FastQCFastQC Report
Wed 25 May 2016
SRR1033030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140133
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54800.17451490112043025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46010.14652245621443424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44650.048.8648381
GTATCAA81700.043.8934331
TCAACGC91400.038.616624
ATCAACG91550.038.502013
CAACGCA93900.037.5884865
TATCAAC97000.036.6165242
AACGCAG97150.036.523956
ACGCAGA113150.031.1515927
CGCAGAG114450.030.6339878
GCAGAGT128050.027.0867759
GTACATG78550.025.0165141
TACATGG78400.024.0337432
ACATGGG77950.023.5744273
GAGTACT90150.023.14697612-13
CAGAGTA128000.021.42436410-11
AGAGTAC117900.021.1071310-11
GTGGTAT21500.020.8219811
GTACTTT101800.020.35953514-15
CATGGGG58800.019.9821954
AGTACTT96650.019.08599712-13
Produced by FastQC (version 0.11.5)