FastQCFastQC Report
Wed 25 May 2016
SRR1033031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4517039
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66600.14744172011797993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53460.11835186722983794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.051.869771
GTATCAA108000.047.317531
TCAACGC130900.038.52634
ATCAACG133350.037.8201453
CAACGCA136350.036.984745
TATCAAC139700.036.4232562
AACGCAG142400.035.4464156
ACGCAGA161700.030.895937
CGCAGAG164800.030.0584248
GTACATG136950.027.5043431
TACATGG138500.026.295482
GCAGAGT188000.025.9238179
ACATGGG144400.024.6077023
AGAGTAC169750.021.29092810-11
CATGGGG110900.021.1478524
GAGTACT117300.021.05476212-13
CAGAGTA179500.020.80211410-11
TACACCG5850.020.0845535
TATACGC1508.298103E-418.7991435
AGTACTT128300.018.77339712-13
Produced by FastQC (version 0.11.5)