FastQCFastQC Report
Wed 25 May 2016
SRR1033032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942555
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55700.034.774521
TCAACGC52450.034.326414
CAACGCA54900.032.7942055
ATCAACG55800.032.349843
GGTATCA38200.031.6292481
AACGCAG58900.030.5612146
TATCAAC68550.027.1558152
GTACATG128450.025.8764321
TACATGG127450.024.8965442
ACGCAGA71900.024.644097
CGCAGAG74650.023.31448
ACATGGG133600.022.8004633
CATGGGG101900.019.8366994
GAGTACA94250.019.8028761
AGTACAT97600.017.9652842
GCAGAGT96050.017.436799
CCTATAC16750.017.400113
GTATATA33700.015.7640451
AGAGTAC80900.015.41499510-11
CTATACT22600.015.3921184
Produced by FastQC (version 0.11.5)