##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033030_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3140133
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.680100811016604	33.0	31.0	34.0	30.0	34.0
2	31.79922474621298	34.0	31.0	34.0	30.0	34.0
3	31.905148603578255	34.0	31.0	34.0	30.0	34.0
4	35.258767383419745	37.0	35.0	37.0	33.0	37.0
5	35.27554215060317	37.0	35.0	37.0	33.0	37.0
6	35.3838025332048	37.0	36.0	37.0	33.0	37.0
7	35.30189390067236	37.0	36.0	37.0	33.0	37.0
8	35.295865175137486	37.0	36.0	37.0	33.0	37.0
9	36.941589098296156	39.0	38.0	39.0	34.0	39.0
10-11	36.93341078228215	39.0	38.0	39.0	34.0	39.0
12-13	36.861677514933284	39.0	38.0	39.0	34.0	39.0
14-15	38.275017013610565	41.0	39.0	41.0	34.0	41.0
16-17	38.27635167045472	41.0	39.0	41.0	34.0	41.0
18-19	38.284701316791356	41.0	39.0	41.0	34.0	41.0
20-21	38.30663255346191	41.0	39.0	41.0	34.0	41.0
22-23	38.270490135290444	40.5	39.0	41.0	34.0	41.0
24-25	38.2837793813192	40.5	39.0	41.0	34.0	41.0
26-27	38.19759354142006	40.0	38.0	41.0	33.5	41.0
28-29	38.205245287381146	40.0	38.5	41.0	33.5	41.0
30-31	38.35181280538117	41.0	39.0	41.0	34.0	41.0
32-33	38.34011170864419	41.0	39.0	41.0	34.0	41.0
34-35	38.295563754783636	41.0	39.0	41.0	33.5	41.0
36-37	38.22075991685703	41.0	38.5	41.0	33.0	41.0
38-39	38.13435832176535	41.0	38.0	41.0	33.0	41.0
40-41	38.06680545059716	40.5	38.0	41.0	33.0	41.0
42-43	37.95177465413089	40.0	38.0	41.0	33.0	41.0
44-45	37.82283075271016	40.0	38.0	41.0	33.0	41.0
46-47	37.69900526506362	40.0	38.0	41.0	32.0	41.0
48-49	37.58470310015531	40.0	38.0	41.0	32.0	41.0
50-51	37.46757971716485	40.0	37.5	41.0	32.0	41.0
52-53	37.31362158863972	40.0	37.0	41.0	31.5	41.0
54-55	37.15780748777202	40.0	37.0	41.0	31.0	41.0
56-57	36.987888570324884	40.0	36.0	41.0	31.0	41.0
58-59	36.791553892780975	40.0	36.0	41.0	31.0	41.0
60-61	36.56932604447009	39.0	35.5	41.0	31.0	41.0
62-63	36.31744833737934	39.0	35.0	41.0	30.0	41.0
64-65	36.034193137679196	39.0	35.0	41.0	30.0	41.0
66-67	35.73113973197951	38.0	35.0	40.0	30.0	41.0
68-69	35.4100100218685	37.5	35.0	40.0	29.5	41.0
70-71	35.06375717206883	37.0	35.0	40.0	29.0	41.0
72-73	34.706365462864156	36.5	34.0	39.0	29.0	41.0
74-75	34.34499780741771	36.0	34.0	39.0	29.0	41.0
76-77	33.980429969049084	35.5	34.0	38.5	28.5	40.0
78-79	33.593590303340655	35.0	34.0	37.0	28.0	39.0
80-81	33.2455225622609	35.0	34.0	37.0	27.5	39.0
82-83	33.02692640725727	35.0	34.0	36.5	28.0	39.0
84-85	32.807119475512664	35.0	34.0	36.0	27.5	37.0
86-87	32.63462168640628	35.0	34.0	36.0	28.0	37.0
88-89	32.42364399851853	35.0	34.0	35.5	27.0	37.0
90-91	32.22259375637911	35.0	34.0	35.0	27.0	36.0
92-93	32.0112106716499	35.0	33.0	35.0	27.0	36.0
94-95	31.822690790485623	35.0	33.0	35.0	26.0	36.0
96-97	31.658677833072677	35.0	33.0	35.0	26.0	36.0
98-99	31.49538045044589	35.0	33.0	35.0	25.0	35.0
100	31.364890913856197	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.07355767992962114
1101	2	0.11975090797734822
1101	3	0.045130484760630196
1101	4	-0.09904571802075424
1101	5	-0.01678392694987707
1101	6	0.007634873165002887
1101	7	0.053533661090909845
1101	8	0.15047860944799396
1101	9	0.09190850700667852
1101	10-11	0.11772467909107576
1101	12-13	0.09470027849145879
1101	14-15	0.10592795098263252
1101	16-17	0.12342467374995181
1101	18-19	0.13571142725802332
1101	20-21	0.1880783182579009
1101	22-23	0.18125731561772795
1101	24-25	0.15857643753950157
1101	26-27	0.16366860815386985
1101	28-29	0.18530191225499237
1101	30-31	0.285852553787862
1101	32-33	0.23966166570588854
1101	34-35	0.2556063108498705
1101	36-37	0.2668455013653741
1101	38-39	0.2927859288310444
1101	40-41	0.2785117277622078
1101	42-43	0.34370666511366466
1101	44-45	0.3042279457339774
1101	46-47	0.2923377268374736
1101	48-49	0.3084129979560686
1101	50-51	0.3386740898835612
1101	52-53	0.30957994150311663
1101	54-55	0.23732504253657538
1101	56-57	0.30930473590984064
1101	58-59	0.42337955438319597
1101	60-61	0.3419870766417148
1101	62-63	0.3700646736364206
1101	64-65	0.33166643153205655
1101	66-67	0.3213333853937854
1101	68-69	0.305529727403659
1101	70-71	0.22885628139752612
1101	72-73	0.17347108348452167
1101	74-75	0.18496706244327044
1101	76-77	0.21921974770307173
1101	78-79	0.16206816651612144
1101	80-81	0.1888180618616957
1101	82-83	0.0887600989149675
1101	84-85	0.08217933602228555
1101	86-87	0.11812004708922075
1101	88-89	0.18426110605207668
1101	90-91	0.24214865829058851
1101	92-93	0.2277424220837645
1101	94-95	0.2402742756482823
1101	96-97	0.16392269963506578
1101	98-99	0.19993376519238382
1101	100	0.15849035667740452
1102	1	-0.08870875480054252
1102	2	0.019168344353033717
1102	3	0.017525666586934108
1102	4	-0.07750532505033192
1102	5	-0.07563304443706897
1102	6	0.010203963158602392
1102	7	0.06709105699869866
1102	8	0.0918297722018977
1102	9	0.20012341730346606
1102	10-11	0.15659911460677023
1102	12-13	0.09275174894282401
1102	14-15	0.17271088256880773
1102	16-17	0.17482968355978556
1102	18-19	0.19992596689385067
1102	20-21	0.22949406746305812
1102	22-23	0.16726495151279153
1102	24-25	0.15927069971710495
1102	26-27	0.022361299966846104
1102	28-29	0.08766863951181136
1102	30-31	0.1944309933726842
1102	32-33	0.20250557997872676
1102	34-35	0.1419530038001966
1102	36-37	0.18504832853358977
1102	38-39	0.24941513500696288
1102	40-41	0.1677370056299594
1102	42-43	0.1390743279811133
1102	44-45	0.14518862309652292
1102	46-47	0.11696393049670917
1102	48-49	8.91384210390811E-4
1102	50-51	0.032420239011656804
1102	52-53	0.015153968058541523
1102	54-55	0.10983352259859913
1102	56-57	0.12725898406142022
1102	58-59	0.05472078281831472
1102	60-61	-0.006031835909290351
1102	62-63	0.03524450819086411
1102	64-65	0.058221689333393556
1102	66-67	0.06662205885101713
1102	68-69	0.0595113951592694
1102	70-71	0.060999072891078754
1102	72-73	0.06580064691896581
1102	74-75	0.10792440758005029
1102	76-77	0.06078420338756274
1102	78-79	0.07604960624427548
1102	80-81	0.12291193076173101
1102	82-83	0.05876730388466456
1102	84-85	0.06611933874000187
1102	86-87	0.1051899012074955
1102	88-89	0.1378208124830138
1102	90-91	0.11315666531197621
1102	92-93	0.12750725131446927
1102	94-95	0.20700780322378165
1102	96-97	0.15117462669978465
1102	98-99	0.14459664869734468
1102	100	0.08867823734365388
1103	1	-0.0942682080268149
1103	2	-0.034471827715684356
1103	3	0.0010987328251097495
1103	4	-0.09464424678996153
1103	5	-0.15855464442878997
1103	6	-0.10930126411616925
1103	7	-0.05151936713303229
1103	8	-0.07075133344329743
1103	9	0.04389800201982297
1103	10-11	0.026352835597457158
1103	12-13	0.05801006704660949
1103	14-15	0.15218943975408195
1103	16-17	0.20272873699021687
1103	18-19	0.11633835803966974
1103	20-21	0.05425559967220295
1103	22-23	0.11131153435870544
1103	24-25	0.03403171156379159
1103	26-27	0.03680934216234988
1103	28-29	-0.02052093474856065
1103	30-31	0.0087293033400897
1103	32-33	0.024709076494978888
1103	34-35	-0.0188370914063114
1103	36-37	0.0508647229681074
1103	38-39	0.08901982694715826
1103	40-41	0.13995994828342617
1103	42-43	0.12556571895617452
1103	44-45	0.10689749115733349
1103	46-47	0.09729343170480576
1103	48-49	0.015168284980134672
1103	50-51	0.04608060664965308
1103	52-53	0.05642157317272023
1103	54-55	0.06568823057642703
1103	56-57	0.07109833049940306
1103	58-59	0.07307451683733035
1103	60-61	0.004865407155421053
1103	62-63	-0.032376123249413524
1103	64-65	-0.04780232365882853
1103	66-67	0.033690026831266096
1103	68-69	-0.03171601539771274
1103	70-71	0.049492675378999706
1103	72-73	-0.031130101209697614
1103	74-75	-0.009397114572060161
1103	76-77	-0.013338811064912193
1103	78-79	0.04116351688697506
1103	80-81	0.10128924683576912
1103	82-83	0.045305747738844104
1103	84-85	0.017556830352937425
1103	86-87	0.029274582293318474
1103	88-89	0.02566635172965448
1103	90-91	0.06739712088736383
1103	92-93	0.07720092302986359
1103	94-95	0.06118333188311453
1103	96-97	0.0605552968263936
1103	98-99	0.0016537463336909752
1103	100	-0.041072458386437916
1104	1	-0.029183399160768175
1104	2	0.10112314589210669
1104	3	0.11886922188877591
1104	4	0.10781468940916739
1104	5	0.029782014483188846
1104	6	0.13072297832089674
1104	7	0.1897044634364491
1104	8	0.24579122118283436
1104	9	0.29571430738567983
1104	10-11	0.22084733265943868
1104	12-13	0.3061118694167959
1104	14-15	0.3887120144658951
1104	16-17	0.34586985663753467
1104	18-19	0.3537593413920277
1104	20-21	0.2867420254411073
1104	22-23	0.27405494371054573
1104	24-25	0.2914080441971123
1104	26-27	0.21265858106934132
1104	28-29	0.1827301114469222
1104	30-31	0.2121982881986213
1104	32-33	0.20931370106772818
1104	34-35	0.24038936648487663
1104	36-37	0.25586956458047183
1104	38-39	0.25412684747020364
1104	40-41	0.3473689373410096
1104	42-43	0.3214380791404139
1104	44-45	0.3059232984585165
1104	46-47	0.24776966020775149
1104	48-49	0.2751243958036085
1104	50-51	0.28989032361496925
1104	52-53	0.23976008041186958
1104	54-55	0.2575225115189852
1104	56-57	0.27646469327182643
1104	58-59	0.31055703176806304
1104	60-61	0.3344467068440906
1104	62-63	0.33886562472337545
1104	64-65	0.3602072527384337
1104	66-67	0.36412872292238774
1104	68-69	0.28575115233572745
1104	70-71	0.19009719735504405
1104	72-73	0.22366449649224052
1104	74-75	0.2453271726585129
1104	76-77	0.2498858772976078
1104	78-79	0.20658874583892128
1104	80-81	0.19538488599999937
1104	82-83	0.2061807336582504
1104	84-85	0.21607007722651872
1104	86-87	0.24605945552028174
1104	88-89	0.23270274024115167
1104	90-91	0.14312186874539634
1104	92-93	0.1375726298211788
1104	94-95	0.1985876829351767
1104	96-97	0.20665781208500889
1104	98-99	0.16508439492994142
1104	100	0.17988646115394857
1105	1	-0.023941337907828597
1105	2	0.10733969030255608
1105	3	0.14701951073107367
1105	4	0.1281341874504065
1105	5	0.04040442598076055
1105	6	0.11760207841285109
1105	7	0.08715722058583708
1105	8	0.09648756177347906
1105	9	0.2073529915608816
1105	10-11	0.1751037567887508
1105	12-13	0.22032328401224532
1105	14-15	0.19818279157937013
1105	16-17	0.19181904906010772
1105	18-19	0.22085948180077963
1105	20-21	0.22668866809730304
1105	22-23	0.24875401374231387
1105	24-25	0.18683870337127217
1105	26-27	0.17515662533999432
1105	28-29	0.1677406950971303
1105	30-31	0.19382952293761946
1105	32-33	0.14185496821884414
1105	34-35	0.17480147171775684
1105	36-37	0.2250182999427892
1105	38-39	0.2963302756240225
1105	40-41	0.274186604819576
1105	42-43	0.2655015951733546
1105	44-45	0.2734620269467527
1105	46-47	0.21657201697518502
1105	48-49	0.20945075176754813
1105	50-51	0.14573762632351617
1105	52-53	0.2117784132926488
1105	54-55	0.09731168641821597
1105	56-57	0.21936239272846336
1105	58-59	0.1672329076067598
1105	60-61	0.18394619154095437
1105	62-63	0.1810991837268503
1105	64-65	0.10888401887559951
1105	66-67	0.1935316374890661
1105	68-69	0.20960857739469674
1105	70-71	0.13300610124824175
1105	72-73	0.1701637157146152
1105	74-75	0.12203128165850075
1105	76-77	0.13304828409133762
1105	78-79	0.19757608886325073
1105	80-81	0.24823313452449725
1105	82-83	0.22101641706326092
1105	84-85	0.2679138126847249
1105	86-87	0.17960094575072105
1105	88-89	0.1578718414333622
1105	90-91	0.2296202703467145
1105	92-93	0.26809013347271105
1105	94-95	0.27081188472001827
1105	96-97	0.3183202678350554
1105	98-99	0.3194784014648562
1105	100	0.3474659697536069
1106	1	-0.185929762596043
1106	2	-0.17978603933338633
1106	3	-0.10653994669788247
1106	4	-0.19192378721718484
1106	5	-0.13146486084102094
1106	6	-0.13756447423394746
1106	7	-0.21449557062422997
1106	8	-0.08439574062418131
1106	9	-0.1356253343507845
1106	10-11	-0.07957206391844096
1106	12-13	-0.05747075611343888
1106	14-15	-0.11959585492118663
1106	16-17	-0.11286690325229642
1106	18-19	-0.04989214934992248
1106	20-21	-0.05035069007588078
1106	22-23	-0.08618704371049546
1106	24-25	-0.02066935380192092
1106	26-27	-0.010140198618742602
1106	28-29	-0.00647211502526801
1106	30-31	0.02229512623226526
1106	32-33	-0.03818978819434449
1106	34-35	-0.017536299019674573
1106	36-37	-0.016594872351475942
1106	38-39	0.015983193620662917
1106	40-41	-0.004724081590069318
1106	42-43	-0.005040281562180837
1106	44-45	-0.02104067160723133
1106	46-47	-0.07996346458548231
1106	48-49	-0.04160174655045523
1106	50-51	0.013258445168524702
1106	52-53	-0.03443048908659563
1106	54-55	-0.062113225180219445
1106	56-57	-0.040148109236916696
1106	58-59	-0.013845291405139903
1106	60-61	-0.02405104104033029
1106	62-63	-0.009665263810113345
1106	64-65	0.02214488450334784
1106	66-67	0.05546111208326465
1106	68-69	0.04436869984939307
1106	70-71	0.0907557038138549
1106	72-73	0.04195405337063818
1106	74-75	0.0012940554995637399
1106	76-77	0.012641797298080348
1106	78-79	0.04491836316523745
1106	80-81	0.08489967792644393
1106	82-83	0.10921580441245027
1106	84-85	0.11205972591214675
1106	86-87	0.09182115213359054
1106	88-89	0.06976291214970587
1106	90-91	0.1243509111509411
1106	92-93	0.10779356421913633
1106	94-95	0.053949450410364364
1106	96-97	0.12558468537341128
1106	98-99	0.08644836851921411
1106	100	0.02138440674690756
1107	1	-0.021980975703517203
1107	2	-0.02993364715967317
1107	3	-0.0035220968752369686
1107	4	-0.05587650816276124
1107	5	-0.0672622826171363
1107	6	-0.06338502967912518
1107	7	-0.047693337806613556
1107	8	-0.03467811222225947
1107	9	-0.0017921726906635627
1107	10-11	-4.0544365803185656E-4
1107	12-13	-0.003220209387848172
1107	14-15	0.062172485759901974
1107	16-17	0.04383083105649632
1107	18-19	0.05476035340840468
1107	20-21	0.082246433430754
1107	22-23	0.03516160716080208
1107	24-25	0.017061313740413198
1107	26-27	0.03227055496259368
1107	28-29	-0.0011789723851265421
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2216	42-43	-0.6544341037519601
2216	44-45	-0.5523312327601673
2216	46-47	-0.4929454000453717
2216	48-49	-0.5393090523345805
2216	50-51	-0.5198687104707318
2216	52-53	-0.5067041553124767
2216	54-55	-0.6568948577254261
2216	56-57	-0.6434759313349403
2216	58-59	-0.6055504467040862
2216	60-61	-0.5843312661589053
2216	62-63	-0.6944706602909392
2216	64-65	-0.6121667221349867
2216	66-67	-0.6770065246537342
2216	68-69	-0.6442342368434097
2216	70-71	-0.6640335248280778
2216	72-73	-0.649374810577342
2216	74-75	-0.6833533793369
2216	76-77	-0.5972629930630049
2216	78-79	-0.6888451978709753
2216	80-81	-0.5947776273408252
2216	82-83	-0.7577023813283645
2216	84-85	-0.5242676339157626
2216	86-87	-0.5191352156080811
2216	88-89	-0.5571313209145714
2216	90-91	-0.6065938766084287
2216	92-93	-0.7645627560178987
2216	94-95	-0.6595032630164326
2216	96-97	-0.6753779079154256
2216	98-99	-0.6550353501069637
2216	100	-0.5722061253108848
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31363.0
3	6716.0
4	3685.0
5	2948.0
6	2666.0
7	2952.0
8	3499.0
9	4009.0
10	4297.0
11	4710.0
12	5356.0
13	5996.0
14	6711.0
15	7170.0
16	7252.0
17	7044.0
18	6741.0
19	6390.0
20	6532.0
21	6708.0
22	7797.0
23	11177.0
24	17765.0
25	22748.0
26	22710.0
27	20048.0
28	22222.0
29	27492.0
30	33721.0
31	41278.0
32	53671.0
33	72833.0
34	103750.0
35	160127.0
36	294382.0
37	628474.0
38	1028122.0
39	438234.0
40	837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.001728361701744	19.67939272813482	13.310360738148102	24.008518172015325
2	15.447014861892939	20.09756492301438	40.39702016641217	24.05840004868051
3	18.310815504196086	25.15343795982796	29.830499725656562	26.705246810319395
4	12.203299841165027	16.678978714074105	39.24549563904944	31.87222580571143
5	13.025052996539568	37.85717085969614	35.78040133623631	13.33737480752798
6	30.956443411432875	38.26363320807785	17.830371836353372	12.949551544135904
7	27.257893830922526	32.4126148295009	22.660060052402414	17.66943128717416
8	24.78946524475014	36.831476240981125	20.4661427724822	17.912915741786538
9	24.664289079569052	17.25859829892441	21.154202929226408	36.92290969228013
10-11	23.631667367991533	27.181470997028935	29.018718998253064	20.168142636726472
12-13	24.31012875938741	24.4700060348491	28.85794386028224	22.361921345481242
14-15	21.6446450941171	26.49124006694061	25.92348182971206	25.940633009230236
16-17	21.499163098308223	29.815132686843242	26.460721711384284	22.22498250346425
18-19	21.384415956591045	28.836643743314777	28.291752582084722	21.48718771800946
20-21	22.66587935481866	28.086521428349442	27.742367338352047	21.50523187847985
22-23	22.155249107485396	27.879992633010488	27.780194141841314	22.184564117662802
24-25	22.085747503543615	28.001194476739556	27.8095844813582	22.103473538358628
26-27	21.87666545062019	27.867749442530158	27.915269369714125	22.340315737135523
28-29	21.92493541840572	27.933823961719323	27.939289079054436	22.201951540820524
30-31	22.167665483639034	28.47464968104147	27.531797054669134	21.825887780650362
32-33	21.764951929626918	28.07513749473166	28.06568834208801	22.094222233553413
34-35	21.92492290090098	27.840244168912804	28.09068484895202	22.144148081234196
36-37	22.279417492958746	28.204903123928123	27.463665797506014	22.05201358560712
38-39	22.11538522827882	28.346163333756145	27.680516098107592	21.857935339857438
40-41	21.922514137609785	28.036323211113885	27.956292690513912	22.084869960762415
42-43	21.851684002431053	27.880017197129263	27.97524518497909	22.29305361546059
44-45	21.983908399106884	28.043085156221615	27.891338032612804	22.081668412058693
46-47	21.988850400536844	28.21596369073638	27.81790485503019	21.97728105369659
48-49	21.926057845699226	28.11284792298331	27.761492897924427	22.199601333393037
50-51	21.962581602671776	27.99585320036082	27.772403418082142	22.269161778885262
52-53	21.99303668464729	27.875092483274926	27.856336883086957	22.275533948990823
54-55	22.073142361830346	28.382069413860467	27.6193040768135	21.92548414749569
56-57	21.87143424170059	28.10486374809806	27.747270125954476	22.276431884246872
58-59	21.76251332645746	27.704133603800734	28.08489863606865	22.44845443367316
60-61	22.01951261637727	28.118189102500267	27.722554577600278	22.139743703522186
62-63	21.916295347801118	28.13214447559748	27.680889278673664	22.270670897927733
64-65	21.92137994350935	27.836247544846042	27.855367750872638	22.387004760771966
66-67	21.75000904241394	27.924201148537943	27.753104336391182	22.572685472656936
68-69	22.209544277549156	28.039109680048934	27.671119987265758	22.080226055136155
70-71	22.076582098384037	27.884877350551406	27.768028900155596	22.27051165090896
72-73	21.90638001193961	28.034423803892146	27.84651913531988	22.212677048848363
74-75	21.96550644013831	28.067697212008508	27.708176861388132	22.25861948646505
76-77	21.94391108261794	28.06489921213979	27.634118814392423	22.357070890849847
78-79	22.146490377844604	28.121059007432248	27.645909524528705	22.086541090194444
80-81	22.073400964008737	27.85324777465065	27.83030808166977	22.243043179670842
82-83	21.96323487006219	27.980320303282213	27.893876716086822	22.162568110568774
84-85	22.00245159142373	27.82914685733538	27.729856608185834	22.438544943055057
86-87	22.089593607829645	28.03593047112946	27.66765747855896	22.206818442481936
88-89	21.880177164150194	27.909677760559504	27.7428774965082	22.467267578782103
90-91	21.871025662591336	27.812365398191975	27.82278310025716	22.49382583895953
92-93	22.09086849938854	27.99463363589487	27.653676448450774	22.26082141626581
94-95	21.88148424729129	27.763625421610477	27.796710713485833	22.5581796176124
96-97	21.87284100338494	27.74974030312332	27.85643864264037	22.520980050851364
98-99	21.86330642553404	27.850289112552385	27.75351470317895	22.53288975873463
100	22.051257541505162	27.779503674567025	27.604386220652454	22.56485256327536
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	448.0
1	423.5
2	380.5
3	359.5
4	367.0
5	375.0
6	360.5
7	325.0
8	289.0
9	281.0
10	268.5
11	254.5
12	274.5
13	300.0
14	320.0
15	390.0
16	502.5
17	614.0
18	825.0
19	1229.5
20	1749.0
21	2502.0
22	3721.5
23	5751.5
24	8610.0
25	12088.5
26	17074.0
27	23656.5
28	31565.0
29	40537.5
30	49846.5
31	58836.0
32	67255.0
33	76392.5
34	84983.0
35	91727.0
36	98208.5
37	104225.5
38	108549.5
39	111068.5
40	112771.0
41	114063.5
42	114197.5
43	113530.0
44	112002.5
45	110240.0
46	108422.0
47	105558.0
48	103501.5
49	101638.5
50	98628.5
51	95569.0
52	90182.0
53	85720.5
54	83188.5
55	78975.5
56	72484.5
57	66237.0
58	60209.0
59	53245.5
60	46840.5
61	40283.0
62	34218.5
63	28563.0
64	23142.5
65	18679.0
66	14982.5
67	11539.0
68	9045.5
69	7256.0
70	5564.0
71	3902.5
72	2790.0
73	2074.0
74	1495.0
75	955.5
76	542.5
77	373.0
78	268.0
79	179.5
80	105.5
81	63.5
82	44.0
83	25.5
84	18.5
85	17.0
86	14.0
87	11.5
88	12.0
89	8.0
90	3.5
91	2.5
92	3.0
93	3.5
94	2.0
95	1.0
96	1.0
97	3.0
98	4.0
99	2.5
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06053883704925875
2	0.041399520338788194
3	0.055125053620340286
4	0.07276761844163926
5	0.08483717090963981
6	0.08955034707128647
7	0.09149293994872192
8	0.10318034299821059
9	0.09741625593565623
10-11	0.10232050680655883
12-13	0.10674707090432156
14-15	0.10580762025047984
16-17	0.07544266437122249
18-19	0.09822832344999399
20-21	0.09157255441091189
22-23	0.05760902484066758
24-25	0.0217506710703018
26-27	0.18019937372079464
28-29	0.0652360903184674
30-31	0.07709864518477402
32-33	0.07278354133407726
34-35	0.021241138512285943
36-37	0.05300730892608689
38-39	0.07754448617303789
40-41	0.08216212498005658
42-43	0.07642988370237822
44-45	0.0747420571039507
46-47	0.08061760441357102
48-49	0.077178259646964
50-51	0.07297461604333319
52-53	0.07654134394944417
54-55	0.06824551698924855
56-57	0.0704906448230059
58-59	0.06714683741102687
60-61	0.06399410470830376
62-63	0.07066579663982385
64-65	0.066605459068135
66-67	0.06826143988168654
68-69	0.06807036517243059
70-71	0.05987007556686293
72-73	0.058580321279385304
74-75	0.06127129010140653
76-77	0.05601673559686803
78-79	0.05245000769075705
80-81	0.04676553509039267
82-83	0.05318246074290484
84-85	0.04316696139940569
86-87	0.04117659984465626
88-89	0.042959963797711755
90-91	0.039648002170608695
92-93	0.042116050498497995
94-95	0.040635221501764414
96-97	0.043867568666677494
98-99	0.044265640977627384
100	0.05311876917315285
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3140133.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.86189915419907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.99105978754386	47.769423369852944
2	14.028518955729712	17.637186877557028
3	4.694982002657066	8.854064555454242
4	1.9900044494127398	5.003818360615645
5	1.0219294523453744	3.2120213088020404
6	0.6006313256271917	2.265409549225766
7	0.38841141973485416	1.7091395648398182
8	0.2517449568931645	1.266013287423704
9	0.1787310934758584	1.0111838376419224
>10	0.8081895875027609	8.714762203748046
>50	0.03498335725328775	1.468956640435558
>100	0.010661803937491103	1.0336257317108857
>500	1.5180788657025897E-4	0.054394712692432144
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.184578487599092E-5	0.0
5	0.0	0.0	0.0	3.184578487599092E-5	0.0
6	0.0	0.0	0.0	3.184578487599092E-5	0.0
7	0.0	0.0	0.0	3.184578487599092E-5	0.0
8	0.0	0.0	0.0	6.369156975198184E-5	0.0
9	0.0	0.0	0.0	1.592289243799546E-4	0.0
10-11	0.0	0.0	0.0	1.7515181681795007E-4	0.0
12-13	0.0	0.0	0.0	1.9107470925594554E-4	0.0
14-15	0.0	0.0	0.0	3.343807411979047E-4	1.592289243799546E-5
16-17	0.0	0.0	0.0	4.2991809582587745E-4	3.184578487599092E-5
18-19	0.0	0.0	0.0	4.458409882638729E-4	3.184578487599092E-5
20-21	0.0	0.0	0.0	4.458409882638729E-4	3.184578487599092E-5
22-23	0.0	0.0	0.0	5.891470202058321E-4	3.184578487599092E-5
24-25	0.0	0.0	0.0	6.528385899578139E-4	3.184578487599092E-5
26-27	0.0	0.0	0.0	9.872193311557184E-4	3.184578487599092E-5
28-29	0.0	1.592289243799546E-5	0.0	0.001767441060617496	3.184578487599092E-5
30-31	0.0	3.184578487599092E-5	0.0	0.004538024344828706	3.184578487599092E-5
32-33	1.592289243799546E-5	3.184578487599092E-5	0.0	0.012388010316760468	3.184578487599092E-5
34-35	3.184578487599092E-5	3.184578487599092E-5	0.0	0.022403509660259613	3.184578487599092E-5
36-37	3.184578487599092E-5	3.184578487599092E-5	0.0	0.04116067695221826	3.184578487599092E-5
38-39	3.184578487599092E-5	3.184578487599092E-5	0.0	0.07319753653746514	3.184578487599092E-5
40-41	3.184578487599092E-5	3.184578487599092E-5	0.0	0.10923104212464886	3.184578487599092E-5
42-43	3.184578487599092E-5	3.184578487599092E-5	0.0	0.1480828996733578	3.184578487599092E-5
44-45	3.184578487599092E-5	3.184578487599092E-5	0.0	0.1946414371620565	3.184578487599092E-5
46-47	3.184578487599092E-5	3.184578487599092E-5	0.0	0.25037156069504063	3.184578487599092E-5
48-49	3.184578487599092E-5	3.184578487599092E-5	0.0	0.3148751979613602	3.184578487599092E-5
50-51	6.369156975198184E-5	3.184578487599092E-5	0.0	0.3843786234532104	3.184578487599092E-5
52-53	6.369156975198184E-5	3.184578487599092E-5	0.0	0.4547418851367124	3.184578487599092E-5
54-55	6.369156975198184E-5	3.184578487599092E-5	0.0	0.5307100049583886	3.184578487599092E-5
56-57	6.369156975198184E-5	3.184578487599092E-5	0.0	0.6279988777545409	3.184578487599092E-5
58-59	6.369156975198184E-5	3.184578487599092E-5	0.0	0.7400164260558391	3.184578487599092E-5
60-61	6.369156975198184E-5	3.184578487599092E-5	0.0	0.8542313335135805	3.184578487599092E-5
62-63	6.369156975198184E-5	3.184578487599092E-5	0.0	0.9728728050690847	3.184578487599092E-5
64-65	6.369156975198184E-5	3.184578487599092E-5	0.0	1.1035838290925892	3.184578487599092E-5
66-67	6.369156975198184E-5	3.184578487599092E-5	0.0	1.2523195673559049	3.184578487599092E-5
68-69	6.369156975198184E-5	3.184578487599092E-5	0.0	1.4198920873733691	3.184578487599092E-5
70-71	6.369156975198184E-5	3.184578487599092E-5	0.0	1.602925735948127	3.184578487599092E-5
72-73	6.369156975198184E-5	3.184578487599092E-5	0.0	1.7957041946949381	3.184578487599092E-5
74-75	6.369156975198184E-5	3.184578487599092E-5	0.0	1.9945492754606253	3.184578487599092E-5
76-77	6.369156975198184E-5	9.553735462797277E-5	0.0	2.2098108583298863	3.184578487599092E-5
78-79	6.369156975198184E-5	9.553735462797277E-5	0.0	2.4573959128482774	3.184578487599092E-5
80-81	6.369156975198184E-5	9.553735462797277E-5	0.0	2.7317951182322533	3.184578487599092E-5
82-83	6.369156975198184E-5	9.553735462797277E-5	0.0	3.0127704781931213	3.184578487599092E-5
84-85	6.369156975198184E-5	9.553735462797277E-5	0.0	3.293873221293493	3.184578487599092E-5
86-87	6.369156975198184E-5	9.553735462797277E-5	0.0	3.5941948955665257	3.184578487599092E-5
88	6.369156975198184E-5	9.553735462797277E-5	0.0	3.8491363263912706	3.184578487599092E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3465	0.0	32.70395	1
TCAACGC	3250	0.0	32.54636	4
ATCAACG	3350	0.0	31.995827	3
CAACGCA	3480	0.0	30.394823	5
GGTATCA	2410	0.0	28.875605	1
AACGCAG	3730	0.0	28.729782	6
TATCAAC	4390	0.0	25.058474	2
GTACATG	8150	0.0	24.57754	1
ACGCAGA	4535	0.0	23.422728	7
TACATGG	8070	0.0	23.243559	2
ACATGGG	8035	0.0	23.110775	3
CGCAGAG	4575	0.0	22.928381	8
GTCTCGC	590	0.0	19.9239	1
GAGTACA	5730	0.0	19.694412	1
CATGGGG	5830	0.0	18.94971	4
GTACGAC	200	1.4057683E-5	18.804564	3
AGTACAT	5930	0.0	17.91666	2
CTGTCGC	1010	0.0	17.234371	9
GCAGAGT	5925	0.0	16.912441	9
TACCGCG	200	2.9754243E-4	16.463709	8
>>END_MODULE