##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033031_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4517039
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.729428946706015	34.0	31.0	34.0	31.0	34.0
2	32.730590991133795	34.0	31.0	34.0	31.0	34.0
3	32.83870340725418	34.0	31.0	34.0	31.0	34.0
4	36.31877586179796	37.0	37.0	37.0	35.0	37.0
5	36.30276271690371	37.0	37.0	37.0	35.0	37.0
6	36.36558727963163	37.0	37.0	37.0	35.0	37.0
7	36.31587993816303	37.0	37.0	37.0	35.0	37.0
8	36.325875645527965	37.0	37.0	37.0	35.0	37.0
9	38.13635215458623	39.0	39.0	39.0	37.0	39.0
10-11	38.09726725848504	39.0	39.0	39.0	36.0	39.0
12-13	38.07188359454058	39.0	39.0	39.0	35.0	39.0
14-15	39.57565741628531	41.0	40.0	41.0	37.0	41.0
16-17	39.57154675883914	41.0	40.0	41.0	37.0	41.0
18-19	39.581581429781764	41.0	40.0	41.0	37.0	41.0
20-21	39.57164937030652	41.0	40.0	41.0	37.0	41.0
22-23	39.50312184597034	41.0	39.5	41.0	36.5	41.0
24-25	39.44407033014326	41.0	39.0	41.0	36.0	41.0
26-27	39.3356416670301	41.0	39.0	41.0	36.0	41.0
28-29	39.28437002647088	41.0	39.0	41.0	36.0	41.0
30-31	39.28223986996792	41.0	39.0	41.0	36.0	41.0
32-33	39.248948813592264	41.0	39.0	41.0	35.5	41.0
34-35	39.169749918032586	41.0	39.0	41.0	35.0	41.0
36-37	39.082374869909245	41.0	39.0	41.0	35.0	41.0
38-39	38.99142258900133	41.0	39.0	41.0	35.0	41.0
40-41	38.89025078154074	41.0	39.0	41.0	35.0	41.0
42-43	38.77250063592544	41.0	39.0	41.0	35.0	41.0
44-45	38.60769167589653	41.0	38.0	41.0	35.0	41.0
46-47	38.41682327737263	41.0	38.0	41.0	34.5	41.0
48-49	38.22381055377206	40.0	38.0	41.0	34.0	41.0
50-51	38.077276950674985	40.0	38.0	41.0	34.0	41.0
52-53	37.9343446005226	40.0	37.5	41.0	34.0	41.0
54-55	37.773228325015566	40.0	37.0	41.0	33.5	41.0
56-57	37.606611875611435	40.0	37.0	41.0	33.0	41.0
58-59	37.412774718128404	40.0	36.0	41.0	33.0	41.0
60-61	37.207251476022236	39.5	36.0	41.0	33.0	41.0
62-63	36.962191493144076	39.0	35.0	41.0	33.0	41.0
64-65	36.68388517345102	39.0	35.0	41.0	32.0	41.0
66-67	36.3944602869269	38.5	35.0	41.0	32.0	41.0
68-69	36.08403303580066	37.5	35.0	40.0	32.0	41.0
70-71	35.75420081606556	37.0	35.0	40.0	31.0	41.0
72-73	35.40837947159633	36.5	35.0	39.0	31.0	41.0
74-75	35.065455600449766	36.0	35.0	39.0	31.0	41.0
76-77	34.71506588718849	35.5	35.0	38.5	31.0	40.0
78-79	34.363753556256654	35.0	34.5	37.0	30.5	39.0
80-81	34.04971298675969	35.0	34.0	37.0	30.0	39.0
82-83	33.76514127949748	35.0	34.0	36.0	30.0	38.5
84-85	33.55968666642019	35.0	34.0	36.0	30.0	37.0
86-87	33.366753308970765	35.0	34.0	36.0	30.0	37.0
88-89	33.17459678785151	35.0	34.0	35.0	30.0	36.5
90-91	33.00358033216007	35.0	34.0	35.0	30.0	36.0
92-93	32.841860674658776	35.0	34.0	35.0	30.0	36.0
94-95	32.697494641954606	35.0	34.0	35.0	29.0	36.0
96-97	32.561641044055634	35.0	34.0	35.0	29.0	36.0
98-99	32.44442864008923	35.0	34.0	35.0	29.0	35.0
100	32.34694099386788	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11947112721761499
1101	2	0.18393697230519734
1101	3	0.20522522799848986
1101	4	0.14876215528835957
1101	5	0.11435120946622135
1101	6	0.1299804791216701
1101	7	0.17579757080807923
1101	8	0.19431149062892672
1101	9	0.23557315082165076
1101	10-11	0.2134306753652524
1101	12-13	0.23228920412456944
1101	14-15	0.32131680951837893
1101	16-17	0.31875229518163906
1101	18-19	0.2884006388123268
1101	20-21	0.25598659856500916
1101	22-23	0.3166720557095033
1101	24-25	0.3520217043303262
1101	26-27	0.3229830269039837
1101	28-29	0.3177903818798029
1101	30-31	0.3597687865591155
1101	32-33	0.3723293905101315
1101	34-35	0.3803551617733305
1101	36-37	0.34982528251884304
1101	38-39	0.34454236932792526
1101	40-41	0.3466853384177355
1101	42-43	0.35731041684630327
1101	44-45	0.3899186023940686
1101	46-47	0.3976481738427893
1101	48-49	0.4080170328137598
1101	50-51	0.3855042843358092
1101	52-53	0.3938070711393138
1101	54-55	0.427770443983988
1101	56-57	0.36630074589862005
1101	58-59	0.31885475617910686
1101	60-61	0.3435719563275441
1101	62-63	0.3225288916928406
1101	64-65	0.32830544460584576
1101	66-67	0.36673968021198533
1101	68-69	0.3464638264205533
1101	70-71	0.30715496479599835
1101	72-73	0.2877200664551438
1101	74-75	0.28817415243419475
1101	76-77	0.24902369612952668
1101	78-79	0.28287077346889333
1101	80-81	0.2668041813768198
1101	82-83	0.3141404924914184
1101	84-85	0.3331656043682685
1101	86-87	0.3785291875552659
1101	88-89	0.370462412048596
1101	90-91	0.3856697394536468
1101	92-93	0.39635827302934246
1101	94-95	0.4488480259481733
1101	96-97	0.4778910785334318
1101	98-99	0.4946376490417279
1101	100	0.5080439842983253
1102	1	0.10922600530080473
1102	2	0.07920557577351417
1102	3	0.12987916120468412
1102	4	0.09368076578247297
1102	5	0.12017207474222857
1102	6	0.12622477048387282
1102	7	0.16689496031852258
1102	8	0.15051603849968131
1102	9	0.24657617519190467
1102	10-11	0.1964947292526844
1102	12-13	0.20211631537641495
1102	14-15	0.2926269053741777
1102	16-17	0.2942195961556422
1102	18-19	0.28203238845797074
1102	20-21	0.2679391885472171
1102	22-23	0.27162641083401695
1102	24-25	0.35385649179926304
1102	26-27	0.3324594012737663
1102	28-29	0.3427880090702331
1102	30-31	0.3236774551948969
1102	32-33	0.2962326893282352
1102	34-35	0.3190457670720619
1102	36-37	0.31891508416905623
1102	38-39	0.34536135203249785
1102	40-41	0.3733596370280594
1102	42-43	0.33132356320889045
1102	44-45	0.3262783961406299
1102	46-47	0.24351181809635847
1102	48-49	0.2513277619888328
1102	50-51	0.297871025782797
1102	52-53	0.23848998254957365
1102	54-55	0.24190275526581928
1102	56-57	0.22672707066028863
1102	58-59	0.26057318049674194
1102	60-61	0.21767120788782535
1102	62-63	0.2860268148788023
1102	64-65	0.2955329256454533
1102	66-67	0.2571734383518631
1102	68-69	0.28748531452700377
1102	70-71	0.2409901604166933
1102	72-73	0.23888069098432396
1102	74-75	0.22444929459612695
1102	76-77	0.2030987151048791
1102	78-79	0.17827159872208398
1102	80-81	0.1321973309829545
1102	82-83	0.16173975074971736
1102	84-85	0.17157125875208834
1102	86-87	0.18558777585756303
1102	88-89	0.1977640017027511
1102	90-91	0.16788429901315993
1102	92-93	0.1987296147341553
1102	94-95	0.20852371632174993
1102	96-97	0.24175569699277588
1102	98-99	0.14904614395649673
1102	100	0.18775185937538197
1103	1	0.1183772599022177
1103	2	0.10057056092053074
1103	3	0.10582985496279207
1103	4	0.08045961347737318
1103	5	0.08898379310137727
1103	6	0.08631020150770041
1103	7	0.13695078139753747
1103	8	0.14197427283516362
1103	9	0.16940470581042888
1103	10-11	0.180480422439679
1103	12-13	0.15416381437530902
1103	14-15	0.21406323035285624
1103	16-17	0.24762840623611737
1103	18-19	0.21631233833158348
1103	20-21	0.23204744569829927
1103	22-23	0.19706166469221387
1103	24-25	0.2196425512525053
1103	26-27	0.18613090648746322
1103	28-29	0.27908152030457245
1103	30-31	0.2740766988197336
1103	32-33	0.23272719933112285
1103	34-35	0.26054389971766057
1103	36-37	0.2695878094245572
1103	38-39	0.2640057910215603
1103	40-41	0.22687415969376445
1103	42-43	0.26149726482790925
1103	44-45	0.2838343335004865
1103	46-47	0.26501683129426823
1103	48-49	0.24057773786653058
1103	50-51	0.31403449943434225
1103	52-53	0.30567293434034326
1103	54-55	0.35523440233369996
1103	56-57	0.26583539396528266
1103	58-59	0.24511200221675722
1103	60-61	0.3044838800090517
1103	62-63	0.2975689672913475
1103	64-65	0.2753442252298939
1103	66-67	0.21670445868441135
1103	68-69	0.2507119787486616
1103	70-71	0.29030978313152644
1103	72-73	0.333974959761413
1103	74-75	0.275695537429101
1103	76-77	0.28027439924066044
1103	78-79	0.3023962494942296
1103	80-81	0.21399675063447177
1103	82-83	0.25298596248353533
1103	84-85	0.2611766352618261
1103	86-87	0.31847724691298396
1103	88-89	0.29876138170780564
1103	90-91	0.2738729165576501
1103	92-93	0.3597727306886469
1103	94-95	0.36731493951738514
1103	96-97	0.3661327965439938
1103	98-99	0.34028512377208386
1103	100	0.33879637779278227
1104	1	0.09119239165793402
1104	2	0.04245864469250904
1104	3	0.07586587787030652
1104	4	0.04766477354509391
1104	5	0.10230638196328101
1104	6	0.09508269784213752
1104	7	0.12241050654774455
1104	8	0.10420092223526467
1104	9	0.12035734670131859
1104	10-11	0.14675612704119345
1104	12-13	0.13710107126884452
1104	14-15	0.17880328521832212
1104	16-17	0.18288631808538014
1104	18-19	0.18907045759932117
1104	20-21	0.20716225425514523
1104	22-23	0.22812029194108163
1104	24-25	0.2450776385943172
1104	26-27	0.22314032090987723
1104	28-29	0.1899923731581623
1104	30-31	0.21878688127085866
1104	32-33	0.23526970180982687
1104	34-35	0.2036609784907455
1104	36-37	0.19702496084953935
1104	38-39	0.23756000769035523
1104	40-41	0.24435164884977212
1104	42-43	0.2098514034995702
1104	44-45	0.19901612413510605
1104	46-47	0.15226837155979212
1104	48-49	0.13491935425352608
1104	50-51	0.15798107550010343
1104	52-53	0.14539474352174153
1104	54-55	0.15779866330188952
1104	56-57	0.20891240429428848
1104	58-59	0.18989837443774604
1104	60-61	0.22877811253955826
1104	62-63	0.20093648760284566
1104	64-65	0.23013297169446645
1104	66-67	0.2330216378908787
1104	68-69	0.24374945687039684
1104	70-71	0.2721390943123865
1104	72-73	0.25951762425373914
1104	74-75	0.24143871864595212
1104	76-77	0.19910818315572243
1104	78-79	0.15224411794778092
1104	80-81	0.20152805069812274
1104	82-83	0.19768081089952005
1104	84-85	0.19683458235470397
1104	86-87	0.2272859476704454
1104	88-89	0.20489594878566209
1104	90-91	0.2098511255563693
1104	92-93	0.19809520853784335
1104	94-95	0.22660056252598793
1104	96-97	0.1930045025017506
1104	98-99	0.18989081574515865
1104	100	0.15643003520270327
1105	1	0.05436971564434856
1105	2	0.09907985427386734
1105	3	0.09941831942556689
1105	4	0.06851127914396926
1105	5	0.04199971955998194
1105	6	0.07429034591612549
1105	7	0.09187386580345702
1105	8	0.10189754499069181
1105	9	0.13080787325963428
1105	10-11	0.09673364095930737
1105	12-13	0.128520532572729
1105	14-15	0.17123558020420404
1105	16-17	0.18300692077637848
1105	18-19	0.16982703610134564
1105	20-21	0.11578361743600851
1105	22-23	0.14252943967796483
1105	24-25	0.15308110836782873
1105	26-27	0.17485893694876609
1105	28-29	0.16466812188807012
1105	30-31	0.16342371074070172
1105	32-33	0.16993951086644188
1105	34-35	0.14924933722338096
1105	36-37	0.18242772756068604
1105	38-39	0.1866801089675718
1105	40-41	0.18625599475586085
1105	42-43	0.1522048187378715
1105	44-45	0.2035154943525015
1105	46-47	0.13534581648119826
1105	48-49	0.08770825609826716
1105	50-51	0.12425698649411743
1105	52-53	0.1458898065529226
1105	54-55	0.1490863588735678
1105	56-57	0.09426285117286426
1105	58-59	0.1620925803393689
1105	60-61	0.14746661889018498
1105	62-63	0.1257114483229671
1105	64-65	0.07630222702976397
1105	66-67	0.0653732635964488
1105	68-69	0.07327305470257528
1105	70-71	0.0726914863908874
1105	72-73	0.1023284411052785
1105	74-75	0.05268023344233086
1105	76-77	0.12097957162881556
1105	78-79	0.14540733982547493
1105	80-81	0.12332372249043289
1105	82-83	0.12058354376462432
1105	84-85	0.15098813214026308
1105	86-87	0.1104767953058925
1105	88-89	0.10423721858590795
1105	90-91	0.10130026061144548
1105	92-93	0.1708127727647124
1105	94-95	0.16801695922428195
1105	96-97	0.144415648755313
1105	98-99	0.1522208070584128
1105	100	0.17245395931076501
1106	1	0.005089274974039881
1106	2	0.0345305822349431
1106	3	0.03842680192864378
1106	4	0.03717026073623941
1106	5	0.05999769802126309
1106	6	0.030548167276428728
1106	7	0.03174961308737778
1106	8	0.0552910233289694
1106	9	0.04172760355667293
1106	10-11	0.047141591010586126
1106	12-13	0.022370886574734072
1106	14-15	0.1180781652695373
1106	16-17	0.1311901425635682
1106	18-19	0.12875713568644898
1106	20-21	0.1434827474947724
1106	22-23	0.10456311088863401
1106	24-25	0.10426765105485458
1106	26-27	0.1306774588143611
1106	28-29	0.11452004406046257
1106	30-31	0.11331973625034664
1106	32-33	0.11830712882307637
1106	34-35	0.1205065290347278
1106	36-37	0.13928885697384885
1106	38-39	0.09985535840743864
1106	40-41	0.10064761191003413
1106	42-43	0.10838402211756204
1106	44-45	0.11141707851178495
1106	46-47	0.17211494330727106
1106	48-49	0.14954411395007128
1106	50-51	0.0829249330497106
1106	52-53	0.13339685552298164
1106	54-55	0.13636357010900468
1106	56-57	0.09890736314674342
1106	58-59	0.13908357837112106
1106	60-61	0.13614800358136137
1106	62-63	0.08253109267086955
1106	64-65	0.11111456209317794
1106	66-67	0.1649797314963095
1106	68-69	0.15092446239033563
1106	70-71	0.14106399411595305
1106	72-73	0.10746081279191344
1106	74-75	0.2077066523886586
1106	76-77	0.12299750295188971
1106	78-79	0.1871232784922796
1106	80-81	0.19766935813356667
1106	82-83	0.18841653617954535
1106	84-85	0.19341791648541573
1106	86-87	0.15115654991910787
1106	88-89	0.17869717380298766
1106	90-91	0.12501417122294356
1106	92-93	0.19655568126227507
1106	94-95	0.13893793000040944
1106	96-97	0.14020352403805703
1106	98-99	0.18752968286085547
1106	100	0.17072901844298372
1107	1	-0.027284856959326476
1107	2	0.004951862602084134
1107	3	0.0159480997506094
1107	4	-0.003635093377468479
1107	5	0.04329018322710709
1107	6	0.05885216345389921
1107	7	0.057068135825858235
1107	8	0.03587740100090997
1107	9	0.05780339365362863
1107	10-11	0.07736096803660075
1107	12-13	0.06501440087708943
1107	14-15	0.09944297468630481
1107	16-17	0.10760039055185189
1107	18-19	0.12436621072853171
1107	20-21	0.09391065692101108
1107	22-23	0.08146436864551276
1107	24-25	0.12603649796142236
1107	26-27	0.09367892011465528
1107	28-29	0.10736937100679
1107	30-31	0.1014765571071834
1107	32-33	0.07489492551758303
1107	34-35	0.060211330619580394
1107	36-37	0.039934740826382154
1107	38-39	0.0808614618245258
1107	40-41	0.05557388276912434
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2216	26-27	-0.3543360288273334
2216	28-29	-0.3237256074119941
2216	30-31	-0.31627609053994377
2216	32-33	-0.3699129068858298
2216	34-35	-0.37077883676646195
2216	36-37	-0.29722597082747626
2216	38-39	-0.321077100129358
2216	40-41	-0.3251539412123421
2216	42-43	-0.33193066370858304
2216	44-45	-0.3481996745589626
2216	46-47	-0.24919319875395018
2216	48-49	-0.24318403800639032
2216	50-51	-0.30979812500952875
2216	52-53	-0.32200076531724875
2216	54-55	-0.3248824642661958
2216	56-57	-0.308431695084586
2216	58-59	-0.2837973732927779
2216	60-61	-0.2943877418958678
2216	62-63	-0.3679811107408355
2216	64-65	-0.4528959182561678
2216	66-67	-0.42361865514941854
2216	68-69	-0.4146576143192817
2216	70-71	-0.42317621067248723
2216	72-73	-0.3636196269008991
2216	74-75	-0.37982575021851517
2216	76-77	-0.3618949702126457
2216	78-79	-0.42674801455739697
2216	80-81	-0.37807914790037955
2216	82-83	-0.37533431889821145
2216	84-85	-0.44445766163320144
2216	86-87	-0.45389768029578903
2216	88-89	-0.589170686028794
2216	90-91	-0.5795620931090184
2216	92-93	-0.5856692762527231
2216	94-95	-0.4311850721292174
2216	96-97	-0.5528189725738955
2216	98-99	-0.6121620589164145
2216	100	-0.6061827375318067
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29.0
3	5.0
4	14.0
5	36.0
6	112.0
7	242.0
8	572.0
9	1043.0
10	1884.0
11	3056.0
12	4584.0
13	6891.0
14	7949.0
15	10470.0
16	12788.0
17	18150.0
18	21063.0
19	11635.0
20	8105.0
21	8046.0
22	8745.0
23	9377.0
24	10787.0
25	13114.0
26	16643.0
27	21554.0
28	27965.0
29	35953.0
30	45915.0
31	59140.0
32	76289.0
33	99369.0
34	138295.0
35	215375.0
36	409851.0
37	920290.0
38	1580132.0
39	709778.0
40	1793.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81346642684497	19.537893622200205	13.547511739148058	24.101128211806767
2	15.570861690207671	20.68001462015233	39.68110846852573	24.06801522111427
3	17.849517546500795	25.78925602269869	30.489170477529782	25.872055953270728
4	12.359434145641655	17.12210217488812	38.64762331516904	31.870840364301177
5	12.86070999322785	37.83894236676364	35.623946625093616	13.676401014914891
6	30.58663664109091	38.20853399949259	17.852738896595515	13.352090462820989
7	27.044619273820746	32.76679701016529	22.794246407879143	17.39433730813482
8	25.394109739704263	35.72927348497327	20.59315264767714	18.28346412764533
9	25.76397945645366	15.916178717961035	20.885318900279586	37.434522925305714
10-11	24.102228108113852	26.31890168199537	28.97061909898039	20.608251110910388
12-13	25.057122597347508	23.800137656548902	29.161791607289643	21.980948138813943
14-15	22.257968428145087	25.397686687678263	26.508117779616008	25.83622710456064
16-17	21.613190168497848	28.836928504586307	26.985878556069242	22.564002770846603
18-19	21.481908673044405	27.566173438749615	29.367784570000637	21.58413331820534
20-21	22.62648163985301	26.77521712785743	29.043849300393465	21.554451931896093
22-23	22.351991567913572	26.594290962991955	29.007280555825215	22.046436913269257
24-25	22.141129617414432	26.64583346939372	28.986939199216643	22.226097713975204
26-27	21.923220609991183	26.790664003413745	29.035323522688845	22.250791863906226
28-29	21.89822083099173	26.887478693108708	29.04626707249429	22.168033403405275
30-31	22.138045833595303	27.04537887234018	28.781019234737116	22.035556059327398
32-33	22.07577795985379	26.881038662716882	29.06510216095101	21.97808121647832
34-35	22.107856496257835	26.670690689188202	28.984772989562412	22.236679824991548
36-37	22.364285541922484	26.863361153180215	28.646188354805	22.126164950092306
38-39	22.151801213139848	27.121771585323927	28.650516411303954	22.07591079023227
40-41	22.108475091082937	26.86599644855687	28.701281393090206	22.324247067269983
42-43	22.189071203503	26.768464916951128	28.597860235432993	22.444603644112878
44-45	22.29764896871601	27.088242983954753	28.40386146765613	22.2102465796731
46-47	22.367440263411495	27.149046089706108	28.208268735337462	22.27524491154493
48-49	22.400138674915137	27.161465730094424	27.863607110764377	22.57478848422606
50-51	22.347991682161698	27.218793107608768	27.82463246387733	22.6085827463522
52-53	22.39670722347095	27.1603256026791	27.883155314798035	22.559811859051916
54-55	22.46410757135371	27.368326906187878	27.689167616219386	22.47839790623902
56-57	22.270905785847763	27.192592315452668	27.828030707726896	22.70847119097267
58-59	22.160568018119832	26.876389599469917	28.140702349481593	22.82234003292865
60-61	22.441924898146773	27.235131244162382	27.68141917747445	22.6415246802164
62-63	22.239314294164828	27.156672767270774	27.826337120401217	22.777675818163182
64-65	22.309791879149152	26.936517484130647	27.891523628642567	22.862167008077634
66-67	22.140344592995547	27.081025866723756	27.847589980958766	22.931039559321935
68-69	22.434998487390885	27.131458849228235	27.726340950396715	22.707201712984162
70-71	22.412490754448104	26.939507026398058	27.76393020468949	22.884072014464348
72-73	22.290935105620967	27.068438526290922	27.71922091633552	22.921405451752584
74-75	22.383210394965015	27.153996882466874	27.527704579532625	22.935088143035482
76-77	22.38554947167824	27.033472591226243	27.571192101728588	23.00978583536693
78-79	22.45566177312173	27.158997298894256	27.572597889900884	22.812743038083134
80-81	22.307024579597385	27.147795270308713	27.61191568193235	22.933264468161553
82-83	22.22926346920751	26.984750725282264	27.775479686949478	23.010506118560752
84-85	22.221249362690912	26.95524656749698	27.615723486115574	23.207780583696533
86-87	22.31702300810978	27.093948748465802	27.51181310847692	23.077215134947497
88-89	22.03411894374801	27.08275426237734	27.560877307580462	23.322249486294186
90-91	22.014379665498765	26.94772604603034	27.619121731687912	23.418772556782987
92-93	22.134738068347666	26.97013963998518	27.54446886695033	23.35065342471682
94-95	21.9169431490645	26.795165257937516	27.64805160576748	23.639839987230506
96-97	21.899728992190333	26.782784301670887	27.692951239957864	23.62453546618092
98-99	21.85556073471671	26.893214318645192	27.561097344086555	23.69012760255154
100	21.9394607839339	26.754517727210235	27.54237897879562	23.76364251006024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	56.0
1	50.5
2	164.0
3	275.0
4	180.0
5	152.0
6	340.0
7	805.5
8	1407.0
9	1622.5
10	1315.0
11	993.5
12	1068.0
13	1287.5
14	1534.5
15	1793.5
16	2062.0
17	2361.0
18	2773.0
19	3200.0
20	3721.5
21	4625.0
22	5907.5
23	8028.0
24	10786.0
25	14400.0
26	19601.0
27	26542.0
28	36205.5
29	48339.5
30	60233.5
31	72896.5
32	86509.5
33	100643.5
34	114681.0
35	124814.5
36	132232.0
37	141057.0
38	148539.0
39	152186.0
40	156535.5
41	161816.0
42	162861.5
43	161739.0
44	160585.0
45	158607.0
46	157261.5
47	152270.0
48	148150.0
49	146956.0
50	144245.5
51	141080.0
52	134586.0
53	130644.0
54	128483.0
55	121608.0
56	111383.0
57	100071.0
58	91907.5
59	85716.0
60	77719.0
61	67773.5
62	58208.5
63	49474.0
64	41009.5
65	32667.0
66	25403.5
67	19242.5
68	14431.5
69	11360.0
70	8302.0
71	5741.0
72	4266.5
73	3119.5
74	2275.0
75	1536.0
76	1004.0
77	702.0
78	454.5
79	242.5
80	114.0
81	59.0
82	31.0
83	12.0
84	6.5
85	5.0
86	3.5
87	2.5
88	2.5
89	2.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.044675283963676204
2	0.24197267280623436
3	0.00281157634459211
4	0.0025901923804509987
5	2.2138396414111104E-4
6	2.21383964141111E-5
7	0.0
8	5.091831175245554E-4
9	0.0
10-11	5.091831175245554E-4
12-13	0.0
14-15	2.1031476593405546E-4
16-17	1.771071713128888E-4
18-19	5.866675049739443E-4
20-21	0.0
22-23	1.6603797310583328E-4
24-25	0.0010958506224984996
26-27	1.4389957669172217E-4
28-29	3.21006748004611E-4
30-31	6.64151892423333E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	4.42767928282222E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.1031476593405546E-4
70-71	4.42767928282222E-5
72-73	3.21006748004611E-4
74-75	1.328303784846666E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	9.962278386349996E-5
84-85	0.0
86-87	5.091831175245554E-4
88-89	0.0023356008216887216
90-91	0.002324531623481666
92-93	6.752210906303887E-4
94-95	5.423907121457221E-4
96-97	2.3245316234816657E-4
98-99	1.2176118027761106E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4517039.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.761110094333056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.24098588374582	30.97502033667
2	16.37280303700701	15.31220890732631
3	6.757161918878804	9.479171772417798
4	3.387978390360529	6.3370252203548
5	1.9969237157187403	4.668918486035434
6	1.2721875593624472	3.5693341514392953
7	0.8536886187100962	2.7943599240047345
8	0.5646299027098646	2.112217683453484
9	0.43049611098973817	1.8117428437056031
>10	1.9846397640123854	16.090747554231104
>50	0.09896333464419246	3.1532609029132512
>100	0.0384531949581253	3.040896883745895
>500	8.519235892980632E-4	0.2621046001212182
>1k	1.4198718777860096E-4	0.127278803222647
>5k	9.465812518573376E-5	0.26571193035838947
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6660	0.14744172011797993	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5346	0.11835186722983794	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.64151892423333E-5	0.0
4	0.0	0.0	0.0	1.328303784846666E-4	0.0
5	0.0	0.0	0.0	1.328303784846666E-4	0.0
6	0.0	0.0	0.0	1.328303784846666E-4	0.0
7	0.0	0.0	0.0	1.328303784846666E-4	0.0
8	0.0	0.0	0.0	1.9924556772699993E-4	0.0
9	0.0	0.0	0.0	3.542143426257776E-4	0.0
10-11	2.21383964141111E-5	0.0	0.0	3.9849113545399985E-4	0.0
12-13	2.21383964141111E-5	0.0	0.0	4.4276792828222203E-4	0.0
14-15	2.21383964141111E-5	0.0	0.0	4.4276792828222203E-4	0.0
16-17	2.21383964141111E-5	0.0	0.0	4.538371264892776E-4	0.0
18-19	2.21383964141111E-5	0.0	0.0	4.759755229033887E-4	0.0
20-21	2.21383964141111E-5	0.0	0.0	5.534599103527776E-4	0.0
22-23	2.21383964141111E-5	0.0	0.0	5.755983067668887E-4	0.0
24-25	2.21383964141111E-5	0.0	0.0	7.084286852515552E-4	0.0
26-27	2.21383964141111E-5	0.0	0.0	8.63397460150333E-4	0.0
28-29	2.21383964141111E-5	0.0	0.0	0.0014389957669172218	0.0
30-31	6.64151892423333E-5	0.0	0.0	0.005567806698148942	0.0
32-33	6.64151892423333E-5	0.0	0.0	0.014412096065586327	0.0
34-35	6.64151892423333E-5	0.0	0.0	0.02849211618496099	0.0
36-37	6.64151892423333E-5	0.0	0.0	0.05280007544765498	0.0
38-39	6.64151892423333E-5	0.0	0.0	0.0927377425787114	0.0
40-41	6.64151892423333E-5	0.0	0.0	0.14113227713995827	0.0
42-43	6.64151892423333E-5	0.0	0.0	0.192283042054762	0.0
44-45	6.64151892423333E-5	0.0	0.0	0.2525880338868006	0.0
46-47	6.64151892423333E-5	0.0	0.0	0.32681807706331517	0.0
48-49	8.85535856564444E-5	0.0	0.0	0.40815454548875935	0.0
50-51	8.85535856564444E-5	0.0	0.0	0.4957561800993969	0.0
52-53	9.962278386349996E-5	0.0	0.0	0.5852727859998552	0.0
54-55	1.1069198207055551E-4	0.0	0.0	0.6854490297737079	0.0
56-57	1.1069198207055551E-4	0.0	0.0	0.8034688210573342	0.0
58-59	1.1069198207055551E-4	0.0	0.0	0.9366534138846266	0.0
60-61	1.2176118027761105E-4	0.0	0.0	1.0822687162984423	0.0
62-63	1.328303784846666E-4	0.0	0.0	1.2334961022032354	0.0
64-65	1.328303784846666E-4	0.0	0.0	1.3924055116637248	0.0
66-67	1.328303784846666E-4	0.0	0.0	1.5762870322793316	0.0
68-69	1.328303784846666E-4	0.0	0.0	1.7781449307831967	0.0
70-71	1.328303784846666E-4	0.0	0.0	1.9988093970408491	0.0
72-73	1.328303784846666E-4	0.0	0.0	2.2296575256489923	0.0
74-75	1.328303784846666E-4	0.0	0.0	2.46973736556182	0.0
76-77	1.328303784846666E-4	0.0	0.0	2.7294207554993437	0.0
78-79	1.5496877489877772E-4	0.0	0.0	3.0271157720798954	0.0
80-81	1.6603797310583328E-4	0.0	0.0	3.3533029048454086	0.0
82-83	1.771071713128888E-4	0.0	0.0	3.6935811269285033	0.0
84-85	1.771071713128888E-4	0.0	0.0	4.0414528189816386	0.0
86-87	1.771071713128888E-4	0.0	0.0	4.4100903268712095	0.0
88	1.771071713128888E-4	0.0	0.0	4.707331506325272	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5635	0.0	51.86977	1
GTATCAA	10800	0.0	47.31753	1
TCAACGC	13090	0.0	38.5263	4
ATCAACG	13335	0.0	37.820145	3
CAACGCA	13635	0.0	36.98474	5
TATCAAC	13970	0.0	36.423256	2
AACGCAG	14240	0.0	35.446415	6
ACGCAGA	16170	0.0	30.89593	7
CGCAGAG	16480	0.0	30.058424	8
GTACATG	13695	0.0	27.504343	1
TACATGG	13850	0.0	26.29548	2
GCAGAGT	18800	0.0	25.923817	9
ACATGGG	14440	0.0	24.607702	3
AGAGTAC	16975	0.0	21.290928	10-11
CATGGGG	11090	0.0	21.147852	4
GAGTACT	11730	0.0	21.054762	12-13
CAGAGTA	17950	0.0	20.802114	10-11
TACACCG	585	0.0	20.084553	5
TATACGC	150	8.298103E-4	18.799143	5
AGTACTT	12830	0.0	18.773397	12-13
>>END_MODULE