FastQCFastQC Report
Wed 25 May 2016
SRR1033031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4517039
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38450.037.7825741
GTATCAA61000.036.5323751
TCAACGC60800.035.2602844
ATCAACG63250.033.8201453
CAACGCA64350.033.1685945
AACGCAG69200.030.9043166
TATCAAC75000.028.8347592
GTACATG139300.028.4177931
TACATGG139050.027.4539452
ACATGGG146100.025.4536953
ACGCAGA83300.025.1656687
CGCAGAG87900.023.4411838
CATGGGG114250.022.5501234
GAGTACA99200.019.6682531
GCAGAGT101600.019.1257329
AGTACAT103100.017.5558472
GTATAGA14150.016.2805461
TATACTG23300.016.1418695
CAGAGTA94800.015.93598510-11
ATGGGGG63100.015.422715
Produced by FastQC (version 0.11.5)