##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033033_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 152308 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.308736244977283 33.0 30.0 34.0 15.0 34.0 2 29.28620295716574 33.0 30.0 34.0 16.0 34.0 3 29.413668356225543 33.0 31.0 34.0 16.0 34.0 4 32.38516033300943 37.0 35.0 37.0 17.0 37.0 5 32.796819602384645 37.0 35.0 37.0 19.0 37.0 6 32.61519421172886 37.0 35.0 37.0 17.0 37.0 7 32.75904745646979 37.0 35.0 37.0 18.0 37.0 8 32.406840087191746 37.0 35.0 37.0 13.0 37.0 9 33.6949996060614 39.0 35.0 39.0 12.0 39.0 10-11 33.94476324290254 39.0 35.0 39.0 13.0 39.0 12-13 33.91692819812485 39.0 35.0 39.0 14.0 39.0 14-15 35.12702221813693 40.0 36.0 41.0 12.5 41.0 16-17 35.24936969824303 40.0 36.0 41.0 16.0 41.0 18-19 35.24366415421383 40.0 36.0 41.0 17.5 41.0 20-21 35.27124970454605 40.0 35.0 41.0 18.5 41.0 22-23 35.2118568952386 40.0 35.0 41.0 21.5 41.0 24-25 35.15124615909867 40.0 35.0 41.0 23.0 41.0 26-27 34.82565262494419 40.0 35.0 41.0 19.5 41.0 28-29 34.60840533655488 40.0 35.0 41.0 13.0 41.0 30-31 34.46138088609922 40.0 35.0 41.0 2.0 41.0 32-33 34.203223074296815 40.0 35.0 41.0 2.0 41.0 34-35 33.955192767287336 40.0 35.0 41.0 2.0 41.0 36-37 33.67890393150721 40.0 35.0 41.0 2.0 41.0 38-39 33.43397917377945 40.0 34.5 41.0 2.0 41.0 40-41 33.26053457467763 40.0 34.0 41.0 2.0 41.0 42-43 33.04706253118681 40.0 34.0 41.0 2.0 41.0 44-45 32.862755075242276 40.0 33.0 41.0 2.0 41.0 46-47 32.68788244872233 40.0 33.0 41.0 2.0 41.0 48-49 32.47792630721958 39.5 33.0 41.0 2.0 41.0 50-51 32.30546983743467 39.0 33.0 41.0 2.0 41.0 52-53 32.13584644273446 39.0 33.0 41.0 2.0 41.0 54-55 31.94020668645114 39.0 32.5 41.0 2.0 41.0 56-57 31.74020734301547 38.5 32.0 41.0 2.0 41.0 58-59 31.53736507603015 38.0 32.0 41.0 2.0 41.0 60-61 31.293753446962732 37.5 31.0 40.5 2.0 41.0 62-63 31.013778002468683 37.0 31.0 40.0 2.0 41.0 64-65 30.721938440528405 36.5 31.0 40.0 2.0 41.0 66-67 30.414965070777633 36.0 30.5 39.5 2.0 41.0 68-69 30.06421855713423 35.0 30.0 39.0 2.0 41.0 70-71 29.72036925177929 35.0 30.0 39.0 2.0 41.0 72-73 29.36373663891588 35.0 29.5 37.5 2.0 40.0 74-75 29.03671179452163 35.0 29.0 37.0 2.0 39.5 76-77 28.702215248050003 35.0 29.0 36.5 2.0 39.0 78-79 28.35187252146965 35.0 28.5 36.0 2.0 39.0 80-81 28.048871365916433 35.0 28.0 36.0 2.0 37.5 82-83 27.856356855844737 35.0 28.0 35.0 2.0 37.0 84-85 27.669216324815505 35.0 28.0 35.0 2.0 37.0 86-87 27.548122882580035 35.0 28.0 35.0 2.0 36.0 88-89 27.33603618982588 35.0 27.0 35.0 2.0 36.0 90-91 27.125636867400267 35.0 27.0 35.0 2.0 36.0 92-93 26.894765869159862 34.5 25.5 35.0 2.0 35.0 94-95 26.684369829555898 34.0 25.0 35.0 2.0 35.0 96-97 26.510826745804554 34.0 25.0 35.0 2.0 35.0 98-99 26.312117551277673 34.0 24.0 35.0 2.0 35.0 100 26.162690075373586 34.0 24.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03112004041298988 1101 2 0.04489346970055763 1101 3 -0.16491706067119694 1101 4 -0.4190076633563038 1101 5 -0.6026969563323661 1101 6 -0.8135211173094419 1101 7 -0.08287684740563606 1101 8 -0.2627564128289137 1101 9 -0.7868094454263073 1101 10-11 -0.29231091538101595 1101 12-13 -0.5095599188334461 1101 14-15 -0.2274207101716641 1101 16-17 -0.15037510873550275 1101 18-19 -0.1532704331222874 1101 20-21 -0.27000751181506644 1101 22-23 -0.19117020065247203 1101 24-25 -0.06655057521279417 1101 26-27 -0.1255287894990076 1101 28-29 0.08222481482754773 1101 30-31 0.372149036939625 1101 32-33 0.5125276617827552 1101 34-35 0.5642107111756545 1101 36-37 0.34937118094637754 1101 38-39 0.3408375079749675 1101 40-41 0.02101382480314129 1101 42-43 -0.18511509893674116 1101 44-45 0.12532391239565754 1101 46-47 0.09337309059272059 1101 48-49 0.22319979381628485 1101 50-51 0.41910412239903394 1101 52-53 0.4047252676913402 1101 54-55 0.4352870945399019 1101 56-57 0.33110407685151344 1101 58-59 0.3023524128526702 1101 60-61 0.7947006280046587 1101 62-63 0.5820212191806071 1101 64-65 0.45878173869180117 1101 66-67 0.5404204672056707 1101 68-69 0.4551651381969357 1101 70-71 0.2718596441054828 1101 72-73 0.27131938326434124 1101 74-75 0.24218612864220646 1101 76-77 0.08452967174137171 1101 78-79 0.3926315087241754 1101 80-81 0.45613954285899183 1101 82-83 0.41034270620407653 1101 84-85 0.1592567020923319 1101 86-87 0.55224794940316 1101 88-89 0.7850134605749481 1101 90-91 0.9161172244250722 1101 92-93 0.785516931408889 1101 94-95 0.42157115884277374 1101 96-97 0.31204457904229344 1101 98-99 0.20295650475084415 1101 100 0.47629988118990596 1102 1 0.4795976045155541 1102 2 0.704508854315943 1102 3 0.6853233239441856 1102 4 0.38940579818216037 1102 5 -0.06479631530672236 1102 6 0.6387224724341465 1102 7 0.7911616141328253 1102 8 -0.21620192564942187 1102 9 0.32561042636856996 1102 10-11 0.5364951743625781 1102 12-13 0.6224512991152693 1102 14-15 0.5788693539309051 1102 16-17 0.6554742502388606 1102 18-19 0.7149186694418148 1102 20-21 0.8020277445951791 1102 22-23 0.5214900557577877 1102 24-25 0.21982762991540739 1102 26-27 0.3181010181933033 1102 28-29 0.014877058417297917 1102 30-31 0.11305448565757104 1102 32-33 0.14281612332122506 1102 34-35 0.4182171214320647 1102 36-37 0.6542990655617587 1102 38-39 0.7216468028467631 1102 40-41 0.41997215813647415 1102 42-43 0.581952208755574 1102 44-45 0.5569344893187349 1102 46-47 0.33367757777221385 1102 48-49 0.9567735117650074 1102 50-51 0.9014759172708295 1102 52-53 0.9973134087169839 1102 54-55 0.8072822868475882 1102 56-57 0.9970095255694638 1102 58-59 0.8256697205449797 1102 60-61 0.7477454997995316 1102 62-63 0.8223257063600968 1102 64-65 0.6925958412559048 1102 66-67 0.48300860823131586 1102 68-69 0.42259302281231825 1102 70-71 0.3417715030798405 1102 72-73 0.572120665315623 1102 74-75 0.5713928594114357 1102 76-77 0.40291909481829435 1102 78-79 0.7070545856472528 1102 80-81 0.2258110172179677 1102 82-83 0.21106386005023126 1102 84-85 0.4128624713230984 1102 86-87 0.2785300006852154 1102 88-89 -0.03221410352761822 1102 90-91 0.11551626288660799 1102 92-93 0.2518630852550423 1102 94-95 0.0791432742273912 1102 96-97 0.06340675852947442 1102 98-99 0.2749516970585333 1102 100 0.38295052221554826 1103 1 0.20741423233218015 1103 2 0.455247005054094 1103 3 0.050202888823751124 1103 4 0.4763521851285475 1103 5 0.3052891547787482 1103 6 0.4206960544077276 1103 7 0.5427544657256789 1103 8 0.29358828414078886 1103 9 0.2124014131595544 1103 10-11 0.6305899684573788 1103 12-13 0.3438965205604916 1103 14-15 0.2546657797273326 1103 16-17 0.7121564569210648 1103 18-19 1.2083141628373113 1103 20-21 1.0158195083869472 1103 22-23 0.7100292942970228 1103 24-25 0.6818711419589221 1103 26-27 0.41242891252119307 1103 28-29 0.5000751936154302 1103 30-31 0.3567607793638672 1103 32-33 0.04440897491407014 1103 34-35 -0.15012009690515526 1103 36-37 -0.17375066248796145 1103 38-39 -0.09847751727755849 1103 40-41 -0.17043188226756456 1103 42-43 -0.33079060398724636 1103 44-45 -0.4614027290184879 1103 46-47 -0.6580473639527327 1103 48-49 -0.5642055092140126 1103 50-51 -0.496931231136319 1103 52-53 -0.4937122323086598 1103 54-55 -0.667853688288389 1103 56-57 -0.4254457968858567 1103 58-59 -0.3512145063392502 1103 60-61 -0.08314028108624782 1103 62-63 -0.09616691213252082 1103 64-65 -0.16653391787385274 1103 66-67 -0.06003723481452994 1103 68-69 -0.007981957762662972 1103 70-71 0.043403204711538024 1103 72-73 0.10118049437545196 1103 74-75 0.15830039631897463 1103 76-77 -0.04591540401619909 1103 78-79 0.024575953168621822 1103 80-81 0.11104800245495383 1103 82-83 0.30822780721417686 1103 84-85 0.43403574249637344 1103 86-87 0.439874212029423 1103 88-89 0.4657268444133287 1103 90-91 0.3366506840210306 1103 92-93 0.348016931408889 1103 94-95 0.315273810357926 1103 96-97 0.5192731143958298 1103 98-99 0.852340974447813 1103 100 0.5702077094727329 1104 1 0.19371286219516648 1104 2 -0.07680620026643936 1104 3 0.047902658800747844 1104 4 -0.22691814190415727 1104 5 0.11166979284255518 1104 6 -0.19656567176488693 1104 7 -0.3083306427851724 1104 8 -0.13440451513914553 1104 9 0.6623964126595041 1104 10-11 0.6386657760381311 1104 12-13 0.5752946603744746 1104 14-15 0.4363839515445136 1104 16-17 0.7042556668420588 1104 18-19 0.676711002521273 1104 20-21 0.6086537918152928 1104 22-23 0.46342963433102824 1104 24-25 -0.08480552570784283 1104 26-27 -0.07011934230428807 1104 28-29 -0.211220935997531 1104 30-31 0.030178121098039412 1104 32-33 -0.05007547353076802 1104 34-35 -0.07718780367583378 1104 36-37 0.18948566114439558 1104 38-39 0.29063639411357656 1104 40-41 0.39224938585925173 1104 42-43 0.5835008251556744 1104 44-45 0.6272061318675952 1104 46-47 0.5349469354772154 1104 48-49 0.7941053218690897 1104 50-51 0.7155150134881438 1104 52-53 0.8156937082854014 1104 54-55 0.8864267397544197 1104 56-57 0.7387206197558065 1104 58-59 1.0234229574071207 1104 60-61 1.0165446874105974 1104 62-63 1.0615488594446383 1104 64-65 0.6963023657545087 1104 66-67 0.41838560746969833 1104 68-69 0.9072845688900024 1104 70-71 0.6544143058216534 1104 72-73 0.735118888214835 1104 74-75 0.5399635626356023 1104 76-77 0.8730764951737164 1104 78-79 0.8458080763809406 1104 80-81 0.8304949471494218 1104 82-83 0.9869206765011072 1104 84-85 0.7426416030824292 1104 86-87 0.9932545500632273 1104 88-89 0.8599412658554719 1104 90-91 1.0445464735999899 1104 92-93 1.2333304627620265 1104 94-95 1.1489071736942584 1104 96-97 1.0598271698013733 1104 98-99 1.2844591862689931 1104 100 1.30628131683347 1105 1 0.8519879314082495 1105 2 0.8993563448980311 1105 3 0.8131881920934134 1105 4 1.2289731818253742 1105 5 1.0417293864796449 1105 6 0.647083148093401 1105 7 0.8402076707618207 1105 8 1.382558555575514 1105 9 1.0781441485705372 1105 10-11 0.813892184934943 1105 12-13 0.9353082683703491 1105 14-15 1.0937557313765254 1105 16-17 1.172171297267667 1105 18-19 0.7237289744606485 1105 20-21 0.8467233333666044 1105 22-23 0.9587606840236802 1105 24-25 1.2170690930336505 1105 26-27 1.1835524703746145 1105 28-29 0.986611169487901 1105 30-31 1.5136256364656973 1105 32-33 1.7830154184968166 1105 34-35 1.554880142455282 1105 36-37 1.269209747297083 1105 38-39 1.2869645814346882 1105 40-41 1.1768441960511709 1105 42-43 1.2200734871612084 1105 44-45 1.475430053785857 1105 46-47 1.514112624557967 1105 48-49 1.3367710813360318 1105 50-51 1.1350401413563844 1105 52-53 1.5098422700610072 1105 54-55 1.2018624302428975 1105 56-57 1.223962982854676 1105 58-59 0.9980721995825306 1105 60-61 0.8010691114169823 1105 62-63 0.8874368984855074 1105 64-65 0.8059875048845342 1105 66-67 0.7810035043304744 1105 68-69 1.0536174486234735 1105 70-71 0.8651036211986884 1105 72-73 1.0493110894254798 1105 74-75 0.9423613853562642 1105 76-77 0.5136494387239949 1105 78-79 0.6271299289453438 1105 80-81 0.562194538909548 1105 82-83 0.6373124929339369 1105 84-85 0.563062981713184 1105 86-87 0.21081824956113948 1105 88-89 0.2710669755828441 1105 90-91 0.16773402932238568 1105 92-93 0.3104971841735029 1105 94-95 0.11636529391386574 1105 96-97 0.36464627730453714 1105 98-99 0.14883673776822093 1105 100 -0.13485236999492756 1106 1 0.8126648517337429 1106 2 0.713918626933264 1106 3 0.5755507066243979 1106 4 0.8715780284675958 1106 5 0.24449250907644426 1106 6 0.39992227891697496 1106 7 0.3931451651729816 1106 8 0.5808953167308388 1106 9 0.8758281331900477 1106 10-11 0.5124748550592386 1106 12-13 0.61912522267599 1106 14-15 0.7144523244195327 1106 16-17 0.42637410510098306 1106 18-19 0.8269772083871487 1106 20-21 0.5530074252918453 1106 22-23 0.5940106169575969 1106 24-25 0.9104541417683336 1106 26-27 1.0012892136456486 1106 28-29 1.0583942330665437 1106 30-31 1.2016006878830225 1106 32-33 1.3436990454305544 1106 34-35 1.3914512772133918 1106 36-37 1.2380799073614668 1106 38-39 1.2310202909938326 1106 40-41 1.4478023468157204 1106 42-43 1.86215298282427 1106 44-45 1.5911749344082295 1106 46-47 1.177787398768828 1106 48-49 1.2852084416150333 1106 50-51 1.4533022356065786 1106 52-53 1.393080220521533 1106 54-55 1.3586852863637944 1106 56-57 1.6267506963483775 1106 58-59 1.59392080907908 1106 60-61 1.5500072317782418 1106 62-63 1.4085738921365838 1106 64-65 1.4184908581886617 1106 66-67 1.241825734501269 1106 68-69 1.2507134872535381 1106 70-71 0.9702185277532784 1106 72-73 1.0207297606228316 1106 74-75 0.7318185971956623 1106 76-77 0.8796849390369701 1106 78-79 0.727930250862542 1106 80-81 0.6152885208464127 1106 82-83 0.3180667628078524 1106 84-85 0.4596694379413897 1106 86-87 0.784166188396874 1106 88-89 0.7933664479963305 1106 90-91 0.9509540954942537 1106 92-93 1.1554068685158079 1106 94-95 1.182626583371075 1106 96-97 1.4186680067152508 1106 98-99 0.9573295393420374 1106 100 1.076830541567265 1107 1 -0.04645186224187725 1107 2 -0.03733366894250878 1107 3 0.18182888328160374 1107 4 0.10279359033101088 1107 5 -0.5123116318485899 1107 6 -0.18022465713244884 1107 7 -0.08748599194840523 1107 8 0.07596961961946391 1107 9 0.15617543657959487 1107 10-11 -0.006935731015225599 1107 12-13 0.3049450590426588 1107 14-15 0.37391133260120313 1107 16-17 0.07865328359488899 1107 18-19 0.22297403590425802 1107 20-21 0.29355259142976564 1107 22-23 0.5985606253062414 1107 24-25 0.5841684513358771 1107 26-27 0.6123261146307897 1107 28-29 0.6758595862139813 1107 30-31 0.8245135280900655 1107 32-33 0.32936025765296506 1107 34-35 0.3327394722375985 1107 36-37 0.4329580172295664 1107 38-39 0.3028812026652332 1107 40-41 0.5359013616762951 1107 42-43 0.6307495765794897 1107 44-45 0.663787223605091 1107 46-47 0.41006741212665077 1107 48-49 0.7196507525183549 1107 50-51 0.5335952728415094 1107 52-53 0.40894263494797656 1107 54-55 0.3620247420325242 1107 56-57 0.4069936417482758 1107 58-59 0.8312517491358591 1107 60-61 0.8547116899515608 1107 62-63 0.8751167206555373 1107 64-65 0.9929815911991753 1107 66-67 0.8049623182381218 1107 68-69 0.6941723284624217 1107 70-71 0.6760124833385177 1107 72-73 0.399361418662572 1107 74-75 0.5794141891141784 1107 76-77 0.7282197528623158 1107 78-79 0.7091875559218153 1107 80-81 0.508384196251324 1107 82-83 0.565624785850094 1107 84-85 0.311773295012685 1107 86-87 0.4641211057453454 1107 88-89 0.635723268834532 1107 90-91 0.9581756683778728 1107 92-93 0.5640936275740849 1107 94-95 0.5819002517631269 1107 96-97 0.6756599920216466 1107 98-99 1.0404611138953825 1107 100 1.0428098369421193 1108 1 -0.15260840786287133 1108 2 0.20293944671205466 1108 3 -0.1521656238895872 1108 4 0.2155110148046191 1108 5 -0.041454140240411164 1108 6 0.2575995723457325 1108 7 -0.023250410624022777 1108 8 0.279089851538906 1108 9 0.4414592327346156 1108 10-11 0.3293665271477195 1108 12-13 -0.11489397055758133 1108 14-15 0.07151966339156246 1108 16-17 -0.00795413172400572 1108 18-19 0.04554422205012543 1108 20-21 0.08972668358723013 1108 22-23 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-1.5099953347760398 2215 70-71 -1.4214821351737967 2215 72-73 -1.4220927788978202 2215 74-75 -1.457912407393831 2215 76-77 -1.3496623327631312 2215 78-79 -1.1653639867713181 2215 80-81 -1.3913407499338 2215 82-83 -1.1880525640661936 2215 84-85 -1.0692061357455067 2215 86-87 -1.0840133118581008 2215 88-89 -1.090971899785412 2215 90-91 -0.9754227530524062 2215 92-93 -1.2004064920145332 2215 94-95 -1.3970521519680368 2215 96-97 -1.6388281687054516 2215 98-99 -1.6550812204743863 2215 100 -1.3053724161073923 2216 1 -0.16009024386794835 2216 2 -0.09905859272976514 2216 3 -0.24488849322415263 2216 4 -0.6993344470798242 2216 5 -0.4708163821963538 2216 6 -0.8379776390485709 2216 7 -0.9069431796241432 2216 8 -0.6001484139437387 2216 9 -0.5185367308220705 2216 10-11 -0.8457790731290586 2216 12-13 -0.5787750108066945 2216 14-15 -0.8369611923344209 2216 16-17 -0.6793083584567938 2216 18-19 -0.39266215553031714 2216 20-21 -0.3976378072442799 2216 22-23 -0.10403948716305678 2216 24-25 -0.3321233176877101 2216 26-27 -0.31578972874092415 2216 28-29 -0.32547968628728086 2216 30-31 -0.9533892155687411 2216 32-33 -0.3988784140254893 2216 34-35 -0.3077583523694969 2216 36-37 -0.6547310347726807 2216 38-39 -0.509679841523365 2216 40-41 -0.6526030926996498 2216 42-43 -0.9767991847784288 2216 44-45 -0.8110699699900792 2216 46-47 -0.6480392561185369 2216 48-49 -0.9440068672762401 2216 50-51 -0.9151350080357474 2216 52-53 -1.1099329680946113 2216 54-55 -0.8259395637655658 2216 56-57 -0.9101253685219497 2216 58-59 -1.0097817844717447 2216 60-61 -0.9474607432327993 2216 62-63 -0.8522562334392347 2216 64-65 -0.8683852066816655 2216 66-67 -0.7437472446114981 2216 68-69 -0.996470522338182 2216 70-71 -0.9843154534419014 2216 72-73 -0.8861847973376626 2216 74-75 -1.1375257582028375 2216 76-77 -1.1460218193400067 2216 78-79 -1.2724325938399232 2216 80-81 -1.0447575446549422 2216 82-83 -1.4892705881103048 2216 84-85 -1.2694117761685355 2216 86-87 -1.2996193861598258 2216 88-89 -1.728869621922268 2216 90-91 -1.7511504037800556 2216 92-93 -1.6505895345888781 2216 94-95 -1.9616594013578954 2216 96-97 -2.0054895625407596 2216 98-99 -1.7700873218935271 2216 100 -1.5691119081077538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6922.0 3 853.0 4 673.0 5 714.0 6 1665.0 7 3092.0 8 3457.0 9 2023.0 10 1195.0 11 1139.0 12 1122.0 13 732.0 14 674.0 15 571.0 16 535.0 17 574.0 18 477.0 19 458.0 20 418.0 21 413.0 22 485.0 23 773.0 24 1224.0 25 1169.0 26 1150.0 27 1015.0 28 1113.0 29 1325.0 30 1554.0 31 1940.0 32 2522.0 33 3406.0 34 4985.0 35 8676.0 36 14346.0 37 27322.0 38 38957.0 39 12630.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.768480803090426 20.782198512600846 13.284760728457679 21.16455995585105 2 16.358596408255494 25.756381455352802 34.97746948856396 22.907552647827746 3 19.015523278348212 30.498019353185786 27.480078568941618 23.00637879952438 4 13.085843818785111 22.086796543907486 33.029994415059626 31.797365222247777 5 13.106792926471844 42.24008201192064 30.053951752281943 14.59917330932557 6 28.365928028809506 39.946245038509055 15.849827826406958 15.837999106274477 7 25.30295462909416 38.75322012512486 19.283292150780714 16.66053309500026 8 22.565952916124452 39.99040445864059 18.727736372359583 18.715906252875374 9 23.49713129029502 23.788931315268897 18.445179056118928 34.268758338317156 10-11 21.866589552067143 33.72920288259631 23.842213013745898 20.561994551590654 12-13 22.86511426149023 32.720455337132194 23.812199722643953 20.602230678733626 14-15 21.329775522745123 33.81617905601909 22.181875540161744 22.672169881074048 16-17 20.104938758345163 36.426956841717924 22.905495978552278 20.56260842138464 18-19 20.53549423791638 34.74469056937338 24.912508995553978 19.807306197156255 20-21 22.714920826857423 32.972434800038116 24.126203862216716 20.186440510887746 22-23 22.23010570435628 33.37734944191516 23.619897908906363 20.772646944822196 24-25 21.978768884233617 33.53767652920838 23.49870137545846 20.984853211099548 26-27 21.43162519649822 32.800239415405464 24.424975499385017 21.3431598887113 28-29 21.31680699598887 33.35775271597192 25.08516670335049 20.24027358468872 30-31 21.813905050378953 33.606226161574014 24.050815886042056 20.529052902004974 32-33 20.652598863931296 33.77159696829324 24.58218757289336 20.993616594882106 34-35 20.849053196344784 33.49816615279543 24.64874520195304 21.004035448906752 36-37 21.02453941382629 33.88219373359742 24.2838702048619 20.809396647714394 38-39 20.51987988619414 33.048380631977345 24.79285625110882 21.638883230719696 40-41 20.47746247030645 33.3592895232275 25.41998100926866 20.743266997197388 42-43 20.02345767433914 34.03366866199266 24.777414924863166 21.165458738805036 44-45 20.292121395296885 33.44021235635394 25.1151468162973 21.152519432051882 46-47 20.730757731569245 33.25240086868242 24.6588844461821 21.35795695356623 48-49 20.111834626681297 32.54078809900847 24.645013765778078 22.70236350853215 50-51 19.759454123150817 32.286434224402164 24.79771083901955 23.156400813427467 52-53 20.556086695118225 31.616383629512086 24.51006482092931 23.31746485444038 54-55 20.585066193620445 31.320258861404028 24.580992740054533 23.513682204920993 56-57 21.059374650959573 31.020085018035125 24.635848176448558 23.284692154556744 58-59 21.032662433438343 30.984143461107628 24.331098460335657 23.652095645118372 60-61 21.669803790170786 31.01547538097162 23.79220745091469 23.522513377942904 62-63 21.77968022654179 30.852192652503422 24.15810619474841 23.21002092620638 64-65 21.76206822922655 31.289851025737892 24.14926991114104 22.79881083389452 66-67 22.570111725420244 31.4667533926618 23.682109806936108 22.28102507498185 68-69 22.706218586774416 31.663545875628262 23.764659505272494 21.865576032324824 70-71 22.889512272034477 31.820899248436437 23.732393440899774 21.557195038629313 72-73 22.945989534918095 31.874156240453814 23.96720546838304 21.212648756245052 74-75 23.000486164034374 31.48372007463275 23.857186030010773 21.658607731322103 76-77 23.32612894325393 30.768220099591385 24.16175060109577 21.743900356058916 78-79 23.962647765008064 29.91496224376176 24.835032008224587 21.287357983005588 80-81 23.805801518694658 29.494337738774007 24.971425943929162 21.72843479860217 82-83 24.03531591186788 29.46145862073496 24.933979740648773 21.569245726748388 84-85 24.111350864920077 29.215151127007115 24.772808633764562 21.900689374308243 86-87 24.367639428034707 28.994331579604193 24.822163983526767 21.81586500883433 88-89 24.25291395578735 28.95859538956198 24.53010512902198 22.25838552562869 90-91 24.69301227294074 28.448459888798027 24.627657303499937 22.23087053476129 92-93 24.21810084635267 28.757598946410802 24.71533714525934 22.308963061977188 94-95 23.231381100795105 29.766592553425575 24.749990968475053 22.25203537730427 96-97 23.396030596527183 29.772825055258327 24.63256907701352 22.19857527120097 98-99 22.652762952623366 30.891534608752767 24.02331882748994 22.43238361113392 100 22.238645172309592 31.08207533535664 24.034658993864387 22.64462049846938 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1692.0 1 1076.0 2 426.5 3 336.5 4 235.0 5 173.5 6 137.0 7 144.0 8 166.5 9 156.0 10 125.5 11 94.0 12 89.5 13 90.0 14 92.5 15 96.5 16 111.5 17 131.0 18 143.5 19 179.0 20 230.0 21 293.5 22 394.5 23 540.5 24 667.5 25 781.0 26 1010.5 27 1294.5 28 1604.0 29 1906.0 30 2068.5 31 2287.0 32 2554.5 33 3091.5 34 3755.0 35 3982.0 36 4192.5 37 4511.0 38 4624.5 39 4694.0 40 4737.5 41 4620.5 42 4586.5 43 4986.5 44 5275.5 45 5102.5 46 5095.5 47 5034.5 48 5078.5 49 5234.0 50 5193.0 51 5046.5 52 4634.5 53 4386.5 54 4001.5 55 3527.5 56 3193.5 57 2936.0 58 2703.5 59 2511.0 60 2357.0 61 2205.0 62 1938.5 63 1562.5 64 1450.0 65 1207.0 66 854.5 67 604.0 68 525.5 69 526.5 70 392.5 71 278.0 72 292.5 73 258.0 74 194.0 75 142.5 76 79.0 77 51.5 78 36.0 79 34.5 80 30.5 81 23.5 82 18.5 83 9.0 84 4.0 85 4.5 86 3.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.5 92 1.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06303017569661476 2 0.04595950311211493 3 0.05580796806471098 4 0.07419176930955694 5 0.0886361845733645 6 0.08929274890353757 7 0.09191900622422985 8 0.10111090684665283 9 0.09782808519578749 10-11 0.1007826246815663 12-13 0.10308059983717205 14-15 0.10143918901173937 16-17 0.08141397694146073 18-19 0.09815636736087402 20-21 0.09290385271948946 22-23 0.0597473540457494 24-25 0.020681776400451716 26-27 0.17792893347690208 28-29 0.06992410116343199 30-31 0.07320692281429735 32-33 0.0764897444651627 34-35 0.021338340730624787 36-37 0.05482312156945137 38-39 0.07813115529059537 40-41 0.08436851642723954 42-43 0.07813115529059537 44-45 0.07287864064921082 46-47 0.08108569477637419 48-49 0.07813115529059537 50-51 0.07386348714447041 52-53 0.0784594374556819 54-55 0.06762612600782625 56-57 0.0689392546681724 58-59 0.0653281508522205 60-61 0.06467158652204744 62-63 0.06992410116343199 64-65 0.06598471518239357 66-67 0.06861097250308586 68-69 0.06762612600782625 70-71 0.060403918375922475 72-73 0.05744937889014366 74-75 0.06303017569661476 76-77 0.056464532394884054 78-79 0.05350999290910524 80-81 0.04727263177246106 82-83 0.05383827507419176 84-85 0.04497465661685532 86-87 0.04005042414055729 88-89 0.043661527956509176 90-91 0.0410352706358169 92-93 0.043661527956509176 94-95 0.041691834965989966 96-97 0.04497465661685532 98-99 0.04628778527720146 100 0.05383827507419176 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 152308.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.9127294692334 #Duplication Level Percentage of deduplicated Percentage of total 1 78.29069236758033 38.29411455734433 2 8.229751134258638 8.050791816582189 3 3.6497624097291204 5.355595241221735 4 2.2228784665360144 4.3490821230664185 5 1.4752073881178018 3.6078209943010218 6 1.1141238690971569 3.26969036426189 7 0.8214985637198314 2.8127215904614333 8 0.646997234825096 2.5317120571473595 9 0.5678004778651776 2.499540404968879 >10 2.884641198421434 24.163537043359508 >50 0.08590834653279283 2.8048428184993566 >100 0.009396225402024215 0.755048979699031 >500 0.0 0.0 >1k 0.001342317914574888 1.5055020090868503 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2293 1.5055020090868503 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 252 0.16545421120361373 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 198 0.12999973737426793 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 184 0.12080783675184495 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.565643301730703E-4 0.0 16-17 0.0 0.0 0.0 9.848464952596054E-4 0.0 18-19 0.0 0.0 0.0 0.0019696929905192112 0.0 20-21 0.0 0.0 0.0 0.002297975155605746 0.0 22-23 0.0 0.0 0.0 0.006893925466817239 0.0 24-25 0.0 0.0 0.0 0.016085826089240224 0.0 26-27 0.0 0.0 0.0 0.04005042414055729 0.0 28-29 0.0 0.0 0.0 0.08305538776689339 0.0 30-31 0.0 0.0 0.0 0.1782572156419886 0.0 32-33 0.0 0.0 0.0 0.32138823961971796 0.0 34-35 0.0 0.0 0.0 0.5794180213777346 0.0 36-37 0.0 0.0 0.0 0.9382304278173176 0.0 38-39 0.0 0.0 0.0 1.3321690259211598 0.0 40-41 0.0 0.0 0.0 1.7096935157706752 0.0 42-43 0.0 0.0 0.0 2.0182787509520184 0.0 44-45 0.0 0.0 0.0 2.3258791396381016 0.0 46-47 0.0 0.0 0.0 2.6656511805026657 0.0 48-49 0.0 0.0 0.0 2.977519237334874 0.0 50-51 0.0 0.0 0.0 3.289715576332169 0.0 52-53 0.0 0.0 0.0 3.572366520471676 0.0 54-55 0.0 0.0 0.0 3.90261837854873 0.0 56-57 0.0 0.0 0.0 4.345471019250466 0.0 58-59 0.0 0.0 0.0 4.719712687449117 0.0 60-61 0.0 0.0 0.0 5.071959450586968 0.0 62-63 0.0 0.0 0.0 5.444231425795099 0.0 64-65 0.0 0.0 0.0 5.862791186280432 0.0 66-67 0.0 0.0 0.0 6.332891246684349 0.0 68-69 0.0 0.0 0.0 6.839430627412874 0.0 70-71 0.0 0.0 0.0 7.359101294744859 0.0 72-73 0.0 0.0 0.0 7.895186070331171 0.0 74-75 0.0 0.0 0.0 8.448013236336896 0.0 76-77 0.0 0.0 0.0 9.074047325156918 0.0 78-79 0.0 0.0 0.0 9.721419754707565 0.0 80-81 0.0 0.0 0.0 10.414423405205241 0.0 82-83 0.0 0.0 0.0 11.106442209207659 0.0 84-85 0.0 0.0 0.0 11.814546839299314 0.0 86-87 0.0 0.0 0.0 12.591590724059143 0.0 88 0.0 0.0 0.0 13.215983402053734 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCTAAG 20 0.0021583452 70.508385 3 GTACATA 25 0.0052180793 56.42525 1 GTATAAC 25 0.0052180793 56.42525 1 TACATGA 60 2.5340341E-8 54.83985 2 TACAAGA 60 2.5340341E-8 54.83985 2 CTAACAA 35 2.9276783E-4 53.720673 8 CAGGCTA 55 5.257732E-5 42.732353 4 TCAACAC 205 0.0 41.273197 3 GTACAAG 80 2.4312612E-7 41.143414 1 GAGAACA 260 0.0 39.78704 1 CGTATCA 25 0.0017045642 37.57976 90-91 TTAGACC 65 1.4067489E-4 36.158142 4 AGAACAA 265 0.0 35.475914 2 GCTAACA 55 0.0027080588 34.185883 7 GCCGTAT 100 1.4064353E-6 32.882294 94 ACTATTA 60 0.0041473308 31.337057 3 TACAGTG 60 0.0041473308 31.337057 7 TCTATTC 60 0.0041473308 31.337057 3 ATACGGC 30 0.0041604894 31.32676 82-83 GGCTAAC 60 0.004154012 31.32676 6 >>END_MODULE