FastQCFastQC Report
Wed 25 May 2016
SRR1033034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3414263
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49860.1460344443295669No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40620.11897150278112728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44250.049.8431131
GTATCAA78450.042.2313541
TCAACGC90850.035.6943444
ATCAACG92200.035.274693
CAACGCA93400.034.720335
AACGCAG96900.033.5632446
TATCAAC98650.033.0486762
ACGCAGA113550.028.4348687
CGCAGAG116000.027.753688
GTACATG86350.026.9610771
TACATGG87700.025.3026712
GCAGAGT131500.024.1606679
ACATGGG89500.023.4206943
GAGTACT91100.020.91978812-13
GTGGTAT21900.020.873741
TATACCG1605.4184282E-520.561735
CAGAGTA127400.020.53005210-11
AGAGTAC119050.020.19345310-11
GAGTACA63700.019.9014841
CATGGGG66000.019.2974824
Produced by FastQC (version 0.11.5)