##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033033_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 152308 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.498883840638705 33.0 31.0 34.0 31.0 34.0 2 32.468951072826115 34.0 31.0 34.0 31.0 34.0 3 32.52472621267432 34.0 31.0 34.0 30.0 34.0 4 36.118188145074456 37.0 35.0 37.0 35.0 37.0 5 36.11784673162276 37.0 35.0 37.0 35.0 37.0 6 36.230217716731886 37.0 37.0 37.0 35.0 37.0 7 36.170621372482074 37.0 37.0 37.0 35.0 37.0 8 36.20298342831631 37.0 37.0 37.0 35.0 37.0 9 37.94655566352391 39.0 38.0 39.0 35.0 39.0 10-11 37.91655395645665 39.0 38.0 39.0 35.0 39.0 12-13 37.876913885022454 39.0 38.0 39.0 35.0 39.0 14-15 39.203328124589646 41.0 39.0 41.0 36.0 41.0 16-17 39.20492357591196 41.0 39.0 41.0 36.0 41.0 18-19 39.31407739580324 41.0 39.0 41.0 36.0 41.0 20-21 39.30768245922735 41.0 39.0 41.0 36.0 41.0 22-23 39.160201696562225 41.0 39.0 41.0 35.0 41.0 24-25 39.03514917141581 41.0 39.0 41.0 35.0 41.0 26-27 38.794662788560025 40.5 38.0 41.0 35.0 41.0 28-29 38.645524200961205 40.0 38.0 41.0 35.0 41.0 30-31 38.34323213488457 40.0 37.5 41.0 35.0 41.0 32-33 37.95355135646191 40.0 36.5 41.0 34.0 41.0 34-35 37.65519539354466 40.0 35.5 41.0 33.0 41.0 36-37 37.37242955064737 40.0 35.0 41.0 33.0 41.0 38-39 37.05808952911207 40.0 35.0 41.0 33.0 41.0 40-41 36.76591183654175 40.0 35.0 41.0 32.5 41.0 42-43 36.37466515219161 40.0 35.0 41.0 31.0 41.0 44-45 35.79808348872022 40.0 35.0 41.0 28.5 41.0 46-47 34.95037358510387 40.0 35.0 41.0 18.0 41.0 48-49 34.18500013131287 40.0 35.0 41.0 2.0 41.0 50-51 33.72735509625233 39.5 35.0 41.0 2.0 41.0 52-53 33.519529506001 39.0 35.0 41.0 2.0 41.0 54-55 33.25726816713502 39.0 35.0 41.0 2.0 41.0 56-57 33.05327691257189 39.0 34.0 41.0 2.0 41.0 58-59 32.83938466790976 38.5 34.0 41.0 2.0 41.0 60-61 32.57563621083594 37.5 34.0 41.0 2.0 41.0 62-63 32.28292341833653 37.0 33.5 40.5 2.0 41.0 64-65 31.91765041888804 37.0 33.0 40.0 2.0 41.0 66-67 31.459995535362552 36.0 33.0 39.5 2.0 41.0 68-69 30.944116527037316 35.5 32.0 39.0 2.0 41.0 70-71 30.408744123749244 35.0 31.5 39.0 2.0 41.0 72-73 29.97457454631405 35.0 31.0 37.5 2.0 40.5 74-75 29.668658245134857 35.0 31.0 37.0 2.0 39.5 76-77 29.358684376395196 35.0 31.0 37.0 2.0 39.0 78-79 29.042972135409826 35.0 30.5 36.0 2.0 39.0 80-81 28.77260879270951 35.0 30.0 36.0 2.0 37.5 82-83 28.528747669196626 35.0 30.0 35.0 2.0 37.0 84-85 28.355211151088582 35.0 30.0 35.0 2.0 37.0 86-87 28.228980092969508 35.0 30.0 35.0 2.0 36.0 88-89 28.059356698269298 35.0 30.0 35.0 2.0 36.0 90-91 27.913369619455317 35.0 29.0 35.0 2.0 36.0 92-93 27.765068151377474 35.0 29.0 35.0 2.0 35.0 94-95 27.61441946581926 35.0 29.0 35.0 2.0 35.0 96-97 27.480486908107256 35.0 29.0 35.0 2.0 35.0 98-99 27.357039682748116 35.0 29.0 35.0 2.0 35.0 100 27.245587887701237 35.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24246201593712158 1101 2 0.03599387363802009 1101 3 0.2975359639637105 1101 4 0.12078980493718916 1101 5 0.3628314443288332 1101 6 0.042379059974059885 1101 7 0.021706545508955344 1101 8 0.20838581713945814 1101 9 0.5113953499483728 1101 10-11 0.24905515318199178 1101 12-13 0.36532444379137985 1101 14-15 0.25012563339817717 1101 16-17 0.3704040071067993 1101 18-19 0.46622364769623914 1101 20-21 0.4455582706601433 1101 22-23 0.4702689487370151 1101 24-25 0.4652275323095978 1101 26-27 0.6192524227738829 1101 28-29 0.44124887767967635 1101 30-31 0.6549900554591659 1101 32-33 0.5885644465665649 1101 34-35 0.6400925818435539 1101 36-37 0.42378948023601737 1101 38-39 0.5823532770498119 1101 40-41 0.6462748605149073 1101 42-43 0.8094129991575443 1101 44-45 0.9082259312666636 1101 46-47 0.6585904688310009 1101 48-49 0.8556532450579013 1101 50-51 0.49291587620798794 1101 52-53 0.8637321397437319 1101 54-55 0.9763388803750317 1101 56-57 0.6727681871058024 1101 58-59 0.6035047339686201 1101 60-61 0.592877629750646 1101 62-63 0.8957374835485084 1101 64-65 0.9311510190816499 1101 66-67 0.8944497413422035 1101 68-69 0.7864061788085159 1101 70-71 0.6854759020399506 1101 72-73 1.0043648589279393 1101 74-75 0.812996871281289 1101 76-77 1.0370230821360806 1101 78-79 0.9161809755692865 1101 80-81 0.8344963200714801 1101 82-83 0.9169396876599585 1101 84-85 0.913534801429897 1101 86-87 0.7936051535657818 1101 88-89 1.0175364237485418 1101 90-91 0.8817629224049384 1101 92-93 0.6487839461042881 1101 94-95 0.6834340491125985 1101 96-97 0.6332965621520437 1101 98-99 0.5911778723611327 1101 100 0.469566027275615 1102 1 0.18436906721917268 1102 2 0.2998560531251968 1102 3 0.2592346819124316 1102 4 0.16349813827051918 1102 5 0.09400131612370188 1102 6 0.1712252138202146 1102 7 0.19806872499613348 1102 8 0.13995632995997198 1102 9 0.2632382986663231 1102 10-11 0.2899205377973715 1102 12-13 0.3922875848170264 1102 14-15 0.47099903083407213 1102 16-17 0.43017964813244447 1102 18-19 0.22283422461931934 1102 20-21 0.25914000142937965 1102 22-23 0.26490035899342246 1102 24-25 0.23393746820703853 1102 26-27 0.2636033843123471 1102 28-29 0.16256298024377713 1102 30-31 0.04393236315147675 1102 32-33 0.08740258759220154 1102 34-35 -0.19316062328464767 1102 36-37 -0.006619173610133089 1102 38-39 0.15939654628057554 1102 40-41 0.2242796682072168 1102 42-43 0.4837319094139616 1102 44-45 0.5928813799846111 1102 46-47 0.4978933534463792 1102 48-49 0.7107814501861114 1102 50-51 0.8144302992849077 1102 52-53 0.6317209217950079 1102 54-55 0.6595520214006712 1102 56-57 0.7865502383878606 1102 58-59 0.7830319775583661 1102 60-61 0.4563792323147453 1102 62-63 0.7437743425228689 1102 64-65 0.7561910831842162 1102 66-67 0.816364805444767 1102 68-69 0.5557571403469765 1102 70-71 0.43884128665533595 1102 72-73 0.6157029999535837 1102 74-75 0.7394792430761612 1102 76-77 0.6339782103412084 1102 78-79 0.6377354627487719 1102 80-81 0.32071426878943043 1102 82-83 0.7594877645830351 1102 84-85 0.645906596301689 1102 86-87 0.8224913715145021 1102 88-89 0.8428569365690528 1102 90-91 0.4576843967639128 1102 92-93 0.7638480486683932 1102 94-95 0.5131616132151642 1102 96-97 0.5119423954853808 1102 98-99 0.25704325697651953 1102 100 0.3238128221474099 1103 1 -0.0487311658810583 1103 2 -0.025085671816526656 1103 3 -0.050633227955479754 1103 4 0.018043592815978116 1103 5 0.18685290897529683 1103 6 0.043641686236689736 1103 7 0.14181386874128066 1103 8 0.24026713027077307 1103 9 0.20441933984736238 1103 10-11 0.24873949661633077 1103 12-13 0.17600941853886098 1103 14-15 0.20033081016585186 1103 16-17 0.31390148185428046 1103 18-19 0.06005256183765084 1103 20-21 0.10614854843792187 1103 22-23 -0.017578273485206353 1103 24-25 0.024807709077272477 1103 26-27 -0.20753103682207552 1103 28-29 -0.663233445552649 1103 30-31 -0.9349720657529588 1103 32-33 -0.9894816392920234 1103 34-35 -0.7461132262372558 1103 36-37 -0.5350962520872073 1103 38-39 -0.7734705865865621 1103 40-41 -0.7682611243335771 1103 42-43 -0.930564904882857 1103 44-45 -0.9351294980262637 1103 46-47 -0.7968230412700095 1103 48-49 -0.6014174620127974 1103 50-51 -0.7828890480344413 1103 52-53 -1.1235626834885863 1103 54-55 -1.1602614984128437 1103 56-57 -0.7445297926921768 1103 58-59 -0.7600527155263279 1103 60-61 -0.890629314693804 1103 62-63 -0.8480282992797754 1103 64-65 -1.2291236021304677 1103 66-67 -1.148242809162852 1103 68-69 -0.9431550585652246 1103 70-71 -0.7573902595762085 1103 72-73 -0.7510802168296316 1103 74-75 -0.8343358307389153 1103 76-77 -0.7644289380659401 1103 78-79 -0.9706561456428382 1103 80-81 -0.6370945890194264 1103 82-83 -0.5750110699157958 1103 84-85 -0.4418944914993972 1103 86-87 -0.4298007807776507 1103 88-89 -0.5992565055443855 1103 90-91 -0.4813210422415253 1103 92-93 -0.27356453874419273 1103 94-95 -0.37164802159447063 1103 96-97 -0.5299767964338109 1103 98-99 -0.25636000642674617 1103 100 -0.19631149797691094 1104 1 0.387212428478378 1104 2 0.18373521027168493 1104 3 0.32610444581190023 1104 4 0.08990077853454892 1104 5 0.16115033871827222 1104 6 0.0675440764757127 1104 7 0.32353204055846163 1104 8 0.2060137049282389 1104 9 0.47854675258864177 1104 10-11 0.36405102776944887 1104 12-13 0.33079989758675765 1104 14-15 0.6299987769625304 1104 16-17 0.490969188624959 1104 18-19 0.4008616427457383 1104 20-21 0.4958625198350646 1104 22-23 0.36718520286243006 1104 24-25 0.4239476230686776 1104 26-27 0.6093256488464931 1104 28-29 0.7722100988017928 1104 30-31 0.5558770191555311 1104 32-33 0.785320840956004 1104 34-35 0.6114725323386025 1104 36-37 0.8119134488828834 1104 38-39 0.6215189123633351 1104 40-41 1.077973499128774 1104 42-43 1.1054887004776788 1104 44-45 1.085747589682505 1104 46-47 1.2842350645405674 1104 48-49 1.4703397137047673 1104 50-51 1.5167659174621093 1104 52-53 1.4001646892486832 1104 54-55 1.4313976675037452 1104 56-57 1.664311091396236 1104 58-59 1.7892772174669744 1104 60-61 1.68912866110378 1104 62-63 1.4793544389940507 1104 64-65 1.1107353837681266 1104 66-67 1.344506465764642 1104 68-69 1.366825939534582 1104 70-71 1.428153294779225 1104 72-73 1.4525651477068173 1104 74-75 1.3569582986740265 1104 76-77 1.466169121740041 1104 78-79 1.443460265998329 1104 80-81 1.5310722276622428 1104 82-83 1.472993730564248 1104 84-85 1.5003747354232928 1104 86-87 1.4410113004304677 1104 88-89 1.4934027603822067 1104 90-91 1.6046125511178104 1104 92-93 1.4819110088105596 1104 94-95 1.8145706002677144 1104 96-97 1.6124374449903307 1104 98-99 1.6059262221961177 1104 100 1.592967429915877 1105 1 0.20575684216144907 1105 2 0.2786627125005765 1105 3 0.4035292499036842 1105 4 0.2901964005138069 1105 5 0.2158873290049783 1105 6 0.37630481036900676 1105 7 0.008155005224594447 1105 8 0.03535066706047019 1105 9 0.11894373067507047 1105 10-11 0.21652158288183188 1105 12-13 0.28380538770923636 1105 14-15 0.2640844604123984 1105 16-17 0.4335457922568793 1105 18-19 0.28809124248296314 1105 20-21 0.3773439144200168 1105 22-23 0.3353706469992588 1105 24-25 0.165970522041043 1105 26-27 0.04550884062538785 1105 28-29 0.2413574874743034 1105 30-31 0.07684037141492439 1105 32-33 0.48697726252232343 1105 34-35 0.27168322165397996 1105 36-37 0.2015713720211707 1105 38-39 0.1059635653594384 1105 40-41 0.3966821472447606 1105 42-43 0.43161136013701196 1105 44-45 0.6967700663377556 1105 46-47 0.6390283637757008 1105 48-49 1.104073466226943 1105 50-51 0.864497136081603 1105 52-53 1.020759687137101 1105 54-55 1.2588107405646056 1105 56-57 0.9706552329193912 1105 58-59 1.087151554663734 1105 60-61 1.2189020175863448 1105 62-63 1.2013876612736283 1105 64-65 0.8810300672649127 1105 66-67 0.8813301718319302 1105 68-69 1.176525155901718 1105 70-71 1.2411137377429533 1105 72-73 1.2824182751996638 1105 74-75 0.999595903666755 1105 76-77 1.4133560205247093 1105 78-79 1.326701315221733 1105 80-81 0.6337656723621627 1105 82-83 0.891885382762645 1105 84-85 0.8115403306558022 1105 86-87 0.7896927325547267 1105 88-89 0.7826430588196338 1105 90-91 0.9051263702722352 1105 92-93 1.2108890013965485 1105 94-95 1.4056151312611007 1105 96-97 1.2508049547270836 1105 98-99 1.249995012961449 1105 100 1.226255403262975 1106 1 0.08906185870441874 1106 2 0.18546321326066106 1106 3 0.09726080673100768 1106 4 0.15154845273593054 1106 5 0.07755235627851675 1106 6 0.17524069519419072 1106 7 0.13461928135801315 1106 8 0.07650688632185165 1106 9 0.05257066441378555 1106 10-11 0.31961332928261754 1106 12-13 0.1863248368731476 1106 14-15 0.3773367183038374 1106 16-17 0.5058206737734707 1106 18-19 0.40298661939435476 1106 20-21 0.35981731154064533 1106 22-23 0.35165652735336295 1106 24-25 0.23326015809576717 1106 26-27 0.515429702648099 1106 28-29 0.4493070537803021 1106 30-31 0.3764916277862014 1106 32-33 0.4328549339879473 1106 34-35 0.8284770158058166 1106 36-37 0.9157804393555224 1106 38-39 0.8126500537793717 1106 40-41 0.7519647190054712 1106 42-43 0.6165572601638374 1106 44-45 0.7842577708893046 1106 46-47 1.2069886606548934 1106 48-49 1.3076972702151366 1106 50-51 1.0430829359564058 1106 52-53 0.9826885076682572 1106 54-55 1.0510735501863522 1106 56-57 1.1542933443385124 1106 58-59 0.8160145610126506 1106 60-61 0.6961107272349238 1106 62-63 0.5503660212843542 1106 64-65 0.905453298955873 1106 66-67 0.9124823671283657 1106 68-69 0.6047532700034814 1106 70-71 1.0205781032978152 1106 72-73 0.9858408809405184 1106 74-75 0.8802629876334898 1106 76-77 1.1449240255323048 1106 78-79 1.1214679252548194 1106 80-81 0.9166504081217965 1106 82-83 1.1346087128171902 1106 84-85 1.0577958077192022 1106 86-87 0.8528130938173533 1106 88-89 0.9856968011070322 1106 90-91 1.115400894103054 1106 92-93 1.2553091033369945 1106 94-95 1.211637665464803 1106 96-97 0.6713763577495335 1106 98-99 0.7442832182730825 1106 100 0.9075573794768701 1107 1 -0.15747345603927698 1107 2 -0.16104705556551835 1107 3 -0.2377700832339258 1107 4 0.08267217956845485 1107 5 0.1278111640928401 1107 6 0.13566814551978723 1107 7 -0.02655119785387683 1107 8 0.08347800003031836 1107 9 0.1938713823967504 1107 10-11 0.1250691649813973 1107 12-13 0.1400525042633589 1107 14-15 -0.027321822354032577 1107 16-17 -0.21099348551857133 1107 18-19 -0.16161097619756504 1107 20-21 -0.12590651547554188 1107 22-23 -0.13458908961106175 1107 24-25 -0.02880362550751414 1107 26-27 -0.13215336631165542 1107 28-29 0.29126731425784413 1107 30-31 -0.05417292389186912 1107 32-33 -0.09757585579331618 1107 34-35 0.03541890927717617 1107 36-37 0.22451311592923417 1107 38-39 0.42571149976367195 1107 40-41 0.0816316083025157 1107 42-43 0.04500942246138351 1107 44-45 -0.5213232943970567 1107 46-47 0.00678829331477715 1107 48-49 0.02453230110510418 1107 50-51 0.1826513113112327 1107 52-53 -0.0348436345925478 1107 54-55 0.2804732830298988 1107 56-57 0.11874901306451591 1107 58-59 0.07292305992733361 1107 60-61 0.3075024453848556 1107 62-63 0.45249909859276016 1107 64-65 0.46804722114655206 1107 66-67 0.8838256631711126 1107 68-69 1.0471685313158936 1107 70-71 0.6203025982051464 1107 72-73 0.5982761789869393 1107 74-75 0.43110620018984136 1107 76-77 0.8575429936405072 1107 78-79 0.7884615802890487 1107 80-81 0.7093119542897739 1107 82-83 0.7452490534416718 1107 84-85 0.5508873088045263 1107 86-87 0.6844836565156349 1107 88-89 0.9185319989697796 1107 90-91 0.835095333909365 1107 92-93 0.5593665419744944 1107 94-95 0.5567286656317734 1107 96-97 0.4296415105296276 1107 98-99 0.545731707169395 1107 100 0.4764336526443458 1108 1 -0.2534072369364395 1108 2 -0.1089414711895671 1108 3 -0.15038070270295378 1108 4 0.01752112677626627 1108 5 -0.07664413038381213 1108 6 -0.04670139979604926 1108 7 0.08582292481929699 1108 8 -0.01970326332030936 1108 9 0.06078471776447003 1108 10-11 -0.06595921463410548 1108 12-13 0.22137688632012242 1108 14-15 0.18089071960507397 1108 16-17 0.15883791515278034 1108 18-19 0.1265145959720968 1108 20-21 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-0.5677548970459299 2215 28-29 -0.7088074286266348 2215 30-31 -0.8193905751714681 2215 32-33 -0.973442873253255 2215 34-35 -1.0326270127510426 2215 36-37 -0.9435387855297463 2215 38-39 -0.9058927815087472 2215 40-41 -1.192196423268875 2215 42-43 -1.3547902666082194 2215 44-45 -1.0058365687333364 2215 46-47 -1.2929157248627021 2215 48-49 -1.1736260342213782 2215 50-51 -1.6722727499181431 2215 52-53 -1.3983033332292365 2215 54-55 -1.3118790250303718 2215 56-57 -1.5321867678491472 2215 58-59 -1.6371343426079505 2215 60-61 -1.4642028882673799 2215 62-63 -1.5652000164514916 2215 64-65 -1.3263799493868156 2215 66-67 -1.0532557766758188 2215 68-69 -1.1691822220923846 2215 70-71 -1.1374407646267137 2215 72-73 -0.9872595780089952 2215 74-75 -0.8395228359259193 2215 76-77 -0.7915242151612176 2215 78-79 -0.7148719523586458 2215 80-81 -0.903116292541128 2215 82-83 -0.9041470240517526 2215 84-85 -0.9502039373088422 2215 86-87 -1.1176420311189013 2215 88-89 -0.8388824951703775 2215 90-91 -0.6872826857031704 2215 92-93 -0.7081980358776931 2215 94-95 -0.9186211310675816 2215 96-97 -0.5424494964065154 2215 98-99 -0.41360816367490116 2215 100 -0.4627256393910528 2216 1 -0.16234219253556148 2216 2 -0.2488618955927464 2216 3 -0.14745428129827332 2216 4 -0.13995782605501006 2216 5 -0.04089277529212865 2216 6 0.07512710902646091 2216 7 0.009216997013972161 2216 8 -0.012106100363325822 2216 9 -0.18960451069822426 2216 10-11 -0.0634796851680619 2216 12-13 -0.2599978109466292 2216 14-15 -0.39700542847473486 2216 16-17 -0.5318047442867311 2216 18-19 -0.2805102569860338 2216 20-21 -0.2597005782807642 2216 22-23 -0.2036615139162734 2216 24-25 -0.12381058084536534 2216 26-27 -0.026920584472492237 2216 28-29 -0.011127097794130236 2216 30-31 -0.044250468509623886 2216 32-33 -0.03377912924168669 2216 34-35 -0.24990532785543706 2216 36-37 0.1690775933909876 2216 38-39 0.3716596455001948 2216 40-41 0.5175907049965147 2216 42-43 0.514724105623543 2216 44-45 0.3227587489924204 2216 46-47 -0.003555921915939564 2216 48-49 -0.12567339931221255 2216 50-51 0.28823186004298407 2216 52-53 0.5366261614828645 2216 54-55 0.04294109609409702 2216 56-57 0.15778769658183478 2216 58-59 0.27834970331088016 2216 60-61 0.4807939480338064 2216 62-63 0.3536963046187438 2216 64-65 -0.05662942963629192 2216 66-67 0.03743503955847416 2216 68-69 -0.2096832163977318 2216 70-71 0.33371308152713297 2216 72-73 0.33275985614087134 2216 74-75 0.23279027986545486 2216 76-77 -0.03268845632545947 2216 78-79 -0.33160678920217634 2216 80-81 -0.30269710244802894 2216 82-83 -0.11397934801451015 2216 84-85 -0.256546023542235 2216 86-87 -0.23848967642306818 2216 88-89 -0.26360906120129 2216 90-91 -0.35112273813017936 2216 92-93 -0.45733366816550003 2216 94-95 -0.37814273461092185 2216 96-97 -0.4877231563540825 2216 98-99 -0.35053756799560887 2216 100 -0.3912373450766715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 16.0 6 65.0 7 118.0 8 162.0 9 243.0 10 370.0 11 644.0 12 902.0 13 1327.0 14 1441.0 15 1900.0 16 2539.0 17 3748.0 18 4316.0 19 1970.0 20 880.0 21 674.0 22 742.0 23 830.0 24 982.0 25 1312.0 26 1738.0 27 2019.0 28 1269.0 29 1529.0 30 1828.0 31 2302.0 32 2868.0 33 3607.0 34 4962.0 35 8273.0 36 13821.0 37 27362.0 38 41253.0 39 14284.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.66726660054257 19.164603025505947 14.441765907553256 21.72636446639823 2 16.799747223739903 21.88160328609138 38.4897935002271 22.828855989941612 3 18.638503509451553 27.225990295660623 32.261347445552616 21.87415874933521 4 12.712493187742533 19.62127131497909 35.46050860478401 32.20572689249437 5 12.042125444004544 36.958248800120806 35.037128956646775 15.962496799227877 6 28.091104866454813 37.98093337185177 16.295926674895604 17.632035086797803 7 23.46954854636657 35.898311316542795 22.046117078551354 18.586023058539276 8 25.14920522366011 32.82711891114656 21.36868298896308 20.654992876230246 9 25.753735851038684 14.770727735903563 21.34753263124721 38.12800378181054 10-11 24.133414309866552 26.591927515059993 26.940564318894346 22.33409385617911 12-13 26.654870394201225 24.784646899703233 28.23751871208341 20.322963994012134 14-15 23.279801454946554 25.12967145520918 28.833679123880557 22.756847965963704 16-17 19.199910707251096 28.61110381595189 29.6908238569215 22.498161619875514 18-19 19.47140005777766 24.237728812669065 35.85038212044016 20.440489009113115 20-21 20.739554061506947 22.616671481471755 36.56144129001759 20.082333167003704 22-23 20.18127860177142 23.753996861601895 36.44415555424242 19.620568982384263 24-25 20.270838120875876 23.886609106726635 36.63110206493549 19.211450707462 26-27 19.854505344433647 23.0811907450692 37.460277858024526 19.60402605247262 28-29 19.280403131822137 22.550104229929584 38.48464455131888 19.684848086929403 30-31 20.300312524621162 22.728943981931348 36.5197494550516 20.45099403839588 32-33 20.291777188328915 22.79066102896762 37.492777792368095 19.424783990335374 34-35 19.9677626913885 22.99321112482601 37.4806313522599 19.558394831525593 36-37 19.68970769756021 23.207579378627518 36.843107387661846 20.25960553615043 38-39 19.741904561808965 22.11702602621005 37.247879297213544 20.893190114767446 40-41 19.786550936260735 22.618641174462272 37.196010715129866 20.398797174147123 42-43 20.324605404837566 23.000105050292827 35.98005357564934 20.695235969220263 44-45 20.278317619560365 23.38550831210442 35.64816030674686 20.688013761588362 46-47 21.62722903590094 22.98270609554324 33.902684034981746 21.487380833574075 48-49 21.34753263124721 23.338892244662134 32.55508574730152 22.758489376789136 50-51 20.50483231347007 23.748588386690127 32.69854505344434 23.048034246395463 52-53 21.23952779893374 23.66947238490427 32.68016125219949 22.410838563962496 54-55 20.76483178821861 24.130708826850856 31.71369855818473 23.390760826745804 56-57 21.385941644562333 24.26399138587599 30.726882369934604 23.623184599627074 58-59 20.17293904456759 24.912676944086982 31.546931218320772 23.36745279302466 60-61 20.830159939070832 25.8752002521207 30.023045407989073 23.271594400819392 62-63 20.268797436772857 25.935604170496624 30.324736717703598 23.470861675026917 64-65 19.576450350605352 26.42835570029152 30.17044410011293 23.824749848990205 66-67 20.079378627517926 26.05181605693726 29.940646584552354 23.928158730992465 68-69 19.923772881266906 25.93527588833154 30.053903931507207 24.087047298894344 70-71 20.15849462930378 25.357827559944322 30.059156446148595 24.424521364603304 72-73 20.295716574309953 25.127701762218663 30.000722220763194 24.575859442708197 74-75 20.04655041100927 24.897904246658086 29.62845024555506 25.42709509677758 76-77 20.592811933713264 24.38611235128818 29.551303936759727 25.46977177823883 78-79 20.932255692412742 24.086390734564173 29.91438401134543 25.066969561677656 80-81 20.553418073902883 23.905178979436407 29.765672190561233 25.775730756099485 82-83 20.49071618037135 24.159269375213384 29.538172650156262 25.811841794259 84-85 20.655185545079707 24.379874990151535 28.932820337736693 26.032119127032065 86-87 20.869882081046303 24.471465714210677 28.5766341886178 26.082018016125218 88-89 20.52644019276982 24.413023781072315 28.400391317479283 26.66014470867858 90-91 20.781587007691776 23.869131449619353 28.435611320668 26.913670222020876 92-93 20.515076407924756 24.073995042923034 28.49006122482478 26.92086732432743 94-95 20.33740840927594 24.30207211702603 28.640320928644584 26.720198545053442 96-97 20.790109514930272 23.837881135593666 28.557922105207865 26.814087244268194 98-99 20.744478293983246 23.957375843685163 27.975877826509443 27.32226803582215 100 20.73167528954487 22.962680883472963 28.169892586075584 28.135751240906583 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 205.0 1 141.5 2 163.0 3 243.5 4 148.0 5 80.0 6 188.0 7 359.0 8 542.0 9 613.0 10 407.0 11 179.5 12 131.0 13 123.5 14 131.0 15 152.5 16 157.5 17 172.5 18 199.5 19 216.5 20 253.0 21 269.0 22 317.0 23 428.5 24 515.5 25 612.0 26 813.5 27 1143.0 28 1529.5 29 1799.5 30 1948.0 31 2258.0 32 2553.5 33 3091.0 34 3766.5 35 4013.5 36 4267.0 37 4584.5 38 4749.0 39 4857.5 40 4833.5 41 4721.5 42 4878.0 43 5362.0 44 5605.5 45 5417.0 46 5336.5 47 5268.0 48 5210.5 49 5237.5 50 5210.5 51 5254.5 52 5009.0 53 4533.5 54 3974.5 55 3464.0 56 3049.0 57 2778.0 58 2679.5 59 2468.5 60 2292.0 61 2219.0 62 1883.0 63 1456.0 64 1283.5 65 1063.0 66 761.5 67 533.5 68 508.0 69 476.0 70 297.0 71 217.0 72 230.0 73 188.5 74 149.0 75 103.5 76 39.5 77 19.5 78 12.0 79 19.0 80 16.5 81 9.5 82 12.5 83 7.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04530293878194185 2 0.25934291041836277 3 0.003282821650865352 4 0.005909078971557633 5 6.565643301730704E-4 6 0.0 7 0.0 8 6.565643301730704E-4 9 0.0 10-11 3.282821650865352E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.565643301730704E-4 24-25 0.0019696929905192112 26-27 0.0 28-29 3.282821650865352E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0013131286603461408 90-91 0.001641410825432676 92-93 3.282821650865352E-4 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 152308.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.96961420279959 #Duplication Level Percentage of deduplicated Percentage of total 1 73.89423076923077 30.274181264280276 2 9.762820512820513 7.999579798828689 3 4.328525641025641 5.320140767392389 4 2.5544871794871793 4.1862541691834965 5 1.7868589743589742 3.6603461407148674 6 1.3461538461538463 3.3090842240722744 7 1.0224358974358976 2.9322162985529325 8 0.7708333333333334 2.526459542505975 9 0.6490384615384616 2.3931769834808416 >10 3.7211538461538463 26.202825852877066 >50 0.1201923076923077 3.3465083908921396 >100 0.03365384615384615 2.782519631273472 >500 0.004807692307692308 1.493683851143735 >1k 0.004807692307692308 3.573023084801849 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1965 1.2901489087900833 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1837 1.2061086745279301 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 1640 1.0767655014838353 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 999 0.6559077658428972 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 740 0.48585760432807207 RNA PCR Primer, Index 26 (100% over 22bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 536 0.35191848097276573 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 429 0.2816660976442472 RNA PCR Primer, Index 26 (100% over 24bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 365 0.23964598051317065 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 358 0.2350500302019592 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 317 0.2081308926648633 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 312 0.20484807101399793 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 258 0.16939359718465216 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 207 0.13590881634582558 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 207 0.13590881634582558 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 193 0.12671691572340257 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 185 0.12146440108201802 No Hit TCCTTATACTGAATCCATTTCTCTACTTTTCAGGTAAGTGAAAGGGGTCA 166 0.10898967880872967 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.565643301730703E-4 0.0 16-17 0.0 0.0 0.0 9.848464952596054E-4 0.0 18-19 0.0 0.0 0.0 0.0019696929905192112 0.0 20-21 0.0 0.0 0.0 0.002297975155605746 0.0 22-23 0.0 0.0 0.0 0.0062373611366441685 0.0 24-25 0.0 0.0 0.0 0.014116133098721013 0.0 26-27 0.0 0.0 0.0 0.03840901331512461 0.0 28-29 0.0 0.0 0.0 0.0810856947763742 0.0 30-31 0.0 0.0 0.0 0.17661580481655592 0.0 32-33 0.0 0.0 0.0 0.3210599574546314 0.0 34-35 0.0 0.0 0.0 0.5804028678729942 0.0 36-37 0.0 0.0 0.0 0.9428263781285291 0.0 38-39 0.0 0.0 0.0 1.337749822727631 0.0 40-41 0.0 0.0 0.0 1.7182288520629252 0.0 42-43 0.0 0.0 0.0 2.0304251910602202 0.0 44-45 0.0 0.0 0.0 2.337040733251044 0.0 46-47 0.0 0.0 0.0 2.677469338445781 0.0 48-49 0.0 0.0 0.0 2.993933345589201 0.0 50-51 0.0 0.0 0.0 3.313023610053313 0.0 52-53 0.0 0.0 0.0 3.6022401974945506 0.0 54-55 0.0 0.0 0.0 3.9380728523780757 0.0 56-57 0.0 0.0 0.0 4.390445675867321 0.0 58-59 0.0 0.0 0.0 4.768955012212096 0.0 60-61 0.0 0.0 0.0 5.126454289991333 0.0 62-63 0.0 0.0 0.0 5.502009086850329 0.0 64-65 0.0 0.0 0.0 5.924836515481787 0.0 66-67 0.0 0.0 0.0 6.401502219187436 0.0 68-69 0.0 0.0 0.0 6.922486015179768 0.0 70-71 0.0 0.0 0.0 7.452661711794521 0.0 72-73 0.0 0.0 0.0 7.99761010583817 0.0 74-75 0.0 0.0 0.0 8.5527352469995 0.0 76-77 0.0 0.0 0.0 9.180410746644956 0.0 78-79 0.0 0.0 0.0 9.833692255167161 0.0 80-81 0.0 0.0 0.0 10.539498910103212 0.0 82-83 0.0 0.0 0.0 11.247603540194868 0.0 84-85 0.0 0.0 0.0 11.964900070908946 0.0 86-87 0.0 0.0 0.0 12.756388370932584 0.0 88 0.0 0.0 0.0 13.379467920266828 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGTAG 45 1.6078227E-5 52.219303 8 GTTCTAG 40 5.5897096E-4 47.12113 1 TAGGGAG 50 2.9980401E-5 46.997375 5 TAGACCC 40 5.6626915E-4 46.99737 5 GGTATCA 1280 0.0 44.176064 1 ATTAGGG 55 5.2529933E-5 42.738914 3 TCTAGAC 45 0.0010098286 41.78916 3 TACCCAT 70 4.548445E-6 40.36297 2 CTAGACC 60 8.7739594E-5 39.17734 4 CCTCTAC 85 3.847108E-7 38.805637 1 TCTACAG 65 1.4054848E-4 36.1637 3 TCATTAG 75 3.213409E-4 31.41409 1 AAAGTAC 60 0.0041442276 31.341871 3 GTATCAA 1885 0.0 29.9778 2 GACACGT 55 2.9603034E-6 29.90742 62-63 ACCCATG 95 3.7242404E-5 29.692297 3 ACACCAA 80 4.7632158E-4 29.373358 5 CTGAACA 65 0.0061393236 28.92146 94 CTCTACA 120 5.713835E-6 27.469242 2 TAGGGCG 60 5.8272526E-6 27.415134 54-55 >>END_MODULE