FastQCFastQC Report
Wed 25 May 2016
SRR1033035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238281
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42151.768919888702834No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2880.12086570058040717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAC1700.041.477911
GTTGCCG250.001696923437.6184924-25
TAGTATG500.001699598637.598747
TTAGTCT651.408663E-436.160234
GAGAACA3200.035.256221
CTAGCAT401.1608283E-535.2340284-85
TAGTCTA550.002710356434.187855
TATGGTC600.00415083131.3388655
GTATAAG902.582244E-531.3388651
GGGTAGG1052.0534553E-631.3388631
GTACATA753.264426E-431.3388611
ATAATCG452.5950741E-531.32570566-67
TAATCGG452.5950741E-531.32570568-69
TGCCGTG804.7705736E-429.3740167
GGGACGC650.006132908628.934269
TGTACAG650.00613920828.9281835
AGTCTAT650.006145512728.9221086
CATTACG505.325911E-528.19313448-49
TAGGAGT856.7930983E-427.651944
CCGTATC856.809805E-427.64032794
Produced by FastQC (version 0.11.5)