##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033035_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 238281 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.78252567346956 33.0 31.0 34.0 16.0 34.0 2 29.771257464925863 33.0 31.0 34.0 18.0 34.0 3 29.972024626386492 34.0 31.0 34.0 22.0 34.0 4 32.95883431746552 37.0 35.0 37.0 19.0 37.0 5 33.45443824727947 37.0 35.0 37.0 27.0 37.0 6 33.23167184962293 37.0 35.0 37.0 23.0 37.0 7 33.41162325153915 37.0 35.0 37.0 27.0 37.0 8 33.10758726041942 37.0 35.0 37.0 19.0 37.0 9 34.39096277084619 39.0 37.0 39.0 17.0 39.0 10-11 34.650618387534045 39.0 37.0 39.0 20.0 39.0 12-13 34.62597101741221 39.0 37.0 39.0 21.0 39.0 14-15 35.885758830960086 40.0 38.0 41.0 20.0 41.0 16-17 35.990723137807876 40.0 38.0 41.0 25.0 41.0 18-19 35.99217100817942 40.0 37.0 41.0 26.0 41.0 20-21 36.02244618748452 40.0 37.0 41.0 27.0 41.0 22-23 35.98718739639334 40.0 36.5 41.0 27.0 41.0 24-25 35.946407812624585 40.0 36.0 41.0 27.0 41.0 26-27 35.671996508324206 40.0 36.0 41.0 26.0 41.0 28-29 35.47321229976373 40.0 36.0 41.0 25.0 41.0 30-31 35.377957117856646 40.0 36.0 41.0 24.5 41.0 32-33 35.17793697357322 40.0 36.0 41.0 19.5 41.0 34-35 34.926441890037395 40.0 36.0 41.0 5.5 41.0 36-37 34.69348794070866 40.0 35.5 41.0 2.0 41.0 38-39 34.46745229372044 40.0 35.0 41.0 2.0 41.0 40-41 34.306904453145656 40.0 35.0 41.0 2.0 41.0 42-43 34.129001053378154 40.0 35.0 41.0 2.0 41.0 44-45 33.98215971898725 40.0 35.0 41.0 2.0 41.0 46-47 33.81532308492913 40.0 35.0 41.0 2.0 41.0 48-49 33.66796135654962 40.0 35.0 41.0 2.0 41.0 50-51 33.5247124193704 40.0 35.0 41.0 2.0 41.0 52-53 33.37796760967093 40.0 34.5 41.0 2.0 41.0 54-55 33.23789139713196 39.5 34.0 41.0 2.0 41.0 56-57 33.0721039445025 39.0 34.0 41.0 2.0 41.0 58-59 32.88098715382259 39.0 34.0 41.0 2.0 41.0 60-61 32.66586299369232 38.5 34.0 41.0 2.0 41.0 62-63 32.42003558823406 38.0 33.0 41.0 2.0 41.0 64-65 32.14017903231898 37.5 33.0 40.0 2.0 41.0 66-67 31.864084841007045 37.0 33.0 40.0 2.0 41.0 68-69 31.550020773792287 36.5 33.0 39.5 2.0 41.0 70-71 31.215661341021736 36.0 32.0 39.0 2.0 41.0 72-73 30.87610636181651 35.0 32.0 39.0 2.0 41.0 74-75 30.525094321410435 35.0 32.0 38.5 2.0 40.0 76-77 30.172011196864204 35.0 31.0 37.0 2.0 39.0 78-79 29.81856925226938 35.0 31.0 37.0 2.0 39.0 80-81 29.483393556347337 35.0 31.0 36.0 2.0 39.0 82-83 29.265646442645448 35.0 31.0 36.0 2.0 37.0 84-85 29.065903282259182 35.0 31.0 35.5 2.0 37.0 86-87 28.901683306684127 35.0 31.0 35.0 2.0 37.0 88-89 28.705568215678127 35.0 31.0 35.0 2.0 36.0 90-91 28.50616079334903 35.0 30.0 35.0 2.0 36.0 92-93 28.287758990435663 35.0 30.0 35.0 2.0 36.0 94-95 28.08527956488348 35.0 29.5 35.0 2.0 35.5 96-97 27.923013165128566 35.0 29.0 35.0 2.0 35.0 98-99 27.749726163647125 35.0 29.0 35.0 2.0 35.0 100 27.593119048518346 35.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.089201873039812 1101 2 1.272900985592326 1101 3 1.0776317994693265 1101 4 1.3249951243807203 1101 5 1.1893983725682489 1101 6 1.2580217036222763 1101 7 1.3305105803057344 1101 8 1.2682045473439203 1101 9 1.7248660666036528 1101 10-11 1.454202099978211 1101 12-13 1.5224912915855882 1101 14-15 1.6618564830686893 1101 16-17 1.6201003755374472 1101 18-19 1.8007340522698954 1101 20-21 1.8239540781942836 1101 22-23 1.7341747180309213 1101 24-25 1.6571182236523612 1101 26-27 1.5934832169972921 1101 28-29 1.4758662118422023 1101 30-31 1.4396601796835142 1101 32-33 1.3387643514363603 1101 34-35 1.3235615504073124 1101 36-37 1.1376433245101438 1101 38-39 1.1579762709308596 1101 40-41 1.2271123975210045 1101 42-43 1.253798056971192 1101 44-45 1.2886423143842265 1101 46-47 1.2424404815825767 1101 48-49 1.0844816728897868 1101 50-51 1.1083624620365526 1101 52-53 1.1981250969300845 1101 54-55 1.3027933211994451 1101 56-57 1.4159327342171935 1101 58-59 1.438876791927072 1101 60-61 1.2793427739056824 1101 62-63 1.2156493013364695 1101 64-65 1.2697135803007455 1101 66-67 1.1552263119361328 1101 68-69 1.2766887124114064 1101 70-71 1.3014214900335972 1101 72-73 1.213081425652085 1101 74-75 1.1481720267667583 1101 76-77 1.0852089713291768 1101 78-79 1.2102795871158385 1101 80-81 1.252794920264101 1101 82-83 1.0761384315691558 1101 84-85 1.095298962376809 1101 86-87 1.0703737027315263 1101 88-89 1.1258494851933243 1101 90-91 1.0440745326909422 1101 92-93 1.161886571455753 1101 94-95 0.8063605875052282 1101 96-97 0.8782556204035359 1101 98-99 0.9552931633393698 1101 100 1.0834914995962919 1102 1 0.25763398737356624 1102 2 0.2349228435158217 1102 3 0.1305006519283438 1102 4 -0.14994182391688327 1102 5 0.08486915861281119 1102 6 -0.24398544223732443 1102 7 -0.08601947433907497 1102 8 0.13386238677225748 1102 9 -0.06959589220257101 1102 10-11 -0.14441601687762073 1102 12-13 -0.25336516070528603 1102 14-15 0.10061226280807034 1102 16-17 0.1473492195895716 1102 18-19 -0.0636627531105205 1102 20-21 -0.07680254223447491 1102 22-23 -0.17668068339825282 1102 24-25 -0.12630968723456704 1102 26-27 -0.31388857787450064 1102 28-29 -0.35371029089003514 1102 30-31 -0.18315402796676494 1102 32-33 -0.12253241190958164 1102 34-35 -0.07143109356074007 1102 36-37 -0.00765932366135047 1102 38-39 -0.036018054415926315 1102 40-41 -0.18477810268916528 1102 42-43 -0.13580892075054862 1102 44-45 -0.16722674824712414 1102 46-47 -0.2672799892034732 1102 48-49 -0.17539642715225057 1102 50-51 -0.14234686961540888 1102 52-53 -0.25169100227125796 1102 54-55 -0.29652361869126054 1102 56-57 -0.31984385258397907 1102 58-59 -0.32807360740036984 1102 60-61 -0.32268538918805234 1102 62-63 -0.4276882354436893 1102 64-65 -0.3205081515193413 1102 66-67 -0.5479998335031269 1102 68-69 -0.6909500013338672 1102 70-71 -0.8597701871416881 1102 72-73 -0.5476688896064275 1102 74-75 -0.5844099404463563 1102 76-77 -0.6486603014745214 1102 78-79 -0.7434982607193881 1102 80-81 -0.7231718725059721 1102 82-83 -0.8932657298432041 1102 84-85 -0.8448229375811565 1102 86-87 -1.0336773691474157 1102 88-89 -1.1799827972783028 1102 90-91 -0.9481438363716883 1102 92-93 -0.770101658893136 1102 94-95 -1.089525288913439 1102 96-97 -1.0833931816141131 1102 98-99 -0.9495880892877828 1102 100 -1.0229082481968987 1103 1 0.19047104279126614 1103 2 0.022557252117973547 1103 3 0.18614581321866552 1103 4 0.7199713274478796 1103 5 0.34328520493208003 1103 6 0.09482762641444964 1103 7 -0.07768614100574212 1103 8 0.4647143305274213 1103 9 -0.012627323137223812 1103 10-11 0.40565635698508373 1103 12-13 0.21519356551472413 1103 14-15 0.228197043618664 1103 16-17 0.11457419891132758 1103 18-19 0.11181284655862811 1103 20-21 0.3261751252593186 1103 22-23 0.15286853082837837 1103 24-25 0.3694926287290343 1103 26-27 0.3547735395779412 1103 28-29 0.3956176660992057 1103 30-31 0.49560941289345806 1103 32-33 0.5168182911507913 1103 34-35 0.47647006442105777 1103 36-37 0.5069188401103588 1103 38-39 0.715749935658522 1103 40-41 0.3812371992132313 1103 42-43 0.25850456146616807 1103 44-45 0.25170418640961145 1103 46-47 0.21909304636311333 1103 48-49 0.11401631064758533 1103 50-51 -0.0891520805335233 1103 52-53 -0.004968504339089463 1103 54-55 0.029551650125938522 1103 56-57 0.07270163955828224 1103 58-59 -0.04658063800418688 1103 60-61 -0.3552949838944244 1103 62-63 -0.29476846704005055 1103 64-65 -0.4128882176897335 1103 66-67 -0.2549270460755011 1103 68-69 -0.22838796659441485 1103 70-71 -0.01491907051637753 1103 72-73 -0.17390958439550985 1103 74-75 -0.23239381141409865 1103 76-77 -0.11079843216104024 1103 78-79 -0.09704457999151117 1103 80-81 0.005095290438610789 1103 82-83 -0.20440096557521414 1103 84-85 -0.06971954634873612 1103 86-87 -0.08346024755932646 1103 88-89 -0.1578363952931916 1103 90-91 -0.22083821188864405 1103 92-93 -0.10120174160612194 1103 94-95 0.06968686989549511 1103 96-97 0.1314021037456854 1103 98-99 0.3133047973954248 1103 100 0.1305036624730782 1104 1 0.13683449700885575 1104 2 0.27270752260485054 1104 3 -0.1063005903076828 1104 4 0.08805387603533887 1104 5 -0.013096281743933957 1104 6 0.20893301611591397 1104 7 0.03846292731724077 1104 8 0.525724148199231 1104 9 0.6344373933524565 1104 10-11 0.5757324940317687 1104 12-13 0.5672372441362441 1104 14-15 0.786151361390651 1104 16-17 0.5069805303078425 1104 18-19 0.6413460062460601 1104 20-21 0.5113484372208532 1104 22-23 0.6795265152002514 1104 24-25 0.2510394129406137 1104 26-27 0.392862098984871 1104 28-29 0.26132594099401985 1104 30-31 -0.0980091004305308 1104 32-33 0.025003023669327717 1104 34-35 0.32376519565728756 1104 36-37 0.41129672029469333 1104 38-39 0.4585770244182896 1104 40-41 0.4462041397061185 1104 42-43 0.43193673938642263 1104 44-45 0.182639870641232 1104 46-47 0.18496160977726106 1104 48-49 0.20786523871810658 1104 50-51 0.13109436306495326 1104 52-53 0.23458268780677827 1104 54-55 0.3947289693004521 1104 56-57 0.35423840573740506 1104 58-59 0.3588391175558314 1104 60-61 0.3001247716655939 1104 62-63 0.3936607054704737 1104 64-65 0.38815365768594745 1104 66-67 0.40770569285457015 1104 68-69 0.5141085270942227 1104 70-71 0.4771266117116326 1104 72-73 0.4823717219559249 1104 74-75 0.5658229788083027 1104 76-77 0.4066629984139922 1104 78-79 0.5453116612427102 1104 80-81 0.6097035853694877 1104 82-83 0.699155435947528 1104 84-85 0.6880644648714629 1104 86-87 0.796022821965817 1104 88-89 0.6078217894393276 1104 90-91 0.5430868532285658 1104 92-93 0.7165602299426652 1104 94-95 0.8133354373168551 1104 96-97 0.6668158484862161 1104 98-99 0.6576045369265806 1104 100 0.48395988367123266 1105 1 -0.09103817379859791 1105 2 -0.34841048981751044 1105 3 -0.47366601473832404 1105 4 -0.5936231425981973 1105 5 -0.5142608780172253 1105 6 -0.34036822612011264 1105 7 -0.398519474339075 1105 8 -0.353546038136173 1105 9 -0.515795525902206 1105 10-11 -0.34908634654794213 1105 12-13 -0.17758677242690624 1105 14-15 -0.5783025723567619 1105 16-17 -0.460022575282224 1105 18-19 -0.3799402256379949 1105 20-21 -0.14846005139198581 1105 22-23 -0.3133565075740776 1105 24-25 -0.27924009016497564 1105 26-27 -0.11723106871698974 1105 28-29 -0.23317457660431984 1105 30-31 -0.43761831368104254 1105 32-33 -0.43320091007808514 1105 34-35 -0.40682486645451377 1105 36-37 -0.21221518446721177 1105 38-39 -0.006485087382955612 1105 40-41 0.04118343577237482 1105 42-43 -0.06689866434028602 1105 44-45 0.014549808529430663 1105 46-47 -0.033076692500173976 1105 48-49 0.11443873768291013 1105 50-51 0.06037748935895593 1105 52-53 0.08072658014631884 1105 54-55 -0.00188076154840644 1105 56-57 0.09453343679330573 1105 58-59 0.11492089809412676 1105 60-61 0.23180179029913006 1105 62-63 -0.02429995705541188 1105 64-65 0.1859021048909142 1105 66-67 0.10309906759577103 1105 68-69 0.19229175690788836 1105 70-71 0.24931864069714038 1105 72-73 0.0955316598441236 1105 74-75 0.3932686309822131 1105 76-77 0.23870233588811374 1105 78-79 0.21243122646010093 1105 80-81 0.24809644251234175 1105 82-83 0.3758505705230988 1105 84-85 0.28349665948844205 1105 86-87 0.1439783085082631 1105 88-89 0.14625346645796 1105 90-91 -0.09569420267205331 1105 92-93 -0.0031602669517454274 1105 94-95 0.031697238559090124 1105 96-97 0.22899234952141256 1105 98-99 -0.15631885851855642 1105 100 -0.07258773537638419 1106 1 -0.12486322555843188 1106 2 -0.49768585213634964 1106 3 -0.08435442053542275 1106 4 -0.04734985067273101 1106 5 0.19125154434301805 1106 6 -0.10059079340789623 1106 7 -0.46464266274487187 1106 8 -0.3192033880326477 1106 9 0.003174453393860688 1106 10-11 0.07480081701313424 1106 12-13 -0.293735840749882 1106 14-15 0.16192775890617384 1106 16-17 -0.17324721296338197 1106 18-19 -0.08919229603136358 1106 20-21 0.0341227643430031 1106 22-23 0.04826357524165559 1106 24-25 0.19824438188472016 1106 26-27 0.41232379670744024 1106 28-29 0.242485361283876 1106 30-31 0.13749814594628162 1106 32-33 0.09839846880390724 1106 34-35 0.38939666563658193 1106 36-37 0.11533398737543621 1106 38-39 -0.009331878273229677 1106 40-41 -0.047584680169656224 1106 42-43 -0.07468851941274579 1106 44-45 0.19123200314640343 1106 46-47 0.015189353048484122 1106 48-49 0.1547596486560039 1106 50-51 0.19724343138793898 1106 52-53 0.051249354473441144 1106 54-55 0.22123000449713714 1106 56-57 0.015315010292276554 1106 58-59 -0.10586067540483413 1106 60-61 0.00716410913970833 1106 62-63 0.19438534315163025 1106 64-65 0.19043109039816386 1106 66-67 -3.688413069156127E-4 1106 68-69 0.038746208253645165 1106 70-71 0.060770504051177454 1106 72-73 0.05007358530995987 1106 74-75 0.1501915088290069 1106 76-77 0.009561549138631165 1106 78-79 0.022847893126769492 1106 80-81 -0.06835024485825514 1106 82-83 0.010232040502394568 1106 84-85 -0.18512394092563866 1106 86-87 -0.2716790413882215 1106 88-89 -0.20760264120249516 1106 90-91 -0.3196460660260918 1106 92-93 -0.14524101229335784 1106 94-95 -0.007989614442980297 1106 96-97 0.11101874703694392 1106 98-99 -0.1117536411272475 1106 100 -0.29740657595609576 1107 1 -0.0016011277937550972 1107 2 0.2928324480355968 1107 3 0.028099521984842113 1107 4 -0.14467237585082415 1107 5 0.06428028421037624 1107 6 0.4236047359460713 1107 7 0.029093520011208795 1107 8 0.032602065172952166 1107 9 -0.2782551707771006 1107 10-11 -0.1076113667255072 1107 12-13 0.07624696445768109 1107 14-15 0.37016897728004494 1107 16-17 0.36165821567823286 1107 18-19 0.3576757953466725 1107 20-21 0.24132001317621388 1107 22-23 0.16691992503286457 1107 24-25 0.07659122541210195 1107 26-27 -0.07939813893491277 1107 28-29 -0.28202949992958537 1107 30-31 -0.1541992257068756 1107 32-33 -0.3687840416357915 1107 34-35 -0.3322687728306235 1107 36-37 -0.23620126194905566 1107 38-39 -0.38209041425947277 1107 40-41 -0.2759069597078536 1107 42-43 -0.12304273213689498 1107 44-45 -0.4461543884035777 1107 46-47 -0.3994507845098596 1107 48-49 -0.6332408426237848 1107 50-51 -0.4369742055562966 1107 52-53 -0.5939606676341498 1107 54-55 -0.4356479124765755 1107 56-57 -0.34518609508724296 1107 58-59 -0.5936646547710822 1107 60-61 -0.33740724924889776 1107 62-63 -0.2957890611716323 1107 64-65 -0.37447925104128643 1107 66-67 -0.32173951190382155 1107 68-69 -0.40077725035603606 1107 70-71 -0.4105633205619412 1107 72-73 -0.4888357735699209 1107 74-75 -0.27261615513562276 1107 76-77 -0.239001457493643 1107 78-79 -0.45088092043255656 1107 80-81 -0.1540171571648159 1107 82-83 -0.20120249646641142 1107 84-85 0.10595933099772026 1107 86-87 -0.07863065032951155 1107 88-89 0.010206250961591223 1107 90-91 0.21213973598815627 1107 92-93 0.13374449495302088 1107 94-95 0.12383303355505504 1107 96-97 0.03435453676919664 1107 98-99 -0.03273128789708579 1107 100 0.20389531547107254 1108 1 0.016375246114822772 1108 2 0.07720934489323383 1108 3 -0.01378309462813121 1108 4 0.19809271729948108 1108 5 0.42845360446997915 1108 6 0.44246825558001035 1108 7 -0.03057870298921017 1108 8 0.08321606339865895 1108 9 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-0.2767111674519498 2215 28-29 -0.2770814211996111 2215 30-31 -0.16686533106251034 2215 32-33 -0.19921320052342395 2215 34-35 -0.26068941443353566 2215 36-37 -0.1819615333307283 2215 38-39 -0.2673999284813817 2215 40-41 -0.3372362906495141 2215 42-43 -0.13452825397311585 2215 44-45 -0.04104645805495721 2215 46-47 0.0797807074792587 2215 48-49 -0.04894961981991486 2215 50-51 -0.35136207147618137 2215 52-53 -0.24745787938262964 2215 54-55 -0.14704557693460885 2215 56-57 -0.2140711605531891 2215 58-59 -0.2553801921043686 2215 60-61 -0.39022844728186357 2215 62-63 -0.24083549135552573 2215 64-65 -0.45462165320843795 2215 66-67 -0.2831371763618513 2215 68-69 -0.3778062066835943 2215 70-71 -0.2112612986219986 2215 72-73 -0.4533633571259479 2215 74-75 -0.31861620394527534 2215 76-77 -0.19381591979428592 2215 78-79 -0.37254447548374614 2215 80-81 -0.310845627836315 2215 82-83 -0.3525093749669779 2215 84-85 -0.3524792481532373 2215 86-87 -0.2876335911111987 2215 88-89 -0.27628201641769223 2215 90-91 -0.48937092282015726 2215 92-93 -0.28544197012977435 2215 94-95 -0.6335006170410331 2215 96-97 -0.2983686758822657 2215 98-99 -0.32379546039040363 2215 100 -0.46698118390050425 2216 1 -0.4534485145039149 2216 2 -0.42163224713968717 2216 3 -0.4568424443059662 2216 4 -0.3818777749950897 2216 5 -0.6097853848911043 2216 6 -0.1763290748947668 2216 7 -0.36513383975050573 2216 8 -0.33647341410748055 2216 9 -0.2909374864521155 2216 10-11 -0.46525739655790943 2216 12-13 -0.6623945030505354 2216 14-15 -0.6488781352178847 2216 16-17 -0.6051585585458241 2216 18-19 -0.6745145930418559 2216 20-21 -0.8228598322248146 2216 22-23 -0.6469762525431904 2216 24-25 -0.9212753561542115 2216 26-27 -0.731621385513165 2216 28-29 -0.5591670551857177 2216 30-31 -0.4280982399611801 2216 32-33 -0.5043181645112398 2216 34-35 -0.8193323380624022 2216 36-37 -0.8645550430047706 2216 38-39 -0.6186339218121262 2216 40-41 -0.44225450007142086 2216 42-43 -0.5033526392356862 2216 44-45 -0.38604044849387975 2216 46-47 -0.2523808367520246 2216 48-49 -0.5939942170889552 2216 50-51 -0.7138990099437024 2216 52-53 -0.8148078888714139 2216 54-55 -0.5536714569060521 2216 56-57 -0.5566545488612107 2216 58-59 -0.571408550003099 2216 60-61 -0.5746835653494031 2216 62-63 -0.559541040936665 2216 64-65 -0.46707906665720245 2216 66-67 -0.5914366965006579 2216 68-69 -0.4926678964095039 2216 70-71 -0.5752915904245981 2216 72-73 -0.5002356742861807 2216 74-75 -0.43561286931664966 2216 76-77 -0.37394460573372257 2216 78-79 -0.5212246312804858 2216 80-81 -0.6234105807990744 2216 82-83 -1.0041242750636705 2216 84-85 -0.8331289133348321 2216 86-87 -0.8832307968912225 2216 88-89 -0.949495188652616 2216 90-91 -0.9033189785238172 2216 92-93 -1.0217645705978882 2216 94-95 -0.9588324984403123 2216 96-97 -0.7145285211699601 2216 98-99 -0.7921277845994474 2216 100 -0.8456874564363588 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8014.0 3 1262.0 4 1003.0 5 1056.0 6 2297.0 7 4603.0 8 4773.0 9 2607.0 10 1471.0 11 1546.0 12 1368.0 13 935.0 14 835.0 15 682.0 16 633.0 17 679.0 18 590.0 19 505.0 20 436.0 21 467.0 22 617.0 23 1081.0 24 1893.0 25 1599.0 26 1612.0 27 1363.0 28 1569.0 29 1816.0 30 2136.0 31 2690.0 32 3543.0 33 4925.0 34 7209.0 35 12808.0 36 20746.0 37 41578.0 38 68374.0 39 26912.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75787299081274 21.734913250138565 13.188414316666385 21.318799442382304 2 15.539564720897427 26.94632901188957 35.10781218512796 22.40629408208504 3 18.40164932523787 30.79384274305293 27.966727970506977 22.837779961202227 4 12.264772951566641 23.094981123042487 33.60728369190454 31.032962233486337 5 12.500472532226697 43.0886386451556 30.686452816082056 13.724436006535646 6 27.260776251296765 41.0049854468632 16.735477313486747 14.998760988353292 7 24.416909927126866 39.411554696851965 19.56696137933931 16.604573996681857 8 22.029647863362754 40.592537206850345 19.230785386938642 18.147029542848262 9 22.86401975939243 24.26490355534646 18.401774312789836 34.46930237247127 10-11 20.741112571987852 34.39853650955007 24.822839524323598 20.037511394138477 12-13 21.73324007385143 33.11929862148203 24.741698540825176 20.405762763841366 14-15 20.754388028600957 34.1478528277473 22.55917221909475 22.538586924557 16-17 19.478007786415354 36.626810635554605 23.985225129878586 19.909956448151462 18-19 19.763514932582854 35.44986768597471 25.457218465157304 19.329398916285125 20-21 21.391654608572203 33.87824019724172 25.06956592960033 19.660539264585744 22-23 21.346408604317695 33.99717416269349 24.37673072037857 20.279686512610244 24-25 21.18462116426324 33.8468286765462 24.394037017837316 20.574513141353236 26-27 20.49054739961538 33.498250296871554 25.033890646181657 20.977311657331413 28-29 20.422384399617044 34.120588877504744 25.40584006584141 20.0511866570368 30-31 20.588179708745 34.34667114643596 24.95196497380385 20.11318417101519 32-33 20.434087888414595 34.68041817231236 24.88561992008617 19.999874019186876 34-35 20.161859028174216 34.560638369320664 24.614871217972397 20.662631384532723 36-37 19.594786088117548 34.908185522556316 24.36636779114122 21.130660598184914 38-39 20.35524776841491 34.34278742878981 24.823037559503685 20.478927243291594 40-41 19.438068939449618 34.091114307613154 25.61952059468937 20.85129615824785 42-43 19.841423159945908 34.151345131405435 25.202840608437832 20.80439110021082 44-45 19.501517076294764 34.421778706338124 25.246669256370147 20.830034960996965 46-47 20.01360712778311 34.29521167693151 25.175076277610604 20.516104917674777 48-49 19.589352047255172 33.73314602612641 25.11134489514366 21.56615703147475 50-51 19.08451443569554 33.165774278215224 25.388766404199476 22.360944881889765 52-53 19.827312871532452 32.598685108464906 25.065881063420104 22.50812095658254 54-55 19.307495102578155 32.96547553808651 24.82599192476552 22.901037434569815 56-57 20.40124720481245 32.66437802483912 24.486367883426244 22.448006886922197 58-59 20.446881863113557 31.824480660454608 24.65555830838292 23.073079168048913 60-61 20.66834073787908 31.954884060770468 24.711516658128343 22.665258543222112 62-63 20.991706036745406 31.488923884514435 24.630551181102362 22.888818897637794 64-65 20.92593564642276 32.086294682693826 24.69560652984095 22.292163141042465 66-67 21.33741512024894 32.101658330918696 24.494920862886392 22.06600568594597 68-69 21.75166450751364 32.37007812844476 24.581379772270594 21.296877591771 70-71 22.15261361202904 31.982263800770077 24.546202715015724 21.318919872185155 72-73 22.264397328692205 31.727133592682243 25.034378076969105 20.974091001656443 74-75 22.401709868274637 32.272084048927766 24.515530323707626 20.810675759089975 76-77 22.574997008307456 31.47512486379926 24.824119970020092 21.12575815787319 78-79 22.900011756407242 30.54461556548319 25.297898962077188 21.25747371603238 80-81 23.147829372743303 30.548534721639097 25.481988412125283 20.821647493492318 82-83 23.10542680251343 30.27622128774503 25.621797065424367 20.996554844317174 84-85 23.160663418566234 30.13113453307332 25.597158576853403 21.11104347150705 86-87 22.995153059617994 29.995192943492942 25.645961337831114 21.363692659057943 88-89 23.159647001528207 29.488891128016526 25.558382454195844 21.793079416259424 90-91 23.258332861038166 29.603251070010938 25.42081499253774 21.71760107641316 92-93 23.193039170410177 29.835005667744234 25.164154666442755 21.80780049540283 94-95 22.379609940615758 29.940657739788286 25.629055246867583 22.05067707272838 96-97 21.809636166693256 30.679175098452472 25.443141074622353 22.068047660231922 98-99 21.625655472380945 31.451128734985534 25.088900737655706 21.83431505497781 100 20.99148500218333 32.01059756138524 25.135618554969604 21.862298881461825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3174.0 1 2065.0 2 838.0 3 630.0 4 441.0 5 283.5 6 222.0 7 239.0 8 255.5 9 239.5 10 183.0 11 125.0 12 113.0 13 121.0 14 131.5 15 134.5 16 154.0 17 188.5 18 201.0 19 261.5 20 343.0 21 441.0 22 605.5 23 813.5 24 1079.5 25 1382.5 26 1919.0 27 2467.0 28 3097.0 29 4064.5 30 4930.5 31 5279.0 32 5644.0 33 6211.5 34 6653.5 35 7094.5 36 6991.0 37 6923.5 38 7306.5 39 7542.0 40 7958.5 41 7962.5 42 7006.0 43 6453.5 44 6752.5 45 6981.5 46 7074.5 47 7408.5 48 7759.0 49 7658.5 50 7395.0 51 7179.5 52 6147.0 53 5548.0 54 5650.5 55 5858.0 56 6273.5 57 5791.0 58 4855.5 59 4127.0 60 3398.0 61 2685.0 62 2579.0 63 2324.5 64 1679.0 65 1214.0 66 712.0 67 454.0 68 438.0 69 392.0 70 300.0 71 235.0 72 202.0 73 168.5 74 145.5 75 127.0 76 67.0 77 23.5 78 17.0 79 22.0 80 20.5 81 10.0 82 9.5 83 6.0 84 2.0 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052459071432468384 2 0.03735085885991749 3 0.051619726289548896 4 0.06714761143355955 5 0.08477385943486893 6 0.07889844343443246 7 0.08225582400611042 8 0.09232796572114436 9 0.09106894800676513 10-11 0.09232796572114436 12-13 0.09883289057877045 14-15 0.10365912515055754 16-17 0.073023027433996 18-19 0.08855091257800664 20-21 0.08288533286330005 22-23 0.05099021743235927 24-25 0.018675429429958746 26-27 0.16094443115481302 28-29 0.055816452004146366 30-31 0.07281319114826612 32-33 0.0627410494332322 34-35 0.020563956001527606 36-37 0.04637381914630206 38-39 0.06945581057658815 40-41 0.07281319114826612 42-43 0.06840662914793878 44-45 0.06651810257636992 46-47 0.07155417343388688 48-49 0.07113450086242713 50-51 0.06546892114772054 52-53 0.06819679286220891 54-55 0.061062359147393205 56-57 0.06232137686177244 58-59 0.059593505147284086 60-61 0.05833448743290485 62-63 0.06546892114772054 64-65 0.06022301400447371 66-67 0.06295088571896207 68-69 0.06190170429031269 70-71 0.05203939886100865 72-73 0.05057054486089953 74-75 0.05623612457560612 76-77 0.05099021743235927 78-79 0.04742300057495142 80-81 0.04238692971743446 82-83 0.05057054486089953 84-85 0.03798036771710711 86-87 0.03756069514564737 88-89 0.03902954914575648 90-91 0.034622987145429135 92-93 0.038190204002836986 94-95 0.03630167743126813 96-97 0.039868894288675974 98-99 0.03944922171721623 100 0.04742300057495142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 238281.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.95424729625945 #Duplication Level Percentage of deduplicated Percentage of total 1 76.33866498614572 32.0273122909506 2 8.240554571917293 6.914525287370793 3 3.6081185167401895 4.5412768957659235 4 2.3397253148476027 3.926456578577394 5 1.6215026658264062 3.4014461916812504 6 1.2393842091048224 3.1198458962317597 7 0.896277846132301 2.6321863681955335 8 0.7682381538276866 2.5784682790486864 9 0.6051876081585292 2.285117151598323 >10 4.163290620092228 31.337370583470776 >50 0.16205023557302764 4.538339187765706 >100 0.016004961538076803 0.9287354006404203 >500 0.0 0.0 >1k 0.0010003100961298002 1.768919888702834 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4215 1.768919888702834 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 288 0.12086570058040717 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.196725714597471E-4 0.0 4 0.0 0.0 0.0 4.196725714597471E-4 0.0 5 0.0 0.0 0.0 4.196725714597471E-4 0.0 6 0.0 0.0 0.0 4.196725714597471E-4 0.0 7 0.0 0.0 0.0 4.196725714597471E-4 0.0 8 0.0 0.0 0.0 4.196725714597471E-4 0.0 9 0.0 0.0 0.0 4.196725714597471E-4 0.0 10-11 0.0 0.0 0.0 4.196725714597471E-4 0.0 12-13 0.0 0.0 0.0 4.196725714597471E-4 0.0 14-15 0.0 0.0 0.0 8.393451429194942E-4 0.0 16-17 0.0 0.0 0.0 0.001049181428649368 0.0 18-19 0.0 0.0 0.0 0.0014688540001091149 0.0 20-21 0.0 0.0 0.0 0.0027278717144883563 0.0 22-23 0.0 0.0 0.0 0.0054557434289767125 0.0 24-25 0.0 0.0 0.0 0.014688540001091148 0.0 26-27 0.0 0.0 0.0 0.03294429685959015 0.0 28-29 0.0 0.0 0.0 0.0650492485762608 0.0 30-31 0.0 0.0 0.0 0.13849194858171654 0.0 32-33 0.0 0.0 0.0 0.258937976590664 0.0 34-35 0.0 0.0 0.0 0.4666758994632388 0.0 36-37 0.0 0.0 0.0 0.7644335889139293 0.0 38-39 0.0 0.0 0.0 1.0997519735102674 0.0 40-41 0.0 0.0 0.0 1.4380080661068235 0.0 42-43 0.0 0.0 0.0 1.7233854146994516 0.0 44-45 0.0 0.0 0.0 1.984631590433144 0.0 46-47 0.0 0.0 0.0 2.2998056915994143 0.0 48-49 0.0 0.0 0.0 2.615189629051414 0.0 50-51 0.0 0.0 0.0 2.905812884787289 0.0 52-53 0.0 0.0 0.0 3.1811180916648834 0.0 54-55 0.0 0.0 0.0 3.51853483911852 0.0 56-57 0.0 0.0 0.0 3.9077811491474357 0.0 58-59 0.0 0.0 0.0 4.271637268603036 0.0 60-61 0.0 0.0 0.0 4.621014684343276 0.0 62-63 0.0 0.0 0.0 4.966195374368917 0.0 64-65 0.0 0.0 0.0 5.358169556112321 0.0 66-67 0.0 0.0 0.0 5.834497924719134 0.0 68-69 0.0 0.0 0.0 6.315023019040544 0.0 70-71 0.0 0.0 0.0 6.82009895879235 0.0 72-73 0.0 0.0 0.0 7.352033943117579 0.0 74-75 0.0 0.0 0.0 7.9032738657299575 0.0 76-77 0.0 0.0 0.0 8.502356461488745 0.0 78-79 0.0 0.0 0.0 9.205098182398093 0.0 80-81 0.0 0.0 0.0 9.866082482447194 0.0 82-83 0.0 4.196725714597471E-4 0.0 10.547001229640635 0.0 84-85 0.0 4.196725714597471E-4 0.0 11.276811831409134 0.0 86-87 0.0 4.196725714597471E-4 0.0 12.080484805754551 0.0 88 0.0 4.196725714597471E-4 0.0 12.701390375229247 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACAAC 170 0.0 41.47791 1 GTTGCCG 25 0.0016969234 37.61849 24-25 TAGTATG 50 0.0016995986 37.59874 7 TTAGTCT 65 1.408663E-4 36.16023 4 GAGAACA 320 0.0 35.25622 1 CTAGCAT 40 1.1608283E-5 35.23402 84-85 TAGTCTA 55 0.0027103564 34.18785 5 TATGGTC 60 0.004150831 31.338865 5 GTATAAG 90 2.582244E-5 31.338865 1 GGGTAGG 105 2.0534553E-6 31.338863 1 GTACATA 75 3.264426E-4 31.338861 1 ATAATCG 45 2.5950741E-5 31.325705 66-67 TAATCGG 45 2.5950741E-5 31.325705 68-69 TGCCGTG 80 4.7705736E-4 29.374016 7 GGGACGC 65 0.0061329086 28.93426 9 TGTACAG 65 0.006139208 28.928183 5 AGTCTAT 65 0.0061455127 28.922108 6 CATTACG 50 5.325911E-5 28.193134 48-49 TAGGAGT 85 6.7930983E-4 27.65194 4 CCGTATC 85 6.809805E-4 27.640327 94 >>END_MODULE