296 28.355385334806222 28.96723747135244 21.64583349415104 78-79 21.160800510608077 28.471783500224873 29.046034763439522 21.321381225727528 80-81 21.050516803791698 28.284327606597763 29.104558867204116 21.560596722406423 82-83 20.86936331547882 28.324725681895007 29.291522302971995 21.514388699654177 84-85 20.823489596685434 28.241242002958504 29.159812446600057 21.775455953756005 86-87 21.00529157494082 28.328746787098463 29.001442491982598 21.66451914597812 88-89 20.754695516880133 28.253993147146613 29.095739850782742 21.895571485190512 90-91 20.63096352965793 28.1538324025978 29.194434682273503 22.020769385470775 92-93 20.781851038692366 28.366710490606025 28.956708556718958 21.89472991398265 94-95 20.466107315677952 28.06756171221288 29.238335077961835 22.22799589414733 96-97 20.39729451814089 28.061004975678213 29.225355116839257 22.31634538934164 98-99 20.42769607544404 28.076493048191782 29.136002899193702 22.359807977170476 100 20.56066944586754 28.056264394877832 28.973473812850436 22.409592346404192 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61.0 1 48.0 2 112.5 3 183.5 4 125.5 5 103.0 6 233.0 7 525.0 8 915.0 9 1116.0 10 943.0 11 764.0 12 883.0 13 1078.5 14 1257.5 15 1531.5 16 1830.0 17 2168.5 18 2533.5 19 2993.5 20 3699.5 21 4625.0 22 6156.5 23 8678.0 24 11701.5 25 15297.0 26 20197.0 27 26585.5 28 34515.0 29 42435.0 30 49844.5 31 58015.5 32 66216.0 33 73017.5 34 78925.0 35 84270.0 36 87842.5 37 90464.0 38 92495.5 39 92553.0 40 92275.0 41 91804.0 42 89587.5 43 87299.5 44 85030.0 45 82363.0 46 79936.5 47 77385.5 48 75111.5 49 72604.0 50 69729.0 51 66201.0 52 60919.0 53 56371.5 54 53113.0 55 50716.0 56 47252.5 57 42444.0 58 38397.5 59 34297.5 60 30032.0 61 25370.5 62 20723.0 63 16378.0 64 12788.0 65 9997.5 66 7532.5 67 5404.0 68 3910.0 69 2941.0 70 1991.0 71 1368.0 72 1033.0 73 713.0 74 509.5 75 355.5 76 221.5 77 152.0 78 95.0 79 52.0 80 31.5 81 10.5 82 6.5 83 4.5 84 5.0 85 3.0 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04607173458455605 2 0.23657470060482358 3 0.0036971145036989424 4 0.002762678969797012 5 1.2188289572633876E-4 6 1.6251052763511836E-4 7 0.0 8 2.8439342336145714E-4 9 0.0 10-11 5.484730307685245E-4 12-13 0.0 14-15 2.2345197549828772E-4 16-17 1.6251052763511836E-4 18-19 5.89100662677304E-4 20-21 2.0313815954389796E-5 22-23 8.125526381755918E-5 24-25 0.0012391427732177775 26-27 1.0156907977194898E-4 28-29 8.125526381755918E-5 30-31 8.125526381755918E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.125526381755918E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.0313815954389796E-4 70-71 2.0313815954389796E-5 72-73 3.453348712246265E-4 74-75 2.0313815954389796E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.2188289572633876E-4 84-85 0.0 86-87 5.484730307685245E-4 88-89 0.002782992785751402 90-91 0.002600168442161894 92-93 6.094144786316939E-4 94-95 5.89100662677304E-4 96-97 2.4376579145267752E-4 98-99 2.0313815954389796E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2461379.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.77342915970823 #Duplication Level Percentage of deduplicated Percentage of total 1 79.7133503897512 55.61873806502307 2 13.25305009267431 18.494215035825512 3 3.6902619085819017 7.724466835776271 4 1.4133346044608575 3.944528076132556 5 0.6621575501979894 2.310050146065268 6 0.3454337523201109 1.4461258468127676 7 0.21600590807621944 1.0550031047664181 8 0.14350934048779426 0.8010511041825248 9 0.09424074925817329 0.5917950218090682 >10 0.43625743886037543 5.237462351763301 >50 0.022329850675738006 1.0663062724924903 >100 0.009601816850045778 1.102068955970352 >500 2.3329890269233974E-4 0.09530806741346447 >1k 1.7497417701657598E-4 0.30932391989720537 >5k 5.8324725672191993E-5 0.20355719606970418 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5002 0.20321941480771552 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4208 0.1709610750721445 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.062763190877959E-5 0.0 4 0.0 0.0 0.0 8.125526381755918E-5 0.0 5 0.0 0.0 0.0 8.125526381755918E-5 0.0 6 0.0 0.0 0.0 8.125526381755918E-5 0.0 7 0.0 0.0 0.0 1.2188289572633877E-4 0.0 8 0.0 0.0 0.0 1.2188289572633877E-4 0.0 9 0.0 0.0 0.0 2.8439342336145714E-4 0.0 10-11 0.0 0.0 0.0 3.453348712246265E-4 0.0 12-13 0.0 0.0 0.0 3.8596250313340614E-4 0.0 14-15 0.0 0.0 0.0 4.6721776695096527E-4 0.0 16-17 0.0 0.0 0.0 5.687868467229143E-4 0.0 18-19 0.0 0.0 0.0 6.094144786316938E-4 0.0 20-21 0.0 0.0 0.0 6.094144786316938E-4 0.0 22-23 0.0 0.0 0.0 6.90669742449253E-4 0.0 24-25 0.0 0.0 0.0 8.328664541299816E-4 0.0 26-27 0.0 0.0 0.0 9.547493498563203E-4 0.0 28-29 0.0 0.0 0.0 0.0015844776444424039 0.0 30-31 0.0 0.0 0.0 0.004062763190877959 0.0 32-33 0.0 0.0 0.0 0.009242786259247357 0.0 34-35 0.0 0.0 0.0 0.017612078432455953 0.0 36-37 0.0 0.0 0.0 0.033111520005655364 0.0 38-39 0.0 0.0 0.0 0.062363414979976675 0.0 40-41 0.0 0.0 0.0 0.09720160934175517 0.0 42-43 0.0 0.0 0.0 0.1313897615929932 0.0 44-45 0.0 0.0 0.0 0.17130640994336915 0.0 46-47 0.0 0.0 0.0 0.21900324980427638 0.0 48-49 0.0 0.0 0.0 0.27435839827998854 0.0 50-51 0.0 0.0 0.0 0.3387531948554042 0.0 52-53 0.0 0.0 0.0 0.4018682210256933 0.0 54-55 0.0 0.0 0.0 0.470955509086573 0.0 56-57 0.0 0.0 0.0 0.5521904590881779 0.0 58-59 0.0 0.0 0.0 0.6501843072521541 0.0 60-61 0.0 0.0 0.0 0.7561005436383426 0.0 62-63 0.0 0.0 0.0 0.8635606300370646 0.0 64-65 0.0 0.0 0.0 0.9738646506694011 0.0 66-67 0.0 0.0 0.0 1.104299662912538 0.0 68-69 0.0 0.0 0.0 1.2533015029379873 0.0 70-71 0.0 0.0 0.0 1.4186153371748111 0.0 72-73 0.0 0.0 0.0 1.5982504116594805 0.0 74-75 0.0 0.0 0.0 1.770572512400569 0.0 76-77 0.0 0.0 0.0 1.9515279849222733 0.0 78-79 0.0 0.0 0.0 2.1690889537937883 0.0 80-81 0.0 0.0 0.0 2.4099295557490334 0.0 82-83 0.0 0.0 0.0 2.6682197256090996 0.0 84-85 0.0 0.0 0.0 2.9191562940936766 0.0 86-87 0.0 0.0 0.0 3.1849828896728214 0.0 88 0.0 0.0 0.0 3.4024422894645645 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3655 0.0 54.690365 1 GTATCAA 7265 0.0 44.19202 1 ATCAACG 8295 0.0 38.30261 3 TCAACGC 8330 0.0 38.08293 4 CAACGCA 8555 0.0 37.135517 5 AACGCAG 8880 0.0 35.93516 6 TATCAAC 8930 0.0 35.671722 2 ACGCAGA 10175 0.0 31.269217 7 CGCAGAG 10255 0.0 30.93363 8 GCAGAGT 11555 0.0 27.168726 9 GTACATG 6415 0.0 26.530346 1 TACATGG 6290 0.0 25.771318 2 TAGATCG 95 0.0013056236 24.73484 5 ACATGGG 6610 0.0 23.535564 3 GAGTACT 8185 0.0 22.507648 12-13 CAGAGTA 11435 0.0 21.823336 10-11 AGAGTAC 10560 0.0 21.517673 10-11 GTGGTAT 1960 0.0 20.685917 1 GTACTTT 9155 0.0 20.02023 14-15 CATGGGG 4190 0.0 19.96538 4 >>END_MODULE