Basic Statistics
Measure | Value |
---|---|
Filename | SRR1033035_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 238281 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 3174 | 1.3320407418132374 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 3029 | 1.271188218951574 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1646 | 0.6907810526227437 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT | 1434 | 0.6018104674732774 | No Hit |
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 756 | 0.31727246402356885 | No Hit |
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 699 | 0.29335112745036324 | No Hit |
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 601 | 0.252223215447308 | No Hit |
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 576 | 0.24173140116081435 | No Hit |
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT | 535 | 0.22452482573096472 | TruSeq Adapter, Index 1 (95% over 21bp) |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG | 332 | 0.13933129372463604 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG | 330 | 0.13849194858171654 | No Hit |
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT | 303 | 0.12716078915230336 | RNA PCR Primer, Index 1 (95% over 23bp) |
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT | 276 | 0.1158296297228902 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC | 273 | 0.11457061200851096 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 269 | 0.11289192172267196 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG | 243 | 0.10198043486471854 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATCGTCT | 45 | 1.610484E-5 | 52.21989 | 7 |
TCGTCTT | 50 | 3.0029849E-5 | 46.997902 | 8 |
GGTATCA | 1835 | 0.0 | 46.198437 | 1 |
CATAGGT | 65 | 2.7764472E-6 | 43.382675 | 4 |
TTAATCG | 55 | 5.271835E-5 | 42.725365 | 4 |
TGATTCG | 50 | 0.0016996937 | 37.59832 | 5 |
GACCCTC | 70 | 2.1803992E-4 | 33.56993 | 7 |
GTTACGC | 35 | 2.1836626E-4 | 33.56993 | 80-81 |
TACGCAA | 35 | 2.1836626E-4 | 33.56993 | 82-83 |
GTATCAA | 2635 | 0.0 | 32.35108 | 2 |
CGGCCCT | 60 | 0.004155333 | 31.331932 | 9 |
TTCGTTC | 60 | 0.004155333 | 31.331932 | 8 |
ATTCGTT | 60 | 0.004155333 | 31.331932 | 7 |
ATAGACC | 75 | 3.26867E-4 | 31.331932 | 4 |
TAATCGT | 75 | 3.26867E-4 | 31.331932 | 5 |
TACTGGT | 80 | 4.71254E-4 | 29.435463 | 2 |
AGTTACG | 40 | 4.7779802E-4 | 29.37369 | 80-81 |
TCGTTCT | 65 | 0.006145856 | 28.921783 | 9 |
AACCTAC | 65 | 0.006145856 | 28.921783 | 7 |
GATTCGT | 65 | 0.006145856 | 28.921783 | 6 |