FastQCFastQC Report
Wed 25 May 2016
SRR1033030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140133
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34650.032.703951
TCAACGC32500.032.546364
ATCAACG33500.031.9958273
CAACGCA34800.030.3948235
GGTATCA24100.028.8756051
AACGCAG37300.028.7297826
TATCAAC43900.025.0584742
GTACATG81500.024.577541
ACGCAGA45350.023.4227287
TACATGG80700.023.2435592
ACATGGG80350.023.1107753
CGCAGAG45750.022.9283818
GTCTCGC5900.019.92391
GAGTACA57300.019.6944121
CATGGGG58300.018.949714
GTACGAC2001.4057683E-518.8045643
AGTACAT59300.017.916662
CTGTCGC10100.017.2343719
GCAGAGT59250.016.9124419
TACCGCG2002.9754243E-416.4637098
Produced by FastQC (version 0.11.5)