FastQCFastQC Report
Wed 25 May 2016
SRR1033034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3414263
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC37550.033.179774
GTATCAA41200.032.2981451
CAACGCA39450.031.5817555
ATCAACG39500.031.541783
GGTATCA26600.031.464931
AACGCAG40550.030.9501156
GTACATG88500.028.3186361
TACATGG88350.026.554092
TATCAAC49950.025.9783172
ACATGGG89450.025.1763383
ACGCAGA50350.024.5526397
CGCAGAG53850.023.1510948
CATGGGG66550.020.5580714
GAGTACA65250.019.6729971
AGTACAT64850.017.8345662
GCAGAGT67450.017.7885159
ACGACCG2556.0777147E-616.5935655
AGAGTAC53950.015.618319510-11
TATAGCG1850.00336742615.2481425
CGAGCGT1850.00337236115.2447886
Produced by FastQC (version 0.11.5)