FastQCFastQC Report
Wed 25 May 2016
SRR1033032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942555
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63150.12776792569834833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52080.10537060285621506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62100.048.2007641
GTATCAA115350.044.0936131
TCAACGC132850.037.6415144
ATCAACG136400.036.7659533
CAACGCA137150.036.4948845
AACGCAG141650.035.368686
TATCAAC145750.034.462652
ACGCAGA163800.030.3563827
CGCAGAG167200.029.6828698
GTACATG129400.025.8275831
TACATGG125900.025.2626022
GCAGAGT191400.025.242339
ACATGGG132500.023.198383
AGAGTAC172600.021.1163710-11
GAGTACT127850.020.30958412-13
CAGAGTA186600.020.1743310-11
GTGGTAT31050.019.2803061
CATGGGG98900.019.1987134
AGTACTT136750.018.62693412-13
GTACTTT143500.018.07825914-15
Produced by FastQC (version 0.11.5)