FastQCFastQC Report
Wed 25 May 2016
SRR1033033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152308
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22931.5055020090868503No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2520.16545421120361373No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1980.12999973737426793No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1840.12080783675184495No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAG200.002158345270.5083853
GTACATA250.005218079356.425251
GTATAAC250.005218079356.425251
TACATGA602.5340341E-854.839852
TACAAGA602.5340341E-854.839852
CTAACAA352.9276783E-453.7206738
CAGGCTA555.257732E-542.7323534
TCAACAC2050.041.2731973
GTACAAG802.4312612E-741.1434141
GAGAACA2600.039.787041
CGTATCA250.001704564237.5797690-91
TTAGACC651.4067489E-436.1581424
AGAACAA2650.035.4759142
GCTAACA550.002708058834.1858837
GCCGTAT1001.4064353E-632.88229494
ACTATTA600.004147330831.3370573
TACAGTG600.004147330831.3370577
TCTATTC600.004147330831.3370573
ATACGGC300.004160489431.3267682-83
GGCTAAC600.00415401231.326766
Produced by FastQC (version 0.11.5)