##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033035_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	238281
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59245596585544	34.0	31.0	34.0	31.0	34.0
2	32.562231147258906	34.0	31.0	34.0	31.0	34.0
3	32.61553376056001	34.0	31.0	34.0	31.0	34.0
4	36.17666116895598	37.0	35.0	37.0	35.0	37.0
5	36.18685921244245	37.0	35.0	37.0	35.0	37.0
6	36.28291806732388	37.0	37.0	37.0	35.0	37.0
7	36.22700509062829	37.0	37.0	37.0	35.0	37.0
8	36.248072653715575	37.0	37.0	37.0	35.0	37.0
9	38.00827174638347	39.0	39.0	39.0	35.0	39.0
10-11	37.97535053151531	39.0	38.5	39.0	35.0	39.0
12-13	37.93528229275519	39.0	38.0	39.0	35.0	39.0
14-15	39.29103873158162	41.0	39.0	41.0	36.0	41.0
16-17	39.29992110155656	41.0	39.0	41.0	36.0	41.0
18-19	39.40674245953307	41.0	39.0	41.0	36.0	41.0
20-21	39.41609486278806	41.0	39.0	41.0	36.0	41.0
22-23	39.28714836684419	41.0	39.0	41.0	35.5	41.0
24-25	39.17813421968181	41.0	39.0	41.0	35.0	41.0
26-27	38.968851901746255	41.0	39.0	41.0	35.0	41.0
28-29	38.81793974341219	40.5	38.0	41.0	35.0	41.0
30-31	38.557258027287105	40.0	38.0	41.0	35.0	41.0
32-33	38.186787868105306	40.0	37.0	41.0	34.5	41.0
34-35	37.89887989390677	40.0	36.0	41.0	34.0	41.0
36-37	37.62827082310382	40.0	36.0	41.0	33.5	41.0
38-39	37.34126094820821	40.0	35.5	41.0	33.0	41.0
40-41	37.069392859690865	40.0	35.0	41.0	33.0	41.0
42-43	36.730454799165685	40.0	35.0	41.0	32.5	41.0
44-45	36.203849656498	40.0	35.0	41.0	30.5	41.0
46-47	35.43735967198392	40.0	35.0	41.0	25.0	41.0
48-49	34.69799102740042	40.0	35.0	41.0	5.0	41.0
50-51	34.309307078617266	40.0	35.0	41.0	2.0	41.0
52-53	34.12204288214335	40.0	35.0	41.0	2.0	41.0
54-55	33.93168569881779	39.5	35.0	41.0	2.0	41.0
56-57	33.740207989726414	39.0	35.0	41.0	2.0	41.0
58-59	33.541457354971655	39.0	35.0	41.0	2.0	41.0
60-61	33.29850260826503	39.0	35.0	41.0	2.0	41.0
62-63	33.055268779298395	38.0	34.5	41.0	2.0	41.0
64-65	32.75670322014764	37.5	34.0	40.5	2.0	41.0
66-67	32.39920933687537	37.0	34.0	40.0	2.0	41.0
68-69	32.00771777858914	36.5	34.0	39.5	2.0	41.0
70-71	31.550732118800912	36.0	33.0	39.0	2.0	41.0
72-73	31.16532161607514	35.0	33.0	39.0	2.0	41.0
74-75	30.820531221540953	35.0	33.0	38.5	2.0	40.0
76-77	30.477870665306927	35.0	33.0	37.0	2.0	39.0
78-79	30.137169979981618	35.0	32.0	37.0	2.0	39.0
80-81	29.836071696862106	35.0	32.0	36.0	2.0	39.0
82-83	29.57162971449675	35.0	32.0	36.0	2.0	37.0
84-85	29.380640923951134	35.0	32.0	35.5	2.0	37.0
86-87	29.20719864361825	35.0	32.0	35.0	2.0	36.5
88-89	29.026663896827692	35.0	32.0	35.0	2.0	36.0
90-91	28.861646543366867	35.0	31.0	35.0	2.0	36.0
92-93	28.697848338726125	35.0	31.0	35.0	2.0	36.0
94-95	28.554248974949743	35.0	31.0	35.0	2.0	35.5
96-97	28.42473592103441	35.0	31.0	35.0	2.0	35.0
98-99	28.314842559834815	35.0	31.0	35.0	2.0	35.0
100	28.19953752082625	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21634927053590047
1101	2	-0.05612317229439867
1101	3	0.11965785841913856
1101	4	0.14899935577192736
1101	5	0.07205238190633168
1101	6	0.07771321872580472
1101	7	0.20838298958194912
1101	8	0.20130054803455266
1101	9	0.29582343491202323
1101	10-11	0.2515572116515301
1101	12-13	0.2161671937450791
1101	14-15	0.448071158576937
1101	16-17	0.4843935766074168
1101	18-19	0.34930851914135275
1101	20-21	0.32149319340017257
1101	22-23	0.3169067893152402
1101	24-25	0.42253453518720363
1101	26-27	0.6178306199933061
1101	28-29	0.6634858113278099
1101	30-31	0.5525131956897482
1101	32-33	0.518312043832708
1101	34-35	0.6072796003728698
1101	36-37	0.5672483031489719
1101	38-39	0.7040205934132047
1101	40-41	0.7368539034797905
1101	42-43	0.7316291407755457
1101	44-45	0.978683936048597
1101	46-47	1.3941182408478667
1101	48-49	1.0575502839715867
1101	50-51	1.3510739112094257
1101	52-53	1.1827208349648188
1101	54-55	1.1063798531226752
1101	56-57	0.9368224615254306
1101	58-59	1.21152965470813
1101	60-61	1.1208163404388145
1101	62-63	1.1467442634747727
1101	64-65	1.0384746786418901
1101	66-67	1.2535046182679181
1101	68-69	1.3673208567094548
1101	70-71	1.4212181784937883
1101	72-73	1.5228830242615778
1101	74-75	1.2689881458303311
1101	76-77	1.1169049424522157
1101	78-79	1.1862474126746072
1101	80-81	1.0193729393982167
1101	82-83	1.0949224608117802
1101	84-85	1.257146594165988
1101	86-87	1.1989168724796677
1101	88-89	1.2946833266730557
1101	90-91	1.299941984468692
1101	92-93	1.2370706713114927
1101	94-95	1.1101313654261844
1101	96-97	1.0053186468261295
1101	98-99	1.1334649255819613
1101	100	1.0085071648483392
1102	1	0.1554833604896615
1102	2	-0.16113578263487938
1102	3	-0.16358089736060322
1102	4	-0.012570631617734307
1102	5	0.037394962822681066
1102	6	0.12344672019701619
1102	7	0.050354406143526376
1102	8	-0.07546489963252867
1102	9	-0.03539766639104158
1102	10-11	0.058209166254300726
1102	12-13	0.25990405797374194
1102	14-15	0.34428280212464557
1102	16-17	0.23676326975579798
1102	18-19	0.3357261315835558
1102	20-21	0.18819664022656468
1102	22-23	0.3503084286595026
1102	24-25	0.4525103653679565
1102	26-27	0.4493827427339525
1102	28-29	0.46038576929334596
1102	30-31	0.4117134899310244
1102	32-33	0.3120331451357856
1102	34-35	0.2931980535044474
1102	36-37	0.5259599466966804
1102	38-39	0.8362084874863456
1102	40-41	0.7574980817059327
1102	42-43	0.4373826086191741
1102	44-45	0.16759209073543957
1102	46-47	0.17378932113369672
1102	48-49	0.27939769885178833
1102	50-51	-0.16510935907220414
1102	52-53	-0.21658034200029164
1102	54-55	-0.18986857058476403
1102	56-57	-0.32186291047961646
1102	58-59	-0.013811875346512181
1102	60-61	-0.1250747902883873
1102	62-63	-0.2876136600224868
1102	64-65	-0.10798917171540268
1102	66-67	-0.2534310689956456
1102	68-69	-0.14498473387482136
1102	70-71	0.047952100309679224
1102	72-73	0.042492103706724293
1102	74-75	0.2493580491510592
1102	76-77	0.1485568970549913
1102	78-79	0.25226254508318036
1102	80-81	0.020366003710950764
1102	82-83	0.028176979517116507
1102	84-85	0.03561443779776852
1102	86-87	0.057633770335908
1102	88-89	-0.04209683305792211
1102	90-91	-0.2757726014917985
1102	92-93	0.053774118137891236
1102	94-95	0.08566205058801657
1102	96-97	-0.12789383741094085
1102	98-99	-0.15742410342182112
1102	100	-0.3272431504101725
1103	1	-0.01035617631761454
1103	2	-0.01963454194009273
1103	3	0.03631571140697787
1103	4	-0.027521003826173285
1103	5	0.14529405297984255
1103	6	0.019807348815710668
1103	7	0.0749615194603166
1103	8	-0.016097653974966875
1103	9	0.07430043121027552
1103	10-11	0.1860627642691881
1103	12-13	0.054982635310381056
1103	14-15	0.08083160278635404
1103	16-17	0.38241049887076883
1103	18-19	0.42157187186477785
1103	20-21	0.4989389892753593
1103	22-23	0.4144213318853076
1103	24-25	0.42795494766737363
1103	26-27	0.5041077220557071
1103	28-29	0.6223171175150171
1103	30-31	0.34553276205674166
1103	32-33	0.21725440237317173
1103	34-35	0.1351748938683741
1103	36-37	0.3387184247777384
1103	38-39	0.3425980656501224
1103	40-41	0.34386077814927063
1103	42-43	0.47240121904101073
1103	44-45	0.5283137615377456
1103	46-47	-0.005749553969693011
1103	48-49	-0.10738890164449089
1103	50-51	-0.16862466097460072
1103	52-53	-0.45709936598705525
1103	54-55	-0.36511670954257625
1103	56-57	-0.1880849948468608
1103	58-59	-0.07604305814221135
1103	60-61	-0.20871829731898828
1103	62-63	-0.345006133140771
1103	64-65	-0.4032228359006851
1103	66-67	-0.3261357836358414
1103	68-69	-0.00166380748937911
1103	70-71	-0.02016204361091667
1103	72-73	0.06209508137421338
1103	74-75	-0.07231275316490482
1103	76-77	-0.019557246865606004
1103	78-79	0.16342879818491696
1103	80-81	0.09428039577050384
1103	82-83	0.06813769084879695
1103	84-85	0.13567647253722015
1103	86-87	0.20195759167586047
1103	88-89	0.29243583857152444
1103	90-91	0.16117942497635696
1103	92-93	0.22258098331572285
1103	94-95	0.3487617197360713
1103	96-97	0.2330552941027051
1103	98-99	0.42526820427048406
1103	100	0.4602258322201003
1104	1	-0.03379837227033278
1104	2	0.13909116433119806
1104	3	0.2903329424228005
1104	4	0.20535817870238304
1104	5	0.23850182697302813
1104	6	0.13475425324367762
1104	7	0.11202823955649421
1104	8	0.2443511538791725
1104	9	0.2377947209317739
1104	10-11	0.2719573745677266
1104	12-13	0.16868730371351148
1104	14-15	0.2708336063928414
1104	16-17	0.2157905830222404
1104	18-19	0.3914376302298379
1104	20-21	0.16909527058172102
1104	22-23	0.2249202299017412
1104	24-25	0.3517678108210447
1104	26-27	0.2884395192907263
1104	28-29	0.11879077008965311
1104	30-31	0.286185937773034
1104	32-33	0.30087491930364507
1104	34-35	0.4591480455413901
1104	36-37	0.6027436702195317
1104	38-39	0.6066934373191231
1104	40-41	0.2827708162177984
1104	42-43	0.2393116578308394
1104	44-45	0.44517411016528285
1104	46-47	0.6141162043953656
1104	48-49	0.2887741919239417
1104	50-51	0.3052282743089023
1104	52-53	0.48312303433351644
1104	54-55	0.4425370672556568
1104	56-57	0.5869701043316624
1104	58-59	0.43636928407376985
1104	60-61	0.5803520292688731
1104	62-63	0.48399607082637175
1104	64-65	0.45291821799633425
1104	66-67	0.25337733998667744
1104	68-69	0.45548906768593156
1104	70-71	0.28732142663552906
1104	72-73	0.35340944723276024
1104	74-75	0.38783551371547986
1104	76-77	0.5401001364243179
1104	78-79	0.6721144323263744
1104	80-81	0.5543184642938428
1104	82-83	0.6696704498149337
1104	84-85	0.7014648916916997
1104	86-87	0.66570233220882
1104	88-89	0.7217986917071677
1104	90-91	0.8180918673726687
1104	92-93	0.648026785760127
1104	94-95	0.49775991649023155
1104	96-97	0.48089139909168566
1104	98-99	0.37464708625806153
1104	100	0.3945676474875839
1105	1	0.2623972799035812
1105	2	0.3020052430061497
1105	3	0.15601617223646969
1105	4	0.1827132511661489
1105	5	0.19696455989227957
1105	6	0.1786207128710089
1105	7	0.2600613658504898
1105	8	0.2523739282062962
1105	9	0.3572305387371628
1105	10-11	0.3554848072799359
1105	12-13	0.31587933269901214
1105	14-15	0.5348734614653026
1105	16-17	0.2790022807448054
1105	18-19	0.5147554356128623
1105	20-21	0.3772992043291339
1105	22-23	0.4678679524690281
1105	24-25	0.4877667756243653
1105	26-27	0.7214752335764416
1105	28-29	0.6928720330296088
1105	30-31	0.9107977390152726
1105	32-33	0.7586906542932894
1105	34-35	0.7733949399513236
1105	36-37	0.847502986989717
1105	38-39	0.8577286340064916
1105	40-41	0.7760420377498818
1105	42-43	0.8428313265678966
1105	44-45	1.0265664497097902
1105	46-47	0.908564962159339
1105	48-49	1.3697136329177297
1105	50-51	1.0553576738948323
1105	52-53	0.7469040902341391
1105	54-55	0.5792477297815353
1105	56-57	0.6285720712053688
1105	58-59	0.7064262198915756
1105	60-61	0.5063354661218753
1105	62-63	0.6915530066441704
1105	64-65	0.6204906817644513
1105	66-67	0.6988354145208433
1105	68-69	0.6804502478101568
1105	70-71	0.352668217525796
1105	72-73	0.2956972319118485
1105	74-75	0.3047609795539863
1105	76-77	0.142261109509203
1105	78-79	0.1390528381234759
1105	80-81	0.30024237733732306
1105	82-83	0.43992148501162376
1105	84-85	0.44415381508714447
1105	86-87	0.23506051026264885
1105	88-89	0.319624778663691
1105	90-91	0.313627581658384
1105	92-93	0.48246001557378904
1105	94-95	0.3096776183535823
1105	96-97	0.27469315892605195
1105	98-99	0.29461344236572273
1105	100	0.44244549427847346
1106	1	0.001191275762792543
1106	2	-0.09817591641414225
1106	3	0.0220099610563409
1106	4	-0.0068571422085881295
1106	5	-0.027543721681219324
1106	6	-0.12694346932361356
1106	7	-0.06954525940830791
1106	8	-0.05999667220778093
1106	9	-0.17773322937878078
1106	10-11	-0.02069272895194274
1106	12-13	0.13431877369280443
1106	14-15	0.1053056560822867
1106	16-17	0.17013840910919953
1106	18-19	-0.04282738012212661
1106	20-21	0.020233986937824966
1106	22-23	-0.03574229597818146
1106	24-25	0.052803007508416044
1106	26-27	0.06701094786215123
1106	28-29	-0.06723407463085351
1106	30-31	-0.3020128199909422
1106	32-33	-0.21827621941271502
1106	34-35	-0.17480640580436813
1106	36-37	-0.011749083403650218
1106	38-39	-0.06939351920262027
1106	40-41	-0.38379233078013186
1106	42-43	-0.5216614270552924
1106	44-45	-0.16165818797136922
1106	46-47	-0.27304735668123925
1106	48-49	-0.2918676300222316
1106	50-51	0.09642910246626002
1106	52-53	0.2399553324701671
1106	54-55	0.20402775048547284
1106	56-57	0.06357465919708716
1106	58-59	0.22839826958102094
1106	60-61	0.35198764003491334
1106	62-63	0.23937909360070364
1106	64-65	0.44743167555326835
1106	66-67	0.473770714727884
1106	68-69	0.39027167638158744
1106	70-71	0.4817313645237249
1106	72-73	0.44243636234663697
1106	74-75	0.3402164660964324
1106	76-77	0.38088688590671893
1106	78-79	0.35265273460380797
1106	80-81	0.20131639390046985
1106	82-83	0.28933918687497595
1106	84-85	0.32454977782006367
1106	86-87	0.2700501582957777
1106	88-89	0.02925210785623733
1106	90-91	0.3039096727556938
1106	92-93	0.25899987064625307
1106	94-95	0.28057565147987873
1106	96-97	0.2800632417417894
1106	98-99	0.2672971897777323
1106	100	0.35709352740476774
1107	1	0.004679362228031891
1107	2	0.1236860339665995
1107	3	-0.0628094936230994
1107	4	0.08157321888204905
1107	5	0.055973841571052674
1107	6	0.009583182604664842
1107	7	-0.0010362935522536532
1107	8	-0.028800405934141793
1107	9	0.15913731839818013
1107	10-11	0.06717266038728553
1107	12-13	0.08204660469256453
1107	14-15	0.0902356487130831
1107	16-17	0.16709247444941155
1107	18-19	0.08189627499945829
1107	20-21	-0.006528479286686206
1107	22-23	0.08088752470469984
1107	24-25	0.2119899394661644
1107	26-27	0.1322651851502954
1107	28-29	-0.01891670789049016
1107	30-31	0.24034777937039564
1107	32-33	0.23296426204147735
1107	34-35	0.26842621517327814
1107	36-37	0.19105121136421843
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2216	46-47	-0.09391934746408737
2216	48-49	-0.10340725769793124
2216	50-51	-0.10036308721295484
2216	52-53	-0.05747053618546971
2216	54-55	-0.13414761789728402
2216	56-57	-0.12438544025109621
2216	58-59	0.008590495643723273
2216	60-61	-0.15928517544019627
2216	62-63	-0.3750439250155182
2216	64-65	-0.5831728966376204
2216	66-67	-0.42039276838411865
2216	68-69	-0.4538705830326393
2216	70-71	-0.16004596856705433
2216	72-73	-0.30663889082176254
2216	74-75	-0.36551696425951974
2216	76-77	-0.21425288730291214
2216	78-79	-0.27976191581157295
2216	80-81	-0.4175363136369725
2216	82-83	-0.5234738626671991
2216	84-85	-0.5451306539305882
2216	86-87	-0.6567007967706253
2216	88-89	-0.830853404241271
2216	90-91	-0.8914120178634306
2216	92-93	-0.9575242940496445
2216	94-95	-0.8014812280301555
2216	96-97	-0.7925824437836511
2216	98-99	-0.9590414191604779
2216	100	-1.0167346212823958
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	35.0
6	93.0
7	185.0
8	237.0
9	329.0
10	457.0
11	816.0
12	1301.0
13	1980.0
14	2190.0
15	2805.0
16	3529.0
17	5362.0
18	6407.0
19	2805.0
20	1183.0
21	906.0
22	879.0
23	996.0
24	1134.0
25	1432.0
26	1840.0
27	2325.0
28	1812.0
29	2116.0
30	2626.0
31	3132.0
32	3997.0
33	5010.0
34	7142.0
35	12317.0
36	20165.0
37	40666.0
38	70210.0
39	29754.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.001112589899364	19.47997967949014	14.404890357414256	21.11401737319624
2	16.531984973982105	22.008573075159536	39.03189032521317	22.42755162564519
3	18.362661943872048	27.0714336675382	32.62463435413405	21.941270034455695
4	11.954993557917886	19.765650902101335	36.59732160468698	31.682033935293795
5	11.563238361430413	37.530478720502266	35.33894855233947	15.56733436572786
6	27.700907751772068	38.43361409428364	17.447887158438984	16.417590995505307
7	23.670792048044113	35.90340816095283	22.227538074794044	18.198261716209014
8	24.82300160735944	33.09733547647926	21.549108398138316	20.53055451802299
9	25.30709540416567	15.095202722835644	21.7755507153319	37.82215115766679
10-11	23.070062972530046	26.920179537809748	27.953843926329903	22.055913563330304
12-13	25.720892559624982	25.164826402440816	29.33826028932227	19.776020748611934
14-15	22.715292932684235	25.4524089306698	29.606974987409767	22.225323149236193
16-17	18.538826007948597	28.979650077009918	30.811520851431712	21.670003063609773
18-19	18.594681478341702	25.15732508535109	36.617138204762874	19.630855231544338
20-21	19.777909275183504	23.546778803177762	37.439409772495495	19.23590214914324
22-23	19.355089159437807	24.325691095807052	37.2918948636274	19.027324881127743
24-25	19.703538318847393	24.263986872421594	37.31938038501408	18.71309442371693
26-27	19.017082807867197	24.01308541823607	37.87909627518828	19.090735498708455
28-29	18.88547555197435	23.771513465194456	38.341286128562494	19.0017248542687
30-31	19.589097723061684	23.919498238420687	37.68247926288555	18.80892477563208
32-33	19.66249931803207	23.706254380332464	37.69729856765751	18.933947733977952
34-35	19.226669352571125	23.917349683776717	37.582308283077545	19.273672680574617
36-37	19.253948069716007	24.069480990930874	36.74044510472929	19.936125834623827
38-39	19.621791078600477	23.519290249747147	36.89257641188345	19.96634225976893
40-41	18.95706950421877	23.15800075960895	37.22482536338475	20.660104372787533
42-43	20.114696513779947	23.303368711731107	36.313008590697535	20.268926183791404
44-45	19.806027337471306	24.230635258371418	35.61614228578863	20.34719511836865
46-47	20.768966052685695	24.093402327504084	34.328377000264396	20.80925461954583
48-49	20.997058095274067	24.181743403796357	33.074185520456936	21.747012980472636
50-51	20.19569332007168	24.531540492108057	33.02739202873918	22.245374159081084
52-53	20.720074198110634	24.506150301534742	32.706762184143926	22.067013316210694
54-55	20.021109530344425	25.402990586744224	31.789777615504384	22.78612226740697
56-57	20.65670363982021	25.25148878844725	30.953580016870834	23.138227554861697
58-59	19.889542179191793	24.870426093561804	31.6978693223547	23.5421624048917
60-61	20.369018092084556	25.534767774182583	30.96994724715777	23.126266886575095
62-63	19.673410804890025	25.48419722932168	31.300229560896586	23.5421624048917
64-65	19.16476764828081	25.95926658021412	31.402839504618495	23.473126266886577
66-67	19.324872734292704	25.806295927917038	30.973304627729448	23.89552671006081
68-69	19.553256672821888	25.810181299311736	31.232583515192207	23.403978512674165
70-71	19.82432469295641	25.18880059425761	31.359469197017802	23.627405515768178
72-73	20.121328104180176	24.675643519480275	31.459483505714925	23.743544870624625
74-75	20.150872922612052	25.462900788987746	30.547465167030385	23.838761121369817
76-77	20.44476899123304	25.149298517296803	30.537474662268497	23.868457829201656
78-79	20.477083779235443	24.77390140212606	30.761999487999464	23.987015330639036
80-81	20.506041186666163	25.160839513011947	30.68331088085076	23.649808419471128
82-83	20.438054230089683	25.224419907588096	30.55384189255543	23.783683969766788
84-85	20.57759536010005	25.317167545880704	30.12011028995178	23.985126804067466
86-87	20.535714285714285	25.442966258183645	29.916694644955516	24.10462481114655
88-89	20.511599114459287	25.06667785833447	29.72961630066416	24.69210672654209
90-91	20.45107827769349	24.900377087674457	29.764954978207815	24.883589656424235
92-93	20.399448544455332	24.924615523431616	29.55092465329436	25.125011278818693
94-95	20.0690785401178	24.58940026313636	30.11232607085377	25.229195125892073
96-97	19.939105382102188	24.737441796538114	29.83479554558598	25.48865727577372
98-99	20.441454504250242	24.61720535251521	29.584880004868214	25.356460138366337
100	20.453162442662233	24.60792090011373	29.69729017420609	25.24162648301795
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	233.0
1	166.5
2	280.0
3	456.0
4	279.0
5	159.5
6	331.5
7	662.5
8	1089.5
9	1216.0
10	779.5
11	328.5
12	214.5
13	208.5
14	201.0
15	190.0
16	205.5
17	221.0
18	236.0
19	266.0
20	303.0
21	369.5
22	489.0
23	658.0
24	861.0
25	1106.5
26	1607.0
27	2213.5
28	2904.0
29	3935.0
30	5004.0
31	5450.5
32	5909.5
33	6582.0
34	7113.0
35	7610.5
36	7441.5
37	7213.5
38	7475.0
39	7627.5
40	8011.0
41	8079.5
42	7077.0
43	6661.5
44	6962.5
45	7086.5
46	7277.5
47	7714.5
48	8006.5
49	7802.0
50	7663.5
51	7239.0
52	5947.5
53	5301.5
54	5347.0
55	5600.0
56	6052.5
57	5652.5
58	4780.0
59	4130.0
60	3435.5
61	2666.5
62	2475.0
63	2249.5
64	1622.0
65	1124.5
66	638.0
67	378.0
68	389.0
69	314.5
70	225.5
71	201.5
72	182.5
73	149.0
74	128.5
75	110.5
76	52.5
77	14.0
78	4.0
79	10.5
80	12.0
81	9.0
82	11.0
83	5.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04112791200305521
2	0.2350166400174584
3	0.0016786902858389884
4	0.0016786902858389884
5	0.0
6	0.0
7	0.0
8	8.393451429194942E-4
9	0.0
10-11	0.001049181428649368
12-13	0.0
14-15	4.196725714597471E-4
16-17	0.0
18-19	2.0983628572987355E-4
20-21	0.0
22-23	0.0
24-25	0.0016786902858389884
26-27	2.0983628572987355E-4
28-29	0.0
30-31	2.0983628572987355E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	2.0983628572987355E-4
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	4.196725714597471E-4
70-71	2.0983628572987355E-4
72-73	6.295088571896207E-4
74-75	4.196725714597471E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	4.196725714597471E-4
88-89	0.0035672168574078502
90-91	0.0031475442859481033
92-93	0.001049181428649368
94-95	6.295088571896207E-4
96-97	2.0983628572987355E-4
98-99	2.0983628572987355E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	238281.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.484994607207454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.16808193580434	25.60883998304523
2	9.526456465690565	6.7609251262165255
3	4.187856281193084	4.4581817266168935
4	2.768644889656314	3.929813959149072
5	1.9455022825649881	3.4518069002564196
6	1.4050192776214017	2.9914260893650773
7	1.0963407999621544	2.723255316202299
8	0.8881897958700948	2.5213928093301607
9	0.7237978096837524	2.311556523600287
>10	5.010998888284409	32.318145382972205
>50	0.2365352319227949	5.660963316420529
>100	0.031932256309577314	2.0387693521514514
>500	0.0059133807980698725	1.3291030338130192
>1k	0.004730704638455898	3.895820480860832
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3174	1.3320407418132374	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3029	1.271188218951574	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1646	0.6907810526227437	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	1434	0.6018104674732774	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	756	0.31727246402356885	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	699	0.29335112745036324	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	601	0.252223215447308	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	576	0.24173140116081435	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	535	0.22452482573096472	TruSeq Adapter, Index 1 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	332	0.13933129372463604	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	330	0.13849194858171654	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT	303	0.12716078915230336	RNA PCR Primer, Index 1 (95% over 23bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	276	0.1158296297228902	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	273	0.11457061200851096	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	269	0.11289192172267196	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	243	0.10198043486471854	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.196725714597471E-4	0.0
4	0.0	0.0	0.0	4.196725714597471E-4	0.0
5	0.0	0.0	0.0	4.196725714597471E-4	0.0
6	0.0	0.0	0.0	4.196725714597471E-4	0.0
7	0.0	0.0	0.0	4.196725714597471E-4	0.0
8	0.0	0.0	0.0	4.196725714597471E-4	0.0
9	0.0	0.0	0.0	4.196725714597471E-4	0.0
10-11	0.0	0.0	0.0	4.196725714597471E-4	0.0
12-13	0.0	0.0	0.0	4.196725714597471E-4	0.0
14-15	0.0	0.0	0.0	8.393451429194942E-4	0.0
16-17	0.0	0.0	0.0	0.001049181428649368	0.0
18-19	0.0	0.0	0.0	0.0018885265715688619	0.0
20-21	0.0	0.0	0.0	0.0031475442859481033	0.0
22-23	0.0	0.0	0.0	0.0058754160004364595	0.0
24-25	0.0	0.0	0.0	0.01594755771547039	0.0
26-27	0.0	0.0	0.0	0.03420331457396939	0.0
28-29	0.0	0.0	0.0	0.06693777514782967	0.0
30-31	0.0	0.0	0.0	0.1405903114390153	0.0
32-33	0.0	0.0	0.0	0.2618756845908822	0.0
34-35	0.0	0.0	0.0	0.47129229774929604	0.0
36-37	0.0	0.0	0.0	0.7698893323429061	0.0
38-39	0.0	0.0	0.0	1.1062568983678933	0.0
40-41	0.0	0.0	0.0	1.4440933183929898	0.0
42-43	0.0	0.0	0.0	1.732828047557296	0.0
44-45	0.0	0.0	0.0	1.9965922587197469	0.0
46-47	0.0	0.0	0.0	2.3134450501718558	0.0
48-49	0.0	0.0	0.0	2.6315568593383443	0.0
50-51	0.0	0.0	0.0	2.926586677074546	0.0
52-53	0.0	0.0	0.0	3.2065082822381976	0.0
54-55	0.0	0.0	0.0	3.5479119191207023	0.0
56-57	0.0	0.0	0.0	3.9453418442930825	0.0
58-59	0.0	0.0	0.0	4.314024198320471	0.0
60-61	0.0	0.0	0.0	4.668857357489687	0.0
62-63	0.0	0.0	0.0	5.0236905166589025	0.0
64-65	0.0	0.0	0.0	5.420281096688363	0.0
66-67	0.0	0.0	0.0	5.9043734078671815	0.0
68-69	0.0	0.0	0.0	6.394970643903626	0.0
70-71	0.0	0.0	0.0	6.910958070513386	0.0
72-73	0.0	0.0	0.0	7.446040599124563	0.0
74-75	0.0	0.0	0.0	8.010290371452193	0.0
76-77	0.0	0.0	0.0	8.620284454068937	0.0
78-79	0.0	0.0	0.0	9.334986843264886	0.0
80-81	0.0	0.0	0.0	10.00457443102891	0.0
82-83	0.0	0.0	0.0	10.691998103079978	0.0
84-85	0.0	0.0	0.0	11.429572647420482	0.0
86-87	0.0	0.0	0.0	12.240589891766444	0.0
88	0.0	0.0	0.0	12.868000386098766	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTCT	45	1.610484E-5	52.21989	7
TCGTCTT	50	3.0029849E-5	46.997902	8
GGTATCA	1835	0.0	46.198437	1
CATAGGT	65	2.7764472E-6	43.382675	4
TTAATCG	55	5.271835E-5	42.725365	4
TGATTCG	50	0.0016996937	37.59832	5
GACCCTC	70	2.1803992E-4	33.56993	7
GTTACGC	35	2.1836626E-4	33.56993	80-81
TACGCAA	35	2.1836626E-4	33.56993	82-83
GTATCAA	2635	0.0	32.35108	2
CGGCCCT	60	0.004155333	31.331932	9
TTCGTTC	60	0.004155333	31.331932	8
ATTCGTT	60	0.004155333	31.331932	7
ATAGACC	75	3.26867E-4	31.331932	4
TAATCGT	75	3.26867E-4	31.331932	5
TACTGGT	80	4.71254E-4	29.435463	2
AGTTACG	40	4.7779802E-4	29.37369	80-81
TCGTTCT	65	0.006145856	28.921783	9
AACCTAC	65	0.006145856	28.921783	7
GATTCGT	65	0.006145856	28.921783	6
>>END_MODULE