FastQCFastQC Report
Wed 25 May 2016
SRR1033033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152308
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19651.2901489087900833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18371.2061086745279301No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT16401.0767655014838353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.6559077658428972No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT7400.48585760432807207RNA PCR Primer, Index 26 (100% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.35191848097276573No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT4290.2816660976442472RNA PCR Primer, Index 26 (100% over 24bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.23964598051317065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.2350500302019592No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3170.2081308926648633No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.20484807101399793No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.16939359718465216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2070.13590881634582558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2070.13590881634582558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1930.12671691572340257No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1850.12146440108201802No Hit
TCCTTATACTGAATCCATTTCTCTACTTTTCAGGTAAGTGAAAGGGGTCA1660.10898967880872967No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTAG451.6078227E-552.2193038
GTTCTAG405.5897096E-447.121131
TAGGGAG502.9980401E-546.9973755
TAGACCC405.6626915E-446.997375
GGTATCA12800.044.1760641
ATTAGGG555.2529933E-542.7389143
TCTAGAC450.001009828641.789163
TACCCAT704.548445E-640.362972
CTAGACC608.7739594E-539.177344
CCTCTAC853.847108E-738.8056371
TCTACAG651.4054848E-436.16373
TCATTAG753.213409E-431.414091
AAAGTAC600.004144227631.3418713
GTATCAA18850.029.97782
GACACGT552.9603034E-629.9074262-63
ACCCATG953.7242404E-529.6922973
ACACCAA804.7632158E-429.3733585
CTGAACA650.006139323628.9214694
CTCTACA1205.713835E-627.4692422
TAGGGCG605.8272526E-627.41513454-55
Produced by FastQC (version 0.11.5)