##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033032_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4942555
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.741204903132086	34.0	31.0	34.0	31.0	34.0
2	32.74786380728186	34.0	31.0	34.0	31.0	34.0
3	32.85341771614074	34.0	31.0	34.0	31.0	34.0
4	36.32515126285899	37.0	37.0	37.0	35.0	37.0
5	36.309877583557494	37.0	37.0	37.0	35.0	37.0
6	36.37346574797853	37.0	37.0	37.0	35.0	37.0
7	36.32405688960467	37.0	37.0	37.0	35.0	37.0
8	36.3352545394032	37.0	37.0	37.0	35.0	37.0
9	38.14829597242721	39.0	39.0	39.0	37.0	39.0
10-11	38.11250719516525	39.0	39.0	39.0	37.0	39.0
12-13	38.08669594976687	39.0	39.0	39.0	36.0	39.0
14-15	39.594137242782324	41.0	40.0	41.0	37.0	41.0
16-17	39.59337377125799	41.0	40.0	41.0	37.0	41.0
18-19	39.60682430443364	41.0	40.0	41.0	37.0	41.0
20-21	39.59745445422459	41.0	40.0	41.0	37.0	41.0
22-23	39.528168427058475	41.0	39.5	41.0	37.0	41.0
24-25	39.47334587070857	41.0	39.0	41.0	36.0	41.0
26-27	39.36647654907229	41.0	39.0	41.0	36.0	41.0
28-29	39.314676012710024	41.0	39.0	41.0	36.0	41.0
30-31	39.31591302474125	41.0	39.0	41.0	36.0	41.0
32-33	39.28298420553742	41.0	39.0	41.0	35.5	41.0
34-35	39.2075803911135	41.0	39.0	41.0	35.0	41.0
36-37	39.12129536241883	41.0	39.0	41.0	35.0	41.0
38-39	39.029447724911506	41.0	39.0	41.0	35.0	41.0
40-41	38.93043527082652	41.0	39.0	41.0	35.0	41.0
42-43	38.820472407489646	41.0	39.0	41.0	35.0	41.0
44-45	38.665295580929296	41.0	39.0	41.0	35.0	41.0
46-47	38.4830500621642	41.0	38.0	41.0	35.0	41.0
48-49	38.295467121761924	40.5	38.0	41.0	34.0	41.0
50-51	38.161643825915945	40.0	38.0	41.0	34.0	41.0
52-53	38.02327915420264	40.0	38.0	41.0	34.0	41.0
54-55	37.873189676189746	40.0	37.5	41.0	34.0	41.0
56-57	37.71911066240032	40.0	37.0	41.0	33.0	41.0
58-59	37.53698269417336	40.0	37.0	41.0	33.0	41.0
60-61	37.340379014497564	40.0	36.0	41.0	33.0	41.0
62-63	37.10884046813844	39.0	36.0	41.0	33.0	41.0
64-65	36.8393721465922	39.0	35.0	41.0	32.5	41.0
66-67	36.55796688150157	39.0	35.0	41.0	32.0	41.0
68-69	36.25056949290397	38.0	35.0	40.5	32.0	41.0
70-71	35.92047483538372	37.0	35.0	40.0	31.5	41.0
72-73	35.574053500669194	37.0	35.0	39.0	31.0	41.0
74-75	35.22697228457751	36.0	35.0	39.0	31.0	41.0
76-77	34.87067447909027	36.0	35.0	39.0	31.0	40.5
78-79	34.5120346662809	35.0	35.0	37.0	31.0	39.0
80-81	34.1838283033775	35.0	34.0	37.0	30.5	39.0
82-83	33.88781126360759	35.0	34.0	36.5	30.0	39.0
84-85	33.66809130095669	35.0	34.0	36.0	30.0	37.0
86-87	33.465104485433145	35.0	34.0	36.0	30.0	37.0
88-89	33.26112162636531	35.0	34.0	35.5	30.0	37.0
90-91	33.08740014021088	35.0	34.0	35.0	30.0	36.0
92-93	32.92134483076061	35.0	34.0	35.0	30.0	36.0
94-95	32.77460159775663	35.0	34.0	35.0	30.0	36.0
96-97	32.6385099407088	35.0	34.0	35.0	29.5	36.0
98-99	32.52199570869722	35.0	34.0	35.0	29.0	35.5
100	32.427122207036646	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10231464863069561
1101	2	0.08411083656518059
1101	3	0.14408676841289747
1101	4	0.08621061009582576
1101	5	0.10367836818002019
1101	6	0.10138209472382442
1101	7	0.138066228904016
1101	8	0.1650749316269966
1101	9	0.18173042374529302
1101	10-11	0.22176765962299072
1101	12-13	0.20129606301417624
1101	14-15	0.2840404427611105
1101	16-17	0.2889296382208286
1101	18-19	0.27049257174621744
1101	20-21	0.24971630919606724
1101	22-23	0.2264198300037492
1101	24-25	0.29725086103124454
1101	26-27	0.2999478078021909
1101	28-29	0.32155245787489406
1101	30-31	0.37428540669549903
1101	32-33	0.3031001641177724
1101	34-35	0.3044708772804654
1101	36-37	0.3195448688888973
1101	38-39	0.29122614728562013
1101	40-41	0.3049075147986571
1101	42-43	0.32604951863602594
1101	44-45	0.3164162971762039
1101	46-47	0.32127535752903214
1101	48-49	0.3131825810572906
1101	50-51	0.3891536278602672
1101	52-53	0.3770501163023212
1101	54-55	0.37452114403303227
1101	56-57	0.3609678345798244
1101	58-59	0.33839851887810823
1101	60-61	0.3349404137326175
1101	62-63	0.333821909714203
1101	64-65	0.37088126959650225
1101	66-67	0.4162844717769758
1101	68-69	0.3738466050627238
1101	70-71	0.4007804313150558
1101	72-73	0.41044683062960274
1101	74-75	0.39746602368845885
1101	76-77	0.40688687897232967
1101	78-79	0.31204752967179417
1101	80-81	0.3164895786369897
1101	82-83	0.3855685663381436
1101	84-85	0.3630785215678429
1101	86-87	0.371039172455653
1101	88-89	0.3698160824858405
1101	90-91	0.3864349929084483
1101	92-93	0.3737037681610218
1101	94-95	0.3504999511740934
1101	96-97	0.4064916493815076
1101	98-99	0.39846805896275583
1101	100	0.37722427925278623
1102	1	0.12248036058897327
1102	2	0.06268255576972592
1102	3	0.114335127147136
1102	4	0.08210181817553064
1102	5	0.09574542047173651
1102	6	0.12734192835845448
1102	7	0.11111998384534871
1102	8	0.15125558705133813
1102	9	0.19505215044935653
1102	10-11	0.1774910508102181
1102	12-13	0.18566919605435572
1102	14-15	0.2495663853147505
1102	16-17	0.23415962370886234
1102	18-19	0.23203666124720712
1102	20-21	0.21612060558425839
1102	22-23	0.2279663207333158
1102	24-25	0.24613655688364133
1102	26-27	0.26831288863963465
1102	28-29	0.28451892163752035
1102	30-31	0.2376901073434894
1102	32-33	0.2802395212216524
1102	34-35	0.21574322383189326
1102	36-37	0.21108760455961573
1102	38-39	0.19857194829901914
1102	40-41	0.26843158106703413
1102	42-43	0.21939854405103176
1102	44-45	0.20299975396936532
1102	46-47	0.171357755473025
1102	48-49	0.17709033701633814
1102	50-51	0.17070642095080757
1102	52-53	0.19133581116860654
1102	54-55	0.13483054079012646
1102	56-57	0.2072459948138885
1102	58-59	0.21325572273200066
1102	60-61	0.20158975662496204
1102	62-63	0.1555245024189702
1102	64-65	0.21589795539328094
1102	66-67	0.17323657222068078
1102	68-69	0.11201881485333587
1102	70-71	0.11989794087929795
1102	72-73	0.08566551822432444
1102	74-75	0.08320326513994303
1102	76-77	0.1014280376473593
1102	78-79	0.1117233200393386
1102	80-81	0.1146582651511352
1102	82-83	0.128173902097501
1102	84-85	0.1486962614629519
1102	86-87	0.17027726046577385
1102	88-89	0.20382092807385277
1102	90-91	0.13202963013699787
1102	92-93	0.17076631590277458
1102	94-95	0.17715418499717117
1102	96-97	0.16414918847657844
1102	98-99	0.10885671561614885
1102	100	0.12328037307644735
1103	1	0.09577112306308067
1103	2	0.0747129535103781
1103	3	0.10003050778166767
1103	4	0.0802745577078241
1103	5	0.11481046098854364
1103	6	0.12277928751912981
1103	7	0.14827404631693497
1103	8	0.14776703331028784
1103	9	0.1653009478718772
1103	10-11	0.19719894854813447
1103	12-13	0.177591139984834
1103	14-15	0.24372993804247756
1103	16-17	0.2619548125225606
1103	18-19	0.21953351444347646
1103	20-21	0.2647444910570371
1103	22-23	0.2640259966207452
1103	24-25	0.2501322910337791
1103	26-27	0.25508938943769977
1103	28-29	0.2813966788565452
1103	30-31	0.2512435361819101
1103	32-33	0.20502670526777678
1103	34-35	0.2752432955866837
1103	36-37	0.2771266523132567
1103	38-39	0.2563451834323516
1103	40-41	0.2527499160052571
1103	42-43	0.2886573772852188
1103	44-45	0.32241068545613416
1103	46-47	0.3059985926049791
1103	48-49	0.27209017816984726
1103	50-51	0.2709293428279125
1103	52-53	0.22619258093212835
1103	54-55	0.25385132338843874
1103	56-57	0.22957897868494115
1103	58-59	0.29673272997649747
1103	60-61	0.3250235138045525
1103	62-63	0.2530248392830856
1103	64-65	0.22998411153828613
1103	66-67	0.252750096629903
1103	68-69	0.24623853350880864
1103	70-71	0.298729060768963
1103	72-73	0.29419933225999984
1103	74-75	0.28734462317883214
1103	76-77	0.29435615906304236
1103	78-79	0.2718878959575548
1103	80-81	0.24001969568975312
1103	82-83	0.2691060790283899
1103	84-85	0.27607010174664026
1103	86-87	0.27393404907176944
1103	88-89	0.2427283052334701
1103	90-91	0.26464928262470266
1103	92-93	0.30083807069637913
1103	94-95	0.3300620397917484
1103	96-97	0.36653667312598515
1103	98-99	0.33912586729920946
1103	100	0.3432180176130615
1104	1	0.09001208051989806
1104	2	0.10532025027139014
1104	3	0.11818020240831117
1104	4	0.08264114373612585
1104	5	0.1146450303817943
1104	6	0.09962891335574398
1104	7	0.13426061867086503
1104	8	0.11781664994701657
1104	9	0.11356465830976958
1104	10-11	0.18017841399316836
1104	12-13	0.19545312920842406
1104	14-15	0.21385767004154133
1104	16-17	0.23209561253594302
1104	18-19	0.18848980873097076
1104	20-21	0.2347915986350202
1104	22-23	0.21798481042139883
1104	24-25	0.24291611262297863
1104	26-27	0.24712101612321646
1104	28-29	0.2386806018535239
1104	30-31	0.20902301919034727
1104	32-33	0.22801256881260912
1104	34-35	0.24649194958121257
1104	36-37	0.22827176581679964
1104	38-39	0.23080317447083587
1104	40-41	0.2219114303894827
1104	42-43	0.23304091200536448
1104	44-45	0.2179329564888448
1104	46-47	0.2091263225131712
1104	48-49	0.20038316095980235
1104	50-51	0.2313774189025679
1104	52-53	0.2557939751324412
1104	54-55	0.19621088405225606
1104	56-57	0.256131761962898
1104	58-59	0.22569280876093956
1104	60-61	0.2522798998817919
1104	62-63	0.263621305273837
1104	64-65	0.21528895172169626
1104	66-67	0.2354331586015661
1104	68-69	0.2577317161871022
1104	70-71	0.24828102651194683
1104	72-73	0.21248104519440147
1104	74-75	0.2365781684579389
1104	76-77	0.211447679071739
1104	78-79	0.20312383738775708
1104	80-81	0.23137055201060264
1104	82-83	0.21609474853726596
1104	84-85	0.224797946235185
1104	86-87	0.21575909658707815
1104	88-89	0.18979705553404358
1104	90-91	0.23643165523246523
1104	92-93	0.2514170150935442
1104	94-95	0.21560722689435607
1104	96-97	0.1971396835783068
1104	98-99	0.21155750067554635
1104	100	0.15698564113715463
1105	1	0.03618494860756982
1105	2	0.07106651553177556
1105	3	0.04610520286481545
1105	4	0.05158833468554036
1105	5	0.06387941460371138
1105	6	0.05330840060029374
1105	7	0.07193365943219732
1105	8	0.06876608335645784
1105	9	0.11850850311022043
1105	10-11	0.11747741839957371
1105	12-13	0.12582496913365304
1105	14-15	0.12077605000262537
1105	16-17	0.1406299985679098
1105	18-19	0.12474266210173113
1105	20-21	0.15606125127667525
1105	22-23	0.16712350790462693
1105	24-25	0.1512677658528787
1105	26-27	0.1260276852022102
1105	28-29	0.10263434752933875
1105	30-31	0.1234046251364802
1105	32-33	0.155834950011716
1105	34-35	0.1657032035643624
1105	36-37	0.15966443410489006
1105	38-39	0.20021584309535712
1105	40-41	0.13539725302912586
1105	42-43	0.14381952615612192
1105	44-45	0.14556196636654306
1105	46-47	0.18167856303728058
1105	48-49	0.20232110960314031
1105	50-51	0.22561392863073593
1105	52-53	0.21558128106337193
1105	54-55	0.2508648436851786
1105	56-57	0.20714854269380822
1105	58-59	0.24138422521196645
1105	60-61	0.23171108692198317
1105	62-63	0.2254937010592002
1105	64-65	0.2268810919476536
1105	66-67	0.2714265859345275
1105	68-69	0.25984592461794165
1105	70-71	0.1963889263907106
1105	72-73	0.2060983031942598
1105	74-75	0.20612583140199092
1105	76-77	0.1850121871971524
1105	78-79	0.21379972639537215
1105	80-81	0.2275904713224719
1105	82-83	0.2220152693480273
1105	84-85	0.19833397509427186
1105	86-87	0.1548780669590073
1105	88-89	0.2168772616587944
1105	90-91	0.19401589862037838
1105	92-93	0.22538965278185685
1105	94-95	0.16242381031019448
1105	96-97	0.1967470273172367
1105	98-99	0.175937280592251
1105	100	0.19036128347896408
1106	1	0.00931707369659307
1106	2	0.02686442984919779
1106	3	0.049977057063230745
1106	4	0.0464405126973233
1106	5	0.02249665431829584
1106	6	0.045008032616152605
1106	7	0.06415904035242903
1106	8	0.0701680995272369
1106	9	0.05548503814065242
1106	10-11	0.08366436743192907
1106	12-13	0.07106066108637776
1106	14-15	0.12316787626343029
1106	16-17	0.15681553094017886
1106	18-19	0.14835426171449484
1106	20-21	0.13202068240860854
1106	22-23	0.14929509850880152
1106	24-25	0.15844466702311877
1106	26-27	0.16562821264270156
1106	28-29	0.14522269127309784
1106	30-31	0.13788367397486212
1106	32-33	0.15298376885514386
1106	34-35	0.1516376400011339
1106	36-37	0.10017392272992964
1106	38-39	0.1319554707474495
1106	40-41	0.13022989946051666
1106	42-43	0.14612896847814483
1106	44-45	0.1369701616191179
1106	46-47	0.13639001171655707
1106	48-49	0.10910435110240257
1106	50-51	0.1147842286232077
1106	52-53	0.09996038701491727
1106	54-55	0.1394070210352112
1106	56-57	0.01724886704787565
1106	58-59	0.059107020880290406
1106	60-61	0.045694635030784525
1106	62-63	0.009761667927072892
1106	64-65	0.06918400923260748
1106	66-67	0.11674157648548089
1106	68-69	0.10309746956976795
1106	70-71	0.0526153975664343
1106	72-73	0.045758834399030945
1106	74-75	0.06323053636687348
1106	76-77	0.041526028443470864
1106	78-79	0.06081972081503295
1106	80-81	0.06506690216538402
1106	82-83	0.08841705430918267
1106	84-85	0.06211988384754363
1106	86-87	0.020646020633989792
1106	88-89	0.08223745779481817
1106	90-91	0.06443292210909135
1106	92-93	0.08934071126177656
1106	94-95	0.1394504237017884
1106	96-97	0.10297084082336738
1106	98-99	0.12183337098081637
1106	100	0.1259815096733803
1107	1	0.02191723523876732
1107	2	0.034659708333897754
1107	3	0.043612562401854404
1107	4	0.04833535082690332
1107	5	0.06725530200878183
1107	6	0.062317146692464576
1107	7	0.07945407059906984
1107	8	0.01800941588565763
1107	9	0.04827653408079158
1107	10-11	0.05744294018104057
1107	12-13	0.07734051708452938
1107	14-15	0.10794747286881545
1107	16-17	0.08260687089720875
1107	18-19	0.1131779322328228
1107	20-21	0.09371430416256743
1107	22-23	0.13103392976196915
1107	24-25	0.14548518660593857
1107	26-27	0.12562354777871576
1107	28-29	0.08784470873624883
1107	30-31	0.11882049057731336
1107	32-33	0.09276524804676001
1107	34-35	0.10723140806468479
1107	36-37	0.06939142516796437
1107	38-39	0.11989320404779846
1107	40-41	0.08200935946639731
1107	42-43	0.106387289664724
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2216	30-31	-0.30826912441187204
2216	32-33	-0.34573234012148646
2216	34-35	-0.3742046356309743
2216	36-37	-0.37206078743333393
2216	38-39	-0.38072881267208203
2216	40-41	-0.3892027862986325
2216	42-43	-0.4152867862499079
2216	44-45	-0.39367564661147014
2216	46-47	-0.3121887881178651
2216	48-49	-0.3353145476648436
2216	50-51	-0.4049258128964084
2216	52-53	-0.38864441515263337
2216	54-55	-0.3507426916035925
2216	56-57	-0.4018704478009809
2216	58-59	-0.38263610941989157
2216	60-61	-0.391599934362759
2216	62-63	-0.40577245349437874
2216	64-65	-0.44724728122540824
2216	66-67	-0.4348641635074202
2216	68-69	-0.4577420280037714
2216	70-71	-0.4505307814408894
2216	72-73	-0.4409129112326369
2216	74-75	-0.4880491635880446
2216	76-77	-0.4466844296004595
2216	78-79	-0.5172637360490597
2216	80-81	-0.49094140766082717
2216	82-83	-0.44868603933390006
2216	84-85	-0.5289097318063938
2216	86-87	-0.547117557357879
2216	88-89	-0.6245705713478671
2216	90-91	-0.5934472015274252
2216	92-93	-0.5791955728395379
2216	94-95	-0.4899865564267927
2216	96-97	-0.5725691171036189
2216	98-99	-0.6364501061007566
2216	100	-0.6748294768288723
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26.0
3	15.0
4	13.0
5	42.0
6	95.0
7	245.0
8	569.0
9	1108.0
10	2053.0
11	3189.0
12	4988.0
13	7515.0
14	9100.0
15	12086.0
16	14175.0
17	19441.0
18	23157.0
19	12380.0
20	8750.0
21	8199.0
22	8503.0
23	9451.0
24	10937.0
25	13565.0
26	17535.0
27	22916.0
28	29556.0
29	37882.0
30	49351.0
31	63321.0
32	81484.0
33	105544.0
34	146288.0
35	225312.0
36	417862.0
37	957421.0
38	1767401.0
39	848683.0
40	2397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4889916082393	19.770492692581577	13.58739369620037	24.15312200297875
2	15.540220150008984	20.557075980836554	39.931201601090976	23.97150226806348
3	17.910897116966883	25.581544509053195	30.380104884287974	26.12745348969195
4	12.240860857288189	17.044173888834262	39.12933780239729	31.585627451480264
5	12.583021567246483	37.851906539571445	36.08773301020669	13.477338882975376
6	30.615337987339338	38.47020729372195	17.72758283574963	13.186871883189088
7	26.765063818207384	32.63409309557506	22.99371478921327	17.60712829700428
8	25.00092563798713	35.876878615172735	20.78347617832704	18.338719568513092
9	25.29778626641484	16.01131398638963	21.245125243927482	37.44577450326805
10-11	23.801675014628152	26.419173685347808	29.29488051044365	20.484270789580382
12-13	24.849991552951863	23.879946707725054	29.28008894185295	21.989972797470134
14-15	21.971343467667825	25.647460879410538	26.72733118086295	25.653864472058686
16-17	21.217867541626802	29.031528063758195	27.1816222762789	22.568982118336105
18-19	21.024334727694853	27.79174612166858	29.72907572546421	21.454843425172356
20-21	22.291653262665413	27.054524846870663	29.29254794181308	21.36127394865084
22-23	21.84518334110766	26.905436151929287	29.296843663376563	21.952536843586493
24-25	21.765386772502616	27.044262287121555	29.292611905087558	21.89773903528827
26-27	21.508808358924885	27.000417497083234	29.403043914886435	22.087730229105446
28-29	21.559452605562075	27.0575849431325	29.436279954727752	21.946682496577672
30-31	21.83515466111811	27.425854033182883	28.99593489026308	21.743056415435923
32-33	21.550635248368504	27.128094679775945	29.421807142257393	21.899462929598155
34-35	21.70985451856378	26.949563535458886	29.453005581121506	21.88757636485583
36-37	22.077184775890203	27.295801463008505	28.761551464778844	21.86546229632245
38-39	21.895264696093417	27.4842262756813	28.88296640098087	21.73754262724441
40-41	21.768851037105723	27.22887341825166	29.03087736236718	21.971398182275436
42-43	21.783025176249936	27.115307770980802	28.876481900555483	22.225185152213783
44-45	21.93448530163043	27.276550286238592	28.746275964556794	22.04268844757418
46-47	21.983296088763808	27.52996173031964	28.454240772232176	22.032501408684375
48-49	22.026805973833373	27.485409874042876	28.227961044439564	22.259823107684184
50-51	22.100006980195467	27.44176847804425	28.057330672091663	22.40089386966862
52-53	22.10486276834552	27.421192075758388	28.09584314185679	22.378102014039296
54-55	22.1734001948385	27.75194206235439	27.948864504289784	22.125793238517325
56-57	21.965430834861728	27.490670311205438	28.061660416525463	22.48223843740737
58-59	21.820374280104115	27.101114706867197	28.46526745782293	22.61324355520576
60-61	22.106592642873977	27.55280416707553	27.99845424077223	22.342148949278258
62-63	21.95453566020004	27.50868730848721	28.01732100098026	22.519456030332492
64-65	21.973817185645885	27.205767057726217	28.204289077208045	22.616126679419853
66-67	21.855085260061372	27.29215226660627	28.03230596647948	22.820456506852885
68-69	22.261142791820813	27.469117630694306	27.862791335233165	22.406948242251715
70-71	22.180686120911766	27.30965333816754	27.966843865859243	22.54281667506145
72-73	22.065743942536418	27.46021832098846	27.944149757847132	22.52988797862799
74-75	22.164742895873587	27.39119482858039	27.81568218834407	22.62838008720195
76-77	22.102485455397055	27.44976029604122	27.7396811972755	22.708073051286227
78-79	22.250283507214384	27.587644447052185	27.744628031453367	22.417444014280065
80-81	22.146733824914442	27.392047230632738	27.810626285392882	22.65059265905994
82-83	21.99131015366562	27.354918007910893	28.005280674955234	22.64849116346825
84-85	21.962567943098254	27.324066196531955	27.838496486129138	22.87486937424065
86-87	22.08584140965776	27.50014011034423	27.75312779904555	22.66089068095246
88-89	21.799395681392095	27.380065983790146	27.828873917383888	22.991664417433867
90-91	21.780958130004688	27.24268499935103	27.897211280403695	23.07914559024059
92-93	21.894955652195378	27.48366117540802	27.675941553725004	22.9454416186716
94-95	21.659640236809942	27.197179824336892	27.8912779267456	23.251902012107568
96-97	21.621988267178143	27.220794145526245	27.854081247616357	23.303136339679252
98-99	21.60447898216134	27.32162730120263	27.73685817942413	23.337035537211904
100	21.7194143514842	27.23498271642905	27.628443183737968	23.41715974834878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	30.0
2	122.0
3	206.0
4	130.5
5	99.0
6	257.0
7	581.5
8	976.0
9	1192.5
10	1016.5
11	795.0
12	883.0
13	1125.0
14	1408.5
15	1682.5
16	1972.0
17	2334.5
18	2762.5
19	3297.5
20	3933.0
21	4799.5
22	6251.5
23	8569.5
24	11959.5
25	16365.0
26	22294.0
27	29994.5
28	40419.5
29	53249.0
30	67029.0
31	82573.5
32	99067.5
33	116254.5
34	132949.5
35	145522.0
36	155412.5
37	165078.5
38	173534.5
39	178618.0
40	182003.0
41	185814.5
42	185819.5
43	184546.5
44	182719.0
45	178667.0
46	175351.5
47	170388.0
48	165596.5
49	162067.0
50	156850.0
51	151627.0
52	144104.0
53	136263.0
54	130380.5
55	123344.5
56	113363.5
57	102912.0
58	93776.5
59	84865.0
60	75791.0
61	64857.0
62	54376.0
63	45475.5
64	36680.0
65	29132.0
66	23077.0
67	17472.0
68	13613.0
69	10573.5
70	7615.5
71	5497.5
72	4064.0
73	2977.5
74	2102.0
75	1404.0
76	935.0
77	618.5
78	424.0
79	280.5
80	203.5
81	110.5
82	30.5
83	8.5
84	2.5
85	2.5
86	1.5
87	1.5
88	3.0
89	3.5
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04517906224614597
2	0.2411101141009053
3	0.0031562622975363957
4	0.0026909159331560298
5	2.8325430875326626E-4
6	6.069735187569992E-5
7	0.0
8	3.6418411125419946E-4
9	0.0
10-11	6.272059693822325E-4
12-13	0.0
14-15	3.439516606289662E-4
16-17	1.2139470375139984E-4
18-19	6.069735187569992E-4
20-21	2.0232450625233307E-5
22-23	1.1127847843878318E-4
24-25	0.0012746443893896983
26-27	1.1127847843878318E-4
28-29	2.6302185812803293E-4
30-31	1.4162715437663313E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	4.0464901250466615E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.0116225312616654E-5
68-69	2.0232450625233306E-4
70-71	1.0116225312616654E-5
72-73	3.237192100037329E-4
74-75	1.0116225312616653E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.0116225312616653E-4
84-85	0.0
86-87	4.451139137551327E-4
88-89	0.0026099861306550965
90-91	0.00253917255346678
92-93	6.676708706326992E-4
94-95	5.260437162560659E-4
96-97	2.0232450625233306E-4
98-99	7.081357718831657E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4942555.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.0027270532965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.56499640836931	34.45999070173019
2	16.375654634243997	16.70406087258793
3	6.617691602718	10.125609556089554
4	3.2229690153222093	6.575208359588418
5	1.7801430335980373	4.5396074629213965
6	1.1605412767984014	3.551446198477985
7	0.7372453868270991	2.6321067664951174
8	0.5111691250099024	2.0856815488761957
9	0.3806464708380628	1.7472607250358874
>10	1.5628073485465213	13.279781826667758
>50	0.06518732244272478	2.255105859602089
>100	0.02027365170022358	1.5593261107470808
>500	5.159655208743782E-4	0.17062651178730795
>1k	7.937903237298531E-5	0.08093045696626415
>5k	7.937903237298443E-5	0.23325704242677697
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6315	0.12776792569834833	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5208	0.10537060285621506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.046490125046661E-5	0.0
5	0.0	4.046490125046661E-5	0.0	4.046490125046661E-5	0.0
6	0.0	6.0697351875699915E-5	0.0	4.046490125046661E-5	0.0
7	0.0	6.0697351875699915E-5	0.0	8.092980250093322E-5	0.0
8	0.0	6.0697351875699915E-5	0.0	1.2139470375139983E-4	0.0
9	0.0	6.0697351875699915E-5	0.0	1.2139470375139983E-4	0.0
10-11	0.0	6.0697351875699915E-5	0.0	1.2139470375139983E-4	0.0
12-13	0.0	7.081357718831657E-5	0.0	1.719758303144831E-4	0.0
14-15	0.0	8.092980250093322E-5	0.0	4.046490125046661E-4	0.0
16-17	0.0	8.092980250093322E-5	0.0	4.552301390677494E-4	0.0
18-19	0.0	8.092980250093322E-5	0.0	4.855788150055993E-4	0.0
20-21	0.0	8.092980250093322E-5	0.0	5.058112656308327E-4	0.0
22-23	0.0	8.092980250093322E-5	0.0	5.665086175065325E-4	0.0
24-25	0.0	8.092980250093322E-5	0.0	6.170897440696158E-4	0.0
26-27	0.0	8.092980250093322E-5	0.0	7.486006731336323E-4	0.0
28-29	0.0	8.092980250093322E-5	0.0	0.001254411938764465	0.0
30-31	0.0	8.092980250093322E-5	0.0	0.004289279532549461	0.0
32-33	0.0	8.092980250093322E-5	0.0	0.012210283952328298	0.0
34-35	0.0	8.092980250093322E-5	0.0	0.02327743444433092	0.0
36-37	0.0	8.092980250093322E-5	0.0	0.044986853965206255	0.0
38-39	0.0	8.092980250093322E-5	0.0	0.08099049985280893	0.0
40-41	0.0	1.0116225312616653E-4	0.0	0.12139470375139982	0.0
42-43	0.0	1.0116225312616653E-4	0.0	0.1650563322006533	0.0
44-45	0.0	1.0116225312616653E-4	0.0	0.21757977402375897	0.0
46-47	0.0	1.0116225312616653E-4	0.0	0.2829407057685751	0.0
48-49	0.0	1.0116225312616653E-4	0.0	0.35564601709035104	0.0
50-51	0.0	1.0116225312616653E-4	0.0	0.4350685020197044	0.0
52-53	0.0	1.0116225312616653E-4	0.0	0.516069118097826	0.0
54-55	0.0	1.0116225312616653E-4	0.0	0.6038880700366511	0.0
56-57	0.0	1.0116225312616653E-4	0.0	0.7108368040416344	0.0
58-59	0.0	1.0116225312616653E-4	0.0	0.8315537206970889	0.0
60-61	0.0	1.0116225312616653E-4	0.0	0.9600500146179456	0.0
62-63	0.0	1.0116225312616653E-4	0.0	1.0930783774788546	0.0
64-65	0.0	1.0116225312616653E-4	0.0	1.2353428540501827	0.0
66-67	0.0	1.0116225312616653E-4	0.0	1.3971417617001733	0.0
68-69	0.0	1.0116225312616653E-4	0.0	1.5842919299835814	0.0
70-71	0.0	1.0116225312616653E-4	0.0	1.7879113130759294	0.0
72-73	0.0	1.0116225312616653E-4	0.0	2.002800171166532	0.0
74-75	0.0	1.0116225312616653E-4	0.0	2.222210981971875	0.0
76-77	0.0	1.0116225312616653E-4	0.0	2.467559794478767	0.0
78-79	0.0	1.0116225312616653E-4	0.0	2.74280205278444	0.0
80-81	0.0	1.0116225312616653E-4	0.0	3.0433449905969683	0.0
82-83	0.0	1.0116225312616653E-4	0.0	3.357342508075277	0.0
84-85	0.0	1.0116225312616653E-4	0.0	3.6761047676758274	0.0
86-87	0.0	1.0116225312616653E-4	0.0	4.019914801150417	0.0
88	0.0	1.0116225312616653E-4	0.0	4.297433210151429	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6210	0.0	48.200764	1
GTATCAA	11535	0.0	44.093613	1
TCAACGC	13285	0.0	37.641514	4
ATCAACG	13640	0.0	36.765953	3
CAACGCA	13715	0.0	36.494884	5
AACGCAG	14165	0.0	35.36868	6
TATCAAC	14575	0.0	34.46265	2
ACGCAGA	16380	0.0	30.356382	7
CGCAGAG	16720	0.0	29.682869	8
GTACATG	12940	0.0	25.827583	1
TACATGG	12590	0.0	25.262602	2
GCAGAGT	19140	0.0	25.24233	9
ACATGGG	13250	0.0	23.19838	3
AGAGTAC	17260	0.0	21.11637	10-11
GAGTACT	12785	0.0	20.309584	12-13
CAGAGTA	18660	0.0	20.17433	10-11
GTGGTAT	3105	0.0	19.280306	1
CATGGGG	9890	0.0	19.198713	4
AGTACTT	13675	0.0	18.626934	12-13
GTACTTT	14350	0.0	18.078259	14-15
>>END_MODULE