##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033034_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3414263 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69298645124878 34.0 31.0 34.0 31.0 34.0 2 32.69783229938643 34.0 31.0 34.0 31.0 34.0 3 32.80433112504807 34.0 31.0 34.0 31.0 34.0 4 36.29455961652632 37.0 37.0 37.0 35.0 37.0 5 36.27729586150803 37.0 37.0 37.0 35.0 37.0 6 36.34221616788162 37.0 37.0 37.0 35.0 37.0 7 36.2900628920502 37.0 37.0 37.0 35.0 37.0 8 36.30255050650755 37.0 37.0 37.0 35.0 37.0 9 38.10225632881826 39.0 39.0 39.0 37.0 39.0 10-11 38.067086659697864 39.0 39.0 39.0 35.0 39.0 12-13 38.039676498266246 39.0 39.0 39.0 35.0 39.0 14-15 39.53321683186093 41.0 39.0 41.0 37.0 41.0 16-17 39.528357950163766 41.0 39.0 41.0 37.0 41.0 18-19 39.541802286467096 41.0 39.5 41.0 37.0 41.0 20-21 39.53600162026183 41.0 40.0 41.0 37.0 41.0 22-23 39.46529909968857 41.0 39.0 41.0 36.0 41.0 24-25 39.41033511478173 41.0 39.0 41.0 36.0 41.0 26-27 39.29786442930729 41.0 39.0 41.0 36.0 41.0 28-29 39.2477515645397 41.0 39.0 41.0 36.0 41.0 30-31 39.25293233122346 41.0 39.0 41.0 36.0 41.0 32-33 39.216766839578554 41.0 39.0 41.0 35.5 41.0 34-35 39.14068687737295 41.0 39.0 41.0 35.0 41.0 36-37 39.0527102042227 41.0 39.0 41.0 35.0 41.0 38-39 38.96340059333449 41.0 39.0 41.0 35.0 41.0 40-41 38.86319038691512 41.0 39.0 41.0 35.0 41.0 42-43 38.75054177138668 41.0 39.0 41.0 35.0 41.0 44-45 38.59206262083501 41.0 38.0 41.0 35.0 41.0 46-47 38.40149411454243 40.5 38.0 41.0 34.5 41.0 48-49 38.2087485644779 40.0 38.0 41.0 34.0 41.0 50-51 38.0725077417879 40.0 38.0 41.0 34.0 41.0 52-53 37.93498113648538 40.0 38.0 41.0 34.0 41.0 54-55 37.78569840694756 40.0 37.0 41.0 33.0 41.0 56-57 37.631720081317695 40.0 37.0 41.0 33.0 41.0 58-59 37.44715155217978 40.0 36.5 41.0 33.0 41.0 60-61 37.249999194555315 40.0 36.0 41.0 33.0 41.0 62-63 37.014324614126096 39.0 35.5 41.0 32.5 41.0 64-65 36.744413215970766 39.0 35.0 41.0 32.0 41.0 66-67 36.46383011502043 38.5 35.0 41.0 32.0 41.0 68-69 36.15547630630681 37.5 35.0 40.0 31.5 41.0 70-71 35.825793736452056 37.0 35.0 40.0 31.0 41.0 72-73 35.47782976882566 37.0 35.0 39.0 31.0 41.0 74-75 35.130507667394106 36.0 35.0 39.0 31.0 41.0 76-77 34.77834879738321 36.0 35.0 39.0 31.0 40.5 78-79 34.42240814489101 35.0 34.5 37.0 30.0 39.5 80-81 34.093761230461745 35.0 34.0 37.0 30.0 39.0 82-83 33.79537487299602 35.0 34.0 36.5 30.0 39.0 84-85 33.57454229507218 35.0 34.0 36.0 30.0 37.0 86-87 33.36900672268071 35.0 34.0 36.0 30.0 37.0 88-89 33.164117263374266 35.0 34.0 35.5 30.0 37.0 90-91 32.98397487246882 35.0 34.0 35.0 30.0 36.0 92-93 32.81058079005631 35.0 34.0 35.0 29.0 36.0 94-95 32.65653553929501 35.0 34.0 35.0 29.0 36.0 96-97 32.51309506619731 35.0 34.0 35.0 29.0 36.0 98-99 32.39038937539375 35.0 34.0 35.0 29.0 35.5 100 32.28664399901238 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18042883420654476 1101 2 0.21224012882694865 1101 3 0.2197534349896344 1101 4 0.16873228408915963 1101 5 0.17300926770800373 1101 6 0.17100073489368128 1101 7 0.17823864634506492 1101 8 0.15146963285352655 1101 9 0.2168246685711921 1101 10-11 0.27043923209510723 1101 12-13 0.23949276476656678 1101 14-15 0.3911772033264569 1101 16-17 0.4003423223178686 1101 18-19 0.4075190680812568 1101 20-21 0.3918490734645488 1101 22-23 0.36266029969866764 1101 24-25 0.38840771446264455 1101 26-27 0.38719137213366395 1101 28-29 0.41364892768465467 1101 30-31 0.36487223982560124 1101 32-33 0.3570822706073855 1101 34-35 0.3482357302735011 1101 36-37 0.3069368991059136 1101 38-39 0.3051055743824662 1101 40-41 0.3615044866172923 1101 42-43 0.4120375244713017 1101 44-45 0.35502850199436153 1101 46-47 0.35435832214101737 1101 48-49 0.37920243961362843 1101 50-51 0.3746230769703658 1101 52-53 0.4267520647338259 1101 54-55 0.4587232556792671 1101 56-57 0.39551571562361687 1101 58-59 0.37026043538857323 1101 60-61 0.3777996611484724 1101 62-63 0.3868511363162028 1101 64-65 0.3659775911556977 1101 66-67 0.4342524093914335 1101 68-69 0.41929002040307495 1101 70-71 0.4307648532930841 1101 72-73 0.4032066417786524 1101 74-75 0.4245553367690462 1101 76-77 0.3883939553396871 1101 78-79 0.3753180735884314 1101 80-81 0.43620142964566355 1101 82-83 0.42820162409279305 1101 84-85 0.40510542797476745 1101 86-87 0.4410496494658531 1101 88-89 0.4924175727484297 1101 90-91 0.4892584063170844 1101 92-93 0.4637040122906342 1101 94-95 0.5360492184930337 1101 96-97 0.5752481218163581 1101 98-99 0.5106783620742306 1101 100 0.5401561934633534 1102 1 0.12113477178778709 1102 2 0.09905305980960577 1102 3 0.1072908208028025 1102 4 0.04306761018381877 1102 5 0.09790298306234746 1102 6 0.09760689375529097 1102 7 0.10458251579922972 1102 8 0.11037252029614564 1102 9 0.1626928254501223 1102 10-11 0.19367612078369945 1102 12-13 0.16707834482245687 1102 14-15 0.2237760625015497 1102 16-17 0.24650647375037238 1102 18-19 0.29685045600575677 1102 20-21 0.25127678860983593 1102 22-23 0.2531876890307245 1102 24-25 0.2601517839459859 1102 26-27 0.3109437935243804 1102 28-29 0.33142665483398304 1102 30-31 0.30047827577494957 1102 32-33 0.34302154812068153 1102 34-35 0.3395603166699104 1102 36-37 0.2872688790003721 1102 38-39 0.31471589656192833 1102 40-41 0.3209935047321153 1102 42-43 0.31077452439356534 1102 44-45 0.32752946787032045 1102 46-47 0.31960679444880924 1102 48-49 0.3370333866238937 1102 50-51 0.37945561571357445 1102 52-53 0.3491541743064701 1102 54-55 0.2832466643387974 1102 56-57 0.3075575371910091 1102 58-59 0.34324773751801274 1102 60-61 0.35662783248587004 1102 62-63 0.3333604184894412 1102 64-65 0.39579539765810523 1102 66-67 0.358251612567706 1102 68-69 0.38359635681737814 1102 70-71 0.3715271256613022 1102 72-73 0.3880122355501854 1102 74-75 0.43564625393575795 1102 76-77 0.3759474135585208 1102 78-79 0.4559536391131118 1102 80-81 0.3633921396994637 1102 82-83 0.3846213775101148 1102 84-85 0.44012087241746656 1102 86-87 0.43400100454482526 1102 88-89 0.4736883381242407 1102 90-91 0.4048188307128342 1102 92-93 0.3986606905044141 1102 94-95 0.40848859422126793 1102 96-97 0.38616928795889294 1102 98-99 0.4092392260440576 1102 100 0.3870567176209718 1103 1 0.1429966166404526 1103 2 0.028737995357808188 1103 3 0.07077958110768634 1103 4 0.0783508668947519 1103 5 0.11151907402410899 1103 6 0.09984694961314489 1103 7 0.11858251875310799 1103 8 0.12927531662284508 1103 9 0.11959397351221668 1103 10-11 0.17271705527293335 1103 12-13 0.12450246797263276 1103 14-15 0.22194419583079394 1103 16-17 0.22709565634922058 1103 18-19 0.24156048291190757 1103 20-21 0.25720559347398364 1103 22-23 0.2288691397398921 1103 24-25 0.20439255944266677 1103 26-27 0.18404204914792643 1103 28-29 0.2855254945871408 1103 30-31 0.23729516812414175 1103 32-33 0.23682774336545975 1103 34-35 0.24093818133276557 1103 36-37 0.2161254821943217 1103 38-39 0.21068811606617288 1103 40-41 0.2103463577917566 1103 42-43 0.20678666742188057 1103 44-45 0.23290286959088036 1103 46-47 0.28983935336116673 1103 48-49 0.20293622319720583 1103 50-51 0.21916335890183802 1103 52-53 0.19149534698915005 1103 54-55 0.16635880134857928 1103 56-57 0.15622033452203254 1103 58-59 0.13763166538723937 1103 60-61 0.14549848322177183 1103 62-63 0.14040374968244862 1103 64-65 0.09785513765027076 1103 66-67 0.1292469092534887 1103 68-69 0.14342668230164435 1103 70-71 0.1630224365626276 1103 72-73 0.19465776228776832 1103 74-75 0.22116415942860357 1103 76-77 0.19313048908588115 1103 78-79 0.10121715837222212 1103 80-81 0.07192775222140568 1103 82-83 0.0568281985342054 1103 84-85 0.120351167919857 1103 86-87 0.0865668825134378 1103 88-89 0.11259446310360488 1103 90-91 0.11516586352267666 1103 92-93 0.13303591550693739 1103 94-95 0.1322108845263017 1103 96-97 0.1535035282562376 1103 98-99 0.1158782634781943 1103 100 0.09045253411565568 1104 1 0.08116357342543523 1104 2 0.02206403912659738 1104 3 0.06766025788694918 1104 4 0.06497728570916905 1104 5 0.08653740819630684 1104 6 0.08733887190413014 1104 7 0.08001610018214933 1104 8 0.08621210823003622 1104 9 0.11236650787257929 1104 10-11 0.14788472672456976 1104 12-13 0.15545224424898407 1104 14-15 0.22478363293951276 1104 16-17 0.21883588463678905 1104 18-19 0.23403729770386406 1104 20-21 0.21496647458686624 1104 22-23 0.21355929002454133 1104 24-25 0.2376621355488453 1104 26-27 0.24476688272903147 1104 28-29 0.2480723135443128 1104 30-31 0.22523003025487753 1104 32-33 0.22970652506963773 1104 34-35 0.21213421328336324 1104 36-37 0.22302983947331967 1104 38-39 0.24033853451928167 1104 40-41 0.2570177859624607 1104 42-43 0.2137842612657579 1104 44-45 0.23636819132229903 1104 46-47 0.24908425714932747 1104 48-49 0.21446990382572295 1104 50-51 0.2817790776516702 1104 52-53 0.23288942681129043 1104 54-55 0.2559879844463211 1104 56-57 0.2465866774805221 1104 58-59 0.17687071917635677 1104 60-61 0.28187142560015843 1104 62-63 0.19604533809479818 1104 64-65 0.2473653501855324 1104 66-67 0.24532343345079966 1104 68-69 0.25099305447484 1104 70-71 0.20186772684589727 1104 72-73 0.27229788749468753 1104 74-75 0.2125521263898733 1104 76-77 0.2474261013925485 1104 78-79 0.246212321890809 1104 80-81 0.2189702389861381 1104 82-83 0.18038671449035348 1104 84-85 0.20790965247372384 1104 86-87 0.2725164056308955 1104 88-89 0.24288541763962002 1104 90-91 0.18685785754495043 1104 92-93 0.23787584587854838 1104 94-95 0.24835612327807155 1104 96-97 0.24577875095225465 1104 98-99 0.2588348364090365 1104 100 0.22601982260052722 1105 1 -0.011255115182663644 1105 2 -0.008989259722739007 1105 3 0.03134266282692977 1105 4 0.007039573686782319 1105 5 0.007231766089589087 1105 6 0.03198787911335188 1105 7 0.0384874819212655 1105 8 0.018307309699331142 1105 9 0.07695967154437966 1105 10-11 0.07054481673330315 1105 12-13 0.1138669971981301 1105 14-15 0.08010235990418124 1105 16-17 0.07817450063922138 1105 18-19 0.07316144915290579 1105 20-21 0.06562033156955493 1105 22-23 0.08877896778239602 1105 24-25 0.04814772364764508 1105 26-27 0.05638131332194263 1105 28-29 0.07624584127128031 1105 30-31 0.0781031065958473 1105 32-33 0.05171682697910995 1105 34-35 0.04820309469780426 1105 36-37 0.02307521801035506 1105 38-39 0.03208074818650175 1105 40-41 0.07009458164881721 1105 42-43 0.06963689285177566 1105 44-45 0.08452656434612749 1105 46-47 0.05461868527236646 1105 48-49 0.08105935399120767 1105 50-51 0.08726514059335244 1105 52-53 0.1028352024377881 1105 54-55 0.1473895277024866 1105 56-57 0.1263546957547561 1105 58-59 0.09959322669582349 1105 60-61 0.07125465166598133 1105 62-63 0.16940111488575127 1105 64-65 0.16850657215260867 1105 66-67 0.10539549715156937 1105 68-69 0.16367775241938887 1105 70-71 0.16648144467109915 1105 72-73 0.17750611700856922 1105 74-75 0.13613656604002244 1105 76-77 0.1593403122154058 1105 78-79 0.24297172889761498 1105 80-81 0.23472814361016248 1105 82-83 0.2733492911657436 1105 84-85 0.2737164172566864 1105 86-87 0.19485190780493866 1105 88-89 0.17071412217760695 1105 90-91 0.16570704643611833 1105 92-93 0.12966829557456094 1105 94-95 0.0405581207726442 1105 96-97 0.06683403977921643 1105 98-99 0.04482876647391265 1105 100 0.03621614245066951 1106 1 -0.0030489176477317415 1106 2 0.02068124081300482 1106 3 0.056416383710534035 1106 4 0.020524365282412305 1106 5 0.020090297342257202 1106 6 0.007852191820163057 1106 7 0.013560628107640582 1106 8 0.022373452242128167 1106 9 0.041052428954458264 1106 10-11 0.0837065805940469 1106 12-13 0.051486246873423624 1106 14-15 0.07358178127346804 1106 16-17 0.0403206308196431 1106 18-19 0.07079393602107587 1106 20-21 0.0808047902239295 1106 22-23 0.09570382995084259 1106 24-25 0.054530135485890696 1106 26-27 0.050814971550771304 1106 28-29 0.026103428104839566 1106 30-31 0.07499144036978578 1106 32-33 0.07505911281818811 1106 34-35 0.06704023316535768 1106 36-37 0.11072979560150031 1106 38-39 0.09096582278858989 1106 40-41 0.13874487966125315 1106 42-43 0.11221461426664803 1106 44-45 0.06111627266813002 1106 46-47 0.09154681363674655 1106 48-49 0.09416915564403183 1106 50-51 0.08778843434919992 1106 52-53 0.1341425524322304 1106 54-55 0.10128524058886512 1106 56-57 0.025941201097523958 1106 58-59 0.07065370910945035 1106 60-61 0.0586936060490757 1106 62-63 0.027694746218749344 1106 64-65 0.013800312678085902 1106 66-67 0.021560820303811568 1106 68-69 0.09801899294806304 1106 70-71 0.045549423052264615 1106 72-73 0.062116970357330104 1106 74-75 0.056804721811069214 1106 76-77 0.04321275944224112 1106 78-79 0.015752248194026208 1106 80-81 0.022288758204147996 1106 82-83 0.04824549446041715 1106 84-85 0.0421005027409862 1106 86-87 0.10674770945010437 1106 88-89 0.08830637066136404 1106 90-91 0.05836876942949232 1106 92-93 0.05749837612157194 1106 94-95 0.06067020908189846 1106 96-97 0.023438651916052322 1106 98-99 0.09405190352897819 1106 100 0.15188481544922183 1107 1 0.01870321066541436 1107 2 -0.015994677799128 1107 3 0.03337428553020061 1107 4 0.01192151875500258 1107 5 0.05854895248238989 1107 6 0.07654697269898492 1107 7 0.051248592533362114 1107 8 0.049523158227735564 1107 9 0.05083005314263289 1107 10-11 0.05697467491268782 1107 12-13 0.015198873402013646 1107 14-15 0.07631622484024803 1107 16-17 0.0890812175319482 1107 18-19 0.08336954233433858 1107 20-21 0.03509039741484088 1107 22-23 0.11506663420454544 1107 24-25 0.13797591835407275 1107 26-27 0.11185312759781851 1107 28-29 0.08863908283539956 1107 30-31 0.08505293106664169 1107 32-33 0.03854044071005802 1107 34-35 0.08598569422673563 1107 36-37 0.022161159330948976 1107 38-39 0.03607810776313869 1107 40-41 0.0063145252349414704 1107 42-43 0.006609416355473741 1107 44-45 0.020683351101219216 1107 46-47 0.046521745618484545 1107 48-49 0.03552398055187922 1107 50-51 0.1267124294343347 1107 52-53 0.09478247256563321 1107 54-55 0.05703954841169434 1107 56-57 0.0957389930640602 1107 58-59 0.09641745172251603 1107 60-61 0.12320535565412882 1107 62-63 0.06154297043032386 1107 64-65 0.05937757190808668 1107 66-67 0.03227735239672569 1107 68-69 -0.013014866246834345 1107 70-71 0.05634092150174297 1107 72-73 0.044889793041782866 1107 74-75 0.02440051418717104 1107 76-77 0.0036515503263316873 1107 78-79 0.039629032759002314 1107 80-81 0.09369878394869602 1107 82-83 0.08327763857135295 1107 84-85 0.05122628531660922 1107 86-87 0.01714051487401491 1107 88-89 0.02648746743744823 1107 90-91 0.009951930888739469 1107 92-93 0.10026988232366563 1107 94-95 0.13916729819312934 1107 96-97 0.10308949153925084 1107 98-99 0.11004396440897324 1107 100 0.1396113601218545 1108 1 0.0022756862382919962 1108 2 0.021337185658076407 1108 3 0.01832064130832123 1108 4 0.036012893192484796 1108 5 0.02160885713687577 1108 6 0.005701184268026793 1108 7 0.04827130038798799 1108 8 0.03686184579801477 1108 9 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-0.1909404837737796 2214 58-59 -0.1583220567441117 2214 60-61 -0.2345460722291648 2214 62-63 -0.23103292847986978 2214 64-65 -0.21492586507899603 2214 66-67 -0.20347357021594803 2214 68-69 -0.20557325909123136 2214 70-71 -0.14735156812523797 2214 72-73 -0.14717072028257405 2214 74-75 -0.19236547033230522 2214 76-77 -0.1600408054963225 2214 78-79 -0.13326886944405203 2214 80-81 -0.20365692401635727 2214 82-83 -0.17733505143078787 2214 84-85 -0.13422032803222095 2214 86-87 -0.14268937204221288 2214 88-89 -0.15243264883213925 2214 90-91 -0.124358253659274 2214 92-93 -0.14178604342385626 2214 94-95 -0.23338147029735978 2214 96-97 -0.17451102736666257 2214 98-99 -0.20792412453394604 2214 100 -0.21180629669681394 2215 1 -0.19717651859756558 2215 2 -0.1168748277362539 2215 3 -0.1588571915231327 2215 4 -0.13489431010590636 2215 5 -0.15480617841368627 2215 6 -0.1731065246529795 2215 7 -0.20007763804920842 2215 8 -0.161215490212669 2215 9 -0.20373896619952347 2215 10-11 -0.19078136065420637 2215 12-13 -0.2578806384388628 2215 14-15 -0.31793875018498596 2215 16-17 -0.35728905652618437 2215 18-19 -0.33306644545282893 2215 20-21 -0.30907571019802305 2215 22-23 -0.3217366242487927 2215 24-25 -0.3899405158347804 2215 26-27 -0.3764507474752534 2215 28-29 -0.4050898281056732 2215 30-31 -0.41762180922242464 2215 32-33 -0.38326647650126944 2215 34-35 -0.4068321250870781 2215 36-37 -0.4138710477027203 2215 38-39 -0.3825368060208021 2215 40-41 -0.3772189327507789 2215 42-43 -0.3713252009196282 2215 44-45 -0.3921398207375262 2215 46-47 -0.4624772215157904 2215 48-49 -0.33326263470397777 2215 50-51 -0.3095487959230141 2215 52-53 -0.2604760095534999 2215 54-55 -0.3036445230940572 2215 56-57 -0.2813332422212085 2215 58-59 -0.2920062047771168 2215 60-61 -0.2503935503716974 2215 62-63 -0.29657360501647645 2215 64-65 -0.30655150353871363 2215 66-67 -0.28774109084326227 2215 68-69 -0.33108035746138853 2215 70-71 -0.38317056511639436 2215 72-73 -0.31694684258355466 2215 74-75 -0.35288288844669324 2215 76-77 -0.3336718218861492 2215 78-79 -0.3450949053708996 2215 80-81 -0.3339347468800753 2215 82-83 -0.33633734495593615 2215 84-85 -0.3546628205515745 2215 86-87 -0.27073136660215624 2215 88-89 -0.270122950668366 2215 90-91 -0.274293573433134 2215 92-93 -0.26037115311038406 2215 94-95 -0.2422396796838413 2215 96-97 -0.27111454870376406 2215 98-99 -0.3502835580860193 2215 100 -0.3585014181872985 2216 1 -0.17213336533499302 2216 2 -0.15723824597433378 2216 3 -0.1530491061925403 2216 4 -0.15858123376636257 2216 5 -0.1890112883525248 2216 6 -0.19482145421151387 2216 7 -0.17008537435076931 2216 8 -0.12848401416657396 2216 9 -0.22283098219922692 2216 10-11 -0.25360326895813756 2216 12-13 -0.26299413699165086 2216 14-15 -0.33376798818692066 2216 16-17 -0.3310941914935839 2216 18-19 -0.30922928409842143 2216 20-21 -0.3360530494011371 2216 22-23 -0.36500286910019497 2216 24-25 -0.32665573392928593 2216 26-27 -0.3498906246495821 2216 28-29 -0.3581225843449545 2216 30-31 -0.36716869720170564 2216 32-33 -0.31942993864988267 2216 34-35 -0.38105923008963316 2216 36-37 -0.38210003161612605 2216 38-39 -0.37620172798453666 2216 40-41 -0.35470174053961046 2216 42-43 -0.357637209344432 2216 44-45 -0.3720572751294924 2216 46-47 -0.2710561586554121 2216 48-49 -0.28008103370300574 2216 50-51 -0.28886264633491265 2216 52-53 -0.2715161032292954 2216 54-55 -0.29351978490451813 2216 56-57 -0.23741762561542146 2216 58-59 -0.26597119530407554 2216 60-61 -0.32889025807680383 2216 62-63 -0.29122817218709685 2216 64-65 -0.3327979955051461 2216 66-67 -0.36522493780378085 2216 68-69 -0.31516013666172427 2216 70-71 -0.31072471093605714 2216 72-73 -0.3053229334098191 2216 74-75 -0.39060482069579194 2216 76-77 -0.3202408594783037 2216 78-79 -0.44686065705795386 2216 80-81 -0.3409796676189174 2216 82-83 -0.28613518184268116 2216 84-85 -0.4076362555645403 2216 86-87 -0.38507982916975436 2216 88-89 -0.49638644525240494 2216 90-91 -0.4675178202713468 2216 92-93 -0.41121576753678113 2216 94-95 -0.3635525824596044 2216 96-97 -0.4890739560044608 2216 98-99 -0.5700860579793385 2216 100 -0.5689009620130392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17.0 3 9.0 4 11.0 5 22.0 6 74.0 7 174.0 8 377.0 9 780.0 10 1383.0 11 2252.0 12 3369.0 13 5352.0 14 6343.0 15 8543.0 16 10023.0 17 14198.0 18 16688.0 19 9079.0 20 6245.0 21 6062.0 22 6473.0 23 6880.0 24 8004.0 25 9789.0 26 12551.0 27 16616.0 28 21454.0 29 27735.0 30 36090.0 31 45854.0 32 58765.0 33 76651.0 34 106756.0 35 161683.0 36 295795.0 37 666632.0 38 1198523.0 39 565143.0 40 1868.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8301392533694 19.65446270656229 13.419091577169526 24.09630646289878 2 15.694546957747688 20.232133179020227 39.936677605986034 24.136642257246052 3 18.136371038698876 25.261353025462224 30.12085563752799 26.481420298310915 4 12.250689553264383 16.735063047589644 38.905478261608174 32.1087691375378 5 12.588573495535629 37.76416817138731 36.007035209730965 13.640223123346088 6 30.97657643514513 38.30426793085693 17.428199542271898 13.290956091726041 7 27.171808381486724 32.477170036403166 22.59339716946234 17.757624412647765 8 25.223731223198747 36.091291418649995 20.25643676669538 18.428540591455878 9 25.141882743069292 16.155726726382824 21.164684735768745 37.537705794779136 10-11 23.926496086976897 26.458714091569423 29.008988823281317 20.60580099817236 12-13 24.895094490377573 23.813865539942295 29.18246192516511 22.108578044515024 14-15 21.98470324479421 25.68996414589602 26.518769036921245 25.80656357238852 16-17 21.275811798026364 29.091229937987983 27.023884402392028 22.609073861593625 18-19 21.04991000938714 27.70253745703662 29.73506587840064 21.512486655175593 20-21 22.184571338612656 27.12180156036626 29.33803420211539 21.35559289890569 22-23 21.80923351428041 26.94588399587846 29.38136192402566 21.863520565815474 24-25 21.676579376340623 26.999904370530363 29.377958857068208 21.945557396060796 26-27 21.448678292231662 26.948176426086263 29.53253079659791 22.070614485084164 28-29 21.50230540945639 27.06945370912081 29.494347591456695 21.933893289966104 30-31 21.726912500245295 27.471889231666825 29.028712216201395 21.77248605188648 32-33 21.390560715445766 27.14377890631155 29.592272182898622 21.873388195344063 34-35 21.602890579899675 26.880105604049838 29.580761646071203 21.936242169979288 36-37 21.935509947534797 27.32753745098137 28.934663205499987 21.80228939598385 38-39 21.73621364259285 27.523670554963108 29.000387492117625 21.739728310326417 40-41 21.642947520106844 27.208296966253304 29.158587805263803 21.990167708376045 42-43 21.614342538931535 27.15836477740584 29.045682772533926 22.181609911128696 44-45 21.804207818788417 27.352476947440778 28.906663019222595 21.936652214548204 46-47 21.86174585847663 27.608300825097537 28.561244403257746 21.968708913168083 48-49 21.87726897429987 27.532457224297012 28.403992311078557 22.186281490324557 50-51 21.949348951735704 27.544670694671147 28.220980633302123 22.284999720291026 52-53 21.966087556816802 27.515411085789232 28.230572747324974 22.287928610068995 54-55 22.06004634089407 27.851222943282345 28.007728168568153 22.08100254725544 56-57 21.864323281481248 27.57464788154867 28.187020156326565 22.374008680643524 58-59 21.74582040106459 27.206017814093407 28.554478667870637 22.493683116971365 60-61 22.039134067879363 27.602940956803856 28.112670289312803 22.245254686003978 62-63 21.84550516465779 27.562287966685634 28.1660493055163 22.426157563140272 64-65 21.86504085947685 27.253246161763165 28.354816251706445 22.52689672705354 66-67 21.70753981166653 27.423971146921016 28.179434331801623 22.68905470961083 68-69 22.175587672213656 27.505594559515117 28.08273372708449 22.23608404118674 70-71 22.131351723162307 27.302193256782836 28.12163863887949 22.444816381175368 72-73 21.904254228600717 27.516921725122646 28.172439042249398 22.40638500402724 74-75 22.077119864895913 27.526505404617374 27.9802645173261 22.41611021316061 76-77 22.06543872944743 27.502235109339136 27.846160041883127 22.586166119330308 78-79 22.130442206707567 27.61893269499157 27.930039367207506 22.320585731093356 80-81 22.029410153816503 27.41495016640487 28.065778763967508 22.489860915811114 82-83 21.8612296255205 27.490606307254374 28.1837142407767 22.464449826448433 84-85 21.902047381821493 27.413046388049196 28.0078013908126 22.677104839316716 86-87 22.012133570320714 27.46837406145104 27.904912548054163 22.614579820174086 88-89 21.70239363408091 27.44423918942786 27.988278887381778 22.865088289109455 90-91 21.690951630023456 27.333324741724525 28.058710368275406 22.917013259976613 92-93 21.873057310093618 27.420453415495068 27.90709151651985 22.79939775789147 94-95 21.544716101990907 27.213992081120118 28.102211747807825 23.139080069081146 96-97 21.516626614005105 27.24516768665492 28.12629695204371 23.111908747296262 98-99 21.54300853318517 27.293393279975724 28.001467961491493 23.162130225347614 100 21.66233239794357 27.174092915513537 27.834440404854575 23.32913428168832 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 24.0 1 21.0 2 79.5 3 157.5 4 110.5 5 79.5 6 210.0 7 493.5 8 881.0 9 1053.5 10 895.5 11 750.0 12 816.0 13 1014.5 14 1211.5 15 1446.5 16 1693.5 17 1975.5 18 2332.0 19 2772.5 20 3422.5 21 4253.5 22 5519.0 23 7383.5 24 9901.0 25 13113.0 26 17524.0 27 23058.0 28 29812.0 29 38123.5 30 47304.5 31 57578.5 32 68489.5 33 79698.5 34 90339.5 35 98878.0 36 106117.0 37 114065.0 38 120398.0 39 123169.0 40 125083.0 41 126709.5 42 126845.5 43 125598.5 44 122812.5 45 120020.5 46 119198.5 47 116795.5 48 112999.5 49 110324.0 50 107696.0 51 104555.0 52 99121.5 53 94610.0 54 90967.5 55 85201.0 56 78456.0 57 71453.5 58 65449.5 59 59761.5 60 53016.5 61 45371.5 62 38257.5 63 31490.5 64 25155.5 65 20301.5 66 16126.0 67 12006.0 68 8987.5 69 7031.0 70 5128.0 71 3559.5 72 2557.0 73 1805.5 74 1293.0 75 880.0 76 574.5 77 386.0 78 238.5 79 140.0 80 77.0 81 35.0 82 17.5 83 9.5 84 4.0 85 1.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04366974658952752 2 0.24019825069129122 3 0.0031339120624275284 4 0.0028117341868508666 5 2.3431118223757221E-4 6 2.6360008001726875E-4 7 0.0 8 2.9288897779696524E-4 9 0.0 10-11 6.736446489330201E-4 12-13 0.0 14-15 2.7824452890711697E-4 16-17 1.610889377883309E-4 18-19 5.272001600345375E-4 20-21 1.4644448889848263E-5 22-23 1.4644448889848262E-4 24-25 0.0012740670534167988 26-27 1.7573338667817916E-4 28-29 3.075334266868135E-4 30-31 5.8577795559393054E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.786669333908958E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.489556311274205E-4 70-71 7.322224444924131E-5 72-73 3.8075567113605486E-4 74-75 1.9037783556802743E-4 76-77 1.4644448889848263E-5 78-79 0.0 80-81 0.0 82-83 1.1715559111878611E-4 84-85 0.0 86-87 4.8326681336499264E-4 88-89 0.0024016896179351152 90-91 0.0023138229245960257 92-93 6.297113022634753E-4 94-95 5.125557111446892E-4 96-97 2.3431118223757221E-4 98-99 1.3180004000863438E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3414263.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.661534367074424 #Duplication Level Percentage of deduplicated Percentage of total 1 73.45501695991594 43.82439018788059 2 15.315002979058292 18.27433153133867 3 5.19744936243812 9.302634112746928 4 2.2297555120491563 5.321225404491775 5 1.1754509393613073 3.506460330775727 6 0.6885368211395121 2.4647497930446702 7 0.4362708800364177 1.8220013071852572 8 0.29978224829503686 1.4308375127434543 9 0.20765012342455896 1.114985247751941 >10 0.9407881757308205 9.631510620492968 >50 0.0406970511484241 1.6178861622694034 >100 0.013107468601033912 1.2248145265157855 >500 2.948872828726905E-4 0.10151407808620973 >1k 1.9659151852842932E-4 0.36265918467661945 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4986 0.1460344443295669 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4062 0.11897150278112728 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.9288897779696527E-5 0.0 8 0.0 0.0 0.0 2.9288897779696527E-5 0.0 9 0.0 0.0 0.0 2.9288897779696527E-5 0.0 10-11 0.0 0.0 0.0 2.9288897779696527E-5 0.0 12-13 0.0 0.0 0.0 2.9288897779696527E-5 0.0 14-15 0.0 0.0 0.0 2.9288897779696527E-5 0.0 16-17 0.0 0.0 0.0 2.9288897779696527E-5 0.0 18-19 0.0 0.0 0.0 2.9288897779696527E-5 0.0 20-21 0.0 0.0 0.0 2.9288897779696527E-5 0.0 22-23 0.0 0.0 0.0 2.9288897779696527E-5 0.0 24-25 0.0 0.0 0.0 8.786669333908958E-5 0.0 26-27 0.0 0.0 0.0 8.786669333908958E-5 0.0 28-29 0.0 0.0 0.0 3.9540012002590313E-4 0.0 30-31 0.0 0.0 0.0 0.0028410230846305632 0.0 32-33 0.0 0.0 0.0 0.008728091538349565 0.0 34-35 0.0 0.0 0.0 0.017060782956673227 0.0 36-37 0.0 0.0 0.0 0.03230565425100527 0.0 38-39 0.0 0.0 0.0 0.05913428461720729 0.0 40-41 0.0 0.0 0.0 0.09537929561958174 0.0 42-43 0.0 0.0 0.0 0.13171217331529528 0.0 44-45 0.0 0.0 0.0 0.17154507429568255 0.0 46-47 0.0 0.0 0.0 0.22323997887684693 0.0 48-49 0.0 0.0 0.0 0.2814663076628836 0.0 50-51 0.0 0.0 0.0 0.3442031267069936 0.0 52-53 0.0 0.0 0.0 0.4094880798579371 0.0 54-55 0.0 0.0 0.0 0.4815094794982109 0.0 56-57 1.4644448889848263E-5 0.0 0.0 0.5661104607348644 0.0 58-59 2.9288897779696527E-5 0.0 0.0 0.6629981345901004 0.0 60-61 2.9288897779696527E-5 0.0 0.0 0.7695218558148567 1.4644448889848263E-5 62-63 2.9288897779696527E-5 0.0 0.0 0.8801313782798805 2.9288897779696527E-5 64-65 2.9288897779696527E-5 0.0 0.0 1.0010505927633577 2.9288897779696527E-5 66-67 2.9288897779696527E-5 0.0 0.0 1.1343443665587567 2.9288897779696527E-5 68-69 2.9288897779696527E-5 0.0 0.0 1.2865880572176192 2.9288897779696527E-5 70-71 2.9288897779696527E-5 0.0 0.0 1.453373685624101 2.9288897779696527E-5 72-73 2.9288897779696527E-5 0.0 0.0 1.6334271847247854 2.9288897779696527E-5 74-75 2.9288897779696527E-5 0.0 0.0 1.8151647954478023 2.9288897779696527E-5 76-77 2.9288897779696527E-5 0.0 0.0 2.0130259443985423 2.9288897779696527E-5 78-79 2.9288897779696527E-5 0.0 0.0 2.2400881244356396 2.9288897779696527E-5 80-81 2.9288897779696527E-5 0.0 0.0 2.4975521803680616 2.9288897779696527E-5 82-83 2.9288897779696527E-5 0.0 0.0 2.7661753063545484 2.9288897779696527E-5 84-85 2.9288897779696527E-5 0.0 0.0 3.029921830860716 2.9288897779696527E-5 86-87 2.9288897779696527E-5 2.9288897779696527E-5 0.0 3.3147710062171543 2.9288897779696527E-5 88 2.9288897779696527E-5 2.9288897779696527E-5 0.0 3.5470905434057074 2.9288897779696527E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4425 0.0 49.843113 1 GTATCAA 7845 0.0 42.231354 1 TCAACGC 9085 0.0 35.694344 4 ATCAACG 9220 0.0 35.27469 3 CAACGCA 9340 0.0 34.72033 5 AACGCAG 9690 0.0 33.563244 6 TATCAAC 9865 0.0 33.048676 2 ACGCAGA 11355 0.0 28.434868 7 CGCAGAG 11600 0.0 27.75368 8 GTACATG 8635 0.0 26.961077 1 TACATGG 8770 0.0 25.302671 2 GCAGAGT 13150 0.0 24.160667 9 ACATGGG 8950 0.0 23.420694 3 GAGTACT 9110 0.0 20.919788 12-13 GTGGTAT 2190 0.0 20.87374 1 TATACCG 160 5.4184282E-5 20.56173 5 CAGAGTA 12740 0.0 20.530052 10-11 AGAGTAC 11905 0.0 20.193453 10-11 GAGTACA 6370 0.0 19.901484 1 CATGGGG 6600 0.0 19.297482 4 >>END_MODULE